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Protein

Leucine-rich repeat-containing protein 4

Gene

Lrrc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Synaptic adhesion protein. Regulates the formation of exitatory synapses through the recruitment of pre-and-postsynaptic proteins. Organize the lamina/pathway-specific differentiation of dendrites. Plays a important role for auditory synaptic responses. Involved in the suppression of glioma.3 Publications

GO - Molecular functioni

GO - Biological processi

  • cytokine-mediated signaling pathway Source: GO_Central
  • negative regulation of JAK-STAT cascade Source: GO_Central
  • negative regulation of protein kinase activity Source: GO_Central
  • postsynaptic density protein 95 clustering Source: MGI
  • regulation of synapse organization Source: MGI
  • synapse organization Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat-containing protein 4
Alternative name(s):
Brain tumor-associated protein MBAG1
Netrin-G2 ligand
Short name:
NGL-2
Gene namesi
Name:Lrrc4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2182081. Lrrc4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini41 – 526ExtracellularSequence analysisAdd BLAST486
Transmembranei527 – 547HelicalSequence analysisAdd BLAST21
Topological domaini548 – 652CytoplasmicSequence analysisAdd BLAST105

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: GO_Central
  • dendritic spine Source: MGI
  • excitatory synapse Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • neuron spine Source: MGI
  • postsynaptic membrane Source: MGI
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mutant mice dysplay impaired startle response to acoustic stimulus.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 40Sequence analysisAdd BLAST40
ChainiPRO_000001483441 – 652Leucine-rich repeat-containing protein 4Add BLAST612

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 51PROSITE-ProRule annotation
Disulfide bondi49 ↔ 60PROSITE-ProRule annotation
Glycosylationi276N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi303 ↔ 328PROSITE-ProRule annotation
Disulfide bondi305 ↔ 349PROSITE-ProRule annotation
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi373 ↔ 423PROSITE-ProRule annotation
Glycosylationi387N-linked (GlcNAc...)Sequence analysis1
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Glycosylationi449N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ99PH1.
PRIDEiQ99PH1.

PTM databases

PhosphoSitePlusiQ99PH1.

Expressioni

Tissue specificityi

Specifically expressed in brain. In the hippocampus, parietal cortex and piriform cortex expressed in proximal segments of CA1 pyramidal neurons.2 Publications

Gene expression databases

BgeeiENSMUSG00000049939.
CleanExiMM_LRRC4.
ExpressionAtlasiQ99PH1. baseline and differential.
GenevisibleiQ99PH1. MM.

Interactioni

Subunit structurei

Interacts (via LRR repeats) with NTNG2. Interacts with DLG4. Forms a complex with DLG4 and with NMDA receptors.2 Publications

Protein-protein interaction databases

DIPiDIP-46449N.
STRINGi10090.ENSMUSP00000062158.

Structurei

3D structure databases

ProteinModelPortaliQ99PH1.
SMRiQ99PH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 74LRRNTAdd BLAST34
Repeati75 – 96LRR 1Add BLAST22
Repeati99 – 120LRR 2Add BLAST22
Repeati123 – 144LRR 3Add BLAST22
Repeati147 – 168LRR 4Add BLAST22
Repeati171 – 193LRR 5Add BLAST23
Repeati196 – 217LRR 6Add BLAST22
Repeati218 – 239LRR 7Add BLAST22
Repeati242 – 263LRR 8Add BLAST22
Repeati266 – 287LRR 9Add BLAST22
Domaini299 – 351LRRCTAdd BLAST53
Domaini352 – 441Ig-likeAdd BLAST90

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi454 – 525Thr-richAdd BLAST72

Domaini

The last 4 C-terminal residues bind to the first 2 PDZ domains of DLG4.

Sequence similaritiesi

Contains 9 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000252924.
HOVERGENiHBG052359.
InParanoidiQ99PH1.
KOiK16351.
OMAiNISEDRM.
OrthoDBiEOG091G037N.
PhylomeDBiQ99PH1.
TreeFamiTF324303.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026882. Lrrc4.
IPR000372. LRRNT.
[Graphical view]
PANTHERiPTHR24369:SF9. PTHR24369:SF9. 2 hits.
PfamiPF07679. I-set. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 7 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLWQVTVH HTWNAVLLPV VYLTAQVWIL CAAIAAAASA GPQNCPSVCS
60 70 80 90 100
CSNQFSKVVC TRRGLSEVPQ GIPSNTRYLN LMENNIQMIQ ADTFRHLHHL
110 120 130 140 150
EVLQLGRNSI RQIEVGAFNG LASLNTLELF DNWLTVIPSG AFEYLSKLRE
160 170 180 190 200
LWLRNNPIES IPSYAFNRVP SLMRLDLGEL KKLEYISEGA FEGLFNLKYL
210 220 230 240 250
NLGMCNIKDM PNLTPLVGLE ELEMSGNHFP EIRPGSFHGL SSLKKLWVMN
260 270 280 290 300
SQVSLIERNA FDGLASLVEL NLAHNNLSSL PHDLFTPLRY LVELHLHHNP
310 320 330 340 350
WNCDCDILWL AWWLREYIPT NSTCCGRCHA PMHMRGRYLV EVDQAAFQCS
360 370 380 390 400
APFIMDAPRD LNISEDRMAE LKCRTPPMSS VKWLLPNGTV LSHASRHPRI
410 420 430 440 450
SVLNDGTLNF SRVLLIDTGV YTCMVTNVAG NSNASAYLNV SSAELNTPNF
460 470 480 490 500
SFFTTVTVET TEISPEDITR KYKPVPTTST GYQPAYTTST TVLIQTTRVP
510 520 530 540 550
KQVPVPSTDT TDKMQTSLDE VMKTTKIIIG CFVAVTLLAA AMLIVFYKLR
560 570 580 590 600
KRHQQRSTVT AARTVEIIQV DEDIPAAAPA AATAAPSGVS GEGAVVLPTI
610 620 630 640 650
HDHINYNTYK PAHGAHWTEN SLGNSLHPTV TTISEPYIIQ THTKDKVQET

QI
Length:652
Mass (Da):72,619
Last modified:October 1, 2002 - v2
Checksum:i9D2807A1AEE30062
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252 – 256QVSLI → H in AAL67671 (Ref. 1) Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300458 mRNA. Translation: AAL67671.1.
DQ177325 mRNA. Translation: AAG60620.2.
BC117834 mRNA. Translation: AAI17835.1.
CCDSiCCDS39448.1.
RefSeqiNP_619623.2. NM_138682.2.
UniGeneiMm.443660.

Genome annotation databases

EnsembliENSMUST00000062304; ENSMUSP00000062158; ENSMUSG00000049939.
GeneIDi192198.
KEGGimmu:192198.
UCSCiuc009bcu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300458 mRNA. Translation: AAL67671.1.
DQ177325 mRNA. Translation: AAG60620.2.
BC117834 mRNA. Translation: AAI17835.1.
CCDSiCCDS39448.1.
RefSeqiNP_619623.2. NM_138682.2.
UniGeneiMm.443660.

3D structure databases

ProteinModelPortaliQ99PH1.
SMRiQ99PH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46449N.
STRINGi10090.ENSMUSP00000062158.

PTM databases

PhosphoSitePlusiQ99PH1.

Proteomic databases

PaxDbiQ99PH1.
PRIDEiQ99PH1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062304; ENSMUSP00000062158; ENSMUSG00000049939.
GeneIDi192198.
KEGGimmu:192198.
UCSCiuc009bcu.1. mouse.

Organism-specific databases

CTDi64101.
MGIiMGI:2182081. Lrrc4.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000118831.
HOGENOMiHOG000252924.
HOVERGENiHBG052359.
InParanoidiQ99PH1.
KOiK16351.
OMAiNISEDRM.
OrthoDBiEOG091G037N.
PhylomeDBiQ99PH1.
TreeFamiTF324303.

Miscellaneous databases

PROiQ99PH1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049939.
CleanExiMM_LRRC4.
ExpressionAtlasiQ99PH1. baseline and differential.
GenevisibleiQ99PH1. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR026882. Lrrc4.
IPR000372. LRRNT.
[Graphical view]
PANTHERiPTHR24369:SF9. PTHR24369:SF9. 2 hits.
PfamiPF07679. I-set. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 7 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRRC4_MOUSE
AccessioniPrimary (citable) accession number: Q99PH1
Secondary accession number(s): Q149E5, Q8VI35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.