Reviewed, UniProtKB/Swiss-Prot Q99PW5 (NEUR3_RAT)

Names and origin

Protein names

Recommended name:
    Sialidase-3
    EC=3.2.1.18
Alternative name(s):
    Membrane sialidase
    Ganglioside sialidase
    N-acetyl-alpha-neuraminidase 3

Gene names

Name:

Neu3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in brain, cardiac muscle and weakly in liver.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 3 BNR repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat
Molecular function	Glycosidase Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Sialidase-3		PRO_0000208905
Regions
	Repeat	129 – 140	12	BNR 1
	Repeat	201 – 212	12	BNR 2
	Repeat	252 – 263	12	BNR 3
	Motif	24 – 27	4	FRIP motif
Sites
	Active site	50	1	Proton acceptor By similarity
	Active site	369	1	Nucleophile By similarity
	Active site	386	1	Potential
	Binding site	25	1	Substrate By similarity
	Binding site	243	1	Substrate Potential
	Binding site	339	1	Substrate By similarity
Amino acid modifications
	Modified residue	414	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99PW5-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7CC46F2E5952E240	FASTA	418	46,980
	10 20 30 40 50 60 MEEVSSCSLR STLFQQEEQN RITYRIPALL YIPPTHTFLA FAEMRTSSRD EDAVYLVFRR 70 80 90 100 110 120 GVMKGCSVEW GPQQPLMEAT LPGHRTMSPC PVWEKNTGRV YLFFICVQGH VSERWQLLWG 130 140 150 160 170 180 RNAARLCFLY SEDSGCSWGE VKDLTEEVVG SEMKHWATFA VGPGHGIQLQ SGRLLIPAYA 190 200 210 220 230 240 YLISCWFLCF PCSVKPHSLM FYSDDLGVTW HCGKFIKPQV TGECQVAEVP GKAGNLVLYC 250 260 270 280 290 300 SARTPNKFRA EAFSTDSGDC FQKPTLNQQL CEPRGGCQGS VVSTRPLKMP YTCQDSSGKD 310 320 330 340 350 360 VPSTQKCPLM DRSLEVEEGA GAPSGTWLLY SHPTNKKKRM NLGIYYNQNP LEVNYWSRPW 370 380 390 400 410 ILNRGPSGYS DLAVVEGQGL FACLFECGER HEDEKIDFCL FSDQEVLSCD DCTSPSSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential expression of three sialidase genes in rat development." Hasegawa T., Feijoo Carnero C., Wada T., Itoyama Y., Miyagi T. Biochem. Biophys. Res. Commun. 280:726-732(2001) [PubMed: 11162581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026841 mRNA. Translation: BAB32440.1. BC100625 mRNA. Translation: AAI00626.1.
IPI	IPI00201459.
PIR	JC7588.
RefSeq	NP_446462.1.
UniGene	Rn.67067
3D structure databases
SMR	Q99PW5. Positions 10-408.
ModBase	Search...
Protein-protein interaction databases
STRING	Q99PW5.
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
PTM databases
PhosphoSite	Q99PW5.
Genome annotation databases
Ensembl	ENSRNOT00000024420; ENSRNOP00000024420; ENSRNOG00000018106; Rattus norvegicus. [Genome view]
GeneID	117185.
KEGG	rno:117185.
NMPDR	fig|10116.3.peg.2396.
UCSC	NM_054010. rat.
Organism-specific databases
CTD	117185.
RGD	619881. Neu3.
Phylogenomic databases
eggNOG	roNOG15095.
HOVERGEN	Q99PW5.
InParanoid	Q99PW5.
OMA	FICVRGH.
OrthoDB	EOG96DPNJ.
PhylomeDB	Q99PW5.
Enzyme and pathway databases
BRENDA	3.2.1.18. 248.
Gene expression databases
ArrayExpress	Q99PW5.
Genevestigator	Q99PW5.
GermOnline	ENSRNOG00000018106. Rattus norvegicus.
Family and domain databases
InterPro	IPR011040. Neuraminidase. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620050.

Entry information

Entry name

NEUR3_RAT

Accession

Primary (citable) accession number: Q99PW5
Secondary accession number(s): Q497C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	June 1, 2001
Last modified:	January 19, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)