Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q99S77 (PTLCB_STAAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PTS system lactose-specific EIICB component
Alternative name(s):
EIICB-Lac
Short name=EII-Lac

Including the following 2 domains:

  1. Lactose permease IIC component
    Alternative name(s):
    PTS system lactose-specific EIIC component
  2. Lactose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system lactose-specific EIIB component
Gene names
Name:lacE
Ordered Locus Names:SA1992
OrganismStaphylococcus aureus (strain N315) [Complete proteome] [HAMAP]
Taxonomic identifier158879 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-3 domain.

Contains 1 PTS EIIC type-3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570PTS system lactose-specific EIICB component
PRO_0000186587

Regions

Transmembrane30 – 5021Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane102 – 12221Helical; Potential
Transmembrane131 – 15121Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane219 – 23921Helical; Potential
Transmembrane278 – 29821Helical; Potential
Transmembrane334 – 35421Helical; Potential
Transmembrane375 – 39521Helical; Potential
Domain1 – 410410PTS EIIC type-3
Domain467 – 570104PTS EIIB type-3

Sites

Active site4741Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99S77 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 11B6FCC1B716028E

FASTA57062,414
        10         20         30         40         50         60 
MMQKLIAQIE KGKPFFEKLS RNIYLRAIRD GFISAMPVIL FSSIFLLIAY VPNIFGFKWD 

        70         80         90        100        110        120 
KGMEAILMKP YNYTMGLVAF LVAGTTAKSL TDSFNRKLES TNQINFISTM LAAMCGFLFL 

       130        140        150        160        170        180 
ASDPAKDGGF LSAFMGTKGL LTAFLSAFVT VIVYNFCVKR NITIKMPKEV PPNISQVFKD 

       190        200        210        220        230        240 
LIPFSAVIII LYALDLVIRN SFKSNVAEGI LKLFEPLFTA ADGWIGVTII FGAFALFWFV 

       250        260        270        280        290        300 
GIHGPSIVEP AIAAITYANI EANFKLLQAG EHADKIITSG TQMFIVTFGG TGATLVVPFM 

       310        320        330        340        350        360 
FMWMTKSKRN KAIGRASVVP TFFGVNEPIL FGAPLVLNPV FFIPFVLAPI VNVWIFKLFV 

       370        380        390        400        410        420 
EVLGINSFSV NLPWTTPGPL GIIMGTGFGL WSFVLAITLI VVDIIIYYPF LKVYDSEILD 

       430        440        450        460        470        480 
EEEGRKESNS DLKEKVAANF DTKKADSILA ASGVSDDAAK ASNITEQTNV LVLCAGGGTS 

       490        500        510        520        530        540 
GLLANALNKA AEEYHVPVKA AAGGYGAHMD IMKEYQLIIL APQVASNYED IKQDTDRLGI 

       550        560        570 
KLAKTQGAEY IKLTRDGQAA LDFVQQQFEN

« Hide

References

[1]"Whole genome sequencing of meticillin-resistant Staphylococcus aureus."
Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. expand/collapse author list , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K.
Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: N315.
[2]"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315."
Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.
Submitted (OCT-2007) to UniProtKB
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: N315.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000018 Genomic DNA. Translation: BAB43282.1.
PIRA90015.
RefSeqNP_375303.1. NC_002745.2.

3D structure databases

ProteinModelPortalQ99S77.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ99S77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000000264; EBSTAP00000000264; EBSTAG00000000264.
GeneID1124908.
GenomeReviewsGene locus SA1992 in contig BA000018_GR.
KEGGsau:SA1992.
PATRIC19576470. VBIStaAur116463_2153.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1455.
GeneTreeEBGT00050000025328.
HOGENOMHBG337916.
OMAWSKDIEN.
ProtClustDBCLSK877288.

Enzyme and pathway databases

BioCycSAUR158879:SA1992-MONOMER.

Family and domain databases

InterProIPR014350. PTrfase_system_EIIB_3_subgr.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_IIC_lac.
IPR004801. PTS_IIC_lac_spec.
[Graphical view]
KOK02787.
K02788.
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00394. Lac_pts_IIC. 1 hit.
TIGR00410. LacE. 1 hit.
TIGR00853. Pts-lac. 1 hit.
PROSITEPS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTLCB_STAAN
AccessionPrimary (citable) accession number: Q99S77
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents