Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-binding cassette sub-family G member 5

Gene

Abcg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi87 – 948ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cholesterol efflux Source: BHF-UCL
  • cholesterol homeostasis Source: MGI
  • excretion Source: BHF-UCL
  • intestinal cholesterol absorption Source: BHF-UCL
  • negative regulation of intestinal cholesterol absorption Source: MGI
  • negative regulation of intestinal phytosterol absorption Source: MGI
  • response to drug Source: Ensembl
  • response to ionizing radiation Source: Ensembl
  • response to nutrient Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1369062. ABC transporters in lipid homeostasis.
R-MMU-265473. Trafficking of dietary sterols.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family G member 5
Alternative name(s):
Sterolin-1
Gene namesi
Name:Abcg5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1351659. Abcg5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 385385CytoplasmicSequence analysisAdd
BLAST
Transmembranei386 – 40621Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini407 – 42216ExtracellularSequence analysisAdd
BLAST
Transmembranei423 – 44321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini444 – 46320CytoplasmicSequence analysisAdd
BLAST
Transmembranei464 – 48421Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini485 – 50420ExtracellularSequence analysisAdd
BLAST
Transmembranei505 – 52521Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini526 – 5294CytoplasmicSequence analysis
Transmembranei530 – 55021Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini551 – 62272ExtracellularSequence analysisAdd
BLAST
Transmembranei623 – 64321Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini644 – 6529CytoplasmicSequence analysis

GO - Cellular componenti

  • apical part of cell Source: MGI
  • apical plasma membrane Source: BHF-UCL
  • ATP-binding cassette (ABC) transporter complex Source: MGI
  • membrane Source: MGI
  • receptor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 652652ATP-binding cassette sub-family G member 5PRO_0000093394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi410 – 4101N-linked (GlcNAc...)Sequence analysis
Glycosylationi585 – 5851N-linked (GlcNAc...)Sequence analysis
Glycosylationi592 – 5921N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ99PE8.
PRIDEiQ99PE8.

PTM databases

iPTMnetiQ99PE8.
PhosphoSiteiQ99PE8.

Expressioni

Tissue specificityi

Expressed in the intestine and, at lower level, in the liver.1 Publication

Inductioni

Up-regulated by cholesterol feeding. Possibly mediated by the liver X receptor/retinoic X receptor (LXR/RXR) pathway. Endotoxin (LPS) significantly decreased mRNA levels in the liver but not in the small intestine.1 Publication

Gene expression databases

BgeeiQ99PE8.
ExpressionAtlasiQ99PE8. baseline and differential.
GenevisibleiQ99PE8. MM.

Interactioni

Subunit structurei

May form heterodimers with ABCG8 or be tightly coupled to ABCG8 along a pathway regulating diatery-sterol absorption and excretion.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069495.

Structurei

3D structure databases

ProteinModelPortaliQ99PE8.
SMRiQ99PE8. Positions 43-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 294256ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini389 – 646258ABC transmembrane type-2Add
BLAST

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000033763.
HOVERGENiHBG050443.
InParanoidiQ99PE8.
KOiK05683.
OMAiCELRRDQ.
OrthoDBiEOG7QNVKG.
PhylomeDBiQ99PE8.
TreeFamiTF105212.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99PE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGELPFLSPE GARGPHINRG SLSSLEQGSV TGTEARHSLG VLHVSYSVSN
60 70 80 90 100
RVGPWWNIKS CQQKWDRQIL KDVSLYIESG QIMCILGSSG SGKTTLLDAI
110 120 130 140 150
SGRLRRTGTL EGEVFVNGCE LRRDQFQDCF SYVLQSDVFL SSLTVRETLR
160 170 180 190 200
YTAMLALCRS SADFYNKKVE AVMTELSLSH VADQMIGSYN FGGISSGERR
210 220 230 240 250
RVSIAAQLLQ DPKVMMLDEP TTGLDCMTAN QIVLLLAELA RRDRIVIVTI
260 270 280 290 300
HQPRSELFQH FDKIAILTYG ELVFCGTPEE MLGFFNNCGY PCPEHSNPFD
310 320 330 340 350
FYMDLTSVDT QSREREIETY KRVQMLECAF KESDIYHKIL ENIERARYLK
360 370 380 390 400
TLPTVPFKTK DPPGMFGKLG VLLRRVTRNL MRNKQAVIMR LVQNLIMGLF
410 420 430 440 450
LIFYLLRVQN NTLKGAVQDR VGLLYQLVGA TPYTGMLNAV NLFPMLRAVS
460 470 480 490 500
DQESQDGLYH KWQMLLAYVL HVLPFSVIAT VIFSSVCYWT LGLYPEVARF
510 520 530 540 550
GYFSAALLAP HLIGEFLTLV LLGIVQNPNI VNSIVALLSI SGLLIGSGFI
560 570 580 590 600
RNIQEMPIPL KILGYFTFQK YCCEILVVNE FYGLNFTCGG SNTSMLNHPM
610 620 630 640 650
CAITQGVQFI EKTCPGATSR FTANFLILYG FIPALVILGI VIFKVRDYLI

SR
Length:652
Mass (Da):73,244
Last modified:June 1, 2001 - v1
Checksum:i80CE37ADCC19771E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312713 mRNA. Translation: AAG53097.1.
CCDSiCCDS29001.1.
RefSeqiNP_114090.1. NM_031884.2.
UniGeneiMm.289590.

Genome annotation databases

EnsembliENSMUST00000066175; ENSMUSP00000069495; ENSMUSG00000040505.
GeneIDi27409.
KEGGimmu:27409.
UCSCiuc008dsz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312713 mRNA. Translation: AAG53097.1.
CCDSiCCDS29001.1.
RefSeqiNP_114090.1. NM_031884.2.
UniGeneiMm.289590.

3D structure databases

ProteinModelPortaliQ99PE8.
SMRiQ99PE8. Positions 43-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069495.

PTM databases

iPTMnetiQ99PE8.
PhosphoSiteiQ99PE8.

Proteomic databases

PaxDbiQ99PE8.
PRIDEiQ99PE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066175; ENSMUSP00000069495; ENSMUSG00000040505.
GeneIDi27409.
KEGGimmu:27409.
UCSCiuc008dsz.1. mouse.

Organism-specific databases

CTDi64240.
MGIiMGI:1351659. Abcg5.

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000033763.
HOVERGENiHBG050443.
InParanoidiQ99PE8.
KOiK05683.
OMAiCELRRDQ.
OrthoDBiEOG7QNVKG.
PhylomeDBiQ99PE8.
TreeFamiTF105212.

Enzyme and pathway databases

ReactomeiR-MMU-1369062. ABC transporters in lipid homeostasis.
R-MMU-265473. Trafficking of dietary sterols.

Miscellaneous databases

NextBioi305422.
PROiQ99PE8.
SOURCEiSearch...

Gene expression databases

BgeeiQ99PE8.
ExpressionAtlasiQ99PE8. baseline and differential.
GenevisibleiQ99PE8. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a gene, ABCG5, important in the regulation of dietary cholesterol absorption."
    Lee M.-H., Lu K., Hazard S., Yu H., Shulenin S., Hidaka H., Kojima H., Allikmets R., Sakuma N., Pegoraro R., Srivastava A.K., Salen G., Dean M., Patel S.B.
    Nat. Genet. 27:79-83(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  2. "Accumulation of dietary cholesterol in sitosterolemia caused by mutations in adjacent ABC transporters."
    Berge K.E., Tian H., Graf G.A., Yu L., Grishin N.V., Schultz J., Kwiterovich P., Shan B., Barnes R., Hobbs H.H.
    Science 290:1771-1775(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  3. "Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR."
    Khovidhunkit W., Moser A.H., Shigenaga J.K., Grunfeld C., Feingold K.R.
    J. Lipid Res. 44:1728-1736(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOWN-REGULATION BY ENDOTOXIN.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiABCG5_MOUSE
AccessioniPrimary (citable) accession number: Q99PE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.