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Protein

Zinc finger protein 318

Gene

Znf318

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 2: Acts as a transcriptional corepressor for AR-mediated transactivation function. May act as a transcriptional regulator during spermatogenesis and in particular, during meiotic division.3 Publications
Isoform 1: Acts as a transcriptional coactivator for AR-mediated transactivation function. May act as a transcriptional regulator during spermatogenesis and in particular, during meiotic division.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1085 – 1119Matrin-type 1Add BLAST35
Zinc fingeri1158 – 1180Matrin-type 2Add BLAST23

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • meiotic cell cycle Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Repressor
Biological processMeiosis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 318
Alternative name(s):
Testicular zinc finger protein
Gene namesi
Name:Znf318
Synonyms:Tzf, Zfp318
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componentsi: Chromosome 17, Unplaced

Organism-specific databases

MGIiMGI:1889348. Zfp318.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001918081 – 2237Zinc finger protein 318Add BLAST2237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei205PhosphoserineBy similarity1
Modified residuei237PhosphotyrosineCombined sources1
Modified residuei239PhosphoserineCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei497PhosphoserineBy similarity1
Modified residuei502PhosphoserineBy similarity1
Modified residuei531PhosphoserineBy similarity1
Modified residuei557PhosphoserineBy similarity1
Cross-linki577Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki583Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki596Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki607Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei865PhosphothreonineBy similarity1
Modified residuei1032PhosphoserineBy similarity1
Modified residuei1059PhosphoserineBy similarity1
Modified residuei1445PhosphoserineBy similarity1
Modified residuei1878PhosphoserineBy similarity1
Modified residuei1908PhosphoserineBy similarity1
Modified residuei1988PhosphoserineBy similarity1
Modified residuei2044PhosphoserineBy similarity1
Modified residuei2054PhosphoserineBy similarity1
Modified residuei2140PhosphoserineBy similarity1
Modified residuei2143PhosphoserineBy similarity1
Modified residuei2194PhosphoserineBy similarity1
Modified residuei2206PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiB0V2M3.
PaxDbiQ99PP2.
PeptideAtlasiQ99PP2.
PRIDEiQ99PP2.

PTM databases

iPTMnetiQ99PP2.
PhosphoSitePlusiQ99PP2.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are highly expressed in testis, moderately expressed in adrenal gland and uterus and faintly expressed in brain, kidney and liver. Isoform 1 is expressed more in adrenal gland, uterus and liver than isoform 2 is. Expression during testicular development of isoform 1 and isoform 2 is restricted to spermatocytes at the pachytene stage of meiotic prophase and to round and elongated spermatids.2 Publications

Gene expression databases

BgeeiENSMUSG00000015597.
CleanExiMM_ZFP318.
ExpressionAtlasiB0V2M3. baseline and differential.
GenevisibleiB0V2M3. MM.

Interactioni

Subunit structurei

Homodimer. Heterodimer of isoform 1 and isoform 2. Isoform 1 and isoform 2 interact with AR.2 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi208360. 1 interactor.
STRINGi10090.ENSMUSP00000109109.

Structurei

3D structure databases

ProteinModelPortaliQ99PP2.
SMRiQ99PP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 1114Interaction with AR1 PublicationAdd BLAST1114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili348 – 376Sequence analysisAdd BLAST29
Coiled coili904 – 1003Sequence analysisAdd BLAST100
Coiled coili1798 – 1827Sequence analysisAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1470 – 1499Pro-richAdd BLAST30

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1085 – 1119Matrin-type 1Add BLAST35
Zinc fingeri1158 – 1180Matrin-type 2Add BLAST23

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHGT. Eukaryota.
ENOG4111IJJ. LUCA.
GeneTreeiENSGT00390000000614.
HOGENOMiHOG000043103.
HOVERGENiHBG107139.
InParanoidiQ99PP2.
OMAiQHNEKYK.
OrthoDBiEOG091G00B9.
TreeFamiTF350583.

Family and domain databases

InterProiView protein in InterPro
IPR003604. Matrin/U1-like-C_Znf_C2H2.
SMARTiView protein in SMART
SM00451. ZnF_U1. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99PP2-1) [UniParc]FASTAAdd to basket
Also known as: TZF-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYRSGSRSSV SSHRSKDGSA SGPPPGRPVG ASSGPTRRPS SPPPPSCSSL
60 70 80 90 100
RLPARRHRSP SGHRGRWASP SPPRGRRGSP SPPRGRRASP SPTRGRRASP
110 120 130 140 150
SPPRGRRGSP SPPRARRGSP SPPRSRRHYP PGLGGFRGSI RGESRADFAR
160 170 180 190 200
DGRGDHPGGG GGSRRRSPGL CSDSSLEESL RITVGNDHFC VSTPERRRLS
210 220 230 240 250
DRLGSPVDGL QDMDRDDLTD DSVFTRSSQC SRGLERYISR EEGPLSPFLG
260 270 280 290 300
QLDEDYRTRE TFLHRPEFSP QSSCHDELLR GTERNRDKLK SSSYSIRSEE
310 320 330 340 350
RSREAKRPRY DDTEKVHSSG GDHSSFTSGT RNYRQRRSSP SPRFLDPEFR
360 370 380 390 400
ELDLARRKRE EEEEQSRSLS QELVGVGDDQ IGCSIPGLAG VLTTSEPGYS
410 420 430 440 450
LQRPEEVPMM PKKSILKKRI EADMKPSLQL ESFSSGASSG EDHPLYSEHS
460 470 480 490 500
PLPLSGAIAA FTSEIENKGT TVEADLKEPQ SNLYQWGPLR EIPKDNSEKF
510 520 530 540 550
DSFLGFKEKL DLKAEGLEQQ TDFLLPHERA SQDGSGFSRI LSMLADPTIT
560 570 580 590 600
QEKRRRSFPD IEDEEKFLYG DEEEDIKSES PLKSLEDPES AGTRQKANSL
610 620 630 640 650
PSTPAVKLES LEESNPEYAK IHNLLKTIGL DIGVAEIGKL AARTQERLHG
660 670 680 690 700
KKPSSRPSAD RRLSADRHLS GDRHFSADRC SSVEHSFTAD WRSSDPHRPE
710 720 730 740 750
SRETHHSNTQ SPEVSHPHPA SPVDPYLRTK NSPPFLKSDH PVCHVSGPEV
760 770 780 790 800
VGSGFQSSVA VRCMLPSAPS TPIRLPHSAA LSQFHIPGAS QFAAARIPPN
810 820 830 840 850
YQGSVIPSAS FDAYRHYMAY AASRWPMYPA SQPPSHPLSD PHRLLPVTKQ
860 870 880 890 900
AARSRPNLRV IPTVTPAKPK QEIPVLGSIS VKRIPVRVSI PSLIKYNPKK
910 920 930 940 950
ISDEKNRASQ KQKVIEEREK LKTEQEARQK KMFYLTTELE RLHKQQGEML
960 970 980 990 1000
RKKRREKDGH KDPLLMEVSR LQDSIMKDIA ELHKETEEAE KKQSELDKVA
1010 1020 1030 1040 1050
QILGIDIFDK SLKSSNDSKE STEKPEKEKS KSPEKELSPS NSSSSNKESK
1060 1070 1080 1090 1100
MNEKSCIKSP SSTESLQPTV KQSDQPVAAY EYYDAGSHWC KDCNTTCGTM
1110 1120 1130 1140 1150
FDFFTHMHNK KHTQTLDPYN RPWASKTQSE AKQDTVKRTD KITVPAKGSE
1160 1170 1180 1190 1200
FLIPVTGFYC QLCEEFLGDP ISGEQHVKGH QHNENYKKYV EENPLYEERR
1210 1220 1230 1240 1250
NLDRQAGLAV VLETERRRQN ELKRKLNEKP KEEKIEKKAR IVREVKEDDK
1260 1270 1280 1290 1300
APGELEEQLS EDGSAPEKGE VKGNASLRPQ VKEEVKKEPS VASIAASFGK
1310 1320 1330 1340 1350
FSWKKPEKEE EKGSVVTPGA PKEDTVETSK DRDDGKAEVG KAKPIKIKLS
1360 1370 1380 1390 1400
GKTVIAHTSP WTPVVTTSTQ TKIRPNLPIP STVLRKSGSA TVSKPAPLNT
1410 1420 1430 1440 1450
FLSIKSSGTS TKPLPVVKES SSDLLLPPDI ISKAFGGEEV VLKGSPEEKV
1460 1470 1480 1490 1500
ELAEKNEPSQ VPEQMLALLP PPPPPPPPPP PPPPPPPPQA VPQLSAPSPA
1510 1520 1530 1540 1550
QANVVLTPVK SNSVISQTFS LGFQGPNILN PGLPVAFMAS EQPTVIPSDE
1560 1570 1580 1590 1600
TAPGVSESDW DQTLISMLVR PPPPLSSVFS EQAKKLEKRN SCLATANAKD
1610 1620 1630 1640 1650
LYDIFYSSGG KGAHETKLSS STLANGESSS LPRTESSDFS STCTLNSSMS
1660 1670 1680 1690 1700
SEDLPQCSAL VTATEISNLE NPISKGMEST GKWSVVDQID PKSRDSTYSF
1710 1720 1730 1740 1750
LQPLTRLYQN KPYEIVSPKT DTLVMWTSGS SQNDTHKDRP PEGKIRFDLG
1760 1770 1780 1790 1800
EPGPPGTDST SHLSDTHCQT NGPQKLIEIN LIDNQNKNQE VYQSEGCRES
1810 1820 1830 1840 1850
EMKRKTELKG KVATEEEEEE EEEGANSIED SNSNHGNRNT WEGEIGQPKL
1860 1870 1880 1890 1900
STVDKKGEQS SKLMTGHENT SKVVIELSPS LPSKRTKIDL FPSLLQNPKS
1910 1920 1930 1940 1950
MPELLLLSPA GSGLCLKRQE IWERPEKPGL EDVELQGTRP ELTVTIESKV
1960 1970 1980 1990 2000
LENFDTTHLE VEGFASLRNL GDMHANFHNS QTEQTRRSPT ALSEKMSEEI
2010 2020 2030 2040 2050
SVSSVMCNPS SSSDIEPVPS FSGFPLESPK TLVLNFETEG AHSSSNSRNG
2060 2070 2080 2090 2100
RITSNSLETG HPVENVGHDL GGERTHQALD LLAGGMLSED VKETSPLQKD
2110 2120 2130 2140 2150
LLRMESTTVS PSGLGPSPCL PDLVDFVTRT PGVPKQKPCS PLSEPDAFLK
2160 2170 2180 2190 2200
CSSLEMGSPP PEILSVSVSE VAVPQVSEDN DSALNLVKTP PSGSPSRDQV
2210 2220 2230
VGGNVSPREM PEQEAAVDVI PDHTRSNVYN SQDYLNG
Length:2,237
Mass (Da):246,324
Last modified:January 31, 2018 - v3
Checksum:i97B600577866BA14
GO
Isoform 2 (identifier: Q99PP2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1154: TLDPYNRPWA...PAKGSEFLIP → GQFQKSSHFQ...YGNNVRPCGQ
     1155-2237: Missing.

Show »
Length:1,154
Mass (Da):128,936
Checksum:i337662D042648F18
GO

Sequence cautioni

The sequence AAF61636 differs from that shown. Reason: Frameshift at positions 187 and 205.Curated
The sequence AAF61636 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI50731 differs from that shown. Reason: Frameshift at position 205.Curated
The sequence AAI50731 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAK00650 differs from that shown. Reason: Frameshift at positions 187 and 205.Curated
The sequence AAK00650 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223V → D in AAF61636 (PubMed:10873617).Curated1
Sequence conflicti223V → D in AAK00650 (PubMed:12589823).Curated1
Sequence conflicti295S → F in AAI50731 (PubMed:15489334).Curated1
Sequence conflicti509K → R in AAF61636 (PubMed:10873617).Curated1
Sequence conflicti509K → R in AAK00650 (PubMed:12589823).Curated1
Sequence conflicti523F → N in AAF61636 (PubMed:10873617).Curated1
Sequence conflicti523F → N in AAK00650 (PubMed:12589823).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0165941115 – 1154TLDPY…EFLIP → GQFQKSSHFQTEGLKQMFLL QECRDRNHRDYGNNVRPCGQ in isoform 2. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_0165951155 – 2237Missing in isoform 2. 1 PublicationAdd BLAST1083

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC151275 Genomic DNA. No translation available.
CT009572 Genomic DNA. No translation available.
AF159455 mRNA. Translation: AAF61636.1. Sequence problems.
AF227194 mRNA. Translation: AAK00650.1. Sequence problems.
BC150730 mRNA. Translation: AAI50731.1. Sequence problems.
AK011661 mRNA. Translation: BAC25342.1.
AK157779 mRNA. Translation: BAE34193.1.
PIRiJC7316.
RefSeqiNP_997554.2. NM_207671.4.
UniGeneiMm.439916.

Genome annotation databases

EnsembliENSMUST00000113481; ENSMUSP00000109109; ENSMUSG00000015597.
GeneIDi57908.
KEGGimmu:57908.
UCSCiuc008csq.3. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZN318_MOUSE
AccessioniPrimary (citable) accession number: Q99PP2
Secondary accession number(s): B0V2M3
, B9EK88, Q3TZL5, Q8BMX9, Q9JJ01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 31, 2018
Last modified: January 31, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot