Q99PP0 (WISP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WNT1-inducible-signaling pathway protein 1
Short name=WISP-1

Alternative name(s):
CCN family member 4
ELM-1

Gene names

Name:	Wisp1
Synonyms:	Ccn4, Elm1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Downstream regulator in the Wnt/Frizzled-signaling pathway By similarity. Associated with cell survival. Adheres to skin and melanoma fibroblasts By similarity. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Biological process	Cell adhesion Wnt signaling pathway
Cellular component	Secreted
Disease	Proto-oncogene
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 367	345	WNT1-inducible-signaling pathway protein 1		PRO_0000014408
Regions
	Domain	45 – 118	74	IGFBP N-terminal
	Domain	121 – 186	66	VWFC
	Domain	215 – 260	46	TSP type-1
	Domain	273 – 347	75	CTCK
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Glycosylation	284	1	N-linked (GlcNAc...) Potential
	Glycosylation	343	1	N-linked (GlcNAc...) Potential
	Disulfide bond	273 ↔ 310		By similarity
	Disulfide bond	290 ↔ 324		By similarity
	Disulfide bond	301 ↔ 340		By similarity
	Disulfide bond	304 ↔ 342		By similarity
	Disulfide bond	309 ↔ 346		By similarity
Experimental info
	Sequence conflict	17	1	V → L in CAB41995. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q99PP0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8A4A34C69D3243D2	FASTA	367	40,614
	10 20 30 40 50 60 MRWLLPWTLA AVAVLMVGNI LATALSPTPT TMTFTPAPLE ETITRPEFCK WPCECPQAPP 70 80 90 100 110 120 RCPLGVSLIT DGCECCKICA QQLGDNCTEA AVCDPHRGLY CDYSGDRPRY AIGVCAQVVG 130 140 150 160 170 180 VGCVLDGVRY TNGESFQPNC RYNCTCIDGT VGCTPLCLSP RPPRLWCRQP RHVRVPGQCC 190 200 210 220 230 240 EQWVCDDDAR RPRQTALLDT RAFAASGAVE QRYENCIAYT SPWSPCSTTC GLGISTRISN 250 260 270 280 290 300 VNARCWPEQE SRLCNLRPCD VDIRPHIKAG KKCLAVYQPE EATNFTLAGC VSTRTYRPKY 310 320 330 340 350 360 CGVCTDNRCC IPYKSKTISV DFQCPEGPGF SRQVLWINAC FCNLSCRNPN DIFADLESYP DFAEIAN

References

[1]	"Gene expression in rat dermal papilla cells: analysis of 2529 ESTs." Sleeman M.A., Murison J.G., Strachan L., Kumble K.D., Glenn M.P., McGrath A., Bickerstaff P., Grierson A., Havukkala I., Tan P., Watson J.D. Genomics 69:214-224(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hair follicle dermal papilla.
[2]	"Partial cDNA-sequence of rat ELM1." Jia J.D., Bauer M., Schuppan D. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-74.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF228049 mRNA. Translation: AAK00729.1. AJ236871 mRNA. Translation: CAB41995.1.
IPI	IPI00201246.
RefSeq	NP_113904.1. NM_031716.1.
UniGene	Rn.63486.
3D structure databases
ProteinModelPortal	Q99PP0.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000009673.
Proteomic databases
PaxDb	Q99PP0.
PRIDE	Q99PP0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	65154.
KEGG	rno:65154.
UCSC	RGD:69431. rat.
Organism-specific databases
CTD	8840.
RGD	69431. Wisp1.
Phylogenomic databases
eggNOG	NOG76761.
HOGENOM	HOG000231462.
HOVERGEN	HBG000635.
InParanoid	Q99PP0.
OrthoDB	EOG4ZKJN7.
Gene expression databases
Genevestigator	Q99PP0.
GermOnline	ENSRNOG00000007078. Rattus norvegicus.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613997.

Entry information

Entry name

WISP1_RAT

Accession

Primary (citable) accession number: Q99PP0
Secondary accession number(s): Q9WUW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program