Q99PP2 (ZN318_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 318

Alternative name(s):
Testicular zinc finger protein

Gene names

Name:	Znf318
Synonyms:	Tzf, Zfp318

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2064 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Repressed AR-mediated transcriptional activation. May act as a transcriptional regulator during spermatogenesis and in particular, during meiotic division. Ref.1 Ref.2 Ref.4
Subunit structure	Interacts with the N-terminal domain of AR. Ref.4
Subcellular location	Nucleus Ref.2.
Tissue specificity	Isoform 1 and isoform 2 are highly expressed in testis, moderately expressed in adrenal gland and uterus and faintly expressed in brain, kidney and liver. Isoform 1 is expressed more in adrenal gland, uterus and liver than isoform 2 is. Expression during testicular development of isoform 1 and isoform 2 is restricted to spermatocytes at the pachytene stage of meiotic prophase and to round and elongated spermatids. Ref.1 Ref.2
Sequence similarities	Contains 2 matrin-type zinc fingers.
Sequence caution	The sequence AAF61636.1 differs from that shown. Reason: Frameshift at positions 13 and 32. The sequence AAK00650.1 differs from that shown. Reason: Frameshift at positions 13 and 32.

Ontologies

Keywords
Biological process	Meiosis Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiotic nuclear division Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-templated Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-templated Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.2. Source: MGI
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99PP2-1)

Also known as: TZF-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99PP2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     942-981: TLDPYNRPWA...PAKGSEFLIP → GQFQKSSHFQ...YGNNVRPCGQ
     982-2064: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 2064	›2064	Zinc finger protein 318		PRO_0000191808
Regions
	Zinc finger	912 – 946	35	Matrin-type 1
	Zinc finger	985 – 1007	23	Matrin-type 2
	Coiled coil	175 – 203	29	Potential
	Coiled coil	731 – 830	100	Potential
	Coiled coil	1625 – 1654	30	Potential
	Compositional bias	1297 – 1326	30	Pro-rich
Amino acid modifications
	Modified residue	32	1	Phosphoserine By similarity
	Modified residue	73	1	Phosphoserine By similarity
	Modified residue	324	1	Phosphoserine By similarity
	Modified residue	384	1	Phosphoserine By similarity
	Modified residue	886	1	Phosphoserine By similarity
	Modified residue	1272	1	Phosphoserine By similarity
	Modified residue	1735	1	Phosphoserine By similarity
	Modified residue	1881	1	Phosphoserine By similarity
	Modified residue	1967	1	Phosphoserine By similarity
	Modified residue	1970	1	Phosphoserine By similarity
	Modified residue	2021	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	942 – 981	40	TLDPY…EFLIP → GQFQKSSHFQTEGLKQMFLL QECRDRNHRDYGNNVRPCGQ in isoform 2.		VSP_016594
	Alternative sequence	982 – 2064	1083	Missing in isoform 2.		VSP_016595
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (TZF-L) [UniParc]. Last modified January 15, 2008. Version 2. Checksum: CA64069FFA9D269B	FASTA	2,064	228,220
	10 20 30 40 50 60 SLEESLRITV GNDHFCVSTP ERRRLSDRLG ISPVDGLQDM DRDDLTDDSD FTRSSQCSRG 70 80 90 100 110 120 LERYISREEG PLSPFLGQLD EDYRTRETFL HRPEFSPQSS CHDELLRGTE RNRDKLKSSS 130 140 150 160 170 180 YSIRSEERSR EAKRPRYDDT EKVHSSGGDH SSFTSGTRNY RQRRSSPSPR FLDPEFRELD 190 200 210 220 230 240 LARRKREEEE EQSRSLSQEL VGVGDDQIGC SIPGLAGVLT TSEPGYSLQR PEEVPMMPKK 250 260 270 280 290 300 SILKKRIEAD MKPSLQLESF SSGASSGEDH PLYSEHSPLP LSGAIAAFTS EIENKGTTVE 310 320 330 340 350 360 ADLKEPQSNL YQWGPLREIP KDNSEKFDSF LGFKERLDLK AEGLEQQTDN LLPHERASQD 370 380 390 400 410 420 GSGFSRILSM LADPTITQEK RRRSFPDIED EEKFLYGDEE EDIKSESPLK SLEDPESAGT 430 440 450 460 470 480 RQKANSLPST PAVKLESLEE SNPEYAKIHN LLKTIGLDIG VAEIGKLAAR TQERLHGKKP 490 500 510 520 530 540 SSRPSADRRL SADRHLSGDR HFSADRCSSV EHSFTADWRS SDPHRPESRE THHSNTQSPE 550 560 570 580 590 600 VSHPHPASPV DPYLRTKNSP PFLKSDHPVC HVSGPEVVGS GFQSSVAVRC MLPSAPSTPI 610 620 630 640 650 660 RLPHSAALSQ FHIPGASQFA AARIPPNYQG SVIPSASFDA YRHYMAYAAS RWPMYPASQP 670 680 690 700 710 720 PSHPLSDPHR LLPVTKQAAR SRPNLRVIPT VTPAKPKQEI PVLGSISVKR IPVRVSIPSL 730 740 750 760 770 780 IKYNPKKISD EKNRASQKQK VIEEREKLKT EQEARQKKMF YLTTELERLH KQQGEMLRKK 790 800 810 820 830 840 RREKDGHKDP LLMEVSRLQD SIMKDIAELH KETEEAEKKQ SELDKVAQIL GIDIFDKSLK 850 860 870 880 890 900 SSNDSKESTE KPEKEKSKSP EKELSPSNSS SSNKESKMNE KSCIKSPSST ESLQPTVKQS 910 920 930 940 950 960 DQPVAAYEYY DAGSHWCKDC NTTCGTMFDF FTHMHNKKHT QTLDPYNRPW ASKTQSEAKQ 970 980 990 1000 1010 1020 DTVKRTDKIT VPAKGSEFLI PVTGFYCQLC EEFLGDPISG EQHVKGHQHN ENYKKYVEEN 1030 1040 1050 1060 1070 1080 PLYEERRNLD RQAGLAVVLE TERRRQNELK RKLNEKPKEE KIEKKARIVR EVKEDDKAPG 1090 1100 1110 1120 1130 1140 ELEEQLSEDG SAPEKGEVKG NASLRPQVKE EVKKEPSVAS IAASFGKFSW KKPEKEEEKG 1150 1160 1170 1180 1190 1200 SVVTPGAPKE DTVETSKDRD DGKAEVGKAK PIKIKLSGKT VIAHTSPWTP VVTTSTQTKI 1210 1220 1230 1240 1250 1260 RPNLPIPSTV LRKSGSATVS KPAPLNTFLS IKSSGTSTKP LPVVKESSSD LLLPPDIISK 1270 1280 1290 1300 1310 1320 AFGGEEVVLK GSPEEKVELA EKNEPSQVPE QMLALLPPPP PPPPPPPPPP PPPPPQAVPQ 1330 1340 1350 1360 1370 1380 LSAPSPAQAN VVLTPVKSNS VISQTFSLGF QGPNILNPGL PVAFMASEQP TVIPSDETAP 1390 1400 1410 1420 1430 1440 GVSESDWDQT LISMLVRPPP PLSSVFSEQA KKLEKRNSCL ATANAKDLYD IFYSSGGKGA 1450 1460 1470 1480 1490 1500 HETKLSSSTL ANGESSSLPR TESSDFSSTC TLNSSMSSED LPQCSALVTA TEISNLENPI 1510 1520 1530 1540 1550 1560 SKGMESTGKW SVVDQIDPKS RDSTYSFLQP LTRLYQNKPY EIVSPKTDTL VMWTSGSSQN 1570 1580 1590 1600 1610 1620 DTHKDRPPEG KIRFDLGEPG PPGTDSTSHL SDTHCQTNGP QKLIEINLID NQNKNQEVYQ 1630 1640 1650 1660 1670 1680 SEGCRESEMK RKTELKGKVA TEEEEEEEEE GANSIEDSNS NHGNRNTWEG EIGQPKLSTV 1690 1700 1710 1720 1730 1740 DKKGEQSSKL MTGHENTSKV VIELSPSLPS KRTKIDLFPS LLQNPKSMPE LLLLSPAGSG 1750 1760 1770 1780 1790 1800 LCLKRQEIWE RPEKPGLEDV ELQGTRPELT VTIESKVLEN FDTTHLEVEG FASLRNLGDM 1810 1820 1830 1840 1850 1860 HANFHNSQTE QTRRSPTALS EKMSEEISVS SVMCNPSSSS DIEPVPSFSG FPLESPKTLV 1870 1880 1890 1900 1910 1920 LNFETEGAHS SSNSRNGRIT SNSLETGHPV ENVGHDLGGE RTHQALDLLA GGMLSEDVKE 1930 1940 1950 1960 1970 1980 TSPLQKDLLR MESTTVSPSG LGPSPCLPDL VDFVTRTPGV PKQKPCSPLS EPDAFLKCSS 1990 2000 2010 2020 2030 2040 LEMGSPPPEI LSVSVSEVAV PQVSEDNDSA LNLVKTPPSG SPSRDQVVGG NVSPREMPEQ 2050 2060 EAAVDVIPDH TRSNVYNSQD YLNG
	Isoform 2 [UniParc]. Checksum: 8CCE812CE531C1F6	FASTA	981	110,832
	10 20 30 40 50 60 SLEESLRITV GNDHFCVSTP ERRRLSDRLG ISPVDGLQDM DRDDLTDDSD FTRSSQCSRG 70 80 90 100 110 120 LERYISREEG PLSPFLGQLD EDYRTRETFL HRPEFSPQSS CHDELLRGTE RNRDKLKSSS 130 140 150 160 170 180 YSIRSEERSR EAKRPRYDDT EKVHSSGGDH SSFTSGTRNY RQRRSSPSPR FLDPEFRELD 190 200 210 220 230 240 LARRKREEEE EQSRSLSQEL VGVGDDQIGC SIPGLAGVLT TSEPGYSLQR PEEVPMMPKK 250 260 270 280 290 300 SILKKRIEAD MKPSLQLESF SSGASSGEDH PLYSEHSPLP LSGAIAAFTS EIENKGTTVE 310 320 330 340 350 360 ADLKEPQSNL YQWGPLREIP KDNSEKFDSF LGFKERLDLK AEGLEQQTDN LLPHERASQD 370 380 390 400 410 420 GSGFSRILSM LADPTITQEK RRRSFPDIED EEKFLYGDEE EDIKSESPLK SLEDPESAGT 430 440 450 460 470 480 RQKANSLPST PAVKLESLEE SNPEYAKIHN LLKTIGLDIG VAEIGKLAAR TQERLHGKKP 490 500 510 520 530 540 SSRPSADRRL SADRHLSGDR HFSADRCSSV EHSFTADWRS SDPHRPESRE THHSNTQSPE 550 560 570 580 590 600 VSHPHPASPV DPYLRTKNSP PFLKSDHPVC HVSGPEVVGS GFQSSVAVRC MLPSAPSTPI 610 620 630 640 650 660 RLPHSAALSQ FHIPGASQFA AARIPPNYQG SVIPSASFDA YRHYMAYAAS RWPMYPASQP 670 680 690 700 710 720 PSHPLSDPHR LLPVTKQAAR SRPNLRVIPT VTPAKPKQEI PVLGSISVKR IPVRVSIPSL 730 740 750 760 770 780 IKYNPKKISD EKNRASQKQK VIEEREKLKT EQEARQKKMF YLTTELERLH KQQGEMLRKK 790 800 810 820 830 840 RREKDGHKDP LLMEVSRLQD SIMKDIAELH KETEEAEKKQ SELDKVAQIL GIDIFDKSLK 850 860 870 880 890 900 SSNDSKESTE KPEKEKSKSP EKELSPSNSS SSNKESKMNE KSCIKSPSST ESLQPTVKQS 910 920 930 940 950 960 DQPVAAYEYY DAGSHWCKDC NTTCGTMFDF FTHMHNKKHT QGQFQKSSHF QTEGLKQMFL 970 980 LQECRDRNHR DYGNNVRPCG Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcript for a novel protein with a zinc finger motif is expressed at specific stages of mouse spermatogenesis." Inoue A., Ishiji A., Kasagi S., Ishizuka M., Hirose S., Baba T., Hagiwara H. Biochem. Biophys. Res. Commun. 273:398-403(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY. Strain: ddY. Tissue: Testis.
[2]	"Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis." Ishizuka M., Ohshima H., Tamura N., Nakada T., Inoue A., Hirose S., Hagiwara H. Biochem. Biophys. Res. Commun. 301:1079-1085(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: ddY.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 393-1057 AND 1999-2064 (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryo.
[4]	"A zinc finger protein TZF is a novel corepressor of androgen receptor." Ishizuka M., Kawate H., Takayanagi R., Ohshima H., Tao R.-H., Hagiwara H. Biochem. Biophys. Res. Commun. 331:1025-1031(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF159455 mRNA. Translation: AAF61636.1. Frameshift. AF227194 mRNA. Translation: AAK00650.1. Frameshift. AK011661 mRNA. Translation: BAC25342.1. AK157779 mRNA. Translation: BAE34193.1.
PIR	JC7316.
RefSeq	NP_997554.2. NM_207671.4.
UniGene	Mm.439916.
3D structure databases
ProteinModelPortal	Q99PP2.
ModBase	Search...
MobiDB	Search...
PTM databases
PhosphoSite	Q99PP2.
Proteomic databases
PaxDb	Q99PP2.
PRIDE	Q99PP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	57908.
KEGG	mmu:57908.
Organism-specific databases
CTD	57908.
MGI	MGI:1889348. Zfp318.
Phylogenomic databases
eggNOG	NOG87509.
HOGENOM	HOG000043103.
HOVERGEN	HBG107139.
InParanoid	Q99PP2.
Gene expression databases
CleanEx	MM_ZFP318.
Genevestigator	Q99PP2.
Family and domain databases
InterPro	IPR015880. Znf_C2H2-like. IPR003604. Znf_U1. [Graphical view]
SMART	SM00355. ZnF_C2H2. 2 hits. SM00451. ZnF_U1. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZNF318. mouse.
NextBio	314073.
PRO	Q99PP2.
SOURCE	Search...

Entry information

Entry name

ZN318_MOUSE

Accession

Primary (citable) accession number: Q99PP2
Secondary accession number(s): Q3TZL5, Q8BMX9, Q9JJ01

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 15, 2008
Last modified:	June 11, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program