Q99PV3 (MKLN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Muskelin

Gene names

Name:	Mkln1
Synonyms:	Msk

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1 By similarity.
Subunit structure	Interacts with RANBP9. Part of a complex consisting of RANBP9, MKLN1 and GID8 By similarity. Interacts with the C-terminal tail of the prostaglandin EP3 receptor alpha isoform. Ref.1
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 CTLH domain. Contains 6 Kelch repeats. Contains 1 LisH domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Kelch repeat Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Muskelin		PRO_0000119140
Regions
	Domain	172 – 204	33	LisH
	Domain	206 – 258	53	CTLH
	Repeat	284 – 330	47	Kelch 1
	Repeat	339 – 391	53	Kelch 2
	Repeat	400 – 458	59	Kelch 3
	Repeat	469 – 515	47	Kelch 4
	Repeat	526 – 578	53	Kelch 5
	Repeat	597 – 651	55	Kelch 6

Sequences

	Sequence		Length	Mass (Da)
	Q99PV3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 79BD0BBF74419E76	FASTA	735	84,834
	10 20 30 40 50 60 MAAGGAVAAA PECRLLPYAL HKWSSFSSTY LPENILVDKP NDQSSRWSSE SNYPPQYLIL 70 80 90 100 110 120 KLERPAIVQN ITFGKYEKTH VCNLKKFKVF GGMNEENMTE LLSSGLKNDY NKETFTLKHK 130 140 150 160 170 180 IDEQMFPCRF IKIVPLLSWG PSFNFSIWYV ELSGIDDPDI VQPCLNWYSK YREQEAIRLC 190 200 210 220 230 240 LKHFRQHNYT EAFESLQKKT KIALEHPMLT DMHDKLVLKG DFDACEELIE KAVNDGLFNQ 250 260 270 280 290 300 YISQQEYKPR WSQIIPKSTK GDGEDNRPGM RGGHQMVIDV QTETVYLFGG WDGTQDLADF 310 320 330 340 350 360 WAYSVKENQW TCISRDTEKE NGPSARSCHK MCIDIQRRQI YTLGRYLDSS VRNSKSLKSD 370 380 390 400 410 420 FYRYDIDTNT WMLLSEDTAA DGGPKLVFDH QMCMDSEKHM IYTFGGRILT CNGSVDDSRA 430 440 450 460 470 480 SEPQFSGLFA FNCQCQTWKL LREDSCNAGP EDIQSRIGHC MLFHSKNRCL YVFGGQRSKT 490 500 510 520 530 540 YLNDFFSYDV DSDHVDIISD GTKKDSGMVP MTGFTQRATI DPELNEIHVL SGLSKDKEKR 550 560 570 580 590 600 EENVRNSFWI YDIVRNSWSC VYKNDQAAKE NLSKSLQEEE PCPRFAHQLV YDELHKVHYL 610 620 630 640 650 660 FGGNPGKSCS PKMRLDDFWS LKLCRPSKDY LLRHCKYLIR KHRFEEKAQM DPLSALKYLQ 670 680 690 700 710 720 NDLYITVDHS DPEETKEFQL LASALFKSGS DFTALGFSDV DHTYAQRTQL FDTLVNFFPD 730 SMTPPKGNLV DLITL

References

[1]	"Receptor isoform-specific interaction of prostaglandin EP3 receptor with muskelin." Hasegawa H., Katoh H., Fujita H., Mori K., Negishi M. Biochem. Biophys. Res. Commun. 276:350-354(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH EP3-ALPHA.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046442 mRNA. Translation: BAB21439.1.
IPI	IPI00201434.
RefSeq	NP_112649.1. NM_031359.1.
UniGene	Rn.6383.
3D structure databases
ProteinModelPortal	Q99PV3.
ModBase	Search...
Proteomic databases
PaxDb	Q99PV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016633; ENSRNOP00000016633; ENSRNOG00000011524.
GeneID	83536.
KEGG	rno:83536.
UCSC	RGD:620076. rat.
Organism-specific databases
CTD	4289.
RGD	620076. Mkln1.
Phylogenomic databases
eggNOG	NOG290166.
GeneTree	ENSGT00390000001702.
HOGENOM	HOG000047912.
HOVERGEN	HBG052481.
InParanoid	Q99PV3.
OrthoDB	EOG4NP72V.
Gene expression databases
Genevestigator	Q99PV3.
GermOnline	ENSRNOG00000011524. Rattus norvegicus.
Family and domain databases
Gene3D	2.120.10.80. 1 hit. 2.60.120.260. 1 hit.
InterPro	IPR006595. CTLH_C. IPR008979. Galactose-bd-like. IPR015915. Kelch-typ_b-propeller. IPR006594. LisH_dimerisation. IPR010565. Muskelin_N. [Graphical view]
Pfam	PF06588. Muskelin_N. 1 hit. [Graphical view]
SMART	SM00668. CTLH. 1 hit. SM00667. LisH. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit.
PROSITE	PS50897. CTLH. 1 hit. PS50896. LISH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	616045.

Entry information

Entry name

MKLN1_RAT

Accession

Primary (citable) accession number: Q99PV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program