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Protein

DNA-(apurinic or apyrimidinic site) lyase

Gene

Apex

Organism
Rattus norvegicus (Rat)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Probably binds two magnesium or manganese ions per subunit.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, EndonucleaseUniRule annotation, Hydrolase, LyaseUniRule annotation, Nuclease
Biological processDNA damage, DNA repairUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Gene namesi
Name:ApexImported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, MitochondrionUniRule annotation, NucleusUniRule annotation

Interactioni

Protein-protein interaction databases

IntActiQ99PF3, 1 interactor
MINTiQ99PF3

Structurei

3D structure databases

ProteinModelPortaliQ99PF3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 280Endo/exonuclease/phosphataseInterPro annotationAdd BLAST221

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.UniRule annotation

Phylogenomic databases

HOVERGENiHBG050531

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR004808 AP_endonuc_1
IPR020847 AP_endonuclease_F1_BS
IPR020848 AP_endonuclease_F1_CS
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PANTHERiPTHR22748 PTHR22748, 2 hits
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
TIGRFAMsiTIGR00633 xth, 2 hits
PROSITEiView protein in PROSITE
PS00726 AP_NUCLEASE_F1_1, 1 hit
PS00727 AP_NUCLEASE_F1_2, 1 hit
PS00728 AP_NUCLEASE_F1_3, 1 hit
PS51435 AP_NUCLEASE_F1_4, 1 hit

Sequencei

Sequence statusi: Fragment.

Q99PF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
KRAAAEDGEE PKSEPETKKS KGAAKKTEKE AAGEGPVLYE DPPDQKTSAS
60 70 80 90 100
GKSATLKICS WNVDGLRAWI KKKGLDWVKE EAPDILCLQE TKCSENKLPA
110 120 130 140 150
ELQELPGLTH QYWSAPSDKE GYSGVGLLSR QCPLKVSYGI AYVPNAGRGL
160 170 180 190 200
VRLEYRQRWD EAFRKFLKDL ASRKPLVLCG DLNVAHEEID LRNPKGNKKN
210 220 230 240 250
AGFTPQERQG FGEMLQAVPL ADSFRHLYPN TAYAYTFWTY MMNARSKNVG
260 270 280
WRLDYFLLSH SLLPALCDSK IRSKALGSDH CPITLYLAL
Length:289
Mass (Da):32,353
Last modified:June 1, 2001 - v1
Checksum:i67E82454D062CE51
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1Imported1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311054 mRNA Translation: AAG49922.1
UniGeneiRn.5949

Similar proteinsi

Entry informationi

Entry nameiQ99PF3_RAT
AccessioniPrimary (citable) accession number: Q99PF3
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2001
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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