SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q99PM3

- TF2AA_MOUSE

UniProt

Q99PM3 - TF2AA_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcription initiation factor IIA subunit 1

Gene
Gtf2a1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei276 – 2772Cleavage; by TASP1

GO - Molecular functioni

  1. protein binding Source: MGI
  2. RNA polymerase II repressing transcription factor binding Source: MGI

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription initiation from RNA polymerase II promoter Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIA subunit 1
Alternative name(s):
General transcription factor IIA subunit 1
Cleaved into the following 2 chains:
Alternative name(s):
TFIIA p35 subunit
Alternative name(s):
TFIIA p19 subunit
Gene namesi
Name:Gtf2a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1933277. Gtf2a1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
  3. transcription factor TFIIA complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 378377Transcription initiation factor IIA subunit 1PRO_0000042595Add
BLAST
Chaini2 – 276275Transcription initiation factor IIA alpha chainPRO_0000042596Add
BLAST
Chaini277 – 378102Transcription initiation factor IIA beta chainPRO_0000042597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei282 – 2821Phosphoserine; by TAF1 By similarity
Modified residuei283 – 2831Phosphoserine; by TAF1 By similarity
Modified residuei318 – 3181Phosphoserine; by TAF1 By similarity
Modified residuei323 – 3231Phosphoserine; by TAF1 By similarity

Post-translational modificationi

The alpha and beta subunits are postranslationally produced from the precursor form by TASP1. The cleavage promotes proteasomal degradation By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99PM3.
PaxDbiQ99PM3.
PRIDEiQ99PM3.

PTM databases

PhosphoSiteiQ99PM3.

Expressioni

Tissue specificityi

Expressed in pachytene spermatocytes and spermatids.1 Publication

Inductioni

Up-regulated during germ cell differentiation in testis.1 Publication

Gene expression databases

ArrayExpressiQ99PM3.
BgeeiQ99PM3.
CleanExiMM_GTF2A1.
GenevestigatoriQ99PM3.

Interactioni

Subunit structurei

TFIIA is a heterodimer of a unprocessed large subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha, a beta and a gamma subunit. TFIIA forms a complex with TBP By similarity.

Protein-protein interaction databases

BioGridi219952. 13 interactions.
MINTiMINT-1348159.

Structurei

3D structure databases

ProteinModelPortaliQ99PM3.
SMRiQ99PM3. Positions 9-51, 332-378.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi71 – 8010Poly-Gln
Compositional biasi81 – 888Poly-His

Sequence similaritiesi

Belongs to the TFIIA subunit 1 family.

Phylogenomic databases

eggNOGiCOG5149.
GeneTreeiENSGT00530000063152.
HOGENOMiHOG000015236.
HOVERGENiHBG052771.
InParanoidiQ99PM3.
KOiK03122.
OMAiHMSATGM.
OrthoDBiEOG7KWSJM.
PhylomeDBiQ99PM3.
TreeFamiTF350445.

Family and domain databases

Gene3Di1.10.287.100. 1 hit.
2.30.18.10. 1 hit.
InterProiIPR009083. TFIIA_a-hlx.
IPR004855. TFIIA_asu/bsu.
IPR013028. TFIIA_asu_N.
IPR009088. TFIIA_b-brl.
[Graphical view]
PANTHERiPTHR12694. PTHR12694. 1 hit.
PfamiPF03153. TFIIA. 1 hit.
[Graphical view]
SUPFAMiSSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PM3-1 [UniParc]FASTAAdd to Basket

« Hide

MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL    50
MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHQHQQ AQPQQTVPQQ 100
AQTQQVLIPA SQQATAPQVI VPDSKLLQHM NASSITSAAA TAATLALPAG 150
VTPVQQLLTN SGQLLQVVRA ANGAQYILQP QQSVVLQQQV IPQMQPGGVQ 200
APVIQQVLAP LPGGISPQTG VIIQPQQILF TGNKTQVIPT TVAAPAPAQA 250
PMPAAGQQQP QAQPAQQQAP LVLQVDGTGD TSSEEDEDEE EDYDDDEEED 300
KEKDGAEDGQ VEEEPLNSED DVSDEEGQEL FDTENVVVCQ YDKIHRSKNK 350
WKFHLKDGIM NLNGRDYIFS KAIGDAEW 378
Length:378
Mass (Da):41,614
Last modified:June 7, 2005 - v2
Checksum:iFE28AA688662457E
GO

Sequence cautioni

The sequence BAC33430.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211V → G in AAG50431. 1 Publication
Sequence conflicti324 – 3241D → G in AAG50431. 1 Publication
Sequence conflicti333 – 3331T → A in AAG50431. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF250834 mRNA. Translation: AAG50431.1.
AK048711 mRNA. Translation: BAC33430.1. Different initiation.
CCDSiCCDS26089.1.
RefSeqiNP_113568.2. NM_031391.2.
NP_780544.1. NM_175335.3.
UniGeneiMm.275728.
Mm.432242.

Genome annotation databases

EnsembliENSMUST00000021345; ENSMUSP00000021345; ENSMUSG00000020962.
GeneIDi83602.
KEGGimmu:83602.
UCSCiuc007okr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF250834 mRNA. Translation: AAG50431.1 .
AK048711 mRNA. Translation: BAC33430.1 . Different initiation.
CCDSi CCDS26089.1.
RefSeqi NP_113568.2. NM_031391.2.
NP_780544.1. NM_175335.3.
UniGenei Mm.275728.
Mm.432242.

3D structure databases

ProteinModelPortali Q99PM3.
SMRi Q99PM3. Positions 9-51, 332-378.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 219952. 13 interactions.
MINTi MINT-1348159.

PTM databases

PhosphoSitei Q99PM3.

Proteomic databases

MaxQBi Q99PM3.
PaxDbi Q99PM3.
PRIDEi Q99PM3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021345 ; ENSMUSP00000021345 ; ENSMUSG00000020962 .
GeneIDi 83602.
KEGGi mmu:83602.
UCSCi uc007okr.2. mouse.

Organism-specific databases

CTDi 2957.
MGIi MGI:1933277. Gtf2a1.

Phylogenomic databases

eggNOGi COG5149.
GeneTreei ENSGT00530000063152.
HOGENOMi HOG000015236.
HOVERGENi HBG052771.
InParanoidi Q99PM3.
KOi K03122.
OMAi HMSATGM.
OrthoDBi EOG7KWSJM.
PhylomeDBi Q99PM3.
TreeFami TF350445.

Miscellaneous databases

NextBioi 350672.
PROi Q99PM3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99PM3.
Bgeei Q99PM3.
CleanExi MM_GTF2A1.
Genevestigatori Q99PM3.

Family and domain databases

Gene3Di 1.10.287.100. 1 hit.
2.30.18.10. 1 hit.
InterProi IPR009083. TFIIA_a-hlx.
IPR004855. TFIIA_asu/bsu.
IPR013028. TFIIA_asu_N.
IPR009088. TFIIA_b-brl.
[Graphical view ]
PANTHERi PTHR12694. PTHR12694. 1 hit.
Pfami PF03153. TFIIA. 1 hit.
[Graphical view ]
SUPFAMi SSF47396. SSF47396. 1 hit.
SSF50784. SSF50784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse."
    Han S., Zhou L., Upadhyaya A.B., Lee S.H., Parker K.L., DeJong J.
    Biol. Reprod. 64:507-517(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-378.
    Strain: C57BL/6J.
    Tissue: Cerebellum.

Entry informationi

Entry nameiTF2AA_MOUSE
AccessioniPrimary (citable) accession number: Q99PM3
Secondary accession number(s): Q8C812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi