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Q99PV5

- BHE41_MOUSE

UniProt

Q99PV5 - BHE41_MOUSE

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Protein
Class E basic helix-loop-helix protein 41
Gene
Bhlhe41, Bhlhb3, Dec2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.5 Publications

GO - Molecular functioni

  1. E-box binding Source: BHF-UCL
  2. MRF binding Source: BHF-UCL
  3. RNA polymerase II activating transcription factor binding Source: BHF-UCL
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: BHF-UCL
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
  6. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  7. RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Source: BHF-UCL
  8. bHLH transcription factor binding Source: BHF-UCL
  9. histone deacetylase binding Source: BHF-UCL
  10. protein binding Source: UniProtKB
  11. protein heterodimerization activity Source: BHF-UCL
  12. protein homodimerization activity Source: BHF-UCL
  13. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: BHF-UCL
  14. transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. circadian regulation of gene expression Source: BHF-UCL
  2. circadian rhythm Source: UniProtKB
  3. negative regulation of myotube differentiation Source: BHF-UCL
  4. negative regulation of transcription by competitive promoter binding Source: BHF-UCL
  5. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  6. negative regulation of transcription, DNA-templated Source: UniProtKB
  7. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
REACT_198620. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Class E basic helix-loop-helix protein 41
Short name:
bHLHe41
Alternative name(s):
Class B basic helix-loop-helix protein 3
Short name:
bHLHb3
Differentially expressed in chondrocytes protein 2
Short name:
mDEC2
Gene namesi
Name:Bhlhe41
Synonyms:Bhlhb3, Dec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1930704. Bhlhe41.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
  2. transcriptional repressor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Class E basic helix-loop-helix protein 41
PRO_0000127148Add
BLAST

Proteomic databases

PRIDEiQ99PV5.

PTM databases

PhosphoSiteiQ99PV5.

Expressioni

Tissue specificityi

Expressed in skeletal muscle, brain and lung.

Inductioni

Expressed in a circdadian manner in the liver with a peak at ZT10.1 Publication

Gene expression databases

ArrayExpressiQ99PV5.
BgeeiQ99PV5.
GenevestigatoriQ99PV5.

Interactioni

Subunit structurei

Homodimer. Interacts with CIART. Interacts with ARNTL/BMAL1 and RXRA.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CebpbP280335EBI-6143801,EBI-1029979

Protein-protein interaction databases

BioGridi219755. 3 interactions.
DIPiDIP-57696N.
IntActiQ99PV5. 21 interactions.
MINTiMINT-7012655.
STRINGi10090.ENSMUSP00000032386.

Structurei

3D structure databases

ProteinModelPortaliQ99PV5.
SMRiQ99PV5. Positions 36-100.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 9956bHLH
Add
BLAST
Domaini131 – 16636Orange
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi321 – 37353Ala/Gly-rich
Add
BLAST

Sequence similaritiesi

Contains 1 Orange domain.

Phylogenomic databases

eggNOGiNOG235394.
GeneTreeiENSGT00510000046898.
HOGENOMiHOG000234381.
InParanoidiQ99PV5.
KOiK03730.
OMAiAYKLPHR.
OrthoDBiEOG75XGKZ.
PhylomeDBiQ99PV5.
TreeFamiTF330859.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange.
IPR018352. Orange_subgr.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99PV5-1 [UniParc]FASTAAdd to Basket

« Hide

MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR    50
LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT 100
ALTEQQHQKI IALQNGERSL KSPVQADLDA FHSGFQTCAK EVLQYLARFE 150
SWTPREPRCA QLVSHLHAVA TQLLTPQVPS GRGSGRAPCS AGAAAASGPE 200
RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK QEPPGDSSPA 250
PKRPKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS 300
AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS 350
VLSPPPEKAG ATAGAPFLAH EVAPPGPLRP QHAHSRTHLP RAVNPESSQE 400
DATQPAKDAP 410
Length:410
Mass (Da):43,946
Last modified:June 1, 2001 - v1
Checksum:i40A87281B08E233D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB044090 mRNA. Translation: BAB21503.1.
CCDSiCCDS20697.1.
PIRiJC7584.
RefSeqiNP_001258697.1. NM_001271768.1.
NP_077789.1. NM_024469.2.
UniGeneiMm.154529.

Genome annotation databases

EnsembliENSMUST00000032386; ENSMUSP00000032386; ENSMUSG00000030256.
GeneIDi79362.
KEGGimmu:79362.
UCSCiuc009ert.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB044090 mRNA. Translation: BAB21503.1 .
CCDSi CCDS20697.1.
PIRi JC7584.
RefSeqi NP_001258697.1. NM_001271768.1.
NP_077789.1. NM_024469.2.
UniGenei Mm.154529.

3D structure databases

ProteinModelPortali Q99PV5.
SMRi Q99PV5. Positions 36-100.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 219755. 3 interactions.
DIPi DIP-57696N.
IntActi Q99PV5. 21 interactions.
MINTi MINT-7012655.
STRINGi 10090.ENSMUSP00000032386.

PTM databases

PhosphoSitei Q99PV5.

Proteomic databases

PRIDEi Q99PV5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032386 ; ENSMUSP00000032386 ; ENSMUSG00000030256 .
GeneIDi 79362.
KEGGi mmu:79362.
UCSCi uc009ert.1. mouse.

Organism-specific databases

CTDi 79365.
MGIi MGI:1930704. Bhlhe41.

Phylogenomic databases

eggNOGi NOG235394.
GeneTreei ENSGT00510000046898.
HOGENOMi HOG000234381.
InParanoidi Q99PV5.
KOi K03730.
OMAi AYKLPHR.
OrthoDBi EOG75XGKZ.
PhylomeDBi Q99PV5.
TreeFami TF330859.

Enzyme and pathway databases

Reactomei REACT_115781. Bmal1:Clock,Npas2 activates circadian gene expression.
REACT_198620. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

Miscellaneous databases

ChiTaRSi BHLHE41. mouse.
NextBioi 349911.
PROi Q99PV5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99PV5.
Bgeei Q99PV5.
Genevestigatori Q99PV5.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR003650. Orange.
IPR018352. Orange_subgr.
[Graphical view ]
Pfami PF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of DEC2, a new member of basic helix-loop-helix proteins."
    Fujimoto K., Shen M., Noshiro M., Matsubara K., Shingu S., Honda K., Yoshida E., Suardita K., Matsuda Y., Kato Y.
    Biochem. Biophys. Res. Commun. 280:164-171(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Dec1 and Dec2 are regulators of the mammalian molecular clock."
    Honma S., Kawamoto T., Takagi Y., Fujimoto K., Sato F., Noshiro M., Kato Y., Honma K.I.
    Nature 419:841-844(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARNTL.
  3. Cited for: FUNCTION.
  4. "Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation. Interaction with BMAL1."
    Sato F., Kawamoto T., Fujimoto K., Noshiro M., Honda K.K., Honma S., Honma K., Kato Y.
    Eur. J. Biochem. 271:4409-4419(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: HETERODIMERIZATION WITH BHLHE40.
  5. Cited for: FUNCTION.
  6. "The basic helix-loop-helix proteins differentiated embryo chondrocyte (DEC) 1 and DEC2 function as corepressors of retinoid X receptors."
    Cho Y., Noshiro M., Choi M., Morita K., Kawamoto T., Fujimoto K., Kato Y., Makishima M.
    Mol. Pharmacol. 76:1360-1369(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  7. "The transcriptional repressor DEC2 regulates sleep length in mammals."
    He Y., Jones C.R., Fujiki N., Xu Y., Guo B., Holder J.L. Jr., Rossner M.J., Nishino S., Fu Y.H.
    Science 325:866-870(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSGENIC MICE.
  8. Cited for: FUNCTION, INTERACTION WITH CIART.

Entry informationi

Entry nameiBHE41_MOUSE
AccessioniPrimary (citable) accession number: Q99PV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Activity profiles and sleep recordings of transgenic mice carrying the mutation Arg-325 show increased vigilance time and less sleep time than control mice in a zeitgeber time- and sleep deprivation-dependent manner.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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