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Q99PP7

- TRI33_MOUSE

UniProt

Q99PP7 - TRI33_MOUSE

Protein

E3 ubiquitin-protein ligase TRIM33

Gene

Trim33

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway By similarity. May act as a transcriptional repressor By similarity. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade By similarity. Plays a role in the control of cell proliferation By similarity. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) By similarity.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri141 – 20161RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri228 – 27548B box-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri287 – 32842B box-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri902 – 94948PHD-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. co-SMAD binding Source: BHF-UCL
    2. DNA binding Source: UniProtKB-KW
    3. ligase activity Source: UniProtKB-KW
    4. R-SMAD binding Source: BHF-UCL
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. negative regulation of BMP signaling pathway Source: UniProtKB
    2. protein ubiquitination Source: UniProtKB
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_220566. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase TRIM33 (EC:6.3.2.-)
    Alternative name(s):
    Ectodermin homolog
    Transcription intermediary factor 1-gamma
    Short name:
    TIF1-gamma
    Tripartite motif-containing protein 33
    Gene namesi
    Name:Trim33
    Synonyms:Kiaa1113
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:2137357. Trim33.

    Subcellular locationi

    Nucleus 2 Publications
    Note: In discrete nuclear dots resembling nuclear bodies.

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11421142E3 ubiquitin-protein ligase TRIM33PRO_0000056396Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei778 – 7781N6-acetyllysine1 Publication
    Modified residuei784 – 7841N6-acetyllysine1 Publication
    Modified residuei808 – 8081N6-acetyllysine1 Publication
    Modified residuei877 – 8771PhosphoserineBy similarity
    Modified residuei966 – 9661N6-acetyllysine1 Publication
    Modified residuei968 – 9681N6-acetyllysine1 Publication
    Modified residuei1117 – 11171PhosphothreonineBy similarity
    Modified residuei1120 – 11201PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylated with SUMO1.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ99PP7.
    PaxDbiQ99PP7.
    PRIDEiQ99PP7.

    PTM databases

    PhosphoSiteiQ99PP7.

    Expressioni

    Tissue specificityi

    Ubiquitous with high level in testis.

    Developmental stagei

    Expressed in round hematopoietic cells in yolk sac blood islands at 8.5 dpc. Expressed uniformly at 10.5 dpc. Expressed in the brain, spinal cord, neuroepithelium and in spinal ganglia at 12.5 dpc. Expressed in brain, spinal cord, developing epithelia of the lung, stomach, intestine, outer region of the developing kidney, liver, brown fat tissue, skeletal muscle, developing craniofacial region, thymus, cochlear and pharyngeal epithelia and olfactory and respiratory epithelia at 16.5 dpc.2 Publications

    Gene expression databases

    ArrayExpressiQ99PP7.
    BgeeiQ99PP7.
    CleanExiMM_TRIM33.
    GenevestigatoriQ99PP7.

    Interactioni

    Subunit structurei

    Homooligomer and heterooligomer with TRIM24 and TRIM28 family members By similarity. Interacts with SMAD4 in unstimulated cells By similarity. Found in a complex with SMAD2 and SMAD3 upon addition of TGF-beta By similarity. Interacts with SMAD2 and SMAD3 By similarity. Interacts with SMAD4 under basal and induced conditions and, upon TGF-beta signaling, with activated SMAD2. Forms a ternary complex with SMAD4 and SMAD2 upon TGF-beta signaling By similarity.By similarity

    Protein-protein interaction databases

    BioGridi220448. 4 interactions.
    DIPiDIP-59674N.
    IntActiQ99PP7. 3 interactions.
    MINTiMINT-1867560.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99PP7.
    SMRiQ99PP7. Positions 139-207, 286-330, 898-1101.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini989 – 106173BromoPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 163163Necessary for E3 ubiquitin-protein ligase activity and repression of SMAD4 signaling and transcriptional repressionBy similarityAdd
    BLAST
    Regioni315 – 417103Necessary for oligomerizationBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili315 – 417103Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 186Poly-Gly
    Compositional biasi20 – 122103Ala-richAdd
    BLAST
    Compositional biasi524 – 636113Gln-richAdd
    BLAST
    Compositional biasi708 – 78578Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRIM/RBCC family.Curated
    Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 bromo domain.PROSITE-ProRule annotation
    Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri141 – 20161RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri228 – 27548B box-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri287 – 32842B box-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri902 – 94948PHD-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Bromodomain, Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5076.
    HOGENOMiHOG000252971.
    HOVERGENiHBG054599.
    InParanoidiQ99PP7.
    KOiK08883.
    PhylomeDBiQ99PP7.

    Family and domain databases

    Gene3Di1.20.920.10. 1 hit.
    3.30.40.10. 2 hits.
    4.10.45.10. 1 hit.
    InterProiIPR003649. Bbox_C.
    IPR001487. Bromodomain.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR000315. Znf_B-box.
    IPR011011. Znf_FYVE_PHD.
    IPR001965. Znf_PHD.
    IPR019787. Znf_PHD-finger.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF00439. Bromodomain. 1 hit.
    PF00628. PHD. 1 hit.
    PF00643. zf-B_box. 2 hits.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view]
    PRINTSiPR00503. BROMODOMAIN.
    SMARTiSM00502. BBC. 1 hit.
    SM00336. BBOX. 2 hits.
    SM00297. BROMO. 1 hit.
    SM00249. PHD. 2 hits.
    SM00184. RING. 2 hits.
    [Graphical view]
    SUPFAMiSSF47370. SSF47370. 1 hit.
    SSF57903. SSF57903. 1 hit.
    PROSITEiPS50014. BROMODOMAIN_2. 1 hit.
    PS50119. ZF_BBOX. 2 hits.
    PS01359. ZF_PHD_1. 1 hit.
    PS50016. ZF_PHD_2. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Alpha (identifier: Q99PP7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAENKGGGEA ESGGGGSGSA PVTAGAAGPT AQEAEPPLAA VLVEEEEEEG     50
    GRAGAEGGAA GPDDGGVAAA SSSSAPAASV PAASVGSAVP GGAASTPAPA 100
    AAPAPAPAPA PAPAPAPAPA PAPGSSSGPP LGPPASLLDT CAVCQQSLQS 150
    RREAEPKLLP CLHSFCLRCL PEPERQLSVP IPGGSNGDVQ QVGVIRCPVC 200
    RQECRQIDLV DNYFVKDTSE APSSSDEKSE QVCTSCEDNA SAVGFCVECG 250
    EWLCKTCIEA HQRVKFTKDH LIRKKEDVSE SVGTSGQRPV FCPVHKQEQL 300
    KLFCETCDRL TCRDCQLLEH KEHRYQFLEE AFQNQKGAIE NLLAKLLEKK 350
    NYVHFAATQV QNRIKEVNET NKRVEQEIKV AIFTLINEIN KKGKSLLQQL 400
    ENVTKERQMK LLQQQNDITG LSRQVKHVMN FTNWAIASGS STALLYSKRL 450
    ITFQLRHILK ARCDPVPAAN GAIRFHCDPT FWAKNVVNLG NLVIESKPAP 500
    GYTPNVVVGQ VPPGTNHISK TPGQINLAQL RLQHMQQQVY AQKHQQLQQM 550
    RLQQPPAPIP TTTATTQQHP RQAAPQMLQQ QPPRLISVQT MQRGNMNCGA 600
    FQAHQMRLAQ NAARIPGIPR HSAPQYSMMQ PHLQRQHSNP GHAGPFPVVS 650
    AHNPINPTSP TTATMANANR GPTSPSVTAI ELIPSVTNPE NLPSLPDIPP 700
    IQLEDAGSSS LDNLLSRYIS GSHLPPQPTS TMNPSPGPSA LSPGSSGLSN 750
    SHTPVRPPST SSTGSRGSCG SSGRTAEKSA HSFKSDQVKV KQEPGTEEEI 800
    CSFSGAVKQE KTEDGRRSAC MLSSPESSLT PPLSTNLHLE SELDTLTGLE 850
    NHVKTEPTDI SESCKQSGLS NLVNGKSPIR NLMHRSARIG GDGNSKDDDP 900
    NEDWCAVCQN GGDLLCCEKC PKVFHLTCHV PTLLSFPSGD WICTFCRDIG 950
    KPEVEYDCDN MQHSKKGKTA QGLSPVDQRK CERLLLYLYC HELSIEFQEP 1000
    VPVSIPNYYK IIKKPMDLST VKKKLQKKHS QHYQIPDDFV ADVRLIFKNC 1050
    ERFNEMMKVV QVYADTQEIN LKGDSEVAKA GKAVALYFED KLSEIYSDRT 1100
    FTPLPEFEQD EDDGEVTEDS DEDFIQPRRK RLKSDERPVH IK 1142
    Length:1,142
    Mass (Da):123,843
    Last modified:December 7, 2004 - v2
    Checksum:iD43699AE75F0893F
    GO
    Isoform Beta (identifier: Q99PP7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1056-1072: Missing.

    Show »
    Length:1,125
    Mass (Da):121,850
    Checksum:iE19A5D1F2973E83D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti949 – 9524IGKP → HASA(PubMed:11331580)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1056 – 107217Missing in isoform Beta. 1 PublicationVSP_012068Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY458590 mRNA. Translation: AAS10352.1.
    AK129293 mRNA. Translation: BAC98103.1.
    AF220138 mRNA. Translation: AAG53511.1.
    RefSeqiNP_001073299.1. NM_001079830.2.
    NP_444400.2. NM_053170.3.
    UniGeneiMm.195036.

    Genome annotation databases

    GeneIDi94093.
    KEGGimmu:94093.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY458590 mRNA. Translation: AAS10352.1 .
    AK129293 mRNA. Translation: BAC98103.1 .
    AF220138 mRNA. Translation: AAG53511.1 .
    RefSeqi NP_001073299.1. NM_001079830.2.
    NP_444400.2. NM_053170.3.
    UniGenei Mm.195036.

    3D structure databases

    ProteinModelPortali Q99PP7.
    SMRi Q99PP7. Positions 139-207, 286-330, 898-1101.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 220448. 4 interactions.
    DIPi DIP-59674N.
    IntActi Q99PP7. 3 interactions.
    MINTi MINT-1867560.

    PTM databases

    PhosphoSitei Q99PP7.

    Proteomic databases

    MaxQBi Q99PP7.
    PaxDbi Q99PP7.
    PRIDEi Q99PP7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 94093.
    KEGGi mmu:94093.

    Organism-specific databases

    CTDi 51592.
    MGIi MGI:2137357. Trim33.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5076.
    HOGENOMi HOG000252971.
    HOVERGENi HBG054599.
    InParanoidi Q99PP7.
    KOi K08883.
    PhylomeDBi Q99PP7.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_220566. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

    Miscellaneous databases

    NextBioi 352085.
    PROi Q99PP7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99PP7.
    Bgeei Q99PP7.
    CleanExi MM_TRIM33.
    Genevestigatori Q99PP7.

    Family and domain databases

    Gene3Di 1.20.920.10. 1 hit.
    3.30.40.10. 2 hits.
    4.10.45.10. 1 hit.
    InterProi IPR003649. Bbox_C.
    IPR001487. Bromodomain.
    IPR019786. Zinc_finger_PHD-type_CS.
    IPR000315. Znf_B-box.
    IPR011011. Znf_FYVE_PHD.
    IPR001965. Znf_PHD.
    IPR019787. Znf_PHD-finger.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    Pfami PF00439. Bromodomain. 1 hit.
    PF00628. PHD. 1 hit.
    PF00643. zf-B_box. 2 hits.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00503. BROMODOMAIN.
    SMARTi SM00502. BBC. 1 hit.
    SM00336. BBOX. 2 hits.
    SM00297. BROMO. 1 hit.
    SM00249. PHD. 2 hits.
    SM00184. RING. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47370. SSF47370. 1 hit.
    SSF57903. SSF57903. 1 hit.
    PROSITEi PS50014. BROMODOMAIN_2. 1 hit.
    PS50119. ZF_BBOX. 2 hits.
    PS01359. ZF_PHD_1. 1 hit.
    PS50016. ZF_PHD_2. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, genomic structure, and expression analysis of the mouse transcriptional intermediary factor 1 gamma gene."
      Yan K.P., Dolle P., Mark M., Lerouge T., Wendling O., Chambon P., Losson R.
      Gene 334:3-13(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 55-1142 (ISOFORM BETA).
      Tissue: Embryonic tail.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 949-1142 (ISOFORM ALPHA).
    4. "The zebrafish moonshine gene encodes transcriptional intermediary factor 1 gamma, an essential regulator of hematopoiesis."
      Ransom D.G., Bahary N., Niss K., Traver D., Burns C., Trede N.S., Paffett-Lugassy N., Saganic W.J., Lim C.A., Hersey C., Zhou Y., Barut B.A., Lin S., Kingsley P.D., Palis J., Orkin S.H., Zon L.I.
      PLoS Biol. 2:1188-1196(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
    5. "Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway."
      He W., Dorn D.C., Erdjument-Bromage H., Tempst P., Moore M.A., Massague J.
      Cell 125:929-941(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    6. "In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice."
      Tirard M., Hsiao H.H., Nikolov M., Urlaub H., Melchior F., Brose N.
      Proc. Natl. Acad. Sci. U.S.A. 109:21122-21127(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION WITH SUMO1.
    7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-778; LYS-784; LYS-808; LYS-966 AND LYS-968, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiTRI33_MOUSE
    AccessioniPrimary (citable) accession number: Q99PP7
    Secondary accession number(s): Q6SI71, Q6ZPX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3