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Q99PW5 (NEUR3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase-3

EC=3.2.1.18
Alternative name(s):
Ganglioside sialidase
Membrane sialidase
N-acetyl-alpha-neuraminidase 3
Gene names
Name:Neu3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates By similarity.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed in brain, cardiac muscle and weakly in liver.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 3 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Sialidase-3
PRO_0000208905

Regions

Repeat129 – 14012BNR 1
Repeat201 – 21212BNR 2
Repeat252 – 26312BNR 3
Motif24 – 274FRIP motif

Sites

Active site501Proton acceptor By similarity
Active site3691Nucleophile By similarity
Active site3861 Potential
Binding site251Substrate By similarity
Binding site451Substrate By similarity
Binding site1791Substrate By similarity
Binding site1811Substrate By similarity
Binding site2231Substrate By similarity
Binding site2431Substrate Potential
Binding site3391Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99PW5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 7CC46F2E5952E240

FASTA41846,980
        10         20         30         40         50         60 
MEEVSSCSLR STLFQQEEQN RITYRIPALL YIPPTHTFLA FAEMRTSSRD EDAVYLVFRR 

        70         80         90        100        110        120 
GVMKGCSVEW GPQQPLMEAT LPGHRTMSPC PVWEKNTGRV YLFFICVQGH VSERWQLLWG 

       130        140        150        160        170        180 
RNAARLCFLY SEDSGCSWGE VKDLTEEVVG SEMKHWATFA VGPGHGIQLQ SGRLLIPAYA 

       190        200        210        220        230        240 
YLISCWFLCF PCSVKPHSLM FYSDDLGVTW HCGKFIKPQV TGECQVAEVP GKAGNLVLYC 

       250        260        270        280        290        300 
SARTPNKFRA EAFSTDSGDC FQKPTLNQQL CEPRGGCQGS VVSTRPLKMP YTCQDSSGKD 

       310        320        330        340        350        360 
VPSTQKCPLM DRSLEVEEGA GAPSGTWLLY SHPTNKKKRM NLGIYYNQNP LEVNYWSRPW 

       370        380        390        400        410 
ILNRGPSGYS DLAVVEGQGL FACLFECGER HEDEKIDFCL FSDQEVLSCD DCTSPSSN 

« Hide

References

« Hide 'large scale' references
[1]"Differential expression of three sialidase genes in rat development."
Hasegawa T., Feijoo Carnero C., Wada T., Itoyama Y., Miyagi T.
Biochem. Biophys. Res. Commun. 280:726-732(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026841 mRNA. Translation: BAB32440.1.
BC100625 mRNA. Translation: AAI00626.1.
PIRJC7588.
RefSeqNP_446462.1. NM_054010.1.
UniGeneRn.67067.

3D structure databases

ProteinModelPortalQ99PW5.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

PTM databases

PhosphoSiteQ99PW5.

Proteomic databases

PaxDbQ99PW5.
PRIDEQ99PW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000024420; ENSRNOP00000024420; ENSRNOG00000018106.
GeneID117185.
KEGGrno:117185.
UCSCRGD:619881. rat.

Organism-specific databases

CTD10825.
RGD619881. Neu3.

Phylogenomic databases

eggNOGCOG4409.
GeneTreeENSGT00390000011171.
HOGENOMHOG000233778.
HOVERGENHBG052608.
InParanoidQ99PW5.
KOK12357.
OMAVYLFFIC.
OrthoDBEOG7MSMNP.
PhylomeDBQ99PW5.
TreeFamTF331063.

Gene expression databases

GenevestigatorQ99PW5.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF7. PTHR10628:SF7. 1 hit.
SUPFAMSSF50939. SSF50939. 2 hits.
ProtoNetSearch...

Other

NextBio620050.
PROQ99PW5.

Entry information

Entry nameNEUR3_RAT
AccessionPrimary (citable) accession number: Q99PW5
Secondary accession number(s): Q497C0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries