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Protein

Putative ATP-dependent RNA helicase DHX30

Gene

Dhx30

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the assembly of the mitochondrial large ribosomal subunit. Associates with mitochondrial DNA. Required for optimal function of the zinc-finger antiviral protein ZC3HAV1.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi480 – 4878ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase DHX30 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 30
Gene namesi
Name:Dhx30
Synonyms:Helg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1920081. Dhx30.

Subcellular locationi

Isoform 2 :
Isoform 1 :
  • Mitochondrion matrixmitochondrion nucleoid By similarity
  • Cytoplasm Curated

  • Note: Localizes to mitochondrial RNA granules found in close proximity to the mitochondrial nucleoids.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12171217Putative ATP-dependent RNA helicase DHX30PRO_0000245539Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291PhosphoserineBy similarity
Modified residuei249 – 2491PhosphoserineCombined sources
Modified residuei403 – 4031PhosphoserineBy similarity
Isoform 3 (identifier: Q99PU8-3)
Modified residuei15 – 151PhosphoserineBy similarity

Post-translational modificationi

Isoform 3 is phosphorylated on Ser-15.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99PU8.
PaxDbiQ99PU8.
PeptideAtlasiQ99PU8.
PRIDEiQ99PU8.

PTM databases

iPTMnetiQ99PU8.
PhosphoSiteiQ99PU8.
SwissPalmiQ99PU8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032480.
CleanExiMM_DHX30.
ExpressionAtlasiQ99PU8. baseline and differential.
GenevisibleiQ99PU8. MM.

Interactioni

Subunit structurei

Identified in a complex with TFAM and SSBP1. Interacts (via N-terminus) with ZC3HAV1 (via N-terminal domain) in an RNA-independent manner. Found in a complex with GRSF1, DDX28, FASTKD2 and FASTKD5.By similarity

Protein-protein interaction databases

BioGridi215593. 1 interaction.
IntActiQ99PU8. 2 interactions.
MINTiMINT-1861298.
STRINGi10090.ENSMUSP00000062622.

Structurei

3D structure databases

ProteinModelPortaliQ99PU8.
SMRiQ99PU8. Positions 67-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 14469DRBMAdd
BLAST
Domaini467 – 635169Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini677 – 850174Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi582 – 5854DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 22212Poly-GluAdd
BLAST
Compositional biasi1033 – 10364Poly-Glu

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000112212.
HOVERGENiHBG081437.
InParanoidiQ99PU8.
KOiK13185.
OMAiPLTHAMY.
OrthoDBiEOG091G01RZ.
PhylomeDBiQ99PU8.
TreeFamiTF352030.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99PU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVTPVCNSST WQPKDSSFLS WPEMFSLDSF RKDRTQHRQR QCKLPPPRLP
60 70 80 90 100
PMCVNPAPGG TITRASRDLL KEFPQPKNLL NSVIGRALGI SHAKDKLVYV
110 120 130 140 150
HTNGPKKKKV TLHIKWPKSV EVEGYGSKKI DAERQAAAAA CQLFKGWGLL
160 170 180 190 200
GPRNELFDAA KYRVLADRFG SPADSWWRPE PTMPPTSWRQ LNPENIRPGG
210 220 230 240 250
PAGLSRSLGR EEEEDEEEEL EEGTIDVTEF LSMTQQDSHN PLRDSRGGSF
260 270 280 290 300
EMTDDDSAIR ALTQFPLPKN LLAKVIQIAT SSSTAKNLMQ FHTVGTKTKL
310 320 330 340 350
ATLTLLWPCP MTFVAKGRRK AEAENKAAAL ACKKLKSLGL VDRNNEPLTH
360 370 380 390 400
AMYNLASLRE LGETQRRPCT IQVPEPILRK IEAFLSHYPV DSSWISPELR
410 420 430 440 450
LQSDDILPLG KDSGPLSDPI TGKPYMPLSE AEEVRLSQSL LELWRRRGPI
460 470 480 490 500
WQEAPQLPVD PHRDTILSAI EQHPVVVISG DTGCGKTTRI PQLLLERYVT
510 520 530 540 550
EGRGARCNVI ITQPRRISAV SVAQRVSHEL GPSLRRNVGF QVRLESKPPA
560 570 580 590 600
RGGALLFCTV GILLRKLQSN PSLEGVSHVI VDEVHERDVN TDFLLILLKG
610 620 630 640 650
LQRLNPALRL VLMSATGDNE RFSRYFGGCP VIKVPGFMYP VKEHYLEDIL
660 670 680 690 700
AKLGKHQYPH RHRHHESEDE CALDLDLVTD LVLHIDARGE PGGILCFLPG
710 720 730 740 750
WQEIKGVQQR LQEALGMHES KYLILPVHSN IPMMDQKAIF QQPPLGVRKI
760 770 780 790 800
VLATNIAETS ITVNDIVHVV DSGLHKEERY DLKTKVSCLE TVWVSRANVI
810 820 830 840 850
QRRGRAGRCQ SGFAYHLFPR SRLEKMVPFQ VPEILRTPLE NLVLQAKIHM
860 870 880 890 900
PEKTAVEFLS KAVDSPNIKA VDEAVILLQE IGVLDQREYL TTLGQRLAHI
910 920 930 940 950
STDPRLAKAI VLAAIFRCLH PLLVVVSCLT RDPFSSSLQN RAEVDKVKAL
960 970 980 990 1000
LSHDSGSDHL AFVRAVAGWE EVLRWQDRTS RENYLEENLL YAPSLRFIHG
1010 1020 1030 1040 1050
LIKQFSENIY EAFLVGKPSD CTLPSAQCNE YSEEEELVKG VLMAGLYPNL
1060 1070 1080 1090 1100
IQVRQGKVTR QGKFKPNSVT YRTKSGNILL HKSTINREAT RLRSRWLTYF
1110 1120 1130 1140 1150
MAVKSNGSVF VRDSSQVHPL AVLLLTDGDV HIRDDGRRAT ISLSDSDLLR
1160 1170 1180 1190 1200
LEGDSRTVRL LREFRRALGR MVERSLRSEL AALPLSVQQE HGQLLALLAE
1210
LLRGPCGSFD MRKTADD
Length:1,217
Mass (Da):136,668
Last modified:June 1, 2001 - v1
Checksum:iC6157413C98A1200
GO
Isoform 2 (identifier: Q99PU8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Note: No experimental confirmation available.
Show »
Length:1,194
Mass (Da):134,059
Checksum:iCC8CAA05C68C1CF8
GO
Isoform 3 (identifier: Q99PU8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: MVTPVCNSST...NPAPGGTITR → MAAARRLMAL...PGEGDGSMVN

Show »
Length:1,223
Mass (Da):136,487
Checksum:i2DB769E729F6D469
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451P → H in BAE27081 (PubMed:16141072).Curated
Sequence conflicti83 – 831V → G in BAE27081 (PubMed:16141072).Curated
Sequence conflicti142 – 1421Q → E in BAE27081 (PubMed:16141072).Curated
Sequence conflicti168 – 1681R → C in BAE27081 (PubMed:16141072).Curated
Sequence conflicti180 – 1801E → D in BAE27081 (PubMed:16141072).Curated
Sequence conflicti272 – 2721L → M in BAE27081 (PubMed:16141072).Curated
Sequence conflicti342 – 3421D → G in BAE27081 (PubMed:16141072).Curated
Sequence conflicti663 – 6631R → W in BAE27081 (PubMed:16141072).Curated
Sequence conflicti663 – 6631R → W in BAE32286 (PubMed:16141072).Curated
Sequence conflicti783 – 7831K → R in BAE27081 (PubMed:16141072).Curated
Sequence conflicti822 – 8221R → Q in BAE27081 (PubMed:16141072).Curated
Sequence conflicti991 – 9911Y → H in BAE27081 (PubMed:16141072).Curated
Sequence conflicti1211 – 12111M → V in AAH16202 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6464MVTPV…GTITR → MAAARRLMALAAGVSPRLRP PDPLVASGRQGCSRGFSSSF VRSDGTQEAAEVESEVAPSE PGEGDGSMVN in isoform 3. 1 PublicationVSP_019746Add
BLAST
Alternative sequencei1 – 2323Missing in isoform 2. 1 PublicationVSP_019747Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047557 mRNA. Translation: BAB32789.1.
AK146326 mRNA. Translation: BAE27081.1.
AK148778 mRNA. Translation: BAE28661.1.
AK153969 mRNA. Translation: BAE32286.1.
BC004082 mRNA. Translation: AAH04082.1.
BC016202 mRNA. Translation: AAH16202.1.
CCDSiCCDS23560.1. [Q99PU8-1]
CCDS57705.1. [Q99PU8-3]
CCDS57706.1. [Q99PU8-2]
RefSeqiNP_001239611.1. NM_001252682.1. [Q99PU8-2]
NP_001239612.1. NM_001252683.1. [Q99PU8-3]
NP_579925.1. NM_133347.2. [Q99PU8-1]
XP_006512361.1. XM_006512298.2. [Q99PU8-1]
XP_006512362.1. XM_006512299.1. [Q99PU8-1]
XP_006512363.1. XM_006512300.1. [Q99PU8-1]
XP_006512364.1. XM_006512301.2. [Q99PU8-1]
XP_006512366.1. XM_006512303.1. [Q99PU8-2]
XP_006512367.1. XM_006512304.1. [Q99PU8-2]
XP_006512368.1. XM_006512305.2. [Q99PU8-2]
XP_011241331.1. XM_011243029.1. [Q99PU8-1]
XP_011241332.1. XM_011243030.1. [Q99PU8-1]
XP_011241333.1. XM_011243031.1. [Q99PU8-2]
UniGeneiMm.276305.

Genome annotation databases

EnsembliENSMUST00000062368; ENSMUSP00000062622; ENSMUSG00000032480. [Q99PU8-3]
ENSMUST00000111991; ENSMUSP00000107622; ENSMUSG00000032480. [Q99PU8-2]
ENSMUST00000165596; ENSMUSP00000129174; ENSMUSG00000032480. [Q99PU8-1]
ENSMUST00000197928; ENSMUSP00000142549; ENSMUSG00000032480. [Q99PU8-2]
ENSMUST00000198425; ENSMUSP00000142659; ENSMUSG00000032480. [Q99PU8-1]
ENSMUST00000199529; ENSMUSP00000142489; ENSMUSG00000032480. [Q99PU8-2]
ENSMUST00000200066; ENSMUSP00000143371; ENSMUSG00000032480. [Q99PU8-2]
GeneIDi72831.
KEGGimmu:72831.
UCSCiuc009rtg.2. mouse. [Q99PU8-3]
uc009rth.2. mouse. [Q99PU8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047557 mRNA. Translation: BAB32789.1.
AK146326 mRNA. Translation: BAE27081.1.
AK148778 mRNA. Translation: BAE28661.1.
AK153969 mRNA. Translation: BAE32286.1.
BC004082 mRNA. Translation: AAH04082.1.
BC016202 mRNA. Translation: AAH16202.1.
CCDSiCCDS23560.1. [Q99PU8-1]
CCDS57705.1. [Q99PU8-3]
CCDS57706.1. [Q99PU8-2]
RefSeqiNP_001239611.1. NM_001252682.1. [Q99PU8-2]
NP_001239612.1. NM_001252683.1. [Q99PU8-3]
NP_579925.1. NM_133347.2. [Q99PU8-1]
XP_006512361.1. XM_006512298.2. [Q99PU8-1]
XP_006512362.1. XM_006512299.1. [Q99PU8-1]
XP_006512363.1. XM_006512300.1. [Q99PU8-1]
XP_006512364.1. XM_006512301.2. [Q99PU8-1]
XP_006512366.1. XM_006512303.1. [Q99PU8-2]
XP_006512367.1. XM_006512304.1. [Q99PU8-2]
XP_006512368.1. XM_006512305.2. [Q99PU8-2]
XP_011241331.1. XM_011243029.1. [Q99PU8-1]
XP_011241332.1. XM_011243030.1. [Q99PU8-1]
XP_011241333.1. XM_011243031.1. [Q99PU8-2]
UniGeneiMm.276305.

3D structure databases

ProteinModelPortaliQ99PU8.
SMRiQ99PU8. Positions 67-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215593. 1 interaction.
IntActiQ99PU8. 2 interactions.
MINTiMINT-1861298.
STRINGi10090.ENSMUSP00000062622.

PTM databases

iPTMnetiQ99PU8.
PhosphoSiteiQ99PU8.
SwissPalmiQ99PU8.

Proteomic databases

MaxQBiQ99PU8.
PaxDbiQ99PU8.
PeptideAtlasiQ99PU8.
PRIDEiQ99PU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062368; ENSMUSP00000062622; ENSMUSG00000032480. [Q99PU8-3]
ENSMUST00000111991; ENSMUSP00000107622; ENSMUSG00000032480. [Q99PU8-2]
ENSMUST00000165596; ENSMUSP00000129174; ENSMUSG00000032480. [Q99PU8-1]
ENSMUST00000197928; ENSMUSP00000142549; ENSMUSG00000032480. [Q99PU8-2]
ENSMUST00000198425; ENSMUSP00000142659; ENSMUSG00000032480. [Q99PU8-1]
ENSMUST00000199529; ENSMUSP00000142489; ENSMUSG00000032480. [Q99PU8-2]
ENSMUST00000200066; ENSMUSP00000143371; ENSMUSG00000032480. [Q99PU8-2]
GeneIDi72831.
KEGGimmu:72831.
UCSCiuc009rtg.2. mouse. [Q99PU8-3]
uc009rth.2. mouse. [Q99PU8-1]

Organism-specific databases

CTDi22907.
MGIiMGI:1920081. Dhx30.

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000112212.
HOVERGENiHBG081437.
InParanoidiQ99PU8.
KOiK13185.
OMAiPLTHAMY.
OrthoDBiEOG091G01RZ.
PhylomeDBiQ99PU8.
TreeFamiTF352030.

Miscellaneous databases

ChiTaRSiDhx30. mouse.
PROiQ99PU8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032480.
CleanExiMM_DHX30.
ExpressionAtlasiQ99PU8. baseline and differential.
GenevisibleiQ99PU8. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHX30_MOUSE
AccessioniPrimary (citable) accession number: Q99PU8
Secondary accession number(s): Q3U4Z4
, Q3UFA0, Q3UJS4, Q91WA7, Q99KN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.