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Protein

Class E basic helix-loop-helix protein 41

Gene

Bhlhe41

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.5 Publications

Miscellaneous

Activity profiles and sleep recordings of transgenic mice carrying the mutation Arg-325 show increased vigilance time and less sleep time than control mice in a zeitgeber time- and sleep deprivation-dependent manner.

GO - Molecular functioni

  • bHLH transcription factor binding Source: BHF-UCL
  • E-box binding Source: BHF-UCL
  • histone deacetylase binding Source: BHF-UCL
  • MRF binding Source: BHF-UCL
  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • RNA polymerase II activating transcription factor binding Source: BHF-UCL
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II transcription factor binding Source: BHF-UCL
  • transcription corepressor activity Source: UniProtKB
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: BHF-UCL

GO - Biological processi

  • circadian regulation of gene expression Source: BHF-UCL
  • circadian rhythm Source: UniProtKB
  • negative regulation of myotube differentiation Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by competitive promoter binding Source: BHF-UCL
  • negative regulation of transcription by RNA polymerase II Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1368110 Bmal1:Clock,Npas2 activates circadian gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Class E basic helix-loop-helix protein 41
Short name:
bHLHe41
Alternative name(s):
Class B basic helix-loop-helix protein 3
Short name:
bHLHb3
Differentially expressed in chondrocytes protein 2
Short name:
mDEC2
Gene namesi
Name:Bhlhe41
Synonyms:Bhlhb3, Dec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1930704 Bhlhe41

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271481 – 410Class E basic helix-loop-helix protein 41Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki31Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki240Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ99PV5
PaxDbiQ99PV5
PRIDEiQ99PV5

PTM databases

iPTMnetiQ99PV5
PhosphoSitePlusiQ99PV5

Expressioni

Tissue specificityi

Expressed in skeletal muscle, brain and lung.

Inductioni

Expressed in a circdadian manner in the liver with a peak at ZT10.1 Publication

Gene expression databases

BgeeiENSMUSG00000030256
ExpressionAtlasiQ99PV5 baseline and differential
GenevisibleiQ99PV5 MM

Interactioni

Subunit structurei

Homodimer. Interacts with CIART. Interacts with ARNTL/BMAL1 and RXRA.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • bHLH transcription factor binding Source: BHF-UCL
  • histone deacetylase binding Source: BHF-UCL
  • MRF binding Source: BHF-UCL
  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • RNA polymerase II activating transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi2197553 interactors.
DIPiDIP-57696N
IntActiQ99PV5 25 interactors.
MINTiQ99PV5
STRINGi10090.ENSMUSP00000032386

Structurei

3D structure databases

ProteinModelPortaliQ99PV5
SMRiQ99PV5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 99bHLHPROSITE-ProRule annotationAdd BLAST56
Domaini131 – 166OrangePROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi321 – 373Ala/Gly-richAdd BLAST53

Phylogenomic databases

eggNOGiKOG4304 Eukaryota
ENOG4111F0X LUCA
GeneTreeiENSGT00510000046898
HOGENOMiHOG000234381
InParanoidiQ99PV5
KOiK03730
OMAiLYMCKSK
OrthoDBiEOG091G0D3I
PhylomeDBiQ99PV5
TreeFamiTF330859

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.101 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003650 Orange_dom
PfamiView protein in Pfam
PF07527 Hairy_orange, 1 hit
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00511 ORANGE, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS51054 ORANGE, 1 hit

Sequencei

Sequence statusi: Complete.

Q99PV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR
60 70 80 90 100
LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT
110 120 130 140 150
ALTEQQHQKI IALQNGERSL KSPVQADLDA FHSGFQTCAK EVLQYLARFE
160 170 180 190 200
SWTPREPRCA QLVSHLHAVA TQLLTPQVPS GRGSGRAPCS AGAAAASGPE
210 220 230 240 250
RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK QEPPGDSSPA
260 270 280 290 300
PKRPKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS
310 320 330 340 350
AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS
360 370 380 390 400
VLSPPPEKAG ATAGAPFLAH EVAPPGPLRP QHAHSRTHLP RAVNPESSQE
410
DATQPAKDAP
Length:410
Mass (Da):43,946
Last modified:June 1, 2001 - v1
Checksum:i40A87281B08E233D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044090 mRNA Translation: BAB21503.1
CCDSiCCDS20697.1
PIRiJC7584
RefSeqiNP_001258697.1, NM_001271768.1
NP_077789.1, NM_024469.2
UniGeneiMm.154529

Genome annotation databases

EnsembliENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256
GeneIDi79362
KEGGimmu:79362
UCSCiuc009ert.2 mouse

Similar proteinsi

Entry informationi

Entry nameiBHE41_MOUSE
AccessioniPrimary (citable) accession number: Q99PV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome