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Q99PG6 (TS1R1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 1 member 1
Alternative name(s):
G-protein coupled receptor 70
Gene names
Name:Tas1r1
Synonyms:Gpr70, T1r1, Tr1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length842 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. Ref.5

Subunit structure

Forms heterodimers with TAS1R3.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed strongly only in fungiform papillae. Ref.3

Sequence similarities

Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.

Ontologies

Keywords
   Biological processSensory transduction
Taste
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processsensory perception of taste

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 842823Taste receptor type 1 member 1
PRO_0000012955

Regions

Topological domain20 – 568549Extracellular Potential
Transmembrane569 – 58921Helical; Name=1; Potential
Topological domain590 – 60415Cytoplasmic Potential
Transmembrane605 – 62521Helical; Name=2; Potential
Topological domain626 – 64015Extracellular Potential
Transmembrane641 – 66121Helical; Name=3; Potential
Topological domain662 – 68120Cytoplasmic Potential
Transmembrane682 – 70221Helical; Name=4; Potential
Topological domain703 – 72523Extracellular Potential
Transmembrane726 – 74621Helical; Name=5; Potential
Topological domain747 – 76216Cytoplasmic Potential
Transmembrane763 – 78321Helical; Name=6; Potential
Topological domain784 – 7896Extracellular Potential
Transmembrane790 – 81021Helical; Name=7; Potential
Topological domain811 – 84232Cytoplasmic Potential

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1361N-linked (GlcNAc...) Potential
Glycosylation2921N-linked (GlcNAc...) Potential
Glycosylation4801N-linked (GlcNAc...) Potential
Glycosylation5301N-linked (GlcNAc...) Potential

Natural variations

Natural variant2441N → D in strain: 129/J. Ref.2
Natural variant2811D → A in strain: 129/J. Ref.2
Natural variant309 – 3146NIPNVS → YITNVP in strain: 129/J.
Natural variant3471M → T in strain: 129/J. Ref.2
Natural variant4181H → Y in strain: 129/J. Ref.2
Natural variant4431K → N in strain: 129/J. Ref.2
Natural variant6261K → E in strain: 129/J. Ref.2

Experimental info

Sequence conflict1201L → P in AAK51603. Ref.3
Sequence conflict6481S → P Ref.4
Sequence conflict6491C → F Ref.4
Sequence conflict6551F → S Ref.4
Sequence conflict6591I → V Ref.4
Sequence conflict7761A → V Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q99PG6 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 58826C43F5DD352E

FASTA84293,426
        10         20         30         40         50         60 
MLFWAAHLLL SLQLAVAYCW AFSCQRTESS PGFSLPGDFL LAGLFSLHAD CLQVRHRPLV 

        70         80         90        100        110        120 
TSCDRSDSFN GHGYHLFQAM RFTVEEINNS TALLPNITLG YELYDVCSES SNVYATLRVL 

       130        140        150        160        170        180 
AQQGTGHLEM QRDLRNHSSK VVALIGPDNT DHAVTTAALL SPFLMPLVSY EASSVILSGK 

       190        200        210        220        230        240 
RKFPSFLRTI PSDKYQVEVI VRLLQSFGWV WISLVGSYGD YGQLGVQALE ELATPRGICV 

       250        260        270        280        290        300 
AFKNVVPLSA QAGDPRMQRM MLRLARARTT VVVVFSNRHL DGVFFRSVVL ANLTGKVWIA 

       310        320        330        340        350        360 
SEDWAISTNI PNVSGIQGIG TVLGVAIQQR QVPGLKEFEE SYVQAVMGAP RTCPEGSWCG 

       370        380        390        400        410        420 
TNQLCRECHA FTTWNMPELG AFSMSAAYNV YEAVYAVAHG LHQLLGCTSG TCARGPVHPW 

       430        440        450        460        470        480 
QLLQQIYKVN FLLHKKTVAF DDKGDPLGYY DIIAWDWNGP EWTFEVIGSA SLSPVHLDIN 

       490        500        510        520        530        540 
KTKIQWHGKN NQVPVSVCTR DCLEGHHRLV MGSHHCCFEC MPCEAGTFLN TSELHTCQPC 

       550        560        570        580        590        600 
GTEEWAPEGS SACFSRTVEF LGWHEPISLV LLAANTLLLL LLIGTAGLFA WRLHTPVVRS 

       610        620        630        640        650        660 
AGGRLCFLML GSLVAGSCSL YSFFGKPTVP ACLLRQPLFS LGFAIFLSCL TIRSFQLVII 

       670        680        690        700        710        720 
FKFSTKVPTF YHTWAQNHGA GIFVIVSSTV HLFLCLTWLA MWTPRPTREY QRFPHLVILE 

       730        740        750        760        770        780 
CTEVNSVGFL VAFAHNILLS ISTFVCSYLG KELPENYNEA KCVTFSLLLH FVSWIAFFTM 

       790        800        810        820        830        840 
SSIYQGSYLP AVNVLAGLAT LSGGFSGYFL PKCYVILCRP ELNNTEHFQA SIQDYTRRCG 


TT

« Hide

References

[1]"Mammalian sweet taste receptors."
Nelson G., Hoon M.A., Chandrashekar J., Zhang Y., Ryba N.J.P., Zuker C.S.
Cell 106:381-390(2001) [PubMed: 11509186] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal chromosome 4."
Li X., Inoue M., Reed D.R., Huque T., Puchalski R.B., Tordoff M.G., Ninomiya Y., Beauchamp G.K., Bachmanov A.A.
Mamm. Genome 12:13-16(2001) [PubMed: 11178737] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASP-244; ALA-281; 309-TYR--PRO-314; THR-347; TYR-418; ASN-443 AND GLU-626.
Strain: 129/J and C57BL/6ByJ.
[3]"A candidate taste receptor gene near a sweet taste locus."
Montmayeur J.-P., Liberles S.D., Matsunami H., Buck L.B.
Nat. Neurosci. 4:492-498(2001) [PubMed: 11319557] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6J.
Tissue: Circumvallate papilla and Foliate papilla.
[4]"Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose."
Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M., Kurimoto M.
In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003) [PubMed: 12892531] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[5]"An amino-acid taste receptor."
Nelson G., Chandrashekar J., Hoon M.A., Feng L., Zhao G., Ryba N.J.P., Zuker C.S.
Nature 416:199-202(2002) [PubMed: 11894099] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY032622 mRNA. Translation: AAK51603.1.
AF301161 mRNA. Translation: AAK07091.1.
AF301162 mRNA. Translation: AAK07092.1.
AF337040 mRNA. Translation: AAK39437.1.
IPIIPI00462902.
RefSeqNP_114073.1. NM_031867.2.
UniGeneMm.29232.

3D structure databases

ProteinModelPortalQ99PG6.
SMRQ99PG6. Positions 31-558.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ99PG6.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PRIDEQ99PG6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID110326.
KEGGmmu:110326.

Organism-specific databases

CTD80835.
MGIMGI:1927505. Tas1r1.

Phylogenomic databases

GeneTreeENSGT00580000081472.
HOGENOMHBG446995.
HOVERGENHBG108601.
InParanoidQ99PG6.
OrthoDBEOG4NKBTW.

Gene expression databases

ArrayExpressQ99PG6.
BgeeQ99PG6.
CleanExMM_TAS1R1.
GenevestigatorQ99PG6.
GermOnlineENSMUSG00000028950. Mus musculus.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
[Graphical view]
KOK04624.
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. False negative.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio363783.
SOURCESearch...

Entry information

Entry nameTS1R1_MOUSE
AccessionPrimary (citable) accession number: Q99PG6
Secondary accession number(s): Q6NS58 expand/collapse secondary AC list , Q923J9, Q925I5, Q99PG5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2001
Last modified: November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents