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Protein

Rho GDP-dissociation inhibitor 1

Gene

Arhgdia

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-193634 Axonal growth inhibition (RHOA activation)
R-MMU-194840 Rho GTPase cycle
R-MMU-209563 Axonal growth stimulation

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GDP-dissociation inhibitor 1
Short name:
Rho GDI 1
Alternative name(s):
GDI-1
Rho-GDI alpha
Gene namesi
Name:Arhgdia
Synonyms:C87222, Gdi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2178103 Arhgdia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

From 1 week to 12 weeks of age, progressive deterioration from mild to massive albuminuria as consequence of renal abnormalities. In kidney, severe podocyte damage, glomerular lesions with focal and segmental sclerosis, along with prominent intratubular casts and luminal dilatation. Tubular epithelial cells show degenerative changes with basement membrane thickening. Increase of apoptotic cells in kidney glomeruli and tubulointerstitium.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi185Missing : Loss of interaction with CDC42, RHOA and RAC1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002190142 – 204Rho GDP-dissociation inhibitor 1Add BLAST203

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei34PhosphoserineCombined sources1
Modified residuei43N6-acetyllysineCombined sources1
Modified residuei47PhosphoserineBy similarity1
Modified residuei105N6-acetyllysineBy similarity1
Modified residuei127N6-acetyllysineBy similarity1
Cross-linki138Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki138Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei141N6-acetyllysine; alternateCombined sources1
Modified residuei141N6-succinyllysine; alternateCombined sources1
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei178N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99PT1
MaxQBiQ99PT1
PaxDbiQ99PT1
PeptideAtlasiQ99PT1
PRIDEiQ99PT1
TopDownProteomicsiQ99PT1

2D gel databases

REPRODUCTION-2DPAGEiIPI00322312
Q99PT1
UCD-2DPAGEiQ99PT1

PTM databases

iPTMnetiQ99PT1
PhosphoSitePlusiQ99PT1
SwissPalmiQ99PT1

Expressioni

Tissue specificityi

In kidney glomerulus, expressed in podocytes and mesangial cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000025132
CleanExiMM_ARHGDIA
GenevisibleiQ99PT1 MM

Interactioni

Subunit structurei

Monomer. Interacts with FER. Interacts with PLXNB3 (By similarity). Forms a heterodimer with RAC1. Interacts with RHOA, the affinity is increased by three orders of magnitude when RHOA is prenylated. Interacts with PSMD10; the interaction increases ARHGDIA association with RHOA, leading to ARHGDIA-mediated inactivation of RHOA and ROCK and prolonged AKT activation (By similarity). Interacts with RHOC and CDC42 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Rac3P607643EBI-494354,EBI-644949

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228703, 7 interactors
IntActiQ99PT1, 5 interactors
MINTiQ99PT1
STRINGi10090.ENSMUSP00000063714

Structurei

Secondary structure

1204
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 14Combined sources4
Helixi35 – 40Combined sources6
Turni41 – 44Combined sources4
Helixi46 – 56Combined sources11
Beta strandi69 – 78Combined sources10
Beta strandi86 – 92Combined sources7
Helixi94 – 99Combined sources6
Beta strandi101 – 105Combined sources5
Beta strandi109 – 118Combined sources10
Beta strandi123 – 134Combined sources12
Beta strandi137 – 149Combined sources13
Beta strandi152 – 156Combined sources5
Turni169 – 171Combined sources3
Beta strandi173 – 182Combined sources10
Beta strandi190 – 201Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F38X-ray2.80B2-204[»]
ProteinModelPortaliQ99PT1
SMRiQ99PT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Rho GDI family.Curated

Phylogenomic databases

eggNOGiKOG3205 Eukaryota
ENOG4111K44 LUCA
GeneTreeiENSGT00390000006233
HOGENOMiHOG000175765
HOVERGENiHBG000206
InParanoidiQ99PT1
KOiK12462
OMAiEGVEYQI
OrthoDBiEOG091G0MBU
PhylomeDBiQ99PT1
TreeFamiTF105387

Family and domain databases

Gene3Di2.70.50.30, 1 hit
InterProiView protein in InterPro
IPR014756 Ig_E-set
IPR000406 Rho_GDI
IPR024792 RhoGDI_dom_sf
PANTHERiPTHR10980 PTHR10980, 1 hit
PfamiView protein in Pfam
PF02115 Rho_GDI, 1 hit
PRINTSiPR00492 RHOGDI
SUPFAMiSSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQEPTAEQ LAQIAAENEE DEHSVNYKPP AQKSIQEIQE LDKDDESLRK
60 70 80 90 100
YKEALLGRVA VSADPNVPNV IVTRLTLVCS TAPGPLELDL TGDLESFKKQ
110 120 130 140 150
SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG
160 170 180 190 200
PRAEEYEFLT PMEEAPKGML ARGSYNIKSR FTDDDKTDHL SWEWNLTIKK

EWKD
Length:204
Mass (Da):23,407
Last modified:January 23, 2007 - v3
Checksum:i8ACE6F4456D842D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55L → P in AAH04732 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055070 mRNA Translation: BAB21527.1
AK075656 mRNA Translation: BAC35881.1
AK077343 mRNA Translation: BAC36761.1
AK143516 mRNA Translation: BAE25410.1
AK156819 mRNA Translation: BAE33865.1
AK165241 mRNA Translation: BAE38098.1
AK170553 mRNA Translation: BAE41876.1
AK170718 mRNA Translation: BAE41976.1
AK172140 mRNA Translation: BAE42844.1
AL663030 Genomic DNA No translation available.
BC004732 mRNA Translation: AAH04732.1
BC086755 mRNA Translation: AAH86755.1
CCDSiCCDS25743.1
RefSeqiNP_598557.3, NM_133796.7
XP_006532612.1, XM_006532549.3
UniGeneiMm.30016
Mm.456388
Mm.474783

Genome annotation databases

EnsembliENSMUST00000067936; ENSMUSP00000063714; ENSMUSG00000025132
ENSMUST00000106197; ENSMUSP00000101803; ENSMUSG00000025132
GeneIDi192662
KEGGimmu:192662
UCSCiuc007mtj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGDIR1_MOUSE
AccessioniPrimary (citable) accession number: Q99PT1
Secondary accession number(s): Q5M9P6, Q8BPI0, Q99KC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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