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Protein

Pre-mRNA-processing-splicing factor 8

Gene

Prpf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing-splicing factor 8
Alternative name(s):
Splicing factor Prp8
Gene namesi
Name:Prpf8
Synonyms:Prp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2179381 Prpf8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000970412 – 2335Pre-mRNA-processing-splicing factor 8Add BLAST2334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei859PhosphoserineBy similarity1
Modified residuei1358PhosphoserineBy similarity1
Modified residuei1425N6,N6-dimethyllysineBy similarity1
Modified residuei1463N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ99PV0
MaxQBiQ99PV0
PaxDbiQ99PV0
PeptideAtlasiQ99PV0
PRIDEiQ99PV0

PTM databases

iPTMnetiQ99PV0
PhosphoSitePlusiQ99PV0
SwissPalmiQ99PV0

Expressioni

Tissue specificityi

Strongly expressed in testis (preferentially in the outer cell layer), and moderately in ovary (preferentially in granulosa cells).1 Publication

Developmental stagei

During embryogenesis, is highly expressed at day 9.5 of gestation, and its expression decreases progressively during embryogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000020850
CleanExiMM_PRPF8
ExpressionAtlasiQ99PV0 baseline and differential
GenevisibleiQ99PV0 MM

Interactioni

Subunit structurei

Part of the U5 snRNP complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of the U5.U4atac/U6atac snRNP complexes in U12-dependent spliceosomes. Found in a mRNA splicing-dependent exon junction complex (EJC) with SRRM1. Interacts with U5 snRNP proteins SNRP116 and SNRNP40. Interacts with EFTUD2 and SNRNP200. Interacts (via the MPN (JAB/Mov34) domain) with PRPF3 ('Lys-63'-linked polyubiquitinated); may stabilize the U4/U6-U5 tri-snRNP complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228650, 56 interactors
IntActiQ99PV0, 59 interactors
MINTiQ99PV0
STRINGi10090.ENSMUSP00000018449

Structurei

3D structure databases

ProteinModelPortaliQ99PV0
SMRiQ99PV0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2103 – 2234MPNPROSITE-ProRule annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni812 – 1303Reverse transcriptase homology domainAdd BLAST492
Regioni1304 – 1577LinkerAdd BLAST274
Regioni1513 – 1526Important for branch point selectionBy similarityAdd BLAST14
Regioni1581 – 1752Restriction endonuclease homology domainAdd BLAST172
Regioni1669 – 2034Involved in interaction with pre-mRNA 5' splice siteBy similarityAdd BLAST366
Regioni1767 – 2020RNase H homology domainAdd BLAST254
Regioni2301 – 2335Required for interaction with EFTUD2 and SNRNP200By similarityAdd BLAST35

Domaini

The MPN (JAB/Mov34) domain has structural similarity with deubiquitinating enzymes, but lacks the residues that would bind the catalytic metal ion.By similarity
Contains a region with structural similarity to reverse transcripase, presenting the classical thumb, fingers and palm architecture, but lacks enzyme activity, since the essential metal-binding residues are not conserved.By similarity
Contains a region with structural similarity to type-2 restriction endonucleases, but the residues that would bind catalytic metal ions in endonucleases are instead involved in hydrogen bonds that stabilize the protein structure.By similarity
Contains a region with structural similarity to RNase H, but lacks RNase H activity.By similarity

Phylogenomic databases

eggNOGiKOG1795 Eukaryota
COG5178 LUCA
GeneTreeiENSGT00390000015210
HOVERGENiHBG052796
InParanoidiQ99PV0
KOiK12856
OMAiQNKVNTV
OrthoDBiEOG091G003B
PhylomeDBiQ99PV0
TreeFamiTF105613

Family and domain databases

CDDicd13838 RNase_H_like_Prp8_IV, 1 hit
InterProiView protein in InterPro
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
IPR012591 PRO8NT
IPR012592 PROCN
IPR012984 PROCT
IPR027652 PRP8
IPR021983 PRP8_domainIV
IPR019581 Prp8_U5-snRNA-bd
IPR019580 Prp8_U6-snRNA-bd
IPR012337 RNaseH-like_sf
IPR019582 RRM_spliceosomal_PrP8
PANTHERiPTHR11140 PTHR11140, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
PF08082 PRO8NT, 1 hit
PF08083 PROCN, 1 hit
PF08084 PROCT, 1 hit
PF12134 PRP8_domainIV, 1 hit
PF10598 RRM_4, 1 hit
PF10597 U5_2-snRNA_bdg, 1 hit
PF10596 U6-snRNA_bdg, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD149576 Pre-mRNA-splicing_factor-8, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
SUPFAMiSSF53098 SSF53098, 2 hits
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK
60 70 80 90 100
FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYLG ALKYMPHAVL
110 120 130 140 150
KLLENMPMPW EQIRDVPVLY HITGAISFVN EIPWVIEPVY ISQWGSMWIM
160 170 180 190 200
MRREKRDRRH FKRMRFPPFD DEEPPLDYAD NILDVEPLEA IQLELDPEED
210 220 230 240 250
APVLDWFYDH QPLRDSRKYV NGSTYQRWQF TLPMMSTLYR LANQLLTDLV
260 270 280 290 300
DDNYFYLFDL KAFFTSKALN MAIPGGPKFE PLVRDINLQD EDWNEFNDIN
310 320 330 340 350
KIIIRQPIRT EYKIAFPYLY NNLPHHVHLT WYHTPNVVFI KTEDPDLPAF
360 370 380 390 400
YFDPLINPIS HRHSVKSQEP LPDDDEEFEL PEFVEPFLKD TPLYTDNTAN
410 420 430 440 450
GIALLWAPRP FNLRSGRTRR ALDIPLVKNW YREHCPAGQP VKVRVSYQKL
460 470 480 490 500
LKYYVLNALK HRPPKAQKKR YLFRSFKATK FFQSTKLDWV EVGLQVCRQG
510 520 530 540 550
YNMLNLLIHR KNLNYLHLDY NFNLKPVKTL TTKERKKSRF GNAFHLCREV
560 570 580 590 600
LRLTKLVVDS HVQYRLGNVD AFQLADGLQY IFAHVGQLTG MYRYKYKLMR
610 620 630 640 650
QIRMCKDLKH LIYYRFNTGP VGKGPGCGFW AAGWRVWLFF MRGITPLLER
660 670 680 690 700
WLGNLLARQF EGRHSKGVAK TVTKQRVESH FDLELRAAVM HDILDMMPEG
710 720 730 740 750
IKQNKARTIL QHLSEAWRCW KANIPWKVPG LPTPIENMIL RYVKAKADWW
760 770 780 790 800
TNTAHYNRER IRRGATVDKT VCKKNLGRLT RLYLKAEQER QHNYLKDGPY
810 820 830 840 850
ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY
860 870 880 890 900
SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQRA FKEVGIEFMD
910 920 930 940 950
LYSHLVPVYD VEPLEKITDA YLDQYLWYEA DKRRLFPPWI KPADTEPPPL
960 970 980 990 1000
LVYKWCQGIN NLQDVWETSE GECNVMLESR FEKMYEKIDL TLLNRLLRLI
1010 1020 1030 1040 1050
VDHNIADYMT AKNNVVINYK DMNHTNSYGI IRGLQFASFI VQYYGLVMDL
1060 1070 1080 1090 1100
LVLGLHRASE MAGPPQMPND FLSFQDIATE AAHPIRLFCR YIDRIHIFFR
1110 1120 1130 1140 1150
FTADEARDLI QRYLTEHPDP NNENIVGYNN KKCWPRDARM RLMKHDVNLG
1160 1170 1180 1190 1200
RAVFWDIKNR LPRSVTTVQW ENSFVSVYSK DNPNLLFNMC GFECRILPKC
1210 1220 1230 1240 1250
RTSYEEFTHK DGVWNLQNEV TKERTAQCFL RVDDESMQRF HNRVRQILMA
1260 1270 1280 1290 1300
SGSTTFTKIV NKWNTALIGL MTYFREAVVN TQELLDLLVK CENKIQTRIK
1310 1320 1330 1340 1350
IGLNSKMPSR FPPVVFYTPK ELGGLGMLSM GHVLIPQSDL RWSKQTDVGI
1360 1370 1380 1390 1400
THFRSGMSHE EDQLIPNLYR YIQPWESEFI DSQRVWAEYA LKRQEAIAQN
1410 1420 1430 1440 1450
RRLTLEDLED SWDRGIPRIN TLFQKDRHTL AYDKGWRVRT DFKQYQVLKQ
1460 1470 1480 1490 1500
NPFWWTHQRH DGKLWNLNNY RTDMIQALGG VEGILEHTLF KGTYFPTWEG
1510 1520 1530 1540 1550
LFWEKASGFE ESMKWKKLTN AQRSGLNQIP NRRFTLWWSP TINRANVYVG
1560 1570 1580 1590 1600
FQVQLDLTGI FMHGKIPTLK ISLIQIFRAH LWQKIHESIV MDLCQVFDQE
1610 1620 1630 1640 1650
LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD
1660 1670 1680 1690 1700
VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTTD NMSIYPSPTG
1710 1720 1730 1740 1750
VLIAIDLAYN LHSAYGNWFP GSKPLIQQAM AKIMKANPAL YVLRERIRKG
1760 1770 1780 1790 1800
LQLYSSEPTE PYLSSQNYGE LFSNQIIWFV DDTNVYRVTI HKTFEGNLTT
1810 1820 1830 1840 1850
KPINGAIFIF NPRTGQLFLK IIHTSVWAGQ KRLGQLAKWK TAEEVAALIR
1860 1870 1880 1890 1900
SLPVEEQPKQ IIVTRKGMLD PLEVHLLDFP NIVIKGSELQ LPFQACLKVE
1910 1920 1930 1940 1950
KFGDLILKAT EPQMVLFNLY DDWLKTISSY TAFSRLILIL RALHVNNDRA
1960 1970 1980 1990 2000
KVILKPDKTT VTEPHHIWPT LTDEEWIKVE VQLKDLILAD YGKKNNVNVA
2010 2020 2030 2040 2050
SLTQSEIRDI ILGMEISAPS QQRQQIAEIE KQTKEQSQLT ATQTRTVNKH
2060 2070 2080 2090 2100
GDEIITSTTS NYETQTFSSK TEWRVRAISA ANLHLRTNHI YVSSDDIKET
2110 2120 2130 2140 2150
GYTYILPKNV LKKFICISDL RAQIAGYLYG VSPPDNPQVK EIRCIVMVPQ
2160 2170 2180 2190 2200
WGTHQTVHLP SQLPQHEYLK EMEPLGWIHT QPNESPQLSP QDVTTHAKIM
2210 2220 2230 2240 2250
ADNPSWDGEK TIIITCSFTP GSCTLTAYKL TPSGYEWGRQ NTDKGNNPKG
2260 2270 2280 2290 2300
YLPSHYERVQ MLLSDRFLGF FMVPAQSSWN YNFMGVRHDP NMKYELQLAN
2310 2320 2330
PKEFYHEVHR PSHFLNFALL QEGEVYSADR EDLYA
Length:2,335
Mass (Da):273,616
Last modified:November 24, 2009 - v2
Checksum:iC1A7A989B1AFB3B7
GO

Sequence cautioni

The sequence AAH54103 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti797D → V in BAB32671 (PubMed:11275560).Curated1
Sequence conflicti2191 – 2195QDVTT → THASA in AAH54103 (PubMed:15489334).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047391 mRNA Translation: BAB32671.1
AL591496 Genomic DNA No translation available.
BC034648 mRNA Translation: AAH34648.1
BC054103 mRNA Translation: AAH54103.1 Different initiation.
BC093481 mRNA Translation: AAH93481.1
AK041017 mRNA Translation: BAC30783.1
CCDSiCCDS25048.1
RefSeqiNP_619600.2, NM_138659.2
UniGeneiMm.3757

Genome annotation databases

EnsembliENSMUST00000018449; ENSMUSP00000018449; ENSMUSG00000020850
ENSMUST00000102510; ENSMUSP00000099568; ENSMUSG00000020850
GeneIDi192159
KEGGimmu:192159
UCSCiuc007kdx.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPRP8_MOUSE
AccessioniPrimary (citable) accession number: Q99PV0
Secondary accession number(s): A5D6Q4
, Q5ND30, Q5ND31, Q7TQK2, Q8BRZ5, Q8K001
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: November 24, 2009
Last modified: June 20, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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