Q99PV5 (BHE41_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Class E basic helix-loop-helix protein 41
Short name=bHLHe41

Alternative name(s):
Class B basic helix-loop-helix protein 3
Short name=bHLHb3
Differentially expressed in chondrocytes protein 2
Short name=mDEC2

Gene names

Name:	Bhlhe41
Synonyms:	Bhlhb3, Dec2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be a transcriptional repressor that represses both basal and activated transcription.
Subunit structure	Homodimerize.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in skeletal muscle, brain and lung.
Miscellaneous	Activity profiles and sleep recordings of transgenic mice carrying the mutation Arg-325 show increased vigilance time and less sleep time than control mice in a zeitgeber time- and sleep deprivation-dependent manner.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	circadian regulation of gene expression Inferred from mutant phenotype PubMed 18411297. Source: BHF-UCL negative regulation of myotube differentiation Inferred from direct assay PubMed 12657651. Source: BHF-UCL negative regulation of transcription by competitive promoter binding Inferred from direct assay PubMed 12657651. Source: BHF-UCL
Cellular_component	transcriptional repressor complex Traceable author statement PubMed 12175508. Source: MGI
Molecular_function	E-box binding Inferred from direct assay PubMed 12397359PubMed 12657651PubMed 15147242PubMed 17487425. Source: BHF-UCL MRF binding Inferred from direct assay PubMed 12657651PubMed 17487425. Source: BHF-UCL RNA polymerase II activating transcription factor binding Inferred from direct assay PubMed 17487425. Source: BHF-UCL RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay PubMed 12657651. Source: BHF-UCL RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay PubMed 12657651PubMed 15147242PubMed 17487425. Source: BHF-UCL RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 15147242. Source: BHF-UCL RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay PubMed 12397359PubMed 12657651. Source: BHF-UCL histone deacetylase binding Inferred from direct assay PubMed 17487425. Source: BHF-UCL protein heterodimerization activity Inferred from direct assay PubMed 17487425. Source: BHF-UCL transcription corepressor activity Traceable author statement PubMed 12175508. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Class E basic helix-loop-helix protein 41		PRO_0000127148
Regions
	Domain	44 – 99	56	bHLH
	Domain	131 – 166	36	Orange
	Compositional bias	321 – 373	53	Ala/Gly-rich

Sequences

	Sequence		Length	Mass (Da)
	Q99PV5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 40A87281B08E233D	FASTA	410	43,946
	10 20 30 40 50 60 MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR LIEKKRRDRI 70 80 90 100 110 120 NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT ALTEQQHQKI IALQNGERSL 130 140 150 160 170 180 KSPVQADLDA FHSGFQTCAK EVLQYLARFE SWTPREPRCA QLVSHLHAVA TQLLTPQVPS 190 200 210 220 230 240 GRGSGRAPCS AGAAAASGPE RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK 250 260 270 280 290 300 QEPPGDSSPA PKRPKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS 310 320 330 340 350 360 AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS VLSPPPEKAG 370 380 390 400 410 ATAGAPFLAH EVAPPGPLRP QHAHSRTHLP RAVNPESSQE DATQPAKDAP

References

[1]	"Molecular cloning and characterization of DEC2, a new member of basic helix-loop-helix proteins." Fujimoto K., Shen M., Noshiro M., Matsubara K., Shingu S., Honda K., Yoshida E., Suardita K., Matsuda Y., Kato Y. Biochem. Biophys. Res. Commun. 280:164-171(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional repressor DEC2 regulates sleep length in mammals." He Y., Jones C.R., Fujiki N., Xu Y., Guo B., Holder J.L. Jr., Rossner M.J., Nishino S., Fu Y.H. Science 325:866-870(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TRANSGENIC MICE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB044090 mRNA. Translation: BAB21503.1.
IPI	IPI00121602.
PIR	JC7584.
RefSeq	NP_001258697.1. NM_001271768.1. NP_077789.1. NM_024469.2.
UniGene	Mm.154529.
3D structure databases
ProteinModelPortal	Q99PV5.
SMR	Q99PV5. Positions 49-101.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-57696N.
IntAct	Q99PV5. 19 interactions.
MINT	MINT-7012655.
STRING	10090.ENSMUSP00000032386.
PTM databases
PhosphoSite	Q99PV5.
Proteomic databases
PRIDE	Q99PV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256.
GeneID	79362.
KEGG	mmu:79362.
Organism-specific databases
CTD	79365.
MGI	MGI:1930704. Bhlhe41.
Phylogenomic databases
eggNOG	NOG235394.
GeneTree	ENSGT00510000046898.
HOGENOM	HOG000234381.
InParanoid	Q99PV5.
KO	K03730.
OMA	AYKLPHR.
OrthoDB	EOG4V6ZHP.
Enzyme and pathway databases
Reactome	REACT_109335. Circadian Clock. REACT_24972. Circadian Clock (mouse).
Gene expression databases
ArrayExpress	Q99PV5.
Bgee	Q99PV5.
Genevestigator	Q99PV5.
GermOnline	ENSMUSG00000030256. Mus musculus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. IPR003650. Orange. IPR018352. Orange_subgr. [Graphical view]
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00511. ORANGE. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BHLHE41. mouse.
NextBio	349911.
SOURCE	Search...

Entry information

Entry name

BHE41_MOUSE

Accession

Primary (citable) accession number: Q99PV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program