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Protein

Probable E3 ubiquitin-protein ligase TRIM8

Gene

Trim8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 56RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri92 – 132B box-type 1Add BLAST41
Zinc fingeri140 – 182B box-type 2Add BLAST43

GO - Molecular functioni

GO - Biological processi

  • innate immune response Source: UniProtKB
  • negative regulation of viral entry into host cell Source: UniProtKB
  • negative regulation of viral release from host cell Source: UniProtKB
  • negative regulation of viral transcription Source: UniProtKB
  • positive regulation of autophagy Source: UniProtKB
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • positive regulation of NF-kappaB transcription factor activity Source: MGI
  • positive regulation of protein localization to nucleus Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • stem cell population maintenance Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase TRIM8 (EC:2.3.2.27)
Alternative name(s):
Glioblastoma-expressed RING finger protein
RING finger protein 27
RING-type E3 ubiquitin transferase TRIM8Curated
Tripartite motif-containing protein 8
Gene namesi
Name:Trim8
Synonyms:Gerp, Rnf27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1933302. Trim8.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • nucleus Source: MGI
  • PML body Source: MGI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562071 – 551Probable E3 ubiquitin-protein ligase TRIM8Add BLAST551

Proteomic databases

MaxQBiQ99PJ2.
PaxDbiQ99PJ2.
PRIDEiQ99PJ2.
TopDownProteomicsiQ99PJ2.

PTM databases

PhosphoSitePlusiQ99PJ2.

Expressioni

Tissue specificityi

High expression in heart, liver, and thymus. Expressed in embryonic CNS, kidney, lens and gut.2 Publications

Developmental stagei

At 10.5 and 12.5 dpc, expressed in the central nervous system. At 14.5 dpc, expressed in the eye (lens and inner neural layer of the retina), in the primitive glomeruli of the developing kidney, in the villi of the gut and in the dorsal root ganglia.

Gene expression databases

BgeeiENSMUSG00000025034.
CleanExiMM_TRIM8.
GenevisibleiQ99PJ2. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with SOCS1 (via) SH2 domain and SOCS box. Interacts with HSP90AB1; prevents nucleus translocation of phosphorylated STAT3 and HSP90AB1 (PubMed:21689689).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220231. 1 interactor.
IntActiQ99PJ2. 1 interactor.
MINTiMINT-1634563.
STRINGi10090.ENSMUSP00000026008.

Structurei

3D structure databases

ProteinModelPortaliQ99PJ2.
SMRiQ99PJ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili181 – 249Sequence analysisAdd BLAST69
Coiled coili274 – 295Sequence analysisAdd BLAST22

Domaini

The coiled coil domain is required for homodimerization.By similarity
The region immediately C-terminal to the RING motif is sufficient to mediate the interaction with SOCS1.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 56RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri92 – 132B box-type 1Add BLAST41
Zinc fingeri140 – 182B box-type 2Add BLAST43

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KDQ2. Eukaryota.
ENOG410ZTPN. LUCA.
GeneTreeiENSGT00850000132354.
HOGENOMiHOG000082538.
HOVERGENiHBG056254.
InParanoidiQ99PJ2.
KOiK12001.
OMAiHPPYPRS.
OrthoDBiEOG091G059M.
TreeFamiTF333491.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99PJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENWKNCFE EELICPICLH VFVEPVQLPC KHNFCRGCIG EAWAKDSGLV
60 70 80 90 100
RCPECNQAYN QKPGLEKNLK LTNIVEKFNA LHVEKPPTAL HCVFCRRGPP
110 120 130 140 150
LPAQKVCLRC EAPCCQSHVQ THLQQPSTAR GHLLVEADDV RAWSCPQHNA
160 170 180 190 200
YRLYHCEAEQ VAVCQYCCYY SGAHQGHSVC DVEIRRNEIR KMLMKQQERL
210 220 230 240 250
EEREQDIEDQ LYKLESDKRL VEEKVSQLKE EVRLQYEKLH QLLDEDLRQT
260 270 280 290 300
VEVLDKAQAK FCSENAAQAL HLGERMQEAK KLLGSLQRLF DKTEDVGFMK
310 320 330 340 350
NTKSVKILMD RTQTCTGSSL SPPKIGHLNS KLFLNEVAKK EKQLRKMLEG
360 370 380 390 400
PFSTPVPFLQ SVPLYPCGVN SSGAEKRKHS TAFPEASFLE TSSGPVGGQY
410 420 430 440 450
GAAGTASSEG QSGQPLGPCS STQHLVALPG GTQPVHSSPV FPPSQYPNGS
460 470 480 490 500
TTQQPMLPQY GGRKILVCSV DNCYCSSVAN HGGHQPYPRS GHFPWTVPSQ
510 520 530 540 550
EYSHPLPPTP SVPQSLPGLA VRDWLDASQQ PGHQDFYRVY GQPSTKHYVT

S
Length:551
Mass (Da):61,602
Last modified:July 27, 2011 - v3
Checksum:iA5388F450D6566A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti218K → M in AAG53088 (PubMed:11118312).Curated1
Sequence conflicti218K → M in AAG53489 (PubMed:11331580).Curated1
Sequence conflicti259A → G in AAG53088 (PubMed:11118312).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF281047 mRNA. Translation: AAG53088.1.
AK052763 mRNA. Translation: BAC35138.1.
AK079847 mRNA. Translation: BAC37764.1.
AK155303 mRNA. Translation: BAE33177.1.
BC037065 mRNA. Translation: AAH37065.1.
BC152343 mRNA. Translation: AAI52344.1.
BC152344 mRNA. Translation: AAI52345.1.
AF220035 mRNA. Translation: AAG53489.1.
CCDSiCCDS38009.1.
RefSeqiNP_444330.2. NM_053100.2.
UniGeneiMm.392177.
Mm.481672.

Genome annotation databases

EnsembliENSMUST00000026008; ENSMUSP00000026008; ENSMUSG00000025034.
GeneIDi93679.
KEGGimmu:93679.
UCSCiuc008hts.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiTRIM8_MOUSE
AccessioniPrimary (citable) accession number: Q99PJ2
Secondary accession number(s): Q3U2G3
, Q8C508, Q8C700, Q8CGI2, Q99PQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 27, 2011
Last modified: August 30, 2017
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families