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Q99PR0 (POMT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein O-mannosyl-transferase 1
EC=2.4.1.109
Alternative name(s):
Dolichyl-phosphate-mannose--protein mannosyltransferase 1
Gene names
Name:Pomt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length747 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transfers mannosyl residues to the hydroxyl group of serine or threonine residues By similarity.

Catalytic activity

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

Cofactor

Magnesium By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the glycosyltransferase 39 family.

Contains 3 MIR domains.

Sequence caution

The sequence AAH70912.1 differs from that shown. Reason: Intron retention.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 747747Protein O-mannosyl-transferase 1
PRO_0000121486

Regions

Transmembrane30 – 5021Helical; Potential
Transmembrane90 – 11021Helical; Potential
Transmembrane121 – 14121Helical; Potential
Transmembrane176 – 19621Helical; Potential
Transmembrane205 – 22521Helical; Potential
Transmembrane228 – 24821Helical; Potential
Transmembrane267 – 28721Helical; Potential
Transmembrane597 – 61721Helical; Potential
Transmembrane636 – 65621Helical; Potential
Transmembrane660 – 68021Helical; Potential
Domain318 – 38164MIR 1
Domain392 – 44958MIR 2
Domain453 – 51361MIR 3

Amino acid modifications

Glycosylation4351N-linked (GlcNAc...) Potential
Glycosylation4711N-linked (GlcNAc...) Potential
Glycosylation5391N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q99PR0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 7ECE8F752E7FFA89

FASTA74785,496
        10         20         30         40         50         60 
MGNRSMGRED TLGVLPSLLF CKMLRFLKRP LVVTIDINLN LVALTVLGLL TRLWQLSYPR 

        70         80         90        100        110        120 
AVVFDEVYYG QYISFYMKRV FFLDDSGPPF GHMLLALGGW LGGFDGNFLW NRIGAEYSSN 

       130        140        150        160        170        180 
VPVWSLRLLP ALAGALSVPM AYQIVLELHF SHCTAMGAAL LMLIENALIT QSRLMLLESI 

       190        200        210        220        230        240 
LIFFNLLAVL SYLKFFNSQT HSPFSVHWWL WLMLTGVSCS CAVGIKYMGI FTYLLVLSIA 

       250        260        270        280        290        300 
AVHAWHLIGD QTLSNICVLS HLLARAVALL VVPVFLYLLF FYVHLMLLYR SGPHDQIMSS 

       310        320        330        340        350        360 
AFQASLEGGL ARITQGQPLE VAFGSQVTLK SVSGKPLPCW LHSHKNTYPM IYENGRGSSH 

       370        380        390        400        410        420 
QQQVTCYPFK DINNWWIVKD PGRHQLVVNN PPRPVRHGDI VQLVHGMTTR LLNTHDVAAP 

       430        440        450        460        470        480 
LSPHSQEVSC YIDYNISMPA QNLWKLDIVN RESNQDTWKT ILSEVRFVHV NTSAILKLSG 

       490        500        510        520        530        540 
AHLPDWGFRQ LEVVGEKLSL GPHESMVWNV EEHRYGRGHE QKERELELHS PTQHDISRNL 

       550        560        570        580        590        600 
SFMARFSELQ WKMLTLKNED LEHQYSSTPL EWLTLDTNIA YWLHPRTSAQ IHLLGNIVIW 

       610        620        630        640        650        660 
TSASLATVAY TLLFFWYLLR RRRNICDLPE DAWSHWVLAG ALCIGGWALN YLPFFLMERM 

       670        680        690        700        710        720 
LFLYHYLPAL TFQILLLPIV MQHASDHLCR SQLQRNVFSA LVVAWYSSAC HVSNMLRPLT 

       730        740 
YGDTSLSPGE LRALRWKDSW DILIRKY

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a rat homologue of the Saccharomyces protein O-mannosyltransferase gene."
Chiba A., Ronca F., Popp S., Margolis R.U.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF192388 mRNA. Translation: AAG53461.1.
BC070912 mRNA. Translation: AAH70912.1. Sequence problems.
IPIIPI00201298.
RefSeqNP_445858.1. NM_053406.2.
UniGeneRn.162659.

3D structure databases

ProteinModelPortalQ99PR0.
ModBaseSearch...

Protein family/group databases

CAZyGT39. Glycosyltransferase Family 39.

Proteomic databases

PRIDEQ99PR0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000015214; ENSRNOP00000015214; ENSRNOG00000010477.
GeneID84430.
KEGGrno:84430.
UCSCRGD:620078. rat.

Organism-specific databases

CTD10585.
RGD620078. Pomt1.

Phylogenomic databases

eggNOGCOG1928.
GeneTreeENSGT00700000104567.
HOGENOMHOG000157526.
HOVERGENHBG053637.
InParanoidQ99PR0.
KOK00728.
OMAPMAYQIV.
OrthoDBEOG47SSDF.

Enzyme and pathway databases

BRENDA2.4.1.109. 5301.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ99PR0.
GenevestigatorQ99PR0.
GermOnlineENSRNOG00000010477. Rattus norvegicus.

Family and domain databases

InterProIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERPTHR10050. PTHR10050. 1 hit.
PfamPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMSSF82109. MIR. 1 hit.
PROSITEPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio616936.

Entry information

Entry namePOMT1_RAT
AccessionPrimary (citable) accession number: Q99PR0
Secondary accession number(s): Q6IRI2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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