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Q99PP2

- ZN318_MOUSE

UniProt

Q99PP2 - ZN318_MOUSE

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Protein

Zinc finger protein 318

Gene

Znf318

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Repressed AR-mediated transcriptional activation. May act as a transcriptional regulator during spermatogenesis and in particular, during meiotic division.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri912 – 94635Matrin-type 1Add
BLAST
Zinc fingeri985 – 100723Matrin-type 2Add
BLAST

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Meiosis, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 318
Alternative name(s):
Testicular zinc finger protein
Gene namesi
Name:Znf318
Synonyms:Tzf, Zfp318
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1889348. Zfp318.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 2064›2064Zinc finger protein 318PRO_0000191808Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphoserineBy similarity
Modified residuei73 – 731PhosphoserineBy similarity
Modified residuei324 – 3241PhosphoserineBy similarity
Modified residuei384 – 3841PhosphoserineBy similarity
Modified residuei886 – 8861PhosphoserineBy similarity
Modified residuei1272 – 12721PhosphoserineBy similarity
Modified residuei1735 – 17351PhosphoserineBy similarity
Modified residuei1881 – 18811PhosphoserineBy similarity
Modified residuei1967 – 19671PhosphoserineBy similarity
Modified residuei1970 – 19701PhosphoserineBy similarity
Modified residuei2021 – 20211PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99PP2.
PaxDbiQ99PP2.
PRIDEiQ99PP2.

PTM databases

PhosphoSiteiQ99PP2.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are highly expressed in testis, moderately expressed in adrenal gland and uterus and faintly expressed in brain, kidney and liver. Isoform 1 is expressed more in adrenal gland, uterus and liver than isoform 2 is. Expression during testicular development of isoform 1 and isoform 2 is restricted to spermatocytes at the pachytene stage of meiotic prophase and to round and elongated spermatids.2 Publications

Gene expression databases

CleanExiMM_ZFP318.
GenevestigatoriQ99PP2.

Interactioni

Subunit structurei

Interacts with the N-terminal domain of AR.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ99PP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili175 – 20329Sequence AnalysisAdd
BLAST
Coiled coili731 – 830100Sequence AnalysisAdd
BLAST
Coiled coili1625 – 165430Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1297 – 132630Pro-richAdd
BLAST

Sequence similaritiesi

Contains 2 matrin-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri912 – 94635Matrin-type 1Add
BLAST
Zinc fingeri985 – 100723Matrin-type 2Add
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG87509.
HOGENOMiHOG000043103.
HOVERGENiHBG107139.
InParanoidiQ99PP2.

Family and domain databases

InterProiIPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Fragment.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99PP2) [UniParc]FASTAAdd to Basket

Also known as: TZF-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
SLEESLRITV GNDHFCVSTP ERRRLSDRLG ISPVDGLQDM DRDDLTDDSD
60 70 80 90 100
FTRSSQCSRG LERYISREEG PLSPFLGQLD EDYRTRETFL HRPEFSPQSS
110 120 130 140 150
CHDELLRGTE RNRDKLKSSS YSIRSEERSR EAKRPRYDDT EKVHSSGGDH
160 170 180 190 200
SSFTSGTRNY RQRRSSPSPR FLDPEFRELD LARRKREEEE EQSRSLSQEL
210 220 230 240 250
VGVGDDQIGC SIPGLAGVLT TSEPGYSLQR PEEVPMMPKK SILKKRIEAD
260 270 280 290 300
MKPSLQLESF SSGASSGEDH PLYSEHSPLP LSGAIAAFTS EIENKGTTVE
310 320 330 340 350
ADLKEPQSNL YQWGPLREIP KDNSEKFDSF LGFKERLDLK AEGLEQQTDN
360 370 380 390 400
LLPHERASQD GSGFSRILSM LADPTITQEK RRRSFPDIED EEKFLYGDEE
410 420 430 440 450
EDIKSESPLK SLEDPESAGT RQKANSLPST PAVKLESLEE SNPEYAKIHN
460 470 480 490 500
LLKTIGLDIG VAEIGKLAAR TQERLHGKKP SSRPSADRRL SADRHLSGDR
510 520 530 540 550
HFSADRCSSV EHSFTADWRS SDPHRPESRE THHSNTQSPE VSHPHPASPV
560 570 580 590 600
DPYLRTKNSP PFLKSDHPVC HVSGPEVVGS GFQSSVAVRC MLPSAPSTPI
610 620 630 640 650
RLPHSAALSQ FHIPGASQFA AARIPPNYQG SVIPSASFDA YRHYMAYAAS
660 670 680 690 700
RWPMYPASQP PSHPLSDPHR LLPVTKQAAR SRPNLRVIPT VTPAKPKQEI
710 720 730 740 750
PVLGSISVKR IPVRVSIPSL IKYNPKKISD EKNRASQKQK VIEEREKLKT
760 770 780 790 800
EQEARQKKMF YLTTELERLH KQQGEMLRKK RREKDGHKDP LLMEVSRLQD
810 820 830 840 850
SIMKDIAELH KETEEAEKKQ SELDKVAQIL GIDIFDKSLK SSNDSKESTE
860 870 880 890 900
KPEKEKSKSP EKELSPSNSS SSNKESKMNE KSCIKSPSST ESLQPTVKQS
910 920 930 940 950
DQPVAAYEYY DAGSHWCKDC NTTCGTMFDF FTHMHNKKHT QTLDPYNRPW
960 970 980 990 1000
ASKTQSEAKQ DTVKRTDKIT VPAKGSEFLI PVTGFYCQLC EEFLGDPISG
1010 1020 1030 1040 1050
EQHVKGHQHN ENYKKYVEEN PLYEERRNLD RQAGLAVVLE TERRRQNELK
1060 1070 1080 1090 1100
RKLNEKPKEE KIEKKARIVR EVKEDDKAPG ELEEQLSEDG SAPEKGEVKG
1110 1120 1130 1140 1150
NASLRPQVKE EVKKEPSVAS IAASFGKFSW KKPEKEEEKG SVVTPGAPKE
1160 1170 1180 1190 1200
DTVETSKDRD DGKAEVGKAK PIKIKLSGKT VIAHTSPWTP VVTTSTQTKI
1210 1220 1230 1240 1250
RPNLPIPSTV LRKSGSATVS KPAPLNTFLS IKSSGTSTKP LPVVKESSSD
1260 1270 1280 1290 1300
LLLPPDIISK AFGGEEVVLK GSPEEKVELA EKNEPSQVPE QMLALLPPPP
1310 1320 1330 1340 1350
PPPPPPPPPP PPPPPQAVPQ LSAPSPAQAN VVLTPVKSNS VISQTFSLGF
1360 1370 1380 1390 1400
QGPNILNPGL PVAFMASEQP TVIPSDETAP GVSESDWDQT LISMLVRPPP
1410 1420 1430 1440 1450
PLSSVFSEQA KKLEKRNSCL ATANAKDLYD IFYSSGGKGA HETKLSSSTL
1460 1470 1480 1490 1500
ANGESSSLPR TESSDFSSTC TLNSSMSSED LPQCSALVTA TEISNLENPI
1510 1520 1530 1540 1550
SKGMESTGKW SVVDQIDPKS RDSTYSFLQP LTRLYQNKPY EIVSPKTDTL
1560 1570 1580 1590 1600
VMWTSGSSQN DTHKDRPPEG KIRFDLGEPG PPGTDSTSHL SDTHCQTNGP
1610 1620 1630 1640 1650
QKLIEINLID NQNKNQEVYQ SEGCRESEMK RKTELKGKVA TEEEEEEEEE
1660 1670 1680 1690 1700
GANSIEDSNS NHGNRNTWEG EIGQPKLSTV DKKGEQSSKL MTGHENTSKV
1710 1720 1730 1740 1750
VIELSPSLPS KRTKIDLFPS LLQNPKSMPE LLLLSPAGSG LCLKRQEIWE
1760 1770 1780 1790 1800
RPEKPGLEDV ELQGTRPELT VTIESKVLEN FDTTHLEVEG FASLRNLGDM
1810 1820 1830 1840 1850
HANFHNSQTE QTRRSPTALS EKMSEEISVS SVMCNPSSSS DIEPVPSFSG
1860 1870 1880 1890 1900
FPLESPKTLV LNFETEGAHS SSNSRNGRIT SNSLETGHPV ENVGHDLGGE
1910 1920 1930 1940 1950
RTHQALDLLA GGMLSEDVKE TSPLQKDLLR MESTTVSPSG LGPSPCLPDL
1960 1970 1980 1990 2000
VDFVTRTPGV PKQKPCSPLS EPDAFLKCSS LEMGSPPPEI LSVSVSEVAV
2010 2020 2030 2040 2050
PQVSEDNDSA LNLVKTPPSG SPSRDQVVGG NVSPREMPEQ EAAVDVIPDH
2060
TRSNVYNSQD YLNG
Length:2,064
Mass (Da):228,220
Last modified:January 15, 2008 - v2
Checksum:iCA64069FFA9D269B
GO
Isoform 2 (identifier: Q99PP2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     942-981: TLDPYNRPWA...PAKGSEFLIP → GQFQKSSHFQ...YGNNVRPCGQ
     982-2064: Missing.

Show »
Length:981
Mass (Da):110,832
Checksum:i8CCE812CE531C1F6
GO

Sequence cautioni

The sequence AAF61636.1 differs from that shown. Reason: Frameshift at positions 13 and 32.
The sequence AAK00650.1 differs from that shown. Reason: Frameshift at positions 13 and 32.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei942 – 98140TLDPY…EFLIP → GQFQKSSHFQTEGLKQMFLL QECRDRNHRDYGNNVRPCGQ in isoform 2. 1 PublicationVSP_016594Add
BLAST
Alternative sequencei982 – 20641083Missing in isoform 2. 1 PublicationVSP_016595Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF159455 mRNA. Translation: AAF61636.1. Frameshift.
AF227194 mRNA. Translation: AAK00650.1. Frameshift.
AK011661 mRNA. Translation: BAC25342.1.
AK157779 mRNA. Translation: BAE34193.1.
PIRiJC7316.
RefSeqiNP_997554.2. NM_207671.4.
UniGeneiMm.439916.

Genome annotation databases

GeneIDi57908.
KEGGimmu:57908.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF159455 mRNA. Translation: AAF61636.1 . Frameshift.
AF227194 mRNA. Translation: AAK00650.1 . Frameshift.
AK011661 mRNA. Translation: BAC25342.1 .
AK157779 mRNA. Translation: BAE34193.1 .
PIRi JC7316.
RefSeqi NP_997554.2. NM_207671.4.
UniGenei Mm.439916.

3D structure databases

ProteinModelPortali Q99PP2.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q99PP2.

Proteomic databases

MaxQBi Q99PP2.
PaxDbi Q99PP2.
PRIDEi Q99PP2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 57908.
KEGGi mmu:57908.

Organism-specific databases

CTDi 57908.
MGIi MGI:1889348. Zfp318.

Phylogenomic databases

eggNOGi NOG87509.
HOGENOMi HOG000043103.
HOVERGENi HBG107139.
InParanoidi Q99PP2.

Miscellaneous databases

ChiTaRSi ZNF318. mouse.
NextBioi 314073.
PROi Q99PP2.
SOURCEi Search...

Gene expression databases

CleanExi MM_ZFP318.
Genevestigatori Q99PP2.

Family and domain databases

InterProi IPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcript for a novel protein with a zinc finger motif is expressed at specific stages of mouse spermatogenesis."
    Inoue A., Ishiji A., Kasagi S., Ishizuka M., Hirose S., Baba T., Hagiwara H.
    Biochem. Biophys. Res. Commun. 273:398-403(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    Strain: ddY.
    Tissue: Testis.
  2. "Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis."
    Ishizuka M., Ohshima H., Tamura N., Nakada T., Inoue A., Hirose S., Hagiwara H.
    Biochem. Biophys. Res. Commun. 301:1079-1085(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: ddY.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 393-1057 AND 1999-2064 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. Cited for: FUNCTION, INTERACTION WITH AR.

Entry informationi

Entry nameiZN318_MOUSE
AccessioniPrimary (citable) accession number: Q99PP2
Secondary accession number(s): Q3TZL5, Q8BMX9, Q9JJ01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 15, 2008
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3