Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99PW5

- NEUR3_RAT

UniProt

Q99PW5 - NEUR3_RAT

Protein

Sialidase-3

Gene

Neu3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.By similarity

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251SubstrateBy similarity
    Binding sitei45 – 451SubstrateBy similarity
    Active sitei50 – 501Proton acceptorBy similarity
    Binding sitei179 – 1791SubstrateBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei243 – 2431SubstrateSequence Analysis
    Binding sitei339 – 3391SubstrateBy similarity
    Active sitei369 – 3691NucleophileBy similarity
    Active sitei386 – 3861Sequence Analysis

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB

    GO - Biological processi

    1. ganglioside catabolic process Source: UniProtKB
    2. oligosaccharide catabolic process Source: Ensembl

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_198596. Glycosphingolipid metabolism.
    REACT_213723. Sialic acid metabolism.

    Protein family/group databases

    CAZyiGH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase-3 (EC:3.2.1.18)
    Alternative name(s):
    Ganglioside sialidase
    Membrane sialidase
    N-acetyl-alpha-neuraminidase 3
    Gene namesi
    Name:Neu3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi619881. Neu3.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 418418Sialidase-3PRO_0000208905Add
    BLAST

    Proteomic databases

    PaxDbiQ99PW5.
    PRIDEiQ99PW5.

    PTM databases

    PhosphoSiteiQ99PW5.

    Expressioni

    Tissue specificityi

    Expressed in brain, cardiac muscle and weakly in liver.

    Gene expression databases

    GenevestigatoriQ99PW5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99PW5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati129 – 14012BNR 1Add
    BLAST
    Repeati201 – 21212BNR 2Add
    BLAST
    Repeati252 – 26312BNR 3Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi24 – 274FRIP motif

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 3 BNR repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG4409.
    GeneTreeiENSGT00390000011171.
    HOGENOMiHOG000233778.
    HOVERGENiHBG052608.
    InParanoidiQ99PW5.
    KOiK12357.
    OMAiVYLFFIC.
    OrthoDBiEOG7MSMNP.
    PhylomeDBiQ99PW5.
    TreeFamiTF331063.

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR026944. Sialidase-3.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF7. PTHR10628:SF7. 1 hit.
    SUPFAMiSSF50939. SSF50939. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q99PW5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEVSSCSLR STLFQQEEQN RITYRIPALL YIPPTHTFLA FAEMRTSSRD    50
    EDAVYLVFRR GVMKGCSVEW GPQQPLMEAT LPGHRTMSPC PVWEKNTGRV 100
    YLFFICVQGH VSERWQLLWG RNAARLCFLY SEDSGCSWGE VKDLTEEVVG 150
    SEMKHWATFA VGPGHGIQLQ SGRLLIPAYA YLISCWFLCF PCSVKPHSLM 200
    FYSDDLGVTW HCGKFIKPQV TGECQVAEVP GKAGNLVLYC SARTPNKFRA 250
    EAFSTDSGDC FQKPTLNQQL CEPRGGCQGS VVSTRPLKMP YTCQDSSGKD 300
    VPSTQKCPLM DRSLEVEEGA GAPSGTWLLY SHPTNKKKRM NLGIYYNQNP 350
    LEVNYWSRPW ILNRGPSGYS DLAVVEGQGL FACLFECGER HEDEKIDFCL 400
    FSDQEVLSCD DCTSPSSN 418
    Length:418
    Mass (Da):46,980
    Last modified:June 1, 2001 - v1
    Checksum:i7CC46F2E5952E240
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026841 mRNA. Translation: BAB32440.1.
    BC100625 mRNA. Translation: AAI00626.1.
    PIRiJC7588.
    RefSeqiNP_446462.1. NM_054010.1.
    UniGeneiRn.67067.

    Genome annotation databases

    EnsembliENSRNOT00000024420; ENSRNOP00000024420; ENSRNOG00000018106.
    GeneIDi117185.
    KEGGirno:117185.
    UCSCiRGD:619881. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026841 mRNA. Translation: BAB32440.1 .
    BC100625 mRNA. Translation: AAI00626.1 .
    PIRi JC7588.
    RefSeqi NP_446462.1. NM_054010.1.
    UniGenei Rn.67067.

    3D structure databases

    ProteinModelPortali Q99PW5.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH33. Glycoside Hydrolase Family 33.

    PTM databases

    PhosphoSitei Q99PW5.

    Proteomic databases

    PaxDbi Q99PW5.
    PRIDEi Q99PW5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024420 ; ENSRNOP00000024420 ; ENSRNOG00000018106 .
    GeneIDi 117185.
    KEGGi rno:117185.
    UCSCi RGD:619881. rat.

    Organism-specific databases

    CTDi 10825.
    RGDi 619881. Neu3.

    Phylogenomic databases

    eggNOGi COG4409.
    GeneTreei ENSGT00390000011171.
    HOGENOMi HOG000233778.
    HOVERGENi HBG052608.
    InParanoidi Q99PW5.
    KOi K12357.
    OMAi VYLFFIC.
    OrthoDBi EOG7MSMNP.
    PhylomeDBi Q99PW5.
    TreeFami TF331063.

    Enzyme and pathway databases

    Reactomei REACT_198596. Glycosphingolipid metabolism.
    REACT_213723. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 620050.
    PROi Q99PW5.

    Gene expression databases

    Genevestigatori Q99PW5.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR026944. Sialidase-3.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF7. PTHR10628:SF7. 1 hit.
    SUPFAMi SSF50939. SSF50939. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of three sialidase genes in rat development."
      Hasegawa T., Feijoo Carnero C., Wada T., Itoyama Y., Miyagi T.
      Biochem. Biophys. Res. Commun. 280:726-732(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.

    Entry informationi

    Entry nameiNEUR3_RAT
    AccessioniPrimary (citable) accession number: Q99PW5
    Secondary accession number(s): Q497C0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3