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Protein

Sialidase-3

Gene

Neu3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25SubstrateBy similarity1
Binding sitei45SubstrateBy similarity1
Active sitei50Proton acceptorBy similarity1
Binding sitei179SubstrateBy similarity1
Binding sitei181SubstrateBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei243SubstrateSequence analysis1
Binding sitei339SubstrateBy similarity1
Active sitei369NucleophileBy similarity1
Active sitei386Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-1660662. Glycosphingolipid metabolism.
R-RNO-4085001. Sialic acid metabolism.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-3 (EC:3.2.1.18)
Alternative name(s):
Ganglioside sialidase
Membrane sialidase
N-acetyl-alpha-neuraminidase 3
Gene namesi
Name:Neu3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi619881. Neu3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002089051 – 418Sialidase-3Add BLAST418

Proteomic databases

PaxDbiQ99PW5.
PRIDEiQ99PW5.

PTM databases

PhosphoSitePlusiQ99PW5.

Expressioni

Tissue specificityi

Expressed in brain, cardiac muscle and weakly in liver.

Gene expression databases

BgeeiENSRNOG00000018106.
GenevisibleiQ99PW5. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024420.

Structurei

3D structure databases

ProteinModelPortaliQ99PW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati129 – 140BNR 1Add BLAST12
Repeati201 – 212BNR 2Add BLAST12
Repeati252 – 263BNR 3Add BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi24 – 27FRIP motif4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 3 BNR repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFVF. Eukaryota.
ENOG410Y74Z. LUCA.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ99PW5.
KOiK12357.
OMAiVYLFFIC.
OrthoDBiEOG091G08BI.
PhylomeDBiQ99PW5.
TreeFamiTF331063.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF23. PTHR10628:SF23. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Q99PW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEVSSCSLR STLFQQEEQN RITYRIPALL YIPPTHTFLA FAEMRTSSRD
60 70 80 90 100
EDAVYLVFRR GVMKGCSVEW GPQQPLMEAT LPGHRTMSPC PVWEKNTGRV
110 120 130 140 150
YLFFICVQGH VSERWQLLWG RNAARLCFLY SEDSGCSWGE VKDLTEEVVG
160 170 180 190 200
SEMKHWATFA VGPGHGIQLQ SGRLLIPAYA YLISCWFLCF PCSVKPHSLM
210 220 230 240 250
FYSDDLGVTW HCGKFIKPQV TGECQVAEVP GKAGNLVLYC SARTPNKFRA
260 270 280 290 300
EAFSTDSGDC FQKPTLNQQL CEPRGGCQGS VVSTRPLKMP YTCQDSSGKD
310 320 330 340 350
VPSTQKCPLM DRSLEVEEGA GAPSGTWLLY SHPTNKKKRM NLGIYYNQNP
360 370 380 390 400
LEVNYWSRPW ILNRGPSGYS DLAVVEGQGL FACLFECGER HEDEKIDFCL
410
FSDQEVLSCD DCTSPSSN
Length:418
Mass (Da):46,980
Last modified:June 1, 2001 - v1
Checksum:i7CC46F2E5952E240
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026841 mRNA. Translation: BAB32440.1.
BC100625 mRNA. Translation: AAI00626.1.
PIRiJC7588.
RefSeqiNP_446462.1. NM_054010.1.
XP_008757887.1. XM_008759665.2.
UniGeneiRn.67067.

Genome annotation databases

EnsembliENSRNOT00000024420; ENSRNOP00000024420; ENSRNOG00000018106.
GeneIDi117185.
KEGGirno:117185.
UCSCiRGD:619881. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026841 mRNA. Translation: BAB32440.1.
BC100625 mRNA. Translation: AAI00626.1.
PIRiJC7588.
RefSeqiNP_446462.1. NM_054010.1.
XP_008757887.1. XM_008759665.2.
UniGeneiRn.67067.

3D structure databases

ProteinModelPortaliQ99PW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024420.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

PTM databases

PhosphoSitePlusiQ99PW5.

Proteomic databases

PaxDbiQ99PW5.
PRIDEiQ99PW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024420; ENSRNOP00000024420; ENSRNOG00000018106.
GeneIDi117185.
KEGGirno:117185.
UCSCiRGD:619881. rat.

Organism-specific databases

CTDi10825.
RGDi619881. Neu3.

Phylogenomic databases

eggNOGiENOG410IFVF. Eukaryota.
ENOG410Y74Z. LUCA.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ99PW5.
KOiK12357.
OMAiVYLFFIC.
OrthoDBiEOG091G08BI.
PhylomeDBiQ99PW5.
TreeFamiTF331063.

Enzyme and pathway databases

ReactomeiR-RNO-1660662. Glycosphingolipid metabolism.
R-RNO-4085001. Sialic acid metabolism.

Miscellaneous databases

PROiQ99PW5.

Gene expression databases

BgeeiENSRNOG00000018106.
GenevisibleiQ99PW5. RN.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF23. PTHR10628:SF23. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNEUR3_RAT
AccessioniPrimary (citable) accession number: Q99PW5
Secondary accession number(s): Q497C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.