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Protein

Uridine-cytidine kinase 2

Gene

Uck2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine (By similarity).By similarity

Catalytic activityi

ATP + uridine = ADP + UMP.
ATP + cytidine = ADP + CMP.

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uridine kinase (Uck1), Uridine kinase (Uck2), Uridine kinase (Uck2), Uridine-cytidine kinase 1 (Uck1), Uridine-cytidine kinase 2 (Uck2), Uridine kinase (Uckl1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Uridine-cytidine kinase-like 1 (Uckl1), Uridine kinase (Uck1), Uridine kinase (Uck2), Uridine kinase (Uck2), Uridine-cytidine kinase 1 (Uck1), Uridine-cytidine kinase 2 (Uck2), Uridine kinase (Uckl1)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateBy similarity1
Binding sitei112SubstrateBy similarity1
Binding sitei117SubstrateBy similarity1
Binding sitei166SubstrateBy similarity1
Binding sitei176SubstrateBy similarity1
Binding sitei184SubstrateBy similarity1
Binding sitei213ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73614. Pyrimidine salvage reactions.
UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine-cytidine kinase 2 (EC:2.7.1.48)
Short name:
UCK 2
Alternative name(s):
Cytidine monophosphokinase 2
Uridine monophosphokinase 2
Gene namesi
Name:Uck2
Synonyms:Umpk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1931744. Uck2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001644562 – 261Uridine-cytidine kinase 2Add BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei254PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99PM9.
MaxQBiQ99PM9.
PaxDbiQ99PM9.
PRIDEiQ99PM9.

PTM databases

iPTMnetiQ99PM9.
PhosphoSitePlusiQ99PM9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026558.
CleanExiMM_UCK2.
ExpressionAtlasiQ99PM9. baseline and differential.
GenevisibleiQ99PM9. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Grb2Q606313EBI-644712,EBI-1688

Protein-protein interaction databases

IntActiQ99PM9. 3 interactors.
MINTiMINT-1745079.
STRINGi10090.ENSMUSP00000027839.

Structurei

3D structure databases

ProteinModelPortaliQ99PM9.
SMRiQ99PM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Curated

Phylogenomic databases

eggNOGiENOG410IMEH. Eukaryota.
COG0572. LUCA.
GeneTreeiENSGT00390000015696.
HOGENOMiHOG000262756.
HOVERGENiHBG023339.
InParanoidiQ99PM9.
KOiK00876.
OMAiEHTRTTE.
OrthoDBiEOG091G07OR.
PhylomeDBiQ99PM9.
TreeFamiTF316686.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029925. UCK-2.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF28. PTHR10285:SF28. 1 hit.
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDSEQTLQ NHQQPNGGEP FLIGVSGGTA SGKSSVCAKI VQLLGQNEVD
60 70 80 90 100
YHQKQVVILS QDSFYRVLTS EQKAKALKGQ FNFDHPDAFD NELIFKTLKE
110 120 130 140 150
ITEGKTVQIP VYDFVSHSRK EETVTIYPAD VVLFEGILAF YSQEVRDLFQ
160 170 180 190 200
MKLFVDTDAD TRLSRRVLRD ISERGRDLEQ ILSQYITFVK PAFEEFCLPT
210 220 230 240 250
KKYADVIIPR GADNLVAINL IVQHIQDILN GGLSKRQTNG YLNGYTPSRK
260
RQASESSSRP H
Length:261
Mass (Da):29,404
Last modified:June 1, 2001 - v1
Checksum:i780AA3CFF5CA8153
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF236636 mRNA. Translation: AAK14052.1.
BC023789 mRNA. Translation: AAH23789.1.
CCDSiCCDS15455.1.
RefSeqiNP_109649.1. NM_030724.3.
UniGeneiMm.280895.
Mm.440156.

Genome annotation databases

EnsembliENSMUST00000053686; ENSMUSP00000060202; ENSMUSG00000026558.
GeneIDi80914.
KEGGimmu:80914.
UCSCiuc007dku.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF236636 mRNA. Translation: AAK14052.1.
BC023789 mRNA. Translation: AAH23789.1.
CCDSiCCDS15455.1.
RefSeqiNP_109649.1. NM_030724.3.
UniGeneiMm.280895.
Mm.440156.

3D structure databases

ProteinModelPortaliQ99PM9.
SMRiQ99PM9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99PM9. 3 interactors.
MINTiMINT-1745079.
STRINGi10090.ENSMUSP00000027839.

PTM databases

iPTMnetiQ99PM9.
PhosphoSitePlusiQ99PM9.

Proteomic databases

EPDiQ99PM9.
MaxQBiQ99PM9.
PaxDbiQ99PM9.
PRIDEiQ99PM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053686; ENSMUSP00000060202; ENSMUSG00000026558.
GeneIDi80914.
KEGGimmu:80914.
UCSCiuc007dku.2. mouse.

Organism-specific databases

CTDi7371.
MGIiMGI:1931744. Uck2.

Phylogenomic databases

eggNOGiENOG410IMEH. Eukaryota.
COG0572. LUCA.
GeneTreeiENSGT00390000015696.
HOGENOMiHOG000262756.
HOVERGENiHBG023339.
InParanoidiQ99PM9.
KOiK00876.
OMAiEHTRTTE.
OrthoDBiEOG091G07OR.
PhylomeDBiQ99PM9.
TreeFamiTF316686.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.
ReactomeiR-MMU-73614. Pyrimidine salvage reactions.

Miscellaneous databases

ChiTaRSiUck2. mouse.
PROiQ99PM9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026558.
CleanExiMM_UCK2.
ExpressionAtlasiQ99PM9. baseline and differential.
GenevisibleiQ99PM9. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029925. UCK-2.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF28. PTHR10285:SF28. 1 hit.
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUCK2_MOUSE
AccessioniPrimary (citable) accession number: Q99PM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.