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Q99PM3

- TF2AA_MOUSE

UniProt

Q99PM3 - TF2AA_MOUSE

Protein

Transcription initiation factor IIA subunit 1

Gene

Gtf2a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei276 – 2772Cleavage; by TASP1

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. RNA polymerase II repressing transcription factor binding Source: MGI

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription initiation from RNA polymerase II promoter Source: InterPro

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor IIA subunit 1
    Alternative name(s):
    General transcription factor IIA subunit 1
    Cleaved into the following 2 chains:
    Alternative name(s):
    TFIIA p35 subunit
    Alternative name(s):
    TFIIA p19 subunit
    Gene namesi
    Name:Gtf2a1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1933277. Gtf2a1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI
    3. transcription factor TFIIA complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 378377Transcription initiation factor IIA subunit 1PRO_0000042595Add
    BLAST
    Chaini2 – 276275Transcription initiation factor IIA alpha chainPRO_0000042596Add
    BLAST
    Chaini277 – 378102Transcription initiation factor IIA beta chainPRO_0000042597Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei282 – 2821Phosphoserine; by TAF1By similarity
    Modified residuei283 – 2831Phosphoserine; by TAF1By similarity
    Modified residuei318 – 3181Phosphoserine; by TAF1By similarity
    Modified residuei323 – 3231Phosphoserine; by TAF1By similarity

    Post-translational modificationi

    The alpha and beta subunits are postranslationally produced from the precursor form by TASP1. The cleavage promotes proteasomal degradation By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ99PM3.
    PaxDbiQ99PM3.
    PRIDEiQ99PM3.

    PTM databases

    PhosphoSiteiQ99PM3.

    Expressioni

    Tissue specificityi

    Expressed in pachytene spermatocytes and spermatids.1 Publication

    Inductioni

    Up-regulated during germ cell differentiation in testis.1 Publication

    Gene expression databases

    ArrayExpressiQ99PM3.
    BgeeiQ99PM3.
    CleanExiMM_GTF2A1.
    GenevestigatoriQ99PM3.

    Interactioni

    Subunit structurei

    TFIIA is a heterodimer of a unprocessed large subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha, a beta and a gamma subunit. TFIIA forms a complex with TBP By similarity.By similarity

    Protein-protein interaction databases

    BioGridi219952. 13 interactions.
    MINTiMINT-1348159.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99PM3.
    SMRiQ99PM3. Positions 9-51, 332-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi71 – 8010Poly-Gln
    Compositional biasi81 – 888Poly-His

    Sequence similaritiesi

    Belongs to the TFIIA subunit 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG5149.
    GeneTreeiENSGT00530000063152.
    HOGENOMiHOG000015236.
    HOVERGENiHBG052771.
    InParanoidiQ99PM3.
    KOiK03122.
    OMAiHMSATGM.
    OrthoDBiEOG7KWSJM.
    PhylomeDBiQ99PM3.
    TreeFamiTF350445.

    Family and domain databases

    Gene3Di1.10.287.100. 1 hit.
    2.30.18.10. 1 hit.
    InterProiIPR009083. TFIIA_a-hlx.
    IPR004855. TFIIA_asu/bsu.
    IPR013028. TFIIA_asu_N.
    IPR009088. TFIIA_b-brl.
    [Graphical view]
    PANTHERiPTHR12694. PTHR12694. 1 hit.
    PfamiPF03153. TFIIA. 1 hit.
    [Graphical view]
    SUPFAMiSSF47396. SSF47396. 1 hit.
    SSF50784. SSF50784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99PM3-1 [UniParc]FASTAAdd to Basket

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    MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL    50
    MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHQHQQ AQPQQTVPQQ 100
    AQTQQVLIPA SQQATAPQVI VPDSKLLQHM NASSITSAAA TAATLALPAG 150
    VTPVQQLLTN SGQLLQVVRA ANGAQYILQP QQSVVLQQQV IPQMQPGGVQ 200
    APVIQQVLAP LPGGISPQTG VIIQPQQILF TGNKTQVIPT TVAAPAPAQA 250
    PMPAAGQQQP QAQPAQQQAP LVLQVDGTGD TSSEEDEDEE EDYDDDEEED 300
    KEKDGAEDGQ VEEEPLNSED DVSDEEGQEL FDTENVVVCQ YDKIHRSKNK 350
    WKFHLKDGIM NLNGRDYIFS KAIGDAEW 378
    Length:378
    Mass (Da):41,614
    Last modified:June 7, 2005 - v2
    Checksum:iFE28AA688662457E
    GO

    Sequence cautioni

    The sequence BAC33430.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti121 – 1211V → G in AAG50431. (PubMed:11159353)Curated
    Sequence conflicti324 – 3241D → G in AAG50431. (PubMed:11159353)Curated
    Sequence conflicti333 – 3331T → A in AAG50431. (PubMed:11159353)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF250834 mRNA. Translation: AAG50431.1.
    AK048711 mRNA. Translation: BAC33430.1. Different initiation.
    CCDSiCCDS26089.1.
    RefSeqiNP_113568.2. NM_031391.2.
    NP_780544.1. NM_175335.3.
    UniGeneiMm.275728.
    Mm.432242.

    Genome annotation databases

    EnsembliENSMUST00000021345; ENSMUSP00000021345; ENSMUSG00000020962.
    GeneIDi83602.
    KEGGimmu:83602.
    UCSCiuc007okr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF250834 mRNA. Translation: AAG50431.1 .
    AK048711 mRNA. Translation: BAC33430.1 . Different initiation.
    CCDSi CCDS26089.1.
    RefSeqi NP_113568.2. NM_031391.2.
    NP_780544.1. NM_175335.3.
    UniGenei Mm.275728.
    Mm.432242.

    3D structure databases

    ProteinModelPortali Q99PM3.
    SMRi Q99PM3. Positions 9-51, 332-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 219952. 13 interactions.
    MINTi MINT-1348159.

    PTM databases

    PhosphoSitei Q99PM3.

    Proteomic databases

    MaxQBi Q99PM3.
    PaxDbi Q99PM3.
    PRIDEi Q99PM3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021345 ; ENSMUSP00000021345 ; ENSMUSG00000020962 .
    GeneIDi 83602.
    KEGGi mmu:83602.
    UCSCi uc007okr.2. mouse.

    Organism-specific databases

    CTDi 2957.
    MGIi MGI:1933277. Gtf2a1.

    Phylogenomic databases

    eggNOGi COG5149.
    GeneTreei ENSGT00530000063152.
    HOGENOMi HOG000015236.
    HOVERGENi HBG052771.
    InParanoidi Q99PM3.
    KOi K03122.
    OMAi HMSATGM.
    OrthoDBi EOG7KWSJM.
    PhylomeDBi Q99PM3.
    TreeFami TF350445.

    Miscellaneous databases

    NextBioi 350672.
    PROi Q99PM3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99PM3.
    Bgeei Q99PM3.
    CleanExi MM_GTF2A1.
    Genevestigatori Q99PM3.

    Family and domain databases

    Gene3Di 1.10.287.100. 1 hit.
    2.30.18.10. 1 hit.
    InterProi IPR009083. TFIIA_a-hlx.
    IPR004855. TFIIA_asu/bsu.
    IPR013028. TFIIA_asu_N.
    IPR009088. TFIIA_b-brl.
    [Graphical view ]
    PANTHERi PTHR12694. PTHR12694. 1 hit.
    Pfami PF03153. TFIIA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47396. SSF47396. 1 hit.
    SSF50784. SSF50784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse."
      Han S., Zhou L., Upadhyaya A.B., Lee S.H., Parker K.L., DeJong J.
      Biol. Reprod. 64:507-517(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-378.
      Strain: C57BL/6J.
      Tissue: Cerebellum.

    Entry informationi

    Entry nameiTF2AA_MOUSE
    AccessioniPrimary (citable) accession number: Q99PM3
    Secondary accession number(s): Q8C812
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3