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Q99PP7 (TRI33_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase TRIM33

EC=6.3.2.-
Alternative name(s):
Ectodermin homolog
Transcription intermediary factor 1-gamma
Short name=TIF1-gamma
Tripartite motif-containing protein 33
Gene names
Name:Trim33
Synonyms:Kiaa1113
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1142 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway By similarity. May act as a transcriptional repressor By similarity. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade By similarity. Plays a role in the control of cell proliferation By similarity. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) By similarity. Ref.5

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Homooligomer and heterooligomer with TRIM24 and TRIM28 family members By similarity. Interacts with SMAD4 in unstimulated cells By similarity. Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta By similarity. Interacts with SMAD2 and SMAD3 By similarity. Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling By similarity.

Subcellular location

Nucleus. Note: In discrete nuclear dots resembling nuclear bodies. Ref.4 Ref.5

Tissue specificity

Ubiquitous with high level in testis.

Developmental stage

Expressed in round hematopoietic cells in yolk sac blood islands at 8.5 dpc. Expressed uniformly at 10.5 dpc. Expressed in the brain, spinal cord, neuroepithelium and in spinal ganglia at 12.5 dpc. Expressed in brain, spinal cord, developing epithelia of the lung, stomach, intestine, outer region of the developing kidney, liver, brown fat tissue, skeletal muscle, developing craniofacial region, thymus, cochlear and pharyngeal epithelia and olfactory and respiratory epithelia at 16.5 dpc. Ref.4 Ref.5

Post-translational modification

Sumoylated with SUMO1. Ref.6

Sequence similarities

Belongs to the TRIM/RBCC family.

Contains 2 B box-type zinc fingers.

Contains 1 bromo domain.

Contains 1 PHD-type zinc finger.

Contains 1 RING-type zinc finger.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: Q99PP7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q99PP7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1056-1072: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11421142E3 ubiquitin-protein ligase TRIM33
PRO_0000056396

Regions

Domain989 – 106173Bromo
Zinc finger141 – 20161RING-type
Zinc finger228 – 27548B box-type 1
Zinc finger287 – 32842B box-type 2
Zinc finger902 – 94948PHD-type
Region1 – 163163Necessary for E3 ubiquitin-protein ligase activity and repression of SMAD4 signaling and transcriptional repression By similarity
Region315 – 417103Necessary for oligomerization By similarity
Coiled coil315 – 417103 Potential
Compositional bias13 – 186Poly-Gly
Compositional bias20 – 122103Ala-rich
Compositional bias524 – 636113Gln-rich
Compositional bias708 – 78578Ser-rich

Amino acid modifications

Modified residue7781N6-acetyllysine Ref.7
Modified residue7841N6-acetyllysine Ref.7
Modified residue8081N6-acetyllysine Ref.7
Modified residue8771Phosphoserine By similarity
Modified residue9661N6-acetyllysine Ref.7
Modified residue9681N6-acetyllysine Ref.7
Modified residue11171Phosphothreonine By similarity
Modified residue11201Phosphoserine By similarity

Natural variations

Alternative sequence1056 – 107217Missing in isoform Beta.
VSP_012068

Experimental info

Sequence conflict949 – 9524IGKP → HASA Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: D43699AE75F0893F

FASTA1,142123,843
        10         20         30         40         50         60 
MAENKGGGEA ESGGGGSGSA PVTAGAAGPT AQEAEPPLAA VLVEEEEEEG GRAGAEGGAA 

        70         80         90        100        110        120 
GPDDGGVAAA SSSSAPAASV PAASVGSAVP GGAASTPAPA AAPAPAPAPA PAPAPAPAPA 

       130        140        150        160        170        180 
PAPGSSSGPP LGPPASLLDT CAVCQQSLQS RREAEPKLLP CLHSFCLRCL PEPERQLSVP 

       190        200        210        220        230        240 
IPGGSNGDVQ QVGVIRCPVC RQECRQIDLV DNYFVKDTSE APSSSDEKSE QVCTSCEDNA 

       250        260        270        280        290        300 
SAVGFCVECG EWLCKTCIEA HQRVKFTKDH LIRKKEDVSE SVGTSGQRPV FCPVHKQEQL 

       310        320        330        340        350        360 
KLFCETCDRL TCRDCQLLEH KEHRYQFLEE AFQNQKGAIE NLLAKLLEKK NYVHFAATQV 

       370        380        390        400        410        420 
QNRIKEVNET NKRVEQEIKV AIFTLINEIN KKGKSLLQQL ENVTKERQMK LLQQQNDITG 

       430        440        450        460        470        480 
LSRQVKHVMN FTNWAIASGS STALLYSKRL ITFQLRHILK ARCDPVPAAN GAIRFHCDPT 

       490        500        510        520        530        540 
FWAKNVVNLG NLVIESKPAP GYTPNVVVGQ VPPGTNHISK TPGQINLAQL RLQHMQQQVY 

       550        560        570        580        590        600 
AQKHQQLQQM RLQQPPAPIP TTTATTQQHP RQAAPQMLQQ QPPRLISVQT MQRGNMNCGA 

       610        620        630        640        650        660 
FQAHQMRLAQ NAARIPGIPR HSAPQYSMMQ PHLQRQHSNP GHAGPFPVVS AHNPINPTSP 

       670        680        690        700        710        720 
TTATMANANR GPTSPSVTAI ELIPSVTNPE NLPSLPDIPP IQLEDAGSSS LDNLLSRYIS 

       730        740        750        760        770        780 
GSHLPPQPTS TMNPSPGPSA LSPGSSGLSN SHTPVRPPST SSTGSRGSCG SSGRTAEKSA 

       790        800        810        820        830        840 
HSFKSDQVKV KQEPGTEEEI CSFSGAVKQE KTEDGRRSAC MLSSPESSLT PPLSTNLHLE 

       850        860        870        880        890        900 
SELDTLTGLE NHVKTEPTDI SESCKQSGLS NLVNGKSPIR NLMHRSARIG GDGNSKDDDP 

       910        920        930        940        950        960 
NEDWCAVCQN GGDLLCCEKC PKVFHLTCHV PTLLSFPSGD WICTFCRDIG KPEVEYDCDN 

       970        980        990       1000       1010       1020 
MQHSKKGKTA QGLSPVDQRK CERLLLYLYC HELSIEFQEP VPVSIPNYYK IIKKPMDLST 

      1030       1040       1050       1060       1070       1080 
VKKKLQKKHS QHYQIPDDFV ADVRLIFKNC ERFNEMMKVV QVYADTQEIN LKGDSEVAKA 

      1090       1100       1110       1120       1130       1140 
GKAVALYFED KLSEIYSDRT FTPLPEFEQD EDDGEVTEDS DEDFIQPRRK RLKSDERPVH 


IK 

« Hide

Isoform Beta [UniParc].

Checksum: E19A5D1F2973E83D
Show »

FASTA1,125121,850

References

« Hide 'large scale' references
[1]"Molecular cloning, genomic structure, and expression analysis of the mouse transcriptional intermediary factor 1 gamma gene."
Yan K.P., Dolle P., Mark M., Lerouge T., Wendling O., Chambon P., Losson R.
Gene 334:3-13(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 55-1142 (ISOFORM BETA).
Tissue: Embryonic tail.
[3]"The tripartite motif family identifies cell compartments."
Reymond A., Meroni G., Fantozzi A., Merla G., Cairo S., Luzi L., Riganelli D., Zanaria E., Messali S., Cainarca S., Guffanti A., Minucci S., Pelicci P.G., Ballabio A.
EMBO J. 20:2140-2151(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 949-1142 (ISOFORM ALPHA).
[4]"The zebrafish moonshine gene encodes transcriptional intermediary factor 1 gamma, an essential regulator of hematopoiesis."
Ransom D.G., Bahary N., Niss K., Traver D., Burns C., Trede N.S., Paffett-Lugassy N., Saganic W.J., Lim C.A., Hersey C., Zhou Y., Barut B.A., Lin S., Kingsley P.D., Palis J., Orkin S.H., Zon L.I.
PLoS Biol. 2:1188-1196(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
[5]"Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway."
He W., Dorn D.C., Erdjument-Bromage H., Tempst P., Moore M.A., Massague J.
Cell 125:929-941(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[6]"In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice."
Tirard M., Hsiao H.H., Nikolov M., Urlaub H., Melchior F., Brose N.
Proc. Natl. Acad. Sci. U.S.A. 109:21122-21127(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION WITH SUMO1.
[7]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-778; LYS-784; LYS-808; LYS-966 AND LYS-968, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY458590 mRNA. Translation: AAS10352.1.
AK129293 mRNA. Translation: BAC98103.1.
AF220138 mRNA. Translation: AAG53511.1.
RefSeqNP_001073299.1. NM_001079830.2.
NP_444400.2. NM_053170.3.
UniGeneMm.195036.

3D structure databases

ProteinModelPortalQ99PP7.
SMRQ99PP7. Positions 139-205, 230-330, 898-1101.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid220448. 4 interactions.
DIPDIP-59674N.
IntActQ99PP7. 3 interactions.
MINTMINT-1867560.

PTM databases

PhosphoSiteQ99PP7.

Proteomic databases

PaxDbQ99PP7.
PRIDEQ99PP7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID94093.
KEGGmmu:94093.

Organism-specific databases

CTD51592.
MGIMGI:2137357. Trim33.
RougeSearch...

Phylogenomic databases

eggNOGCOG5076.
HOGENOMHOG000252971.
HOVERGENHBG054599.
InParanoidQ99PP7.
PhylomeDBQ99PP7.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ99PP7.
BgeeQ99PP7.
CleanExMM_TRIM33.
GenevestigatorQ99PP7.

Family and domain databases

Gene3D1.20.920.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProIPR003649. Bbox_C.
IPR001487. Bromodomain.
IPR019786. Zinc_finger_PHD-type_CS.
IPR000315. Znf_B-box.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamPF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00643. zf-B_box. 2 hits.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
PRINTSPR00503. BROMODOMAIN.
SMARTSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00297. BROMO. 1 hit.
SM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
SUPFAMSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEPS50014. BROMODOMAIN_2. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio352085.
PROQ99PP7.
SOURCESearch...

Entry information

Entry nameTRI33_MOUSE
AccessionPrimary (citable) accession number: Q99PP7
Secondary accession number(s): Q6SI71, Q6ZPX5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot