Q99PW3 (NEUR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialidase-1
EC=3.2.1.18

Alternative name(s):
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1

Gene names

Name:

Neu1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subunit structure	Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.
Subcellular location	Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
Domain	A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.
Post-translational modification	N-glycosylated Probable. Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 4 BNR repeats.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasmic vesicle Lysosome Membrane
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal lumen Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Potential
	Chain	40 – 409	370	Sialidase-1		PRO_0000012028
Regions
	Repeat	106 – 117	12	BNR 1
	Repeat	166 – 177	12	BNR 2
	Repeat	225 – 236	12	BNR 3
	Repeat	341 – 352	12	BNR 4
	Motif	71 – 74	4	FRIP motif
	Motif	406 – 409	4	Internalization signal
Sites
	Active site	97	1	Proton acceptor By similarity
	Active site	258	1	Potential
	Active site	364	1	Nucleophile By similarity
	Binding site	72	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity
	Binding site	335	1	Substrate By similarity
Amino acid modifications
	Modified residue	236	1	Phosphotyrosine By similarity
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	372	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q99PW3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F912FA1E1F0991A9	FASTA	409	44,695
	10 20 30 40 50 60 MVGAEPSRPP GPPSYWTGRR GQGLAAIFLL LVSAAGSEAR TEDDFSLVQP LVTMEQLLWV 70 80 90 100 110 120 SGKQIGSVDT FRIPLITATP RGTLLAFAEA RKKSASDEGA KFIAMRRSTD QGSTWSSTAF 130 140 150 160 170 180 IVDDGEASDG LNLGAVVNDV DTGVVFLIYT LCAHKVNCQV ASTMLVWSKD DGVSWSPPRN 190 200 210 220 230 240 LSVDIGTEMF APGPGSGIQK QREPWKGRLI VCGHGTLERD GVFCLLSDDH GASWHYGTGV 250 260 270 280 290 300 SGIPFGQPKH DHDFNPDECQ PYELPDGSVI INARNQNNYH CRCRIVLRSY DACDTLRPRD 310 320 330 340 350 360 VTFDPELVDP VVAAGALATS SGIVFFSNPA HPEYRVNLTL RWSFSNGTFW QKERVQLWPG 370 380 390 400 PSGYSSLTAL ENSTDGKKQP PQLFVLYEKG LNRYTESISM VKISVYGTL

References

[1]	"Differential expression of three sialidase genes in rat development." Hasegawa T., Feijoo Carnero C., Wada T., Itoyama Y., Miyagi T. Biochem. Biophys. Res. Commun. 280:726-732(2001) [PubMed: 11162581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB035722 mRNA. Translation: BAB21443.1.
IPI	IPI00201456.
UniGene	Rn.128560.
3D structure databases
ProteinModelPortal	Q99PW3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99PW3. 1 interaction.
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	3163. Neu1.
Phylogenomic databases
HOVERGEN	HBG057314.
Gene expression databases
Genevestigator	Q99PW3.
Family and domain databases
InterPro	IPR011040. Neuraminidase. [Graphical view]
Gene3D	G3DSA:2.120.10.10. Neuraminidase. 1 hit.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NEUR1_RAT

Accession

Primary (citable) accession number: Q99PW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program