Reviewed, UniProtKB/Swiss-Prot Q99S77 (PTLCB_STAAN)

Names and origin

Protein names

Recommended name:
    PTS system lactose-specific EIICB component
Alternative name(s):
    EIICB-Lac
      Short name=EII-Lac
Including the following 2 domains:
    1- Recommended name:
            Lactose permease IIC component
        Alternative name(s):
            PTS system lactose-specific EIIC component
    2- Recommended name:
            Lactose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system lactose-specific EIIB component

Gene names

Name:	lacE
Ordered Locus Names:	SA1992

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-3 domain. Contains 1 PTS EIIC type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	PTS system lactose-specific EIICB component		PRO_0000186587
Regions
	Transmembrane	30 – 50	21	Potential
	Transmembrane	61 – 81	21	Potential
	Transmembrane	102 – 122	21	Potential
	Transmembrane	131 – 151	21	Potential
	Transmembrane	174 – 194	21	Potential
	Transmembrane	219 – 239	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	334 – 354	21	Potential
	Transmembrane	375 – 395	21	Potential
	Domain	1 – 410	410	PTS EIIC type-3
	Domain	467 – 570	104	PTS EIIB type-3
Sites
	Active site	474	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99S77-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 11B6FCC1B716028E	FASTA	570	62,414
	10 20 30 40 50 60 MMQKLIAQIE KGKPFFEKLS RNIYLRAIRD GFISAMPVIL FSSIFLLIAY VPNIFGFKWD 70 80 90 100 110 120 KGMEAILMKP YNYTMGLVAF LVAGTTAKSL TDSFNRKLES TNQINFISTM LAAMCGFLFL 130 140 150 160 170 180 ASDPAKDGGF LSAFMGTKGL LTAFLSAFVT VIVYNFCVKR NITIKMPKEV PPNISQVFKD 190 200 210 220 230 240 LIPFSAVIII LYALDLVIRN SFKSNVAEGI LKLFEPLFTA ADGWIGVTII FGAFALFWFV 250 260 270 280 290 300 GIHGPSIVEP AIAAITYANI EANFKLLQAG EHADKIITSG TQMFIVTFGG TGATLVVPFM 310 320 330 340 350 360 FMWMTKSKRN KAIGRASVVP TFFGVNEPIL FGAPLVLNPV FFIPFVLAPI VNVWIFKLFV 370 380 390 400 410 420 EVLGINSFSV NLPWTTPGPL GIIMGTGFGL WSFVLAITLI VVDIIIYYPF LKVYDSEILD 430 440 450 460 470 480 EEEGRKESNS DLKEKVAANF DTKKADSILA ASGVSDDAAK ASNITEQTNV LVLCAGGGTS 490 500 510 520 530 540 GLLANALNKA AEEYHVPVKA AAGGYGAHMD IMKEYQLIIL APQVASNYED IKQDTDRLGI 550 560 570 KLAKTQGAEY IKLTRDGQAA LDFVQQQFEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB43282.1.
PIR	A90015.
RefSeq	NP_375303.1.
3D structure databases
SMR	Q99S77. Positions 466-567.
ModBase	Search...
Protein-protein interaction databases
STRING	Q99S77.
Genome annotation databases
GeneID	1124908.
GenomeReviews	Gene locus SA1992 in contig BA000018_GR.
KEGG	sau:SA1992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1455.
HOGENOM	HBG337916.
OMA	WSKDIEN.
Enzyme and pathway databases
BioCyc	SAUR158879:SA1992-MONOMER.
Family and domain databases
InterPro	IPR014350. PTrfase_system_EIIB_3_subgr. IPR003501. PTS_EIIB_2/3. IPR013012. PTS_EIIB_3. IPR003352. PTS_EIIC. IPR004501. PTS_IIC_lac. IPR004801. PTS_IIC_lac_spec. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00394. lac_pts_IIC. 1 hit. TIGR00410. lacE. 1 hit. TIGR00853. pts-lac. 1 hit.
PROSITE	PS51100. PTS_EIIB_TYPE_3. 1 hit. PS51105. PTS_EIIC_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTLCB_STAAN

Accession

Primary (citable) accession number: Q99S77

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)