Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99PV5

- BHE41_MOUSE

UniProt

Q99PV5 - BHE41_MOUSE

Protein

Class E basic helix-loop-helix protein 41

Gene

Bhlhe41

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.5 Publications

    GO - Molecular functioni

    1. bHLH transcription factor binding Source: BHF-UCL
    2. E-box binding Source: BHF-UCL
    3. histone deacetylase binding Source: BHF-UCL
    4. MRF binding Source: BHF-UCL
    5. protein binding Source: UniProtKB
    6. protein heterodimerization activity Source: BHF-UCL
    7. protein homodimerization activity Source: BHF-UCL
    8. RNA polymerase II activating transcription factor binding Source: BHF-UCL
    9. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: BHF-UCL
    10. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
    11. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    12. RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
    13. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: BHF-UCL
    14. transcription corepressor activity Source: UniProtKB

    GO - Biological processi

    1. circadian regulation of gene expression Source: BHF-UCL
    2. circadian rhythm Source: UniProtKB
    3. negative regulation of myotube differentiation Source: BHF-UCL
    4. negative regulation of transcription, DNA-templated Source: UniProtKB
    5. negative regulation of transcription by competitive promoter binding Source: BHF-UCL
    6. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    7. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Biological rhythms, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
    REACT_198620. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class E basic helix-loop-helix protein 41
    Short name:
    bHLHe41
    Alternative name(s):
    Class B basic helix-loop-helix protein 3
    Short name:
    bHLHb3
    Differentially expressed in chondrocytes protein 2
    Short name:
    mDEC2
    Gene namesi
    Name:Bhlhe41
    Synonyms:Bhlhb3, Dec2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1930704. Bhlhe41.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome
    2. transcriptional repressor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Class E basic helix-loop-helix protein 41PRO_0000127148Add
    BLAST

    Proteomic databases

    PRIDEiQ99PV5.

    PTM databases

    PhosphoSiteiQ99PV5.

    Expressioni

    Tissue specificityi

    Expressed in skeletal muscle, brain and lung.

    Inductioni

    Expressed in a circdadian manner in the liver with a peak at ZT10.1 Publication

    Gene expression databases

    ArrayExpressiQ99PV5.
    BgeeiQ99PV5.
    GenevestigatoriQ99PV5.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CIART. Interacts with ARNTL/BMAL1 and RXRA.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CebpbP280335EBI-6143801,EBI-1029979

    Protein-protein interaction databases

    BioGridi219755. 3 interactions.
    DIPiDIP-57696N.
    IntActiQ99PV5. 21 interactions.
    MINTiMINT-7012655.
    STRINGi10090.ENSMUSP00000032386.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99PV5.
    SMRiQ99PV5. Positions 36-100.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 9956bHLHPROSITE-ProRule annotationAdd
    BLAST
    Domaini131 – 16636OrangePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi321 – 37353Ala/Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
    Contains 1 Orange domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG235394.
    GeneTreeiENSGT00510000046898.
    HOGENOMiHOG000234381.
    InParanoidiQ99PV5.
    KOiK03730.
    OMAiAYKLPHR.
    OrthoDBiEOG75XGKZ.
    PhylomeDBiQ99PV5.
    TreeFamiTF330859.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR003650. Orange.
    IPR018352. Orange_subgr.
    [Graphical view]
    PfamiPF07527. Hairy_orange. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    SM00511. ORANGE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    PS51054. ORANGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99PV5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR    50
    LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT 100
    ALTEQQHQKI IALQNGERSL KSPVQADLDA FHSGFQTCAK EVLQYLARFE 150
    SWTPREPRCA QLVSHLHAVA TQLLTPQVPS GRGSGRAPCS AGAAAASGPE 200
    RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK QEPPGDSSPA 250
    PKRPKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS 300
    AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS 350
    VLSPPPEKAG ATAGAPFLAH EVAPPGPLRP QHAHSRTHLP RAVNPESSQE 400
    DATQPAKDAP 410
    Length:410
    Mass (Da):43,946
    Last modified:June 1, 2001 - v1
    Checksum:i40A87281B08E233D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044090 mRNA. Translation: BAB21503.1.
    CCDSiCCDS20697.1.
    PIRiJC7584.
    RefSeqiNP_001258697.1. NM_001271768.1.
    NP_077789.1. NM_024469.2.
    UniGeneiMm.154529.

    Genome annotation databases

    EnsembliENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256.
    GeneIDi79362.
    KEGGimmu:79362.
    UCSCiuc009ert.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044090 mRNA. Translation: BAB21503.1 .
    CCDSi CCDS20697.1.
    PIRi JC7584.
    RefSeqi NP_001258697.1. NM_001271768.1.
    NP_077789.1. NM_024469.2.
    UniGenei Mm.154529.

    3D structure databases

    ProteinModelPortali Q99PV5.
    SMRi Q99PV5. Positions 36-100.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 219755. 3 interactions.
    DIPi DIP-57696N.
    IntActi Q99PV5. 21 interactions.
    MINTi MINT-7012655.
    STRINGi 10090.ENSMUSP00000032386.

    PTM databases

    PhosphoSitei Q99PV5.

    Proteomic databases

    PRIDEi Q99PV5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032386 ; ENSMUSP00000032386 ; ENSMUSG00000030256 .
    GeneIDi 79362.
    KEGGi mmu:79362.
    UCSCi uc009ert.1. mouse.

    Organism-specific databases

    CTDi 79365.
    MGIi MGI:1930704. Bhlhe41.

    Phylogenomic databases

    eggNOGi NOG235394.
    GeneTreei ENSGT00510000046898.
    HOGENOMi HOG000234381.
    InParanoidi Q99PV5.
    KOi K03730.
    OMAi AYKLPHR.
    OrthoDBi EOG75XGKZ.
    PhylomeDBi Q99PV5.
    TreeFami TF330859.

    Enzyme and pathway databases

    Reactomei REACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
    REACT_198620. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

    Miscellaneous databases

    ChiTaRSi BHLHE41. mouse.
    NextBioi 349911.
    PROi Q99PV5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99PV5.
    Bgeei Q99PV5.
    Genevestigatori Q99PV5.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR003650. Orange.
    IPR018352. Orange_subgr.
    [Graphical view ]
    Pfami PF07527. Hairy_orange. 1 hit.
    PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    SM00511. ORANGE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    PS51054. ORANGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of DEC2, a new member of basic helix-loop-helix proteins."
      Fujimoto K., Shen M., Noshiro M., Matsubara K., Shingu S., Honda K., Yoshida E., Suardita K., Matsuda Y., Kato Y.
      Biochem. Biophys. Res. Commun. 280:164-171(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Dec1 and Dec2 are regulators of the mammalian molecular clock."
      Honma S., Kawamoto T., Takagi Y., Fujimoto K., Sato F., Noshiro M., Kato Y., Honma K.I.
      Nature 419:841-844(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ARNTL.
    3. Cited for: FUNCTION.
    4. "Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation. Interaction with BMAL1."
      Sato F., Kawamoto T., Fujimoto K., Noshiro M., Honda K.K., Honma S., Honma K., Kato Y.
      Eur. J. Biochem. 271:4409-4419(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: HETERODIMERIZATION WITH BHLHE40.
    5. Cited for: FUNCTION.
    6. "The basic helix-loop-helix proteins differentiated embryo chondrocyte (DEC) 1 and DEC2 function as corepressors of retinoid X receptors."
      Cho Y., Noshiro M., Choi M., Morita K., Kawamoto T., Fujimoto K., Kato Y., Makishima M.
      Mol. Pharmacol. 76:1360-1369(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    7. "The transcriptional repressor DEC2 regulates sleep length in mammals."
      He Y., Jones C.R., Fujiki N., Xu Y., Guo B., Holder J.L. Jr., Rossner M.J., Nishino S., Fu Y.H.
      Science 325:866-870(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: TRANSGENIC MICE.
    8. Cited for: FUNCTION, INTERACTION WITH CIART.

    Entry informationi

    Entry nameiBHE41_MOUSE
    AccessioniPrimary (citable) accession number: Q99PV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Activity profiles and sleep recordings of transgenic mice carrying the mutation Arg-325 show increased vigilance time and less sleep time than control mice in a zeitgeber time- and sleep deprivation-dependent manner.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3