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Q99PP2 (ZN318_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 318
Alternative name(s):
Testicular zinc finger protein
Gene names
Name:Znf318
Synonyms:Tzf, Zfp318
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2064 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Repressed AR-mediated transcriptional activation. May act as a transcriptional regulator during spermatogenesis and in particular, during meiotic division. Ref.1 Ref.2 Ref.4

Subunit structure

Interacts with the N-terminal domain of AR. Ref.4

Subcellular location

Nucleus Ref.2.

Tissue specificity

Isoform 1 and isoform 2 are highly expressed in testis, moderately expressed in adrenal gland and uterus and faintly expressed in brain, kidney and liver. Isoform 1 is expressed more in adrenal gland, uterus and liver than isoform 2 is. Expression during testicular development of isoform 1 and isoform 2 is restricted to spermatocytes at the pachytene stage of meiotic prophase and to round and elongated spermatids. Ref.1 Ref.2

Sequence similarities

Contains 2 matrin-type zinc fingers.

Sequence caution

The sequence AAF61636.1 differs from that shown. Reason: Frameshift at positions 13 and 32.

The sequence AAK00650.1 differs from that shown. Reason: Frameshift at positions 13 and 32.

Ontologies

Keywords
   Biological processMeiosis
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionRepressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmeiotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay Ref.2. Source: MGI

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99PP2-1)

Also known as: TZF-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99PP2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     942-981: TLDPYNRPWA...PAKGSEFLIP → GQFQKSSHFQ...YGNNVRPCGQ
     982-2064: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 2064›2064Zinc finger protein 318
PRO_0000191808

Regions

Zinc finger912 – 94635Matrin-type 1
Zinc finger985 – 100723Matrin-type 2
Coiled coil175 – 20329 Potential
Coiled coil731 – 830100 Potential
Coiled coil1625 – 165430 Potential
Compositional bias1297 – 132630Pro-rich

Amino acid modifications

Modified residue321Phosphoserine By similarity
Modified residue731Phosphoserine By similarity
Modified residue3241Phosphoserine By similarity
Modified residue3841Phosphoserine By similarity
Modified residue8861Phosphoserine By similarity
Modified residue12721Phosphoserine By similarity
Modified residue17351Phosphoserine By similarity
Modified residue18811Phosphoserine By similarity
Modified residue19671Phosphoserine By similarity
Modified residue19701Phosphoserine By similarity
Modified residue20211Phosphoserine By similarity

Natural variations

Alternative sequence942 – 98140TLDPY…EFLIP → GQFQKSSHFQTEGLKQMFLL QECRDRNHRDYGNNVRPCGQ in isoform 2.
VSP_016594
Alternative sequence982 – 20641083Missing in isoform 2.
VSP_016595

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (TZF-L) [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: CA64069FFA9D269B

FASTA2,064228,220
        10         20         30         40         50         60 
SLEESLRITV GNDHFCVSTP ERRRLSDRLG ISPVDGLQDM DRDDLTDDSD FTRSSQCSRG 

        70         80         90        100        110        120 
LERYISREEG PLSPFLGQLD EDYRTRETFL HRPEFSPQSS CHDELLRGTE RNRDKLKSSS 

       130        140        150        160        170        180 
YSIRSEERSR EAKRPRYDDT EKVHSSGGDH SSFTSGTRNY RQRRSSPSPR FLDPEFRELD 

       190        200        210        220        230        240 
LARRKREEEE EQSRSLSQEL VGVGDDQIGC SIPGLAGVLT TSEPGYSLQR PEEVPMMPKK 

       250        260        270        280        290        300 
SILKKRIEAD MKPSLQLESF SSGASSGEDH PLYSEHSPLP LSGAIAAFTS EIENKGTTVE 

       310        320        330        340        350        360 
ADLKEPQSNL YQWGPLREIP KDNSEKFDSF LGFKERLDLK AEGLEQQTDN LLPHERASQD 

       370        380        390        400        410        420 
GSGFSRILSM LADPTITQEK RRRSFPDIED EEKFLYGDEE EDIKSESPLK SLEDPESAGT 

       430        440        450        460        470        480 
RQKANSLPST PAVKLESLEE SNPEYAKIHN LLKTIGLDIG VAEIGKLAAR TQERLHGKKP 

       490        500        510        520        530        540 
SSRPSADRRL SADRHLSGDR HFSADRCSSV EHSFTADWRS SDPHRPESRE THHSNTQSPE 

       550        560        570        580        590        600 
VSHPHPASPV DPYLRTKNSP PFLKSDHPVC HVSGPEVVGS GFQSSVAVRC MLPSAPSTPI 

       610        620        630        640        650        660 
RLPHSAALSQ FHIPGASQFA AARIPPNYQG SVIPSASFDA YRHYMAYAAS RWPMYPASQP 

       670        680        690        700        710        720 
PSHPLSDPHR LLPVTKQAAR SRPNLRVIPT VTPAKPKQEI PVLGSISVKR IPVRVSIPSL 

       730        740        750        760        770        780 
IKYNPKKISD EKNRASQKQK VIEEREKLKT EQEARQKKMF YLTTELERLH KQQGEMLRKK 

       790        800        810        820        830        840 
RREKDGHKDP LLMEVSRLQD SIMKDIAELH KETEEAEKKQ SELDKVAQIL GIDIFDKSLK 

       850        860        870        880        890        900 
SSNDSKESTE KPEKEKSKSP EKELSPSNSS SSNKESKMNE KSCIKSPSST ESLQPTVKQS 

       910        920        930        940        950        960 
DQPVAAYEYY DAGSHWCKDC NTTCGTMFDF FTHMHNKKHT QTLDPYNRPW ASKTQSEAKQ 

       970        980        990       1000       1010       1020 
DTVKRTDKIT VPAKGSEFLI PVTGFYCQLC EEFLGDPISG EQHVKGHQHN ENYKKYVEEN 

      1030       1040       1050       1060       1070       1080 
PLYEERRNLD RQAGLAVVLE TERRRQNELK RKLNEKPKEE KIEKKARIVR EVKEDDKAPG 

      1090       1100       1110       1120       1130       1140 
ELEEQLSEDG SAPEKGEVKG NASLRPQVKE EVKKEPSVAS IAASFGKFSW KKPEKEEEKG 

      1150       1160       1170       1180       1190       1200 
SVVTPGAPKE DTVETSKDRD DGKAEVGKAK PIKIKLSGKT VIAHTSPWTP VVTTSTQTKI 

      1210       1220       1230       1240       1250       1260 
RPNLPIPSTV LRKSGSATVS KPAPLNTFLS IKSSGTSTKP LPVVKESSSD LLLPPDIISK 

      1270       1280       1290       1300       1310       1320 
AFGGEEVVLK GSPEEKVELA EKNEPSQVPE QMLALLPPPP PPPPPPPPPP PPPPPQAVPQ 

      1330       1340       1350       1360       1370       1380 
LSAPSPAQAN VVLTPVKSNS VISQTFSLGF QGPNILNPGL PVAFMASEQP TVIPSDETAP 

      1390       1400       1410       1420       1430       1440 
GVSESDWDQT LISMLVRPPP PLSSVFSEQA KKLEKRNSCL ATANAKDLYD IFYSSGGKGA 

      1450       1460       1470       1480       1490       1500 
HETKLSSSTL ANGESSSLPR TESSDFSSTC TLNSSMSSED LPQCSALVTA TEISNLENPI 

      1510       1520       1530       1540       1550       1560 
SKGMESTGKW SVVDQIDPKS RDSTYSFLQP LTRLYQNKPY EIVSPKTDTL VMWTSGSSQN 

      1570       1580       1590       1600       1610       1620 
DTHKDRPPEG KIRFDLGEPG PPGTDSTSHL SDTHCQTNGP QKLIEINLID NQNKNQEVYQ 

      1630       1640       1650       1660       1670       1680 
SEGCRESEMK RKTELKGKVA TEEEEEEEEE GANSIEDSNS NHGNRNTWEG EIGQPKLSTV 

      1690       1700       1710       1720       1730       1740 
DKKGEQSSKL MTGHENTSKV VIELSPSLPS KRTKIDLFPS LLQNPKSMPE LLLLSPAGSG 

      1750       1760       1770       1780       1790       1800 
LCLKRQEIWE RPEKPGLEDV ELQGTRPELT VTIESKVLEN FDTTHLEVEG FASLRNLGDM 

      1810       1820       1830       1840       1850       1860 
HANFHNSQTE QTRRSPTALS EKMSEEISVS SVMCNPSSSS DIEPVPSFSG FPLESPKTLV 

      1870       1880       1890       1900       1910       1920 
LNFETEGAHS SSNSRNGRIT SNSLETGHPV ENVGHDLGGE RTHQALDLLA GGMLSEDVKE 

      1930       1940       1950       1960       1970       1980 
TSPLQKDLLR MESTTVSPSG LGPSPCLPDL VDFVTRTPGV PKQKPCSPLS EPDAFLKCSS 

      1990       2000       2010       2020       2030       2040 
LEMGSPPPEI LSVSVSEVAV PQVSEDNDSA LNLVKTPPSG SPSRDQVVGG NVSPREMPEQ 

      2050       2060 
EAAVDVIPDH TRSNVYNSQD YLNG 

« Hide

Isoform 2 [UniParc].

Checksum: 8CCE812CE531C1F6
Show »

FASTA981110,832

References

« Hide 'large scale' references
[1]"The transcript for a novel protein with a zinc finger motif is expressed at specific stages of mouse spermatogenesis."
Inoue A., Ishiji A., Kasagi S., Ishizuka M., Hirose S., Baba T., Hagiwara H.
Biochem. Biophys. Res. Commun. 273:398-403(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
Strain: ddY.
Tissue: Testis.
[2]"Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis."
Ishizuka M., Ohshima H., Tamura N., Nakada T., Inoue A., Hirose S., Hagiwara H.
Biochem. Biophys. Res. Commun. 301:1079-1085(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: ddY.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 393-1057 AND 1999-2064 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Embryo.
[4]"A zinc finger protein TZF is a novel corepressor of androgen receptor."
Ishizuka M., Kawate H., Takayanagi R., Ohshima H., Tao R.-H., Hagiwara H.
Biochem. Biophys. Res. Commun. 331:1025-1031(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF159455 mRNA. Translation: AAF61636.1. Frameshift.
AF227194 mRNA. Translation: AAK00650.1. Frameshift.
AK011661 mRNA. Translation: BAC25342.1.
AK157779 mRNA. Translation: BAE34193.1.
PIRJC7316.
RefSeqNP_997554.2. NM_207671.4.
UniGeneMm.439916.

3D structure databases

ProteinModelPortalQ99PP2.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ99PP2.

Proteomic databases

PaxDbQ99PP2.
PRIDEQ99PP2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID57908.
KEGGmmu:57908.

Organism-specific databases

CTD57908.
MGIMGI:1889348. Zfp318.

Phylogenomic databases

eggNOGNOG87509.
HOGENOMHOG000043103.
HOVERGENHBG107139.
InParanoidQ99PP2.

Gene expression databases

CleanExMM_ZFP318.
GenevestigatorQ99PP2.

Family and domain databases

InterProIPR015880. Znf_C2H2-like.
IPR003604. Znf_U1.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZNF318. mouse.
NextBio314073.
PROQ99PP2.
SOURCESearch...

Entry information

Entry nameZN318_MOUSE
AccessionPrimary (citable) accession number: Q99PP2
Secondary accession number(s): Q3TZL5, Q8BMX9, Q9JJ01
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 15, 2008
Last modified: June 11, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot