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Protein

Sialidase-1

Gene

Neu1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.1 Publication

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Binding sitei91SubstrateBy similarity1
Active sitei97Proton acceptorBy similarity1
Active sitei258Sequence analysis1
Binding sitei258SubstrateBy similarity1
Binding sitei274SubstrateBy similarity1
Binding sitei335SubstrateBy similarity1
Active sitei364NucleophileBy similarity1
Active sitei388Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • ganglioside catabolic process Source: GO_Central
  • oligosaccharide catabolic process Source: UniProtKB
  • positive regulation of neuron projection development Source: RGD
  • regulation of myoblast proliferation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-1 (EC:3.2.1.18)
Alternative name(s):
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1
Gene namesi
Name:Neu1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3163. Neu1.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: RGD
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • lysosomal lumen Source: UniProtKB-SubCell
  • lysosomal membrane Source: UniProtKB-SubCell
  • lysosome Source: RGD
  • membrane Source: GO_Central
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 39Sequence analysisAdd BLAST39
ChainiPRO_000001202840 – 409Sialidase-1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi180N-linked (GlcNAc...)Sequence analysis1
Glycosylationi337N-linked (GlcNAc...)Sequence analysis1
Glycosylationi346N-linked (GlcNAc...)Sequence analysis1
Glycosylationi372N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.Curated
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ99PW3.

Interactioni

Subunit structurei

Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.By similarity

Protein-protein interaction databases

IntActiQ99PW3. 1 interactor.
STRINGi10116.ENSRNOP00000050614.

Structurei

3D structure databases

ProteinModelPortaliQ99PW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati106 – 117BNR 1Add BLAST12
Repeati166 – 177BNR 2Add BLAST12
Repeati225 – 236BNR 3Add BLAST12
Repeati341 – 352BNR 4Add BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi71 – 74FRIP motif4
Motifi406 – 409Internalization signal4

Domaini

A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IE33. Eukaryota.
COG4409. LUCA.
HOVERGENiHBG057314.
InParanoidiQ99PW3.
PhylomeDBiQ99PW3.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGAEPSRPP GPPSYWTGRR GQGLAAIFLL LVSAAGSEAR TEDDFSLVQP
60 70 80 90 100
LVTMEQLLWV SGKQIGSVDT FRIPLITATP RGTLLAFAEA RKKSASDEGA
110 120 130 140 150
KFIAMRRSTD QGSTWSSTAF IVDDGEASDG LNLGAVVNDV DTGVVFLIYT
160 170 180 190 200
LCAHKVNCQV ASTMLVWSKD DGVSWSPPRN LSVDIGTEMF APGPGSGIQK
210 220 230 240 250
QREPWKGRLI VCGHGTLERD GVFCLLSDDH GASWHYGTGV SGIPFGQPKH
260 270 280 290 300
DHDFNPDECQ PYELPDGSVI INARNQNNYH CRCRIVLRSY DACDTLRPRD
310 320 330 340 350
VTFDPELVDP VVAAGALATS SGIVFFSNPA HPEYRVNLTL RWSFSNGTFW
360 370 380 390 400
QKERVQLWPG PSGYSSLTAL ENSTDGKKQP PQLFVLYEKG LNRYTESISM

VKISVYGTL
Length:409
Mass (Da):44,695
Last modified:June 1, 2001 - v1
Checksum:iF912FA1E1F0991A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035722 mRNA. Translation: BAB21443.1.
UniGeneiRn.128560.

Genome annotation databases

UCSCiRGD:3163. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035722 mRNA. Translation: BAB21443.1.
UniGeneiRn.128560.

3D structure databases

ProteinModelPortaliQ99PW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99PW3. 1 interactor.
STRINGi10116.ENSRNOP00000050614.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PaxDbiQ99PW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3163. rat.

Organism-specific databases

RGDi3163. Neu1.

Phylogenomic databases

eggNOGiENOG410IE33. Eukaryota.
COG4409. LUCA.
HOVERGENiHBG057314.
InParanoidiQ99PW3.
PhylomeDBiQ99PW3.

Miscellaneous databases

PROiQ99PW3.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNEUR1_RAT
AccessioniPrimary (citable) accession number: Q99PW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.