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Protein

Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase

Gene

icaB

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. N-deacetylation is crucial for attachment of the polysaccharide to the bacterial cell surface; it leads to the introduction of positive charges in the otherwise neutral PIA polymer, allowing electrostatic interactions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-2736-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (EC:3.5.1.-)
Short name:
PNAG N-deacetylase
Short name:
Poly-beta-1,6-GlcNAc N-deacetylase
Alternative name(s):
Biofilm polysaccharide intercellular adhesin deacetylase
Short name:
Biofilm PIA deacetylase
Intercellular adhesion protein B
Gene namesi
Name:icaB
Ordered Locus Names:SAV2668
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 290262Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylasePRO_0000024835Add
BLAST

Proteomic databases

PaxDbiQ99QX2.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV2668.

Structurei

3D structure databases

ProteinModelPortaliQ99QX2.
SMRiQ99QX2. Positions 36-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 290177NodB homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polysaccharide deacetylase family.Curated
Contains 1 NodB homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107UVV. Bacteria.
COG0726. LUCA.
HOGENOMiHOG000280325.
OMAiLYHRFGP.
PhylomeDBiQ99QX2.

Family and domain databases

Gene3Di3.20.20.370. 1 hit.
InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. NODB_dom.
IPR023872. PNAG_deacetylase.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.
TIGRFAMsiTIGR03933. PIA_icaB. 1 hit.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99QX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYRKLIILV LSILIILPVS TLDGHHIANA DDDSPKKLKY KENSALALNY
60 70 80 90 100
HRVRKANFLN NFIYFFSSSK EIKNYSVSQS QFESQIKWLK SHDAKFLTLK
110 120 130 140 150
EFLYYKKKGK FPKRSVWINF DDMDETIYEN AYPILKKYKI PATGFIITGH
160 170 180 190 200
VGEENFHNLD MISKKELKEM YKTGLWEFET HTHDLHNLSK NNKSKLMKAS
210 220 230 240 250
EATIIKDLNK SEKYLTKNFK KSQKTIAYPY GLMNDDKLPV IKKAGLKYGF
260 270 280 290
SLEEKAVTPN SNDYYIPRIL ISDDAFEHLI KRWDGFHEKD
Length:290
Mass (Da):34,071
Last modified:June 1, 2001 - v1
Checksum:i70F7F2B8454F2E2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58830.1.
RefSeqiWP_000877369.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB58830; BAB58830; SAV2668.
KEGGisav:SAV2668.
PATRICi19566276. VBIStaAur52173_2764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58830.1.
RefSeqiWP_000877369.1. NC_002758.2.

3D structure databases

ProteinModelPortaliQ99QX2.
SMRiQ99QX2. Positions 36-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV2668.

Proteomic databases

PaxDbiQ99QX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB58830; BAB58830; SAV2668.
KEGGisav:SAV2668.
PATRICi19566276. VBIStaAur52173_2764.

Phylogenomic databases

eggNOGiENOG4107UVV. Bacteria.
COG0726. LUCA.
HOGENOMiHOG000280325.
OMAiLYHRFGP.
PhylomeDBiQ99QX2.

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-2736-MONOMER.

Family and domain databases

Gene3Di3.20.20.370. 1 hit.
InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. NODB_dom.
IPR023872. PNAG_deacetylase.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.
TIGRFAMsiTIGR03933. PIA_icaB. 1 hit.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICAB_STAAM
AccessioniPrimary (citable) accession number: Q99QX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.