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Protein

Far upstream element-binding protein 2

Gene

Khsrp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs (By similarity). Binds to the dendritic targeting element and may play a role in mRNA trafficking.By similarity1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • mRNA 3'-UTR AU-rich region binding Source: UniProtKB
  • mRNA 3'-UTR binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Far upstream element-binding protein 2
Short name:
FUSE-binding protein 2
Alternative name(s):
KH type-splicing regulatory protein
Short name:
KSRP
MAP2 RNA trans-acting protein 1
Short name:
MARTA1
Gene namesi
Name:Khsrp
Synonyms:Fubp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621828. Khsrp.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • dendrite Source: RGD
  • neuronal cell body Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000501382 – 721Far upstream element-binding protein 2Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei40Omega-N-methylarginineBy similarity1
Modified residuei88N6-acetyllysineBy similarity1
Modified residuei101PhosphothreonineBy similarity1
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei126PhosphoserineBy similarity1
Modified residuei130PhosphoserineBy similarity1
Modified residuei182PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei194PhosphoserineBy similarity1
Modified residuei275PhosphoserineBy similarity1
Modified residuei412Omega-N-methylarginineBy similarity1
Modified residuei414Omega-N-methylarginineBy similarity1
Modified residuei416Omega-N-methylarginineBy similarity1
Modified residuei443Omega-N-methylarginineBy similarity1
Modified residuei481PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ99PF5.
PRIDEiQ99PF5.

2D gel databases

REPRODUCTION-2DPAGEQ99PF5.

PTM databases

iPTMnetiQ99PF5.
PhosphoSitePlusiQ99PF5.

Interactioni

Subunit structurei

Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRPH1. Interacts with PARN (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066023.

Structurei

3D structure databases

ProteinModelPortaliQ99PF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 209KH 1PROSITE-ProRule annotationAdd BLAST65
Domaini234 – 300KH 2PROSITE-ProRule annotationAdd BLAST67
Domaini323 – 387KH 3PROSITE-ProRule annotationAdd BLAST65
Domaini425 – 492KH 4PROSITE-ProRule annotationAdd BLAST68
Repeati572 – 5831Add BLAST12
Repeati618 – 6292Add BLAST12
Repeati644 – 6553Add BLAST12
Repeati674 – 6854Add BLAST12

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni572 – 6854 X 12 AA imperfect repeatsAdd BLAST114

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 68Gly/Pro-richAdd BLAST62
Compositional biasi69 – 498Gly-richAdd BLAST430
Compositional biasi499 – 613Ala/Gly/Pro-richAdd BLAST115

Sequence similaritiesi

Belongs to the KHSRP family.Curated
Contains 4 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1676. Eukaryota.
ENOG410XZYE. LUCA.
HOVERGENiHBG000625.
InParanoidiQ99PF5.
KOiK13210.
PhylomeDBiQ99PF5.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
InterProiIPR015096. DUF1897.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF09005. DUF1897. 2 hits.
PF00013. KH_1. 4 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYSTGGPP PGPPPPAGGG GGAAGAGGGP PPGPPGAGDR GGGGPGGGGP
60 70 80 90 100
GGGGASGGPS QPPGGGGPGI RKDAFADAVQ RARQIAAKIG GDAATTVNNN
110 120 130 140 150
TPDFGFGGQK RQLEDGDQPD SKKLASQGDS IGSQLGPIHP PPRTSMTEEY
160 170 180 190 200
RVPDGMVGLI IGRGGEQINK IQQDSGCKVQ ISPDSGGLPE RSVSLTGAPE
210 220 230 240 250
SVQKAKMMLD DIVSRGRGGP PGQFHDNANG GQNGTVQEIM IPAGKAGLVI
260 270 280 290 300
GKGGETIKQL QERAGVKMIL IQDGSQNTNV DKPLRIIGDP YKVQQACEMV
310 320 330 340 350
MDILRERDQG GFGDRNEYGS RVGGGIDVPV PRHSVGVVIG RSGEMIKKIQ
360 370 380 390 400
NDAGVRIQFK QDDGTGPEKI AHIMGPPDRC EHAARIINDL LQSLRSGPPG
410 420 430 440 450
PPGAPGMPPG GRGRGRGQGN WGPPGGEMTF SIPTHKCGLV IGRGGENVKA
460 470 480 490 500
INQQTGAFVE ISRQLPPNGD PNFKLFVIRG SPQQIDHAKQ LIEEKIEGPL
510 520 530 540 550
CPVGPGPGGP GPAGPMGPFH PGPFNQGPPG APPHAGGPPP HQYPPQGWGN
560 570 580 590 600
TYPEWQPPAP HDPNKAAAAA TDPNAAWAAY YSHYYQQPPG PVPGPAPAPA
610 620 630 640 650
APPAQGEPPQ PPPTGQSDYT KAWEEYYKKI GQQPQQPGAP PQQDYTKAWE
660 670 680 690 700
EYYKKQAQVA TGGGPGAPPG SQPDYSAAWA EYYRQQAAYY GQTPGPGGPQ
710 720
PPSTQQGQQQ ATEANGYELH L
Length:721
Mass (Da):74,226
Last modified:June 1, 2001 - v1
Checksum:i482C7A765C60EE4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF308818 mRNA. Translation: AAG59811.1.
RefSeqiNP_598286.1. NM_133602.1.
UniGeneiRn.92643.

Genome annotation databases

GeneIDi171137.
KEGGirno:171137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF308818 mRNA. Translation: AAG59811.1.
RefSeqiNP_598286.1. NM_133602.1.
UniGeneiRn.92643.

3D structure databases

ProteinModelPortaliQ99PF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066023.

PTM databases

iPTMnetiQ99PF5.
PhosphoSitePlusiQ99PF5.

2D gel databases

REPRODUCTION-2DPAGEQ99PF5.

Proteomic databases

PaxDbiQ99PF5.
PRIDEiQ99PF5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171137.
KEGGirno:171137.

Organism-specific databases

CTDi8570.
RGDi621828. Khsrp.

Phylogenomic databases

eggNOGiKOG1676. Eukaryota.
ENOG410XZYE. LUCA.
HOVERGENiHBG000625.
InParanoidiQ99PF5.
KOiK13210.
PhylomeDBiQ99PF5.

Miscellaneous databases

PROiQ99PF5.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
InterProiIPR015096. DUF1897.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF09005. DUF1897. 2 hits.
PF00013. KH_1. 4 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUBP2_RAT
AccessioniPrimary (citable) accession number: Q99PF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.