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Protein

Sodium/potassium/calcium exchanger 3

Gene

Slc24a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transports 1 Ca2+ and 1 K+ in exchange for 4 Na+.By similarity

GO - Molecular functioni

  • calcium, potassium:sodium antiporter activity Source: MGI
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • cellular calcium ion homeostasis Source: MGI
  • sodium ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Antiport, Calcium transport, Ion transport, Potassium transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Calcium, Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-425561. Sodium/Calcium exchangers.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium/calcium exchanger 3
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 3
Solute carrier family 24 member 3
Gene namesi
Name:Slc24a3
Synonyms:Nckx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2137513. Slc24a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini44 – 10663ExtracellularSequence analysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence analysisAdd
BLAST
Topological domaini128 – 15124CytoplasmicSequence analysisAdd
BLAST
Transmembranei152 – 17221HelicalSequence analysisAdd
BLAST
Topological domaini173 – 1819ExtracellularSequence analysis
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Topological domaini203 – 2097CytoplasmicSequence analysis
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Topological domaini231 – 2344ExtracellularSequence analysis
Transmembranei235 – 25521HelicalSequence analysisAdd
BLAST
Topological domaini256 – 486231CytoplasmicSequence analysisAdd
BLAST
Transmembranei487 – 50721HelicalSequence analysisAdd
BLAST
Topological domaini508 – 5125ExtracellularSequence analysis
Transmembranei513 – 53321HelicalSequence analysisAdd
BLAST
Topological domaini534 – 55118CytoplasmicSequence analysisAdd
BLAST
Transmembranei552 – 57221HelicalSequence analysisAdd
BLAST
Topological domaini573 – 58210ExtracellularSequence analysis
Transmembranei583 – 60321HelicalSequence analysisAdd
BLAST
Topological domaini604 – 61714CytoplasmicSequence analysisAdd
BLAST
Transmembranei618 – 63821HelicalSequence analysisAdd
BLAST
Topological domaini639 – 6457ExtracellularSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4343Sequence analysisAdd
BLAST
Chaini44 – 645602Sodium/potassium/calcium exchanger 3PRO_0000019371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi70 – 701N-linked (GlcNAc...)Sequence analysis
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence analysis
Modified residuei307 – 3071PhosphoserineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ99PD7.
PaxDbiQ99PD7.
PRIDEiQ99PD7.

PTM databases

iPTMnetiQ99PD7.
PhosphoSiteiQ99PD7.

Expressioni

Tissue specificityi

Abundant in the brain. Highest levels found in selected thalamic nuclei, hippocampal CA1 neurons and in layer IV of the cerebral cortex. Expressed in dental tissues.1 Publication

Gene expression databases

BgeeiQ99PD7.
ExpressionAtlasiQ99PD7. baseline and differential.
GenevisibleiQ99PD7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079897.

Structurei

3D structure databases

ProteinModelPortaliQ99PD7.
SMRiQ99PD7. Positions 129-251, 503-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati148 – 18841Alpha-1Add
BLAST
Repeati520 – 55132Alpha-2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi424 – 4307Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG054881.
InParanoidiQ99PD7.
OMAiTVFGVHM.
OrthoDBiEOG776SP8.
TreeFamiTF318759.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR030248. SLC24A3.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 1 hit.
PTHR10846:SF22. PTHR10846:SF22. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00367. TIGR00367. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99PD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPPGDQDCA RRRSRRRRRD LLLSQLCFLA SVALLLWSLS SLREQKELDL
60 70 80 90 100
MDLIGEDRKW MVGRKLMQVN DTLTSEDAGL QSSKNCTEPA LHEFPRDIFS
110 120 130 140 150
NEDRRQGAVV LHVLCAMYMF YALAIVCDDF FVPSLEKICE RLHLSEDVAG
160 170 180 190 200
ATFMAAGSSA PELFTSVIGV FITKGDVGVG TIVGSAVFNI LCIIGVCGLF
210 220 230 240 250
AGQVVALSSW CLLRDSIYYT LSVVALIVFI YDEKVSWWES LVLVLMYLIY
260 270 280 290 300
IVIMKYNACI HQCFERRTKG AGNMVNGLAN NAEIDDSSNC DATVVLLKKA
310 320 330 340 350
NFHRKASVIM VDELLSAYPH QLSFSEAGLR IMITSHFPPK TRLSMASRML
360 370 380 390 400
INERQRLINS RAYTNGESEV AIKIPIKHTV ENGTGPSSAP DRGVNGTRRD
410 420 430 440 450
DIVAETDNET ENENEDNENN ENDEEEEEDE DDDEGPYTPF DPPSGKLETV
460 470 480 490 500
KWAFTWPLSF VLYFTVPNCN KPHWEKWFMV TFASSTLWIA AFSYMMVWMV
510 520 530 540 550
TIIGYTLGIP DVIMGITFLA AGTSVPDCMA SLIVARQGMG DMAVSNSIGS
560 570 580 590 600
NVFDILIGLG LPWALQTLAV DYGSYIRLNS RGLIYSVGLL LASVFVTVFG
610 620 630 640
VHLNKWQLDK KLGCGCLFLY GVFLCFSIMT EFNVFTFVNL PMCGD
Length:645
Mass (Da):71,942
Last modified:July 27, 2011 - v3
Checksum:iFA57D33BA1D14B84
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti422 – 4221N → S in AAG60049 (PubMed:11294880).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314821 mRNA. Translation: AAG60049.1.
AF314822 Genomic DNA. Translation: AAG60050.1.
AL772192
, AL808117, AL824715, AL831783 Genomic DNA. Translation: CAM15824.1.
AL831783
, AL772192, AL808117, AL824715 Genomic DNA. Translation: CAM17347.1.
AL808117
, AL772192, AL824715, AL831783 Genomic DNA. Translation: CAM18366.1.
AL824715
, AL772192, AL808117, AL831783 Genomic DNA. Translation: CAM27508.1.
BC005742 mRNA. Translation: AAH05742.1.
UniGeneiMm.217171.

Genome annotation databases

EnsembliENSMUST00000110007; ENSMUSP00000105634; ENSMUSG00000063873.
UCSCiuc008mrq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314821 mRNA. Translation: AAG60049.1.
AF314822 Genomic DNA. Translation: AAG60050.1.
AL772192
, AL808117, AL824715, AL831783 Genomic DNA. Translation: CAM15824.1.
AL831783
, AL772192, AL808117, AL824715 Genomic DNA. Translation: CAM17347.1.
AL808117
, AL772192, AL824715, AL831783 Genomic DNA. Translation: CAM18366.1.
AL824715
, AL772192, AL808117, AL831783 Genomic DNA. Translation: CAM27508.1.
BC005742 mRNA. Translation: AAH05742.1.
UniGeneiMm.217171.

3D structure databases

ProteinModelPortaliQ99PD7.
SMRiQ99PD7. Positions 129-251, 503-555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079897.

PTM databases

iPTMnetiQ99PD7.
PhosphoSiteiQ99PD7.

Proteomic databases

MaxQBiQ99PD7.
PaxDbiQ99PD7.
PRIDEiQ99PD7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110007; ENSMUSP00000105634; ENSMUSG00000063873.
UCSCiuc008mrq.1. mouse.

Organism-specific databases

MGIiMGI:2137513. Slc24a3.

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG054881.
InParanoidiQ99PD7.
OMAiTVFGVHM.
OrthoDBiEOG776SP8.
TreeFamiTF318759.

Enzyme and pathway databases

ReactomeiR-MMU-425561. Sodium/Calcium exchangers.

Miscellaneous databases

ChiTaRSiSlc24a3. mouse.
PROiQ99PD7.
SOURCEiSearch...

Gene expression databases

BgeeiQ99PD7.
ExpressionAtlasiQ99PD7. baseline and differential.
GenevisibleiQ99PD7. MM.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR030248. SLC24A3.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 1 hit.
PTHR10846:SF22. PTHR10846:SF22. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00367. TIGR00367. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a third member of the potassium-dependent sodium-calcium exchanger gene family, NCKX3."
    Kraev A., Quednau B.D., Leach S., Li X.-F., Dong H., Winkfein R., Perizzolo M., Cai X., Yang R., Philipson K.D., Lytton J.
    J. Biol. Chem. 276:23161-23172(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ and CD-1.
    Tissue: Brain and Embryonic stem cell.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 323-645.
    Tissue: Mammary tumor.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Lung, Spleen and Testis.
  5. "Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts."
    Hu P., Lacruz R.S., Smith C.E., Smith S.M., Kurtz I., Paine M.L.
    Cells Tissues Organs 196:501-509(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiNCKX3_MOUSE
AccessioniPrimary (citable) accession number: Q99PD7
Secondary accession number(s): B1AWL7, Q99JR2, Q99PD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.