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Q99PP7

- TRI33_MOUSE

UniProt

Q99PP7 - TRI33_MOUSE

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Protein

E3 ubiquitin-protein ligase TRIM33

Gene

Trim33

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity).By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri141 – 20161RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri228 – 27548B box-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri287 – 32842B box-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri902 – 94948PHD-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. co-SMAD binding Source: BHF-UCL
  2. DNA binding Source: UniProtKB-KW
  3. ligase activity Source: UniProtKB-KW
  4. R-SMAD binding Source: BHF-UCL
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. negative regulation of BMP signaling pathway Source: UniProtKB
  2. protein ubiquitination Source: UniProtKB
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_220566. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM33 (EC:6.3.2.-)
Alternative name(s):
Ectodermin homolog
Transcription intermediary factor 1-gamma
Short name:
TIF1-gamma
Tripartite motif-containing protein 33
Gene namesi
Name:Trim33
Synonyms:Kiaa1113
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:2137357. Trim33.

Subcellular locationi

Nucleus 2 Publications
Note: In discrete nuclear dots resembling nuclear bodies.

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11421142E3 ubiquitin-protein ligase TRIM33PRO_0000056396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei778 – 7781N6-acetyllysine1 Publication
Modified residuei784 – 7841N6-acetyllysine1 Publication
Modified residuei808 – 8081N6-acetyllysine1 Publication
Modified residuei877 – 8771PhosphoserineBy similarity
Modified residuei966 – 9661N6-acetyllysine1 Publication
Modified residuei968 – 9681N6-acetyllysine1 Publication
Modified residuei1117 – 11171PhosphothreonineBy similarity
Modified residuei1120 – 11201PhosphoserineBy similarity

Post-translational modificationi

Sumoylated with SUMO1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ99PP7.
PaxDbiQ99PP7.
PRIDEiQ99PP7.

PTM databases

PhosphoSiteiQ99PP7.

Expressioni

Tissue specificityi

Ubiquitous with high level in testis.

Developmental stagei

Expressed in round hematopoietic cells in yolk sac blood islands at 8.5 dpc. Expressed uniformly at 10.5 dpc. Expressed in the brain, spinal cord, neuroepithelium and in spinal ganglia at 12.5 dpc. Expressed in brain, spinal cord, developing epithelia of the lung, stomach, intestine, outer region of the developing kidney, liver, brown fat tissue, skeletal muscle, developing craniofacial region, thymus, cochlear and pharyngeal epithelia and olfactory and respiratory epithelia at 16.5 dpc.2 Publications

Gene expression databases

BgeeiQ99PP7.
CleanExiMM_TRIM33.
ExpressionAtlasiQ99PP7. baseline and differential.
GenevestigatoriQ99PP7.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with TRIM24 and TRIM28 family members (By similarity). Interacts with SMAD4 in unstimulated cells (By similarity). Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta (By similarity). Interacts with SMAD2 and SMAD3 (By similarity). Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling (By similarity).By similarity

Protein-protein interaction databases

BioGridi220448. 5 interactions.
DIPiDIP-59674N.
IntActiQ99PP7. 3 interactions.
MINTiMINT-1867560.

Structurei

3D structure databases

ProteinModelPortaliQ99PP7.
SMRiQ99PP7. Positions 233-279, 286-330, 898-1101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini989 – 106173BromoPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 163163Necessary for E3 ubiquitin-protein ligase activity and repression of SMAD4 signaling and transcriptional repressionBy similarityAdd
BLAST
Regioni315 – 417103Necessary for oligomerizationBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili315 – 417103Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 186Poly-Gly
Compositional biasi20 – 122103Ala-richAdd
BLAST
Compositional biasi524 – 636113Gln-richAdd
BLAST
Compositional biasi708 – 78578Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri141 – 20161RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri228 – 27548B box-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri287 – 32842B box-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri902 – 94948PHD-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Bromodomain, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5076.
HOGENOMiHOG000252971.
HOVERGENiHBG054599.
InParanoidiQ99PP7.
KOiK08883.
PhylomeDBiQ99PP7.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProiIPR003649. Bbox_C.
IPR001487. Bromodomain.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000315. Znf_B-box.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00643. zf-B_box. 2 hits.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00297. BROMO. 1 hit.
SM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Alpha (identifier: Q99PP7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAENKGGGEA ESGGGGSGSA PVTAGAAGPT AQEAEPPLAA VLVEEEEEEG
60 70 80 90 100
GRAGAEGGAA GPDDGGVAAA SSSSAPAASV PAASVGSAVP GGAASTPAPA
110 120 130 140 150
AAPAPAPAPA PAPAPAPAPA PAPGSSSGPP LGPPASLLDT CAVCQQSLQS
160 170 180 190 200
RREAEPKLLP CLHSFCLRCL PEPERQLSVP IPGGSNGDVQ QVGVIRCPVC
210 220 230 240 250
RQECRQIDLV DNYFVKDTSE APSSSDEKSE QVCTSCEDNA SAVGFCVECG
260 270 280 290 300
EWLCKTCIEA HQRVKFTKDH LIRKKEDVSE SVGTSGQRPV FCPVHKQEQL
310 320 330 340 350
KLFCETCDRL TCRDCQLLEH KEHRYQFLEE AFQNQKGAIE NLLAKLLEKK
360 370 380 390 400
NYVHFAATQV QNRIKEVNET NKRVEQEIKV AIFTLINEIN KKGKSLLQQL
410 420 430 440 450
ENVTKERQMK LLQQQNDITG LSRQVKHVMN FTNWAIASGS STALLYSKRL
460 470 480 490 500
ITFQLRHILK ARCDPVPAAN GAIRFHCDPT FWAKNVVNLG NLVIESKPAP
510 520 530 540 550
GYTPNVVVGQ VPPGTNHISK TPGQINLAQL RLQHMQQQVY AQKHQQLQQM
560 570 580 590 600
RLQQPPAPIP TTTATTQQHP RQAAPQMLQQ QPPRLISVQT MQRGNMNCGA
610 620 630 640 650
FQAHQMRLAQ NAARIPGIPR HSAPQYSMMQ PHLQRQHSNP GHAGPFPVVS
660 670 680 690 700
AHNPINPTSP TTATMANANR GPTSPSVTAI ELIPSVTNPE NLPSLPDIPP
710 720 730 740 750
IQLEDAGSSS LDNLLSRYIS GSHLPPQPTS TMNPSPGPSA LSPGSSGLSN
760 770 780 790 800
SHTPVRPPST SSTGSRGSCG SSGRTAEKSA HSFKSDQVKV KQEPGTEEEI
810 820 830 840 850
CSFSGAVKQE KTEDGRRSAC MLSSPESSLT PPLSTNLHLE SELDTLTGLE
860 870 880 890 900
NHVKTEPTDI SESCKQSGLS NLVNGKSPIR NLMHRSARIG GDGNSKDDDP
910 920 930 940 950
NEDWCAVCQN GGDLLCCEKC PKVFHLTCHV PTLLSFPSGD WICTFCRDIG
960 970 980 990 1000
KPEVEYDCDN MQHSKKGKTA QGLSPVDQRK CERLLLYLYC HELSIEFQEP
1010 1020 1030 1040 1050
VPVSIPNYYK IIKKPMDLST VKKKLQKKHS QHYQIPDDFV ADVRLIFKNC
1060 1070 1080 1090 1100
ERFNEMMKVV QVYADTQEIN LKGDSEVAKA GKAVALYFED KLSEIYSDRT
1110 1120 1130 1140
FTPLPEFEQD EDDGEVTEDS DEDFIQPRRK RLKSDERPVH IK
Length:1,142
Mass (Da):123,843
Last modified:December 7, 2004 - v2
Checksum:iD43699AE75F0893F
GO
Isoform Beta (identifier: Q99PP7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1056-1072: Missing.

Show »
Length:1,125
Mass (Da):121,850
Checksum:iE19A5D1F2973E83D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti949 – 9524IGKP → HASA(PubMed:11331580)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1056 – 107217Missing in isoform Beta. 1 PublicationVSP_012068Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458590 mRNA. Translation: AAS10352.1.
AK129293 mRNA. Translation: BAC98103.1.
AF220138 mRNA. Translation: AAG53511.1.
RefSeqiNP_001073299.1. NM_001079830.2.
NP_444400.2. NM_053170.3.
UniGeneiMm.195036.

Genome annotation databases

GeneIDi94093.
KEGGimmu:94093.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458590 mRNA. Translation: AAS10352.1 .
AK129293 mRNA. Translation: BAC98103.1 .
AF220138 mRNA. Translation: AAG53511.1 .
RefSeqi NP_001073299.1. NM_001079830.2.
NP_444400.2. NM_053170.3.
UniGenei Mm.195036.

3D structure databases

ProteinModelPortali Q99PP7.
SMRi Q99PP7. Positions 233-279, 286-330, 898-1101.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 220448. 5 interactions.
DIPi DIP-59674N.
IntActi Q99PP7. 3 interactions.
MINTi MINT-1867560.

PTM databases

PhosphoSitei Q99PP7.

Proteomic databases

MaxQBi Q99PP7.
PaxDbi Q99PP7.
PRIDEi Q99PP7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 94093.
KEGGi mmu:94093.

Organism-specific databases

CTDi 51592.
MGIi MGI:2137357. Trim33.
Rougei Search...

Phylogenomic databases

eggNOGi COG5076.
HOGENOMi HOG000252971.
HOVERGENi HBG054599.
InParanoidi Q99PP7.
KOi K08883.
PhylomeDBi Q99PP7.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_220566. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

Miscellaneous databases

NextBioi 352085.
PROi Q99PP7.
SOURCEi Search...

Gene expression databases

Bgeei Q99PP7.
CleanExi MM_TRIM33.
ExpressionAtlasi Q99PP7. baseline and differential.
Genevestigatori Q99PP7.

Family and domain databases

Gene3Di 1.20.920.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProi IPR003649. Bbox_C.
IPR001487. Bromodomain.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000315. Znf_B-box.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
Pfami PF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00643. zf-B_box. 2 hits.
PF13639. zf-RING_2. 1 hit.
[Graphical view ]
PRINTSi PR00503. BROMODOMAIN.
SMARTi SM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00297. BROMO. 1 hit.
SM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
[Graphical view ]
SUPFAMi SSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEi PS50014. BROMODOMAIN_2. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, genomic structure, and expression analysis of the mouse transcriptional intermediary factor 1 gamma gene."
    Yan K.P., Dolle P., Mark M., Lerouge T., Wendling O., Chambon P., Losson R.
    Gene 334:3-13(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 55-1142 (ISOFORM BETA).
    Tissue: Embryonic tail.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 949-1142 (ISOFORM ALPHA).
  4. "The zebrafish moonshine gene encodes transcriptional intermediary factor 1 gamma, an essential regulator of hematopoiesis."
    Ransom D.G., Bahary N., Niss K., Traver D., Burns C., Trede N.S., Paffett-Lugassy N., Saganic W.J., Lim C.A., Hersey C., Zhou Y., Barut B.A., Lin S., Kingsley P.D., Palis J., Orkin S.H., Zon L.I.
    PLoS Biol. 2:1188-1196(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
  5. "Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway."
    He W., Dorn D.C., Erdjument-Bromage H., Tempst P., Moore M.A., Massague J.
    Cell 125:929-941(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  6. "In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice."
    Tirard M., Hsiao H.H., Nikolov M., Urlaub H., Melchior F., Brose N.
    Proc. Natl. Acad. Sci. U.S.A. 109:21122-21127(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION WITH SUMO1.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-778; LYS-784; LYS-808; LYS-966 AND LYS-968, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiTRI33_MOUSE
AccessioniPrimary (citable) accession number: Q99PP7
Secondary accession number(s): Q6SI71, Q6ZPX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: November 26, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3