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Q99PE8 (ABCG5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-binding cassette sub-family G member 5
Alternative name(s):
Sterolin-1
Gene names
Name:Abcg5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length652 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

Subunit structure

May form heterodimers with ABCG8 or be tightly coupled to ABCG8 along a pathway regulating diatery-sterol absorption and excretion By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed in the intestine and, at lower level, in the liver. Ref.2

Induction

Up-regulated by cholesterol feeding. Possibly mediated by the liver X receptor/retinoic X receptor (LXR/RXR) pathway. Endotoxin (LPS) significantly decreased mRNA levels in the liver but not in the small intestine. Ref.2 Ref.3

Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. [View classification]

Contains 1 ABC transmembrane type-2 domain.

Contains 1 ABC transporter domain.

Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcholesterol efflux

Inferred from mutant phenotype PubMed 14504269. Source: BHF-UCL

cholesterol homeostasis

Inferred from electronic annotation. Source: Compara

excretion

Inferred from mutant phenotype PubMed 14504269. Source: BHF-UCL

intestinal cholesterol absorption

Inferred by curator PubMed 12208868. Source: BHF-UCL

negative regulation of intestinal cholesterol absorption

Inferred from electronic annotation. Source: Compara

negative regulation of intestinal phytosterol absorption

Inferred from electronic annotation. Source: Compara

response to drug

Inferred from electronic annotation. Source: Compara

response to ionizing radiation

Inferred from electronic annotation. Source: Compara

response to nutrient

Inferred from electronic annotation. Source: Compara

   Cellular_componentapical plasma membrane

Inferred from mutant phenotype PubMed 14504269. Source: BHF-UCL

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATPase activity

Inferred from electronic annotation. Source: Compara

cholesterol transporter activity

Inferred from electronic annotation. Source: Compara

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 652652ATP-binding cassette sub-family G member 5
PRO_0000093394

Regions

Topological domain1 – 385385Cytoplasmic Potential
Transmembrane386 – 40621Helical; Name=1; Potential
Topological domain407 – 42216Extracellular Potential
Transmembrane423 – 44321Helical; Name=2; Potential
Topological domain444 – 46320Cytoplasmic Potential
Transmembrane464 – 48421Helical; Name=3; Potential
Topological domain485 – 50420Extracellular Potential
Transmembrane505 – 52521Helical; Name=4; Potential
Topological domain526 – 5294Cytoplasmic Potential
Transmembrane530 – 55021Helical; Name=5; Potential
Topological domain551 – 62272Extracellular Potential
Transmembrane623 – 64321Helical; Name=6; Potential
Topological domain644 – 6529Cytoplasmic Potential
Domain39 – 294256ABC transporter
Domain389 – 646258ABC transmembrane type-2
Nucleotide binding87 – 948ATP Potential

Amino acid modifications

Modified residue211Phosphoserine Ref.4
Glycosylation4101N-linked (GlcNAc...) Potential
Glycosylation5851N-linked (GlcNAc...) Potential
Glycosylation5921N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q99PE8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 80CE37ADCC19771E

FASTA65273,244
        10         20         30         40         50         60 
MGELPFLSPE GARGPHINRG SLSSLEQGSV TGTEARHSLG VLHVSYSVSN RVGPWWNIKS 

        70         80         90        100        110        120 
CQQKWDRQIL KDVSLYIESG QIMCILGSSG SGKTTLLDAI SGRLRRTGTL EGEVFVNGCE 

       130        140        150        160        170        180 
LRRDQFQDCF SYVLQSDVFL SSLTVRETLR YTAMLALCRS SADFYNKKVE AVMTELSLSH 

       190        200        210        220        230        240 
VADQMIGSYN FGGISSGERR RVSIAAQLLQ DPKVMMLDEP TTGLDCMTAN QIVLLLAELA 

       250        260        270        280        290        300 
RRDRIVIVTI HQPRSELFQH FDKIAILTYG ELVFCGTPEE MLGFFNNCGY PCPEHSNPFD 

       310        320        330        340        350        360 
FYMDLTSVDT QSREREIETY KRVQMLECAF KESDIYHKIL ENIERARYLK TLPTVPFKTK 

       370        380        390        400        410        420 
DPPGMFGKLG VLLRRVTRNL MRNKQAVIMR LVQNLIMGLF LIFYLLRVQN NTLKGAVQDR 

       430        440        450        460        470        480 
VGLLYQLVGA TPYTGMLNAV NLFPMLRAVS DQESQDGLYH KWQMLLAYVL HVLPFSVIAT 

       490        500        510        520        530        540 
VIFSSVCYWT LGLYPEVARF GYFSAALLAP HLIGEFLTLV LLGIVQNPNI VNSIVALLSI 

       550        560        570        580        590        600 
SGLLIGSGFI RNIQEMPIPL KILGYFTFQK YCCEILVVNE FYGLNFTCGG SNTSMLNHPM 

       610        620        630        640        650 
CAITQGVQFI EKTCPGATSR FTANFLILYG FIPALVILGI VIFKVRDYLI SR

« Hide

References

« Hide 'large scale' references
[1]"Identification of a gene, ABCG5, important in the regulation of dietary cholesterol absorption."
Lee M.-H., Lu K., Hazard S., Yu H., Shulenin S., Hidaka H., Kojima H., Allikmets R., Sakuma N., Pegoraro R., Srivastava A.K., Salen G., Dean M., Patel S.B.
Nat. Genet. 27:79-83(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Liver.
[2]"Accumulation of dietary cholesterol in sitosterolemia caused by mutations in adjacent ABC transporters."
Berge K.E., Tian H., Graf G.A., Yu L., Grishin N.V., Schultz J., Kwiterovich P., Shan B., Barnes R., Hobbs H.H.
Science 290:1771-1775(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[3]"Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR."
Khovidhunkit W., Moser A.H., Shigenaga J.K., Grunfeld C., Feingold K.R.
J. Lipid Res. 44:1728-1736(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DOWN-REGULATION BY ENDOTOXIN.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF312713 mRNA. Translation: AAG53097.1.
IPIIPI00120963.
RefSeqNP_114090.1. NM_031884.1.
UniGeneMm.289590.

3D structure databases

ProteinModelPortalQ99PE8.
SMRQ99PE8. Positions 43-345.
ModBaseSearch...

PTM databases

PhosphoSiteQ99PE8.

Proteomic databases

PRIDEQ99PE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000066175; ENSMUSP00000069495; ENSMUSG00000040505.
GeneID27409.
KEGGmmu:27409.
UCSCuc008dsz.1. mouse.

Organism-specific databases

CTD64240.
MGIMGI:1351659. Abcg5.

Phylogenomic databases

eggNOGCOG1131.
GeneTreeENSGT00700000104032.
HOGENOMHOG000033763.
HOVERGENHBG050443.
InParanoidQ99PE8.
KOK05683.
OMARNIQEMP.
OrthoDBEOG4X97GZ.

Gene expression databases

ArrayExpressQ99PE8.
BgeeQ99PE8.
GenevestigatorQ99PE8.
GermOnlineENSMUSG00000040505. Mus musculus.

Family and domain databases

InterProIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS51012. ABC_TM2. False negative.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio305422.
SOURCESearch...

Entry information

Entry nameABCG5_MOUSE
AccessionPrimary (citable) accession number: Q99PE8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: June 1, 2001
Last modified: May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents