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Protein

Muskelin

Gene

Mkln1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1.By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Muskelin
Gene namesi
Name:Mkln1
Synonyms:Msk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi620076. Mkln1.

Subcellular locationi

Cytoplasm By similarity. Cell projectionruffle By similarity. Cytoplasmcell cortex By similarity
Note: Colocalizes with actin fibers in the cell cortex.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 735734MuskelinPRO_0000119140Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ99PV3.
PRIDEiQ99PV3.

Expressioni

Gene expression databases

GenevestigatoriQ99PV3.

Interactioni

Subunit structurei

Interacts with RANBP9. Part of a complex consisting of RANBP9, MKLN1 and GID8 (By similarity). Interacts with the C-terminal tail of the prostaglandin EP3 receptor alpha isoform.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016633.

Structurei

3D structure databases

ProteinModelPortaliQ99PV3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 20433LisHPROSITE-ProRule annotationAdd
BLAST
Domaini206 – 25853CTLHPROSITE-ProRule annotationAdd
BLAST
Repeati284 – 33047Kelch 1Add
BLAST
Repeati339 – 39153Kelch 2Add
BLAST
Repeati400 – 45859Kelch 3Add
BLAST
Repeati469 – 51547Kelch 4Add
BLAST
Repeati526 – 57853Kelch 5Add
BLAST
Repeati597 – 65155Kelch 6Add
BLAST

Sequence similaritiesi

Contains 1 CTLH domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated
Contains 1 LisH domain.PROSITE-ProRule annotation

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiNOG290166.
GeneTreeiENSGT00390000001702.
HOGENOMiHOG000047912.
HOVERGENiHBG052481.
InParanoidiQ99PV3.
OMAiWIYDIAR.
OrthoDBiEOG7CNZFQ.
PhylomeDBiQ99PV3.
TreeFamiTF323659.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR006595. CTLH_C.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR008979. Galactose-bd-like.
IPR015915. Kelch-typ_b-propeller.
IPR006594. LisH_dimerisation.
IPR010565. Muskelin_N.
[Graphical view]
PfamiPF06588. Muskelin_N. 1 hit.
[Graphical view]
SMARTiSM00668. CTLH. 1 hit.
SM00667. LisH. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50965. SSF50965. 1 hit.
PROSITEiPS50897. CTLH. 1 hit.
PS50896. LISH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PV3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAGGAVAAA PECRLLPYAL HKWSSFSSTY LPENILVDKP NDQSSRWSSE
60 70 80 90 100
SNYPPQYLIL KLERPAIVQN ITFGKYEKTH VCNLKKFKVF GGMNEENMTE
110 120 130 140 150
LLSSGLKNDY NKETFTLKHK IDEQMFPCRF IKIVPLLSWG PSFNFSIWYV
160 170 180 190 200
ELSGIDDPDI VQPCLNWYSK YREQEAIRLC LKHFRQHNYT EAFESLQKKT
210 220 230 240 250
KIALEHPMLT DMHDKLVLKG DFDACEELIE KAVNDGLFNQ YISQQEYKPR
260 270 280 290 300
WSQIIPKSTK GDGEDNRPGM RGGHQMVIDV QTETVYLFGG WDGTQDLADF
310 320 330 340 350
WAYSVKENQW TCISRDTEKE NGPSARSCHK MCIDIQRRQI YTLGRYLDSS
360 370 380 390 400
VRNSKSLKSD FYRYDIDTNT WMLLSEDTAA DGGPKLVFDH QMCMDSEKHM
410 420 430 440 450
IYTFGGRILT CNGSVDDSRA SEPQFSGLFA FNCQCQTWKL LREDSCNAGP
460 470 480 490 500
EDIQSRIGHC MLFHSKNRCL YVFGGQRSKT YLNDFFSYDV DSDHVDIISD
510 520 530 540 550
GTKKDSGMVP MTGFTQRATI DPELNEIHVL SGLSKDKEKR EENVRNSFWI
560 570 580 590 600
YDIVRNSWSC VYKNDQAAKE NLSKSLQEEE PCPRFAHQLV YDELHKVHYL
610 620 630 640 650
FGGNPGKSCS PKMRLDDFWS LKLCRPSKDY LLRHCKYLIR KHRFEEKAQM
660 670 680 690 700
DPLSALKYLQ NDLYITVDHS DPEETKEFQL LASALFKSGS DFTALGFSDV
710 720 730
DHTYAQRTQL FDTLVNFFPD SMTPPKGNLV DLITL
Length:735
Mass (Da):84,834
Last modified:June 1, 2001 - v1
Checksum:i79BD0BBF74419E76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046442 mRNA. Translation: BAB21439.1.
RefSeqiNP_112649.1. NM_031359.1.
UniGeneiRn.6383.

Genome annotation databases

EnsembliENSRNOT00000016633; ENSRNOP00000016633; ENSRNOG00000011524.
GeneIDi83536.
KEGGirno:83536.
UCSCiRGD:620076. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046442 mRNA. Translation: BAB21439.1.
RefSeqiNP_112649.1. NM_031359.1.
UniGeneiRn.6383.

3D structure databases

ProteinModelPortaliQ99PV3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016633.

Proteomic databases

PaxDbiQ99PV3.
PRIDEiQ99PV3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016633; ENSRNOP00000016633; ENSRNOG00000011524.
GeneIDi83536.
KEGGirno:83536.
UCSCiRGD:620076. rat.

Organism-specific databases

CTDi4289.
RGDi620076. Mkln1.

Phylogenomic databases

eggNOGiNOG290166.
GeneTreeiENSGT00390000001702.
HOGENOMiHOG000047912.
HOVERGENiHBG052481.
InParanoidiQ99PV3.
OMAiWIYDIAR.
OrthoDBiEOG7CNZFQ.
PhylomeDBiQ99PV3.
TreeFamiTF323659.

Miscellaneous databases

NextBioi616045.
PROiQ99PV3.

Gene expression databases

GenevestigatoriQ99PV3.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR006595. CTLH_C.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR008979. Galactose-bd-like.
IPR015915. Kelch-typ_b-propeller.
IPR006594. LisH_dimerisation.
IPR010565. Muskelin_N.
[Graphical view]
PfamiPF06588. Muskelin_N. 1 hit.
[Graphical view]
SMARTiSM00668. CTLH. 1 hit.
SM00667. LisH. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF50965. SSF50965. 1 hit.
PROSITEiPS50897. CTLH. 1 hit.
PS50896. LISH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Receptor isoform-specific interaction of prostaglandin EP3 receptor with muskelin."
    Hasegawa H., Katoh H., Fujita H., Mori K., Negishi M.
    Biochem. Biophys. Res. Commun. 276:350-354(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH EP3-ALPHA.

Entry informationi

Entry nameiMKLN1_RAT
AccessioniPrimary (citable) accession number: Q99PV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.