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Protein

Sialidase-3

Gene

Neu3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251SubstrateBy similarity
Binding sitei45 – 451SubstrateBy similarity
Active sitei50 – 501Proton acceptorBy similarity
Binding sitei179 – 1791SubstrateBy similarity
Binding sitei181 – 1811SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei243 – 2431SubstrateSequence Analysis
Binding sitei339 – 3391SubstrateBy similarity
Active sitei369 – 3691NucleophileBy similarity
Active sitei386 – 3861Sequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_283933. Sialic acid metabolism.
REACT_330669. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-3 (EC:3.2.1.18)
Alternative name(s):
Ganglioside sialidase
Membrane sialidase
N-acetyl-alpha-neuraminidase 3
Gene namesi
Name:Neu3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi619881. Neu3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Sialidase-3PRO_0000208905Add
BLAST

Proteomic databases

PaxDbiQ99PW5.
PRIDEiQ99PW5.

PTM databases

PhosphoSiteiQ99PW5.

Expressioni

Tissue specificityi

Expressed in brain, cardiac muscle and weakly in liver.

Gene expression databases

GenevestigatoriQ99PW5.

Structurei

3D structure databases

ProteinModelPortaliQ99PW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati129 – 14012BNR 1Add
BLAST
Repeati201 – 21212BNR 2Add
BLAST
Repeati252 – 26312BNR 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi24 – 274FRIP motif

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 3 BNR repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG4409.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ99PW5.
KOiK12357.
OMAiVYLFFIC.
OrthoDBiEOG7MSMNP.
PhylomeDBiQ99PW5.
TreeFamiTF331063.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF7. PTHR10628:SF7. 1 hit.
SUPFAMiSSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Q99PW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEVSSCSLR STLFQQEEQN RITYRIPALL YIPPTHTFLA FAEMRTSSRD
60 70 80 90 100
EDAVYLVFRR GVMKGCSVEW GPQQPLMEAT LPGHRTMSPC PVWEKNTGRV
110 120 130 140 150
YLFFICVQGH VSERWQLLWG RNAARLCFLY SEDSGCSWGE VKDLTEEVVG
160 170 180 190 200
SEMKHWATFA VGPGHGIQLQ SGRLLIPAYA YLISCWFLCF PCSVKPHSLM
210 220 230 240 250
FYSDDLGVTW HCGKFIKPQV TGECQVAEVP GKAGNLVLYC SARTPNKFRA
260 270 280 290 300
EAFSTDSGDC FQKPTLNQQL CEPRGGCQGS VVSTRPLKMP YTCQDSSGKD
310 320 330 340 350
VPSTQKCPLM DRSLEVEEGA GAPSGTWLLY SHPTNKKKRM NLGIYYNQNP
360 370 380 390 400
LEVNYWSRPW ILNRGPSGYS DLAVVEGQGL FACLFECGER HEDEKIDFCL
410
FSDQEVLSCD DCTSPSSN
Length:418
Mass (Da):46,980
Last modified:June 1, 2001 - v1
Checksum:i7CC46F2E5952E240
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026841 mRNA. Translation: BAB32440.1.
BC100625 mRNA. Translation: AAI00626.1.
PIRiJC7588.
RefSeqiNP_446462.1. NM_054010.1.
XP_008757887.1. XM_008759665.1.
UniGeneiRn.67067.

Genome annotation databases

EnsembliENSRNOT00000024420; ENSRNOP00000024420; ENSRNOG00000018106.
GeneIDi117185.
KEGGirno:117185.
UCSCiRGD:619881. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026841 mRNA. Translation: BAB32440.1.
BC100625 mRNA. Translation: AAI00626.1.
PIRiJC7588.
RefSeqiNP_446462.1. NM_054010.1.
XP_008757887.1. XM_008759665.1.
UniGeneiRn.67067.

3D structure databases

ProteinModelPortaliQ99PW5.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

PTM databases

PhosphoSiteiQ99PW5.

Proteomic databases

PaxDbiQ99PW5.
PRIDEiQ99PW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024420; ENSRNOP00000024420; ENSRNOG00000018106.
GeneIDi117185.
KEGGirno:117185.
UCSCiRGD:619881. rat.

Organism-specific databases

CTDi10825.
RGDi619881. Neu3.

Phylogenomic databases

eggNOGiCOG4409.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ99PW5.
KOiK12357.
OMAiVYLFFIC.
OrthoDBiEOG7MSMNP.
PhylomeDBiQ99PW5.
TreeFamiTF331063.

Enzyme and pathway databases

ReactomeiREACT_283933. Sialic acid metabolism.
REACT_330669. Glycosphingolipid metabolism.

Miscellaneous databases

NextBioi620050.
PROiQ99PW5.

Gene expression databases

GenevestigatoriQ99PW5.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF7. PTHR10628:SF7. 1 hit.
SUPFAMiSSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression of three sialidase genes in rat development."
    Hasegawa T., Feijoo Carnero C., Wada T., Itoyama Y., Miyagi T.
    Biochem. Biophys. Res. Commun. 280:726-732(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiNEUR3_RAT
AccessioniPrimary (citable) accession number: Q99PW5
Secondary accession number(s): Q497C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: April 1, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.