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Protein

Long-chain-fatty-acid--CoA ligase ACSBG1

Gene

Acsbg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids (By similarity).By similarity3 Publications

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei547ATPBy similarity1
Binding sitei562ATPBy similarity1
Binding sitei698ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi279 – 287ATPBy similarity9
Nucleotide bindingi469 – 474ATPBy similarity6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate--CoA ligase activity Source: UniProtKB-EC
  • long-chain fatty acid-CoA ligase activity Source: HGNC
  • very long-chain fatty acid-CoA ligase activity Source: HGNC

GO - Biological processi

  • long-chain fatty acid metabolic process Source: HGNC
  • response to glucocorticoid Source: MGI
  • very long-chain fatty acid metabolic process Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-75876. Synthesis of very long-chain fatty acyl-CoAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase ACSBG1 (EC:6.2.1.3)
Alternative name(s):
Acyl-CoA synthetase bubblegum family member 1
Short name:
mBG1
Gonadotropin-regulated long chain acyl CoA synthetase
Short name:
GR-LACS
Lipidosin
Gene namesi
Name:Acsbg1
Synonyms:Kiaa0631, Lpd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2385656. Acsbg1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi281G → A: Abolishes enzyme activity; when associated with M-287. 1 Publication1
Mutagenesisi287K → M: Abolishes enzyme activity; when associated with A-281. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003158101 – 721Long-chain-fatty-acid--CoA ligase ACSBG1Add BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineBy similarity1
Modified residuei50PhosphoserineBy similarity1
Modified residuei53PhosphoserineCombined sources1
Modified residuei70PhosphoserineCombined sources1
Modified residuei655PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99PU5.
PaxDbiQ99PU5.
PeptideAtlasiQ99PU5.
PRIDEiQ99PU5.

PTM databases

iPTMnetiQ99PU5.
PhosphoSitePlusiQ99PU5.
SwissPalmiQ99PU5.

Expressioni

Tissue specificityi

Mainly expressed in brain. Also expressed in adrenal gland and testis. In brain, it is present in cerebral cortical and cerebellar neurons and in steroidogenic cells of the adrenal gland, testis and ovary (at protein level).4 Publications

Developmental stagei

First detected on embryonic day 18 and increases steadily towards adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000032281.
CleanExiMM_ACSBG1.
ExpressionAtlasiQ99PU5. baseline and differential.
GenevisibleiQ99PU5. MM.

Interactioni

Protein-protein interaction databases

BioGridi220460. 1 interactor.
IntActiQ99PU5. 2 interactors.
STRINGi10090.ENSMUSP00000034822.

Structurei

3D structure databases

ProteinModelPortaliQ99PU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1256. Eukaryota.
COG1022. LUCA.
GeneTreeiENSGT00690000101725.
HOGENOMiHOG000230004.
HOVERGENiHBG054660.
InParanoidiQ99PU5.
KOiK15013.
OMAiTQQPNQC.
OrthoDBiEOG091G03FO.
PhylomeDBiQ99PU5.
TreeFamiTF354286.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99PU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGSEAGYC CLSRDSNMPD SRDDQQQGAS LGTSQDNSQT SSLIDGQTLS
60 70 80 90 100
KESPSHGLEL SAPEKARAAS LDGAEEALWT TRADGRVRLR LEPFCTQRPY
110 120 130 140 150
TVHQMFYEAL DKYGNLSALG FKRKDKWERI SYYQYYLIAR KVAKGFLKLG
160 170 180 190 200
LERAHSVAIL GFNSPEWFFS AVGTVFAGGI VTGIYTTSSP EACQYISHDC
210 220 230 240 250
RANVIVVDTQ KQLEKILKIW KDLPHLKAVV IYQEPPPKKM ANVYTMEELI
260 270 280 290 300
ELGQEVPEEA LDAIIDTQQP NQCCVLVYTS GTTGNPKGVM LSQDNITWTA
310 320 330 340 350
RYGSQAGDIQ PAEVQQEVVV SYLPLSHIAA QIYDLWTGIQ WGAQVCFADP
360 370 380 390 400
DALKGTLVNT LREVEPTSHM GVPRVWEKIM ERIQEVAAQS GFIRRKMLLW
410 420 430 440 450
AMSVTLEQNL TCPSNDLKPF TSRLADYLVL ARVRQALGFA KCQKNFYGAA
460 470 480 490 500
PMTAETQRFF LGLNIRLYAG YGLSESTGPH FMSSPYNYRL YSSGRVVPGC
510 520 530 540 550
RVKLVNQDAD GIGEICLWGR TIFMGYLNME DKTCEAIDSE GWLHTGDMGR
560 570 580 590 600
LDADGFLYIT GRLKELIITA GGENVPPVPI EEAVKMELPI ISSAMLIGDQ
610 620 630 640 650
RKFLSMLLTL KCTLDPETSE PTDSLTEQAV EFCQRVGSKA STVSEIVGQR
660 670 680 690 700
DEAVYQAIHE GIQRVNANAA ARPYHIQKWA ILQRDFSISG GELGPTMKLK
710 720
RLTVLEKYKD IIDSFYQEQK Q
Length:721
Mass (Da):80,426
Last modified:June 1, 2001 - v1
Checksum:iC3A8C91333E984FE
GO

Sequence cautioni

The sequence BAC97989 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299T → I in BAC97989 (PubMed:14621295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050554 mRNA. Translation: BAB32783.1.
DQ009026
, DQ009013, DQ009014, DQ009015, DQ009016, DQ009017, DQ009018, DQ009019, DQ009020, DQ009021, DQ009022, DQ009023, DQ009024, DQ009025 Genomic DNA. Translation: AAY58226.1.
AK129179 mRNA. Translation: BAC97989.1. Different initiation.
AK054103 mRNA. Translation: BAC35657.1.
BC057322 mRNA. Translation: AAH57322.1.
CCDSiCCDS23192.1.
PIRiJC7557.
RefSeqiNP_444408.1. NM_053178.2.
UniGeneiMm.20592.

Genome annotation databases

EnsembliENSMUST00000034822; ENSMUSP00000034822; ENSMUSG00000032281.
GeneIDi94180.
KEGGimmu:94180.
UCSCiuc009prj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050554 mRNA. Translation: BAB32783.1.
DQ009026
, DQ009013, DQ009014, DQ009015, DQ009016, DQ009017, DQ009018, DQ009019, DQ009020, DQ009021, DQ009022, DQ009023, DQ009024, DQ009025 Genomic DNA. Translation: AAY58226.1.
AK129179 mRNA. Translation: BAC97989.1. Different initiation.
AK054103 mRNA. Translation: BAC35657.1.
BC057322 mRNA. Translation: AAH57322.1.
CCDSiCCDS23192.1.
PIRiJC7557.
RefSeqiNP_444408.1. NM_053178.2.
UniGeneiMm.20592.

3D structure databases

ProteinModelPortaliQ99PU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220460. 1 interactor.
IntActiQ99PU5. 2 interactors.
STRINGi10090.ENSMUSP00000034822.

PTM databases

iPTMnetiQ99PU5.
PhosphoSitePlusiQ99PU5.
SwissPalmiQ99PU5.

Proteomic databases

MaxQBiQ99PU5.
PaxDbiQ99PU5.
PeptideAtlasiQ99PU5.
PRIDEiQ99PU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034822; ENSMUSP00000034822; ENSMUSG00000032281.
GeneIDi94180.
KEGGimmu:94180.
UCSCiuc009prj.1. mouse.

Organism-specific databases

CTDi23205.
MGIiMGI:2385656. Acsbg1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1256. Eukaryota.
COG1022. LUCA.
GeneTreeiENSGT00690000101725.
HOGENOMiHOG000230004.
HOVERGENiHBG054660.
InParanoidiQ99PU5.
KOiK15013.
OMAiTQQPNQC.
OrthoDBiEOG091G03FO.
PhylomeDBiQ99PU5.
TreeFamiTF354286.

Enzyme and pathway databases

ReactomeiR-MMU-75876. Synthesis of very long-chain fatty acyl-CoAs.

Miscellaneous databases

ChiTaRSiAcsbg1. mouse.
PROiQ99PU5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032281.
CleanExiMM_ACSBG1.
ExpressionAtlasiQ99PU5. baseline and differential.
GenevisibleiQ99PU5. MM.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACBG1_MOUSE
AccessioniPrimary (citable) accession number: Q99PU5
Secondary accession number(s): Q6ZQ79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.