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Protein

Transmembrane glycoprotein NMB

Gene

Gpnmb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could be a melanogenic enzyme.By similarity

GO - Molecular functioni

  • heparin binding Source: MGI
  • integrin binding Source: MGI
  • syndecan binding Source: MGI

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-8857538. PTK6 promotes HIF1A stabilization.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane glycoprotein NMB
Alternative name(s):
DC-HIL
Dendritic cell-associated transmembrane protein
Osteoactivin
Gene namesi
Name:Gpnmb
Synonyms:Dchil, Hgfin, Nmb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1934765. Gpnmb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 502ExtracellularSequence analysisAdd BLAST480
Transmembranei503 – 523HelicalSequence analysisAdd BLAST21
Topological domaini524 – 574CytoplasmicSequence analysisAdd BLAST51

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Involvement in diseasei

May promote bone metastatic phenotype when overexpressed in breast cancer cells.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002471023 – 574Transmembrane glycoprotein NMBAdd BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1
Glycosylationi134N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi249N-linked (GlcNAc...)Sequence analysis1
Glycosylationi275N-linked (GlcNAc...)Sequence analysis1
Glycosylationi296N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Glycosylationi306N-linked (GlcNAc...)Sequence analysis1
Glycosylationi312N-linked (GlcNAc...)Sequence analysis1
Glycosylationi463N-linked (GlcNAc...)Sequence analysis1
Glycosylationi471N-linked (GlcNAc...)Sequence analysis1
Modified residuei546PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ99P91.
PaxDbiQ99P91.
PRIDEiQ99P91.

PTM databases

iPTMnetiQ99P91.
PhosphoSitePlusiQ99P91.

Expressioni

Tissue specificityi

In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype.1 Publication

Gene expression databases

BgeeiENSMUSG00000029816.
CleanExiMM_GPNMB.
MM_NMB.
ExpressionAtlasiQ99P91. baseline and differential.
GenevisibleiQ99P91. MM.

Interactioni

GO - Molecular functioni

  • integrin binding Source: MGI
  • syndecan binding Source: MGI

Protein-protein interaction databases

IntActiQ99P91. 1 interactor.
STRINGi10090.ENSMUSP00000031840.

Structurei

3D structure databases

ProteinModelPortaliQ99P91.
SMRiQ99P91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini250 – 338PKDPROSITE-ProRule annotationAdd BLAST89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi558 – 560Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the PMEL/NMB family.Curated
Contains 1 PKD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE9P. Eukaryota.
ENOG4110Q2V. LUCA.
GeneTreeiENSGT00530000063573.
HOGENOMiHOG000252941.
HOVERGENiHBG051797.
InParanoidiQ99P91.
KOiK20732.
OMAiFPGNQEK.
OrthoDBiEOG091G072N.
TreeFamiTF334865.

Family and domain databases

Gene3Di2.60.40.670. 1 hit.
InterProiIPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamiPF00801. PKD. 1 hit.
[Graphical view]
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99P91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLCGVLGF LLLAAGLPLQ AAKRFRDVLG HEQYPDHMRE HNQLRGWSSD
60 70 80 90 100
ENEWDEHLYP VWRRGDGRWK DSWEGGRVQA VLTSDSPALV GSNITFVVNL
110 120 130 140 150
VFPRCQKEDA NGNIVYEKNC RNDLGLTSDL HVYNWTAGAD DGDWEDGTSR
160 170 180 190 200
SQHLRFPDRR PFPRPHGWKK WSFVYVFHTL GQYFQKLGRC SARVSINTVN
210 220 230 240 250
LTAGPQVMEV TVFRRYGRAY IPISKVKDVY VITDQIPVFV TMSQKNDRNL
260 270 280 290 300
SDEIFLRDLP IVFDVLIHDP SHFLNDSAIS YKWNFGDNTG LFVSNNHTLN
310 320 330 340 350
HTYVLNGTFN LNLTVQTAVP GPCPPPSPST PPPPSTPPSP PPSPLPTLST
360 370 380 390 400
PSPSLMPTGY KSMELSDISN ENCRINRYGY FRATITIVEG ILEVSIMQIA
410 420 430 440 450
DVPMPTPQPA NSLMDFTVTC KGATPMEACT IISDPTCQIA QNRVCSPVAV
460 470 480 490 500
DGLCLLSVRR AFNGSGTYCV NFTLGDDASL ALTSTLISIP GKDPDSPLRA
510 520 530 540 550
VNGVLISIGC LAVLVTMVTI LLYKKHKAYK PIGNCPRNTV KGKGLSVLLS
560 570
HAKAPFFRGD QEKDPLLQDK PRTL
Length:574
Mass (Da):63,676
Last modified:July 27, 2011 - v2
Checksum:iB6AE99DD5DB7C6D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36D → N in AAK14240 (PubMed:11114299).Curated1
Sequence conflicti36D → N in BAC32074 (PubMed:16141072).Curated1
Sequence conflicti36D → N in AAH26375 (PubMed:15489334).Curated1
Sequence conflicti333P → S in CAB65272 (PubMed:12638126).Curated1
Sequence conflicti339 – 341SPP → LPS in CAB65272 (PubMed:12638126).Curated3
Sequence conflicti498L → M in BAC32074 (PubMed:16141072).Curated1
Sequence conflicti541K → E in BAC32074 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322054 mRNA. Translation: AAK14240.1.
AJ251685 mRNA. Translation: CAB65272.1.
AK044764 mRNA. Translation: BAC32074.1.
AK076347 mRNA. Translation: BAC36306.1.
AK150739 mRNA. Translation: BAE29813.1.
AK154617 mRNA. Translation: BAE32716.1.
AK170506 mRNA. Translation: BAE41844.1.
AK171441 mRNA. Translation: BAE42454.1.
AK171465 mRNA. Translation: BAE42471.1.
AK171474 mRNA. Translation: BAE42479.1.
AK171517 mRNA. Translation: BAE42501.1.
BC026375 mRNA. Translation: AAH26375.1.
CCDSiCCDS20122.1.
RefSeqiNP_444340.3. NM_053110.4.
UniGeneiMm.302602.
Mm.335454.

Genome annotation databases

EnsembliENSMUST00000031840; ENSMUSP00000031840; ENSMUSG00000029816.
GeneIDi93695.
KEGGimmu:93695.
UCSCiuc012elo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322054 mRNA. Translation: AAK14240.1.
AJ251685 mRNA. Translation: CAB65272.1.
AK044764 mRNA. Translation: BAC32074.1.
AK076347 mRNA. Translation: BAC36306.1.
AK150739 mRNA. Translation: BAE29813.1.
AK154617 mRNA. Translation: BAE32716.1.
AK170506 mRNA. Translation: BAE41844.1.
AK171441 mRNA. Translation: BAE42454.1.
AK171465 mRNA. Translation: BAE42471.1.
AK171474 mRNA. Translation: BAE42479.1.
AK171517 mRNA. Translation: BAE42501.1.
BC026375 mRNA. Translation: AAH26375.1.
CCDSiCCDS20122.1.
RefSeqiNP_444340.3. NM_053110.4.
UniGeneiMm.302602.
Mm.335454.

3D structure databases

ProteinModelPortaliQ99P91.
SMRiQ99P91.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99P91. 1 interactor.
STRINGi10090.ENSMUSP00000031840.

PTM databases

iPTMnetiQ99P91.
PhosphoSitePlusiQ99P91.

Proteomic databases

MaxQBiQ99P91.
PaxDbiQ99P91.
PRIDEiQ99P91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031840; ENSMUSP00000031840; ENSMUSG00000029816.
GeneIDi93695.
KEGGimmu:93695.
UCSCiuc012elo.1. mouse.

Organism-specific databases

CTDi10457.
MGIiMGI:1934765. Gpnmb.

Phylogenomic databases

eggNOGiENOG410IE9P. Eukaryota.
ENOG4110Q2V. LUCA.
GeneTreeiENSGT00530000063573.
HOGENOMiHOG000252941.
HOVERGENiHBG051797.
InParanoidiQ99P91.
KOiK20732.
OMAiFPGNQEK.
OrthoDBiEOG091G072N.
TreeFamiTF334865.

Enzyme and pathway databases

ReactomeiR-MMU-8857538. PTK6 promotes HIF1A stabilization.

Miscellaneous databases

ChiTaRSiGpnmb. mouse.
PROiQ99P91.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029816.
CleanExiMM_GPNMB.
MM_NMB.
ExpressionAtlasiQ99P91. baseline and differential.
GenevisibleiQ99P91. MM.

Family and domain databases

Gene3Di2.60.40.670. 1 hit.
InterProiIPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamiPF00801. PKD. 1 hit.
[Graphical view]
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPNMB_MOUSE
AccessioniPrimary (citable) accession number: Q99P91
Secondary accession number(s): Q3U3R9
, Q8BVV9, Q8BXL4, Q9QXA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.