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Protein

Ubiquitin carboxyl-terminal hydrolase BAP1

Gene

Bap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor (By similarity).By similarity

Miscellaneous

Has the ability to ability to suppress tumorigenicity when expressed in NCI-H226 cells.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91NucleophileCurated1
Active sitei169Proton donorBy similarity1
Sitei184Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Hydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5689603. UCH proteinases.
R-MMU-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Protein family/group databases

MEROPSiC12.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase BAP1 (EC:3.4.19.12)
Alternative name(s):
BRCA1-associated protein 1
Ubiquitin C-terminal hydrolase X4
Short name:
UCH-X4
Gene namesi
Name:Bap1
Synonyms:Kiaa0272
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1206586. Bap1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi91C → A: Abolishes ability to suppress tumorigenicity when expressed in NCI-H226 cells. 1 Publication1
Mutagenesisi716 – 721RRKRSR → AAAAAA: Abolishes ability to suppress tumorigenicity when expressed in NCI-H226 cells. 1 Publication6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002110701 – 728Ubiquitin carboxyl-terminal hydrolase BAP1Add BLAST728

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei292PhosphoserineBy similarity1
Modified residuei369PhosphoserineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei520PhosphoserineBy similarity1
Modified residuei536PhosphoserineBy similarity1
Modified residuei584PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) by UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99PU7.
MaxQBiQ99PU7.
PaxDbiQ99PU7.
PeptideAtlasiQ99PU7.
PRIDEiQ99PU7.

PTM databases

iPTMnetiQ99PU7.
PhosphoSitePlusiQ99PU7.

Expressioni

Tissue specificityi

Highly expressed in mammary glands, testis and ovary. Up-regulated in mammary glands during puberty, pregnancy, and as a result of parity.1 Publication

Gene expression databases

BgeeiENSMUSG00000021901.
CleanExiMM_BAP1.
ExpressionAtlasiQ99PU7. baseline and differential.
GenevisibleiQ99PU7. MM.

Interactioni

Subunit structurei

Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi222625. 28 interactors.
IntActiQ99PU7. 19 interactors.
STRINGi10090.ENSMUSP00000022458.

Structurei

3D structure databases

ProteinModelPortaliQ99PU7.
SMRiQ99PU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni595 – 720Interaction with BRCA1By similarityAdd BLAST126

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili629 – 660Sequence analysisAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi363 – 366HBM-like motifBy similarity4
Motifi716 – 721Nuclear localization signalBy similarity6

Sequence similaritiesi

Belongs to the peptidase C12 family. BAP1 subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2778. Eukaryota.
ENOG410XP0P. LUCA.
GeneTreeiENSGT00510000046560.
HOGENOMiHOG000013189.
HOVERGENiHBG054042.
InParanoidiQ99PU7.
KOiK08588.
OMAiTSHISKV.
OrthoDBiEOG091G08R7.
PhylomeDBiQ99PU7.
TreeFamiTF313976.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiView protein in InterPro
IPR001578. Peptidase_C12_UCH.
IPR036959. Peptidase_C12_UCH_sf.
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiView protein in Pfam
PF01088. Peptidase_C12. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

Q99PU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKGWLELES DPGLFTLLVE DFGVKGVQVE EIYDLQSKCQ GPVYGFIFLF
60 70 80 90 100
KWIEERRSRR KVSTLVDDTS VIDDDIVNNM FFAHQLIPNS CATHALLSVL
110 120 130 140 150
LNCSNVDLGP TLSRMKDFTK GFSPESKGYA IGNAPELAKA HNSHARPEPR
160 170 180 190 200
HLPEKQNGLS AVRTMEAFHF VSYVPITGRL FELDGLKVYP IDHGPWGEDE
210 220 230 240 250
EWTDKARRVI MERIGLATAG EPYHDIRFNL MAVVPDRRIK YETRLHVLKV
260 270 280 290 300
NRQTVLEALQ QLIRVTQPEL IQTHKSQESQ LPEESKPASS KSPLGLEAGR
310 320 330 340 350
TPVASECTQT DGAEEVAGSC PQTTTHSPPS KCKLVVKPPG SSLNGVPPNP
360 370 380 390 400
APIVQRLPAF LDNHNYAKSP MQEEEDLAAG VGRSRVPVRA PQQYSEDEDD
410 420 430 440 450
YEDEDEDVQN TNPAIRYKRK GTGKPGSLSN SSDGQLSVLQ PNTINVLTEK
460 470 480 490 500
LQESQKDLSV PLSIKTSSGA GSPAVAVPTH SQPSPTPSNE STDTASEIGS
510 520 530 540 550
AFNSPLRSPI RSANPTRPSS PVTSHISKVL FGEDDSLLRV DCIRYNRAVR
560 570 580 590 600
DLGPVISTGL LHLAEDGVLS PLALTEGGKG SSPSTRSSQG SQGSSGLEEK
610 620 630 640 650
EVVEVTESRD KPGLNRSSEP LSGEKYSPKE LLALLKCVEA EIANYEACLK
660 670 680 690 700
EEVEKRKKFK IDDQRRTHNY DEFICTFISM LAQEGMLANL VEQNISVRRR
710 720
QGVSIGRLHK QRKPDRRKRS RPYKAKRQ
Length:728
Mass (Da):80,492
Last modified:June 1, 2001 - v1
Checksum:i2BDEAFB09CD276DB
GO

Sequence cautioni

The sequence BAC97918 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti725A → V in BAC97918 (PubMed:14621295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047820 mRNA. Translation: BAB32976.1.
AK129108 mRNA. Translation: BAC97918.1. Different initiation.
AK170576 mRNA. Translation: BAE41889.1.
BC050901 mRNA. Translation: AAH50901.1.
CCDSiCCDS26910.1.
RefSeqiNP_081364.1. NM_027088.2.
UniGeneiMm.3779.

Genome annotation databases

EnsembliENSMUST00000022458; ENSMUSP00000022458; ENSMUSG00000021901.
GeneIDi104416.
KEGGimmu:104416.
UCSCiuc007sxh.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiBAP1_MOUSE
AccessioniPrimary (citable) accession number: Q99PU7
Secondary accession number(s): Q3TCR6, Q6ZQE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families