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Q99TG5 (KPYK_STAAM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
Ordered Locus Names:SAV1697
OrganismStaphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]
Taxonomic identifier158878 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Sequence similarities

Belongs to the pyruvate kinase family.

In the C-terminal section; belongs to the PEP-utilizing enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585Pyruvate kinase
PRO_0000294132

Sites

Metal binding341Potassium By similarity
Metal binding361Potassium By similarity
Metal binding661Potassium By similarity
Metal binding671Potassium; via carbonyl oxygen By similarity
Metal binding2211Magnesium By similarity
Metal binding2451Magnesium By similarity
Binding site321Substrate By similarity
Binding site2441Substrate; via amide nitrogen By similarity
Binding site2451Substrate; via amide nitrogen By similarity
Binding site2771Substrate By similarity
Site2191Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99TG5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 4AB45FDE55BF577D

FASTA58563,102
        10         20         30         40         50         60 
MRKTKIVCTI GPASESEEMI EKLINAGMNV ARLNFSHGSH EEHKGRIDTI RKVAKRLDKI 

        70         80         90        100        110        120 
VAILLDTKGP EIRTHNMKDG IIELERGNEV IVSMNEVEGT PEKFSVTYEN LINDVQVGSY 

       130        140        150        160        170        180 
ILLDDGLIEL QVKDIDHAKK EVKCDILNSG ELKNKKGVNL PGVRVSLPGI TEKDAEDIRF 

       190        200        210        220        230        240 
GIKENVDFIA ASFVRRPSDV LEIREILEEQ KANISVFPKI ENQEGIDNIA EILEVSDGLM 

       250        260        270        280        290        300 
VARGDMGVEI PPEKVPMVQK DLIRQCNKLG KPVITATQML DSMQRNPRAT RAEASDVANA 

       310        320        330        340        350        360 
IYDGTDAVML SGETAAGLYP EEAVKTMRNI AVSAEAAQDY KKLLSDRTKL VETSLVNAIG 

       370        380        390        400        410        420 
ISVAHTALNL NVKAIVAATE SGSTARTISK YRPHSDIIAV TPSEETARQC SIVWGVQPVV 

       430        440        450        460        470        480 
KKGRKSTDAL LNNAVATAVE TGRVSNGDLI IITAGVPTGE TGTTNMMKIH LVGDEIANGQ 

       490        500        510        520        530        540 
GIGRGSVVGT TLVAETVKDL EGKDLSDKVI VTNSIDETFV PYVEKALGLI TEENGITSPS 

       550        560        570        580 
AIVGLEKGIP TVVGVEKAVK NISNNMLVTI DAAQGKIFEG YANVL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000017 Genomic DNA. Translation: BAB57859.1.
RefSeqNP_372221.1. NC_002758.2.

3D structure databases

HSSPHSSP built from PDB template 1LIU based on UniProtKB P30613.
ProteinModelPortalQ99TG5.
SMRQ99TG5. Positions 1-583.
ModBaseSearch...

Protein-protein interaction databases

STRING158878.SAV1697.

2D gel databases

World-2DPAGE0002:Q99TG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB57859; BAB57859; SAV1697.
GeneID1121672.
KEGGsav:SAV1697.
PATRIC19564146. VBIStaAur52173_1751.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
KOK00873.
OMANSGYTAR.
ProtClustDBCLSK885504.

Enzyme and pathway databases

BioCycSAUR158878:GJJ5-1713-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF52009. PEP_mobile. 1 hit.
SSF50800. PK_B_barrel_like. 1 hit.
SSF52935. Pyruvate_kinase. 1 hit.
SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_STAAM
AccessionPrimary (citable) accession number: Q99TG5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 1, 2001
Last modified: May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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