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Q99PJ1 (PCD15_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protocadherin-15
Gene names
Name:Pcdh15
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1943 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.

Subunit structure

Interacts with MYO7A. Interacts with CDH23. Ref.5 Ref.6

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity.

Tissue specificity

Expressed in brain and sensory epithelium of the developing inner ear. Also found in the spleen, developing eye, dorsal root ganglion, dorsal aspect of neural tube, floor plate and ependymal cells adjacent to the neural canal. Ref.4 Ref.5 Ref.6

Developmental stage

Highest level of expression is detected at embryonic day 16.

Involvement in disease

Defects in Pcdh15 are the cause of the Ames waltzer (av) phenotype. It is characterized by deafness and a balance disorder, associated with the degeneration of inner ear neuroepithelia.

Sequence similarities

Contains 11 cadherin domains.

Ontologies

Keywords
   Biological processCell adhesion
Hearing
   Cellular componentCell membrane
Membrane
   DiseaseDeafness
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
   PTMGlycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament organization

Inferred from mutant phenotype PubMed 11124469. Source: MGI

adult walking behavior

Inferred from mutant phenotype PubMed 11124469. Source: MGI

auditory receptor cell stereocilium organization

Inferred from mutant phenotype PubMed 10978835PubMed 11124469PubMed 16887306. Source: MGI

detection of mechanical stimulus involved in equilibrioception

Inferred from mutant phenotype Ref.5. Source: MGI

detection of mechanical stimulus involved in sensory perception of sound

Inferred from mutant phenotype Ref.5. Source: MGI

homophilic cell adhesion

Inferred from electronic annotation. Source: InterPro

morphogenesis of an epithelium

Inferred from mutant phenotype Ref.1. Source: MGI

multicellular organism growth

Inferred from mutant phenotype PubMed 10978835. Source: MGI

nonmotile primary cilium assembly

Inferred from mutant phenotype Ref.5. Source: MGI

righting reflex

Inferred from mutant phenotype PubMed 10978835PubMed 15811708. Source: MGI

startle response

Inferred from mutant phenotype PubMed 15811708. Source: MGI

visual perception

Inferred from mutant phenotype PubMed 16799054. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay Ref.5. Source: MGI

integral to membrane

Inferred from sequence or structural similarity Ref.1. Source: MGI

photoreceptor outer segment

Inferred from direct assay PubMed 14570705. Source: MGI

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

stereocilium

Inferred from direct assay PubMed 14570705. Source: HGNC

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 19431917Protocadherin-15
PRO_0000003999

Regions

Topological domain27 – 13811355Extracellular Potential
Transmembrane1382 – 140221Helical; Potential
Topological domain1403 – 1943541Cytoplasmic Potential
Domain45 – 152108Cadherin 1
Domain153 – 270118Cadherin 2
Domain283 – 400118Cadherin 3
Domain401 – 514114Cadherin 4
Domain515 – 621107Cadherin 5
Domain622 – 722101Cadherin 6
Domain724 – 824101Cadherin 7
Domain825 – 931107Cadherin 8
Domain932 – 1040109Cadherin 9
Domain1042 – 1149108Cadherin 10
Domain1150 – 1264115Cadherin 11
Compositional bias1437 – 144812Poly-Pro
Compositional bias1772 – 17787Poly-Pro
Compositional bias1804 – 18129Poly-Pro

Amino acid modifications

Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation1021N-linked (GlcNAc...) Potential
Glycosylation2061N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation5641N-linked (GlcNAc...) Potential
Glycosylation6671N-linked (GlcNAc...) Potential
Glycosylation7291N-linked (GlcNAc...) Potential
Glycosylation7731N-linked (GlcNAc...) Potential
Glycosylation8261N-linked (GlcNAc...) Potential
Glycosylation8561N-linked (GlcNAc...) Potential
Glycosylation10691N-linked (GlcNAc...) Potential
Glycosylation10891N-linked (GlcNAc...) Potential
Glycosylation11801N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict34 – 363FEN → YED in AAG53891. Ref.1
Sequence conflict6081I → T in AAG53891. Ref.1
Sequence conflict9011V → A in AAG53891. Ref.1
Sequence conflict1406 – 14094HFQR → QFKV in AAG53891. Ref.1
Sequence conflict17481S → F in AAG53891. Ref.1
Sequence conflict18481S → F in AAG53891. Ref.1

Secondary structure

1 ......................................................... 1943
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q99PJ1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 7E4AEDD326F6D9F4

FASTA1,943214,787
        10         20         30         40         50         60 
MFLQFAVWKC LPHGILIASL LVVSWGQYDD DWQFENCKLA RGGPPATIVA IDEESRNGTI 

        70         80         90        100        110        120 
LVDNMLIKGT AGGPDPTIEL SLKDNVDYWV LLDPVKQMLF LNSTGRVLDR DPPMNIHSIV 

       130        140        150        160        170        180 
VQVQCVNKKV GTVIYHEVRI VVRDRNDNSP TFKHESYYAT VNELTPVGTT IFTGFSGDNG 

       190        200        210        220        230        240 
ATDIDDGPNG QIEYVIQYNP EDPTSNDTFE IPLMLTGNVV LRKRLNYEDK TRYYVIIQAN 

       250        260        270        280        290        300 
DRAQNLNERR TTTTTLTVDV LDGDDLGPMF LPCVLVPNTR DCRPLTYQAA IPELRTPEEL 

       310        320        330        340        350        360 
NPILVTPPIQ AIDQDRNIQP PSDRPGILYS ILVGTPEDYP RFFHMHPRTA ELTLLEPVNR 

       370        380        390        400        410        420 
DFHQKFDLVI KAEQDNGHPL PAFASLHIEI LDENNQSPYF TMPSYQGYIL ESAPVGATIS 

       430        440        450        460        470        480 
ESLNLTTPLR IVALDKDIED TKDPELHLFL NDYTSVFTVT PTGITRYLTL LQPVDREEQQ 

       490        500        510        520        530        540 
TYTFLITAFD GVQESEPVVV NIRVMDANDN TPTFPEISYD VYVYTDMSPG DSVIQLTAVD 

       550        560        570        580        590        600 
ADEGSNGEIS YEILVGGKGD FVINKTTGLV SIAPGVELIV GQTYALTVQA SDNAPPAERR 

       610        620        630        640        650        660 
HSICTVYIEV LPPNNQSPPR FPQLMYSLEV SEAMRIGAIL LNLQATDREG DPITYAIENG 

       670        680        690        700        710        720 
DPQRVFNLSE TTGILSLGKA LDRESTDRYI LIVTASDGRP DGTSTATVNI VVTDVNDNAP 

       730        740        750        760        770        780 
VFDPYLPRNL SVVEEEANAF VGQVRATDPD AGINGQVHYS LGNFNNLFRI TSNGSIYTAV 

       790        800        810        820        830        840 
KLNREARDHY ELVVVATDGA VHPRHSTLTL YIKVLDIDDN SPVFTNSTYT VVVEENLPAG 

       850        860        870        880        890        900 
TSFLQIEAKD VDLGANVSYR IRSPEVKHLF ALHPFTGELS LLRSLDYEAF PDQEASITFL 

       910        920        930        940        950        960 
VEAFDIYGTM PPGIATVTVI VKDMNDYPPV FSKRIYKGMV APDAVKGTPI TTVYAEDADP 

       970        980        990       1000       1010       1020 
PGMPASRVRY RVDDVQFPYP ASIFDVEEDS GRVVTRVNLN EEPTTIFKLV VVAFDDGEPV 

      1030       1040       1050       1060       1070       1080 
MSSSATVRIL VLHPGEIPRF TQEEYRPPPV SELAARGTVV GVISAAAINQ SIVYSIVAGN 

      1090       1100       1110       1120       1130       1140 
EEDKFGINNV TGVIYVNSPL DYETRTSYVL RVQADSLEVV LANLRVPSKS NTAKVYIEIQ 

      1150       1160       1170       1180       1190       1200 
DENDHPPVFQ KKFYIGGVSE DARMFASVLR VKATDRDTGN YSAMAYRLII PPIKEGKEGF 

      1210       1220       1230       1240       1250       1260 
VVETYTGLIK TAMLFHNMRR SYFKFQVIAT DDYGKGLSGK ADVLVSVVNQ LDMQVIVSNV 

      1270       1280       1290       1300       1310       1320 
PPTLVEKKIE DLTEILDRYV QEQIPGAKVV VESIGARRHG DAYSLEDYSK CDLTVYAIDP 

      1330       1340       1350       1360       1370       1380 
QTNRAIDRNE LFKFLDGKLL DINKDFQPYY GEGGRILEIR TPEAVTSIKK RGESLGYTEG 

      1390       1400       1410       1420       1430       1440 
ALLALAFIII LCCIPAILVV LVSYRHFQRR QAECTKTARI QSAMPAAKPA APVPAAPAPP 

      1450       1460       1470       1480       1490       1500 
PPPPPPPPGA HLYEELGESA MHNLFLLYHF EQSRGNNSVP EDRSSHRDGM AFSSSTTESH 

      1510       1520       1530       1540       1550       1560 
EPAHVEGPLK ESQPNPARTF SFVPDEDNLS THNPLYMESI GQRSTNSDLQ PRTDFEELLA 

      1570       1580       1590       1600       1610       1620 
PRTQVKSQSL RGPREKIQRV WNQSVSFPRR LMWKAPNRPE TIDLVEWQIT NQRAECESAR 

      1630       1640       1650       1660       1670       1680 
CHPSQRGSSN VLLATEDAHE SEKEGGHRDT LIVQQTEQLK SLSSGSSFSS SWSHFSFSTL 

      1690       1700       1710       1720       1730       1740 
PTISRAVELG SEPNVVTSPA DCTLELSPPL RPRILNSLSS KRETPTCASD TEPKRNSFEI 

      1750       1760       1770       1780       1790       1800 
APHPPSISAP LPHPPLPRPP IAFTTFPLPL SPPNPPPPQL VTFSLPISTP PTSSLPLPPP 

      1810       1820       1830       1840       1850       1860 
LSLPPPPRPP APRLFPQPPS TSIPSTDSIS APAAKCTASA THARETTSTT QPPASNPQWG 

      1870       1880       1890       1900       1910       1920 
AEPHRHPKGI LRHVKNLAEL EKSVSNMYSH IEKNCPPADP SKLHTFCPAE KTGMKITHDQ 

      1930       1940 
SQETLVRVVE GIDVQPHSQS TSL

« Hide

References

« Hide 'large scale' references
[1]"The mouse Ames waltzer hearing-loss mutant is caused by mutation of Pcdh15, a novel protocadherin gene."
Alagramam K.N., Murcia C.L., Kwon H.Y., Pawlowski K.S., Wright C.G., Woychik R.P.
Nat. Genet. 27:99-102(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1876-1882, MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[4]"Expression of Pcdh15 in the inner ear, nervous system and various epithelia of the developing embryo."
Murcia C.L., Woychik R.P.
Mech. Dev. 105:163-166(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Physical and functional interaction between protocadherin 15 and myosin VIIa in mechanosensory hair cells."
Senften M., Schwander M., Kazmierczak P., Lillo C., Shin J.B., Hasson T., Geleoc G.S., Gillespie P.G., Williams D., Holt J.R., Muller U.
J. Neurosci. 26:2060-2071(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MYO7A, TISSUE SPECIFICITY.
[6]"Cadherin 23 and protocadherin 15 interact to form tip-link filaments in sensory hair cells."
Kazmierczak P., Sakaguchi H., Tokita J., Wilson-Kubalek E.M., Milligan R.A., Muller U., Kachar B.
Nature 449:87-91(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDH23, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF281899 mRNA. Translation: AAG53891.1.
AC108392 Genomic DNA. No translation available.
AC119894 Genomic DNA. No translation available.
AC121142 Genomic DNA. No translation available.
AC121602 Genomic DNA. No translation available.
AC121832 Genomic DNA. No translation available.
AC123032 Genomic DNA. No translation available.
AC123809 Genomic DNA. No translation available.
AC144802 Genomic DNA. No translation available.
AC147721 Genomic DNA. No translation available.
AC153858 Genomic DNA. No translation available.
AC158800 Genomic DNA. No translation available.
AC159477 Genomic DNA. No translation available.
AC186813 Genomic DNA. No translation available.
AC188091 Genomic DNA. No translation available.
CAAA01110489 Genomic DNA. No translation available.
IPIIPI00121093.
RefSeqNP_075604.2. NM_023115.3.
UniGeneMm.338933.
Mm.490709.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4APXX-ray1.65B27-259[»]
4AQ8X-ray2.63C/D27-259[»]
4AQAX-ray1.96B27-259[»]
4AQEX-ray2.27B27-259[»]
4AXWX-ray2.23B27-259[»]
SMRQ99PJ1. Positions 27-260.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-42151N.
MINTMINT-1895732.
STRING10090.ENSMUSP00000101066.

PTM databases

PhosphoSiteQ99PJ1.

Proteomic databases

PRIDEQ99PJ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID11994.
KEGGmmu:11994.
UCSCuc007fpk.2. mouse.

Organism-specific databases

CTD65217.
MGIMGI:1891428. Pcdh15.

Phylogenomic databases

eggNOGNOG253862.
HOGENOMHOG000230919.
HOVERGENHBG053521.
InParanoidQ99PJ1.
KOK16500.

Gene expression databases

CleanExMM_PCDH15.
GenevestigatorQ99PJ1.
GermOnlineENSMUSG00000052613. Mus musculus.

Family and domain databases

Gene3D2.60.40.60. 10 hits.
InterProIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
[Graphical view]
PfamPF00028. Cadherin. 8 hits.
[Graphical view]
PRINTSPR00205. CADHERIN.
SMARTSM00112. CA. 11 hits.
[Graphical view]
SUPFAMSSF49313. Cadherin. 10 hits.
PROSITEPS00232. CADHERIN_1. 4 hits.
PS50268. CADHERIN_2. 11 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio280177.
SOURCESearch...

Entry information

Entry namePCD15_MOUSE
AccessionPrimary (citable) accession number: Q99PJ1
Secondary accession number(s): E9Q7R1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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