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Protein

E3 ubiquitin-protein ligase TRIM33

Gene

Trim33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri141 – 201RING-typePROSITE-ProRule annotationAdd BLAST61
Zinc fingeri228 – 275B box-type 1PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri287 – 328B box-type 2PROSITE-ProRule annotationAdd BLAST42
Zinc fingeri902 – 949PHD-typePROSITE-ProRule annotationAdd BLAST48

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM33 (EC:6.3.2.-)
Alternative name(s):
Ectodermin homolog
Transcription intermediary factor 1-gamma
Short name:
TIF1-gamma
Tripartite motif-containing protein 33
Gene namesi
Name:Trim33
Synonyms:Kiaa1113
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2137357. Trim33.

Subcellular locationi

GO - Cellular componenti

  • intracellular membrane-bounded organelle Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563961 – 1142E3 ubiquitin-protein ligase TRIM33Add BLAST1142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki350Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei531Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei531Omega-N-methylarginine; alternateCombined sources1
Modified residuei551Omega-N-methylarginineBy similarity1
Modified residuei593Asymmetric dimethylarginineCombined sources1
Modified residuei607Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei607Omega-N-methylarginine; alternateBy similarity1
Modified residuei614Asymmetric dimethylarginineCombined sources1
Modified residuei620Asymmetric dimethylarginineCombined sources1
Modified residuei778N6-acetyllysine; alternateCombined sources1
Cross-linki778Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei784N6-acetyllysine; alternateCombined sources1
Cross-linki784Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki791Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki791Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei808N6-acetyllysine; alternateCombined sources1
Cross-linki808Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki808Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei818PhosphoserineCombined sources1
Modified residuei830PhosphothreonineCombined sources1
Cross-linki876Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei877PhosphoserineBy similarity1
Modified residuei966N6-acetyllysineCombined sources1
Modified residuei968N6-acetyllysine; alternateCombined sources1
Cross-linki968Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki1022Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1066PhosphothreonineBy similarity1
Cross-linki1072Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1117PhosphothreonineBy similarity1
Modified residuei1120PhosphoserineBy similarity1
Cross-linki1133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1134PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated with SUMO1.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99PP7.
PaxDbiQ99PP7.
PRIDEiQ99PP7.

PTM databases

iPTMnetiQ99PP7.
PhosphoSitePlusiQ99PP7.

Expressioni

Tissue specificityi

Ubiquitous with high level in testis.

Developmental stagei

Expressed in round hematopoietic cells in yolk sac blood islands at 8.5 dpc. Expressed uniformly at 10.5 dpc. Expressed in the brain, spinal cord, neuroepithelium and in spinal ganglia at 12.5 dpc. Expressed in brain, spinal cord, developing epithelia of the lung, stomach, intestine, outer region of the developing kidney, liver, brown fat tissue, skeletal muscle, developing craniofacial region, thymus, cochlear and pharyngeal epithelia and olfactory and respiratory epithelia at 16.5 dpc.2 Publications

Gene expression databases

BgeeiENSMUSG00000033014.
CleanExiMM_TRIM33.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with TRIM24 and TRIM28 family members (By similarity). Interacts with SMAD4 in unstimulated cells (By similarity). Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta (By similarity). Interacts with SMAD2 and SMAD3 (By similarity). Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220448. 5 interactors.
DIPiDIP-59674N.
IntActiQ99PP7. 3 interactors.
MINTiMINT-1867560.
STRINGi10090.ENSMUSP00000029444.

Structurei

3D structure databases

ProteinModelPortaliQ99PP7.
SMRiQ99PP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini989 – 1061BromoPROSITE-ProRule annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 163Necessary for E3 ubiquitin-protein ligase activity and repression of SMAD4 signaling and transcriptional repressionBy similarityAdd BLAST163
Regioni315 – 417Necessary for oligomerizationBy similarityAdd BLAST103

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili315 – 417Sequence analysisAdd BLAST103

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 18Poly-Gly6
Compositional biasi20 – 122Ala-richAdd BLAST103
Compositional biasi524 – 636Gln-richAdd BLAST113
Compositional biasi708 – 785Ser-richAdd BLAST78

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri141 – 201RING-typePROSITE-ProRule annotationAdd BLAST61
Zinc fingeri228 – 275B box-type 1PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri287 – 328B box-type 2PROSITE-ProRule annotationAdd BLAST42
Zinc fingeri902 – 949PHD-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Bromodomain, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFN. Eukaryota.
ENOG410Z421. LUCA.
HOGENOMiHOG000252971.
HOVERGENiHBG054599.
InParanoidiQ99PP7.
KOiK08883.
PhylomeDBiQ99PP7.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProiIPR003649. Bbox_C.
IPR001487. Bromodomain.
IPR019786. Zinc_finger_PHD-type_CS.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00297. BROMO. 1 hit.
SM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q99PP7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAENKGGGEA ESGGGGSGSA PVTAGAAGPT AQEAEPPLAA VLVEEEEEEG
60 70 80 90 100
GRAGAEGGAA GPDDGGVAAA SSSSAPAASV PAASVGSAVP GGAASTPAPA
110 120 130 140 150
AAPAPAPAPA PAPAPAPAPA PAPGSSSGPP LGPPASLLDT CAVCQQSLQS
160 170 180 190 200
RREAEPKLLP CLHSFCLRCL PEPERQLSVP IPGGSNGDVQ QVGVIRCPVC
210 220 230 240 250
RQECRQIDLV DNYFVKDTSE APSSSDEKSE QVCTSCEDNA SAVGFCVECG
260 270 280 290 300
EWLCKTCIEA HQRVKFTKDH LIRKKEDVSE SVGTSGQRPV FCPVHKQEQL
310 320 330 340 350
KLFCETCDRL TCRDCQLLEH KEHRYQFLEE AFQNQKGAIE NLLAKLLEKK
360 370 380 390 400
NYVHFAATQV QNRIKEVNET NKRVEQEIKV AIFTLINEIN KKGKSLLQQL
410 420 430 440 450
ENVTKERQMK LLQQQNDITG LSRQVKHVMN FTNWAIASGS STALLYSKRL
460 470 480 490 500
ITFQLRHILK ARCDPVPAAN GAIRFHCDPT FWAKNVVNLG NLVIESKPAP
510 520 530 540 550
GYTPNVVVGQ VPPGTNHISK TPGQINLAQL RLQHMQQQVY AQKHQQLQQM
560 570 580 590 600
RLQQPPAPIP TTTATTQQHP RQAAPQMLQQ QPPRLISVQT MQRGNMNCGA
610 620 630 640 650
FQAHQMRLAQ NAARIPGIPR HSAPQYSMMQ PHLQRQHSNP GHAGPFPVVS
660 670 680 690 700
AHNPINPTSP TTATMANANR GPTSPSVTAI ELIPSVTNPE NLPSLPDIPP
710 720 730 740 750
IQLEDAGSSS LDNLLSRYIS GSHLPPQPTS TMNPSPGPSA LSPGSSGLSN
760 770 780 790 800
SHTPVRPPST SSTGSRGSCG SSGRTAEKSA HSFKSDQVKV KQEPGTEEEI
810 820 830 840 850
CSFSGAVKQE KTEDGRRSAC MLSSPESSLT PPLSTNLHLE SELDTLTGLE
860 870 880 890 900
NHVKTEPTDI SESCKQSGLS NLVNGKSPIR NLMHRSARIG GDGNSKDDDP
910 920 930 940 950
NEDWCAVCQN GGDLLCCEKC PKVFHLTCHV PTLLSFPSGD WICTFCRDIG
960 970 980 990 1000
KPEVEYDCDN MQHSKKGKTA QGLSPVDQRK CERLLLYLYC HELSIEFQEP
1010 1020 1030 1040 1050
VPVSIPNYYK IIKKPMDLST VKKKLQKKHS QHYQIPDDFV ADVRLIFKNC
1060 1070 1080 1090 1100
ERFNEMMKVV QVYADTQEIN LKGDSEVAKA GKAVALYFED KLSEIYSDRT
1110 1120 1130 1140
FTPLPEFEQD EDDGEVTEDS DEDFIQPRRK RLKSDERPVH IK
Length:1,142
Mass (Da):123,843
Last modified:December 7, 2004 - v2
Checksum:iD43699AE75F0893F
GO
Isoform Beta (identifier: Q99PP7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1056-1072: Missing.

Show »
Length:1,125
Mass (Da):121,850
Checksum:iE19A5D1F2973E83D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti949 – 952IGKP → HASA (PubMed:11331580).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0120681056 – 1072Missing in isoform Beta. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458590 mRNA. Translation: AAS10352.1.
AK129293 mRNA. Translation: BAC98103.1.
AF220138 mRNA. Translation: AAG53511.1.
RefSeqiNP_001073299.1. NM_001079830.2.
NP_444400.2. NM_053170.3.
UniGeneiMm.195036.

Genome annotation databases

GeneIDi94093.
KEGGimmu:94093.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY458590 mRNA. Translation: AAS10352.1.
AK129293 mRNA. Translation: BAC98103.1.
AF220138 mRNA. Translation: AAG53511.1.
RefSeqiNP_001073299.1. NM_001079830.2.
NP_444400.2. NM_053170.3.
UniGeneiMm.195036.

3D structure databases

ProteinModelPortaliQ99PP7.
SMRiQ99PP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220448. 5 interactors.
DIPiDIP-59674N.
IntActiQ99PP7. 3 interactors.
MINTiMINT-1867560.
STRINGi10090.ENSMUSP00000029444.

PTM databases

iPTMnetiQ99PP7.
PhosphoSitePlusiQ99PP7.

Proteomic databases

EPDiQ99PP7.
PaxDbiQ99PP7.
PRIDEiQ99PP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi94093.
KEGGimmu:94093.

Organism-specific databases

CTDi51592.
MGIiMGI:2137357. Trim33.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410ITFN. Eukaryota.
ENOG410Z421. LUCA.
HOGENOMiHOG000252971.
HOVERGENiHBG054599.
InParanoidiQ99PP7.
KOiK08883.
PhylomeDBiQ99PP7.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ99PP7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033014.
CleanExiMM_TRIM33.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProiIPR003649. Bbox_C.
IPR001487. Bromodomain.
IPR019786. Zinc_finger_PHD-type_CS.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00297. BROMO. 1 hit.
SM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRI33_MOUSE
AccessioniPrimary (citable) accession number: Q99PP7
Secondary accession number(s): Q6SI71, Q6ZPX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.