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Q99P91

- GPNMB_MOUSE

UniProt

Q99P91 - GPNMB_MOUSE

Protein

Transmembrane glycoprotein NMB

Gene

Gpnmb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Could be a melanogenic enzyme.By similarity

    GO - Molecular functioni

    1. heparin binding Source: MGI
    2. integrin binding Source: MGI

    GO - Biological processi

    1. bone mineralization Source: Ensembl
    2. cell adhesion Source: MGI
    3. osteoblast differentiation Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transmembrane glycoprotein NMB
    Alternative name(s):
    DC-HIL
    Dendritic cell-associated transmembrane protein
    Osteoactivin
    Gene namesi
    Name:Gpnmb
    Synonyms:Dchil, Hgfin, Nmb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1934765. Gpnmb.

    Subcellular locationi

    Cell membrane By similarity; Single-pass type I membrane protein By similarity. Endomembrane system 1 Publication; Single-pass type I membrane protein 1 Publication. Cytoplasmic vesicle membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Melanosome By similarity
    Note: Vesicular, endosomal like structures.

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: MGI
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. endomembrane system Source: UniProtKB-SubCell
    4. integral component of plasma membrane Source: MGI
    5. melanosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    May promote bone metastatic phenotype when overexpressed in breast cancer cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 574552Transmembrane glycoprotein NMBPRO_0000024710Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi275 – 2751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi463 – 4631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi471 – 4711N-linked (GlcNAc...)Sequence Analysis
    Modified residuei546 – 5461Phosphoserine1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ99P91.
    PRIDEiQ99P91.

    PTM databases

    PhosphoSiteiQ99P91.

    Expressioni

    Tissue specificityi

    In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype.1 Publication

    Gene expression databases

    ArrayExpressiQ99P91.
    BgeeiQ99P91.
    CleanExiMM_GPNMB.
    MM_NMB.
    GenevestigatoriQ99P91.

    Interactioni

    Protein-protein interaction databases

    IntActiQ99P91. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99P91.
    SMRiQ99P91. Positions 258-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 502480ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini524 – 57451CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei503 – 52321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini250 – 33889PKDPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi558 – 5603Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Belongs to the PMEL/NMB family.Curated
    Contains 1 PKD domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40272.
    GeneTreeiENSGT00530000063573.
    HOGENOMiHOG000252941.
    HOVERGENiHBG051797.
    InParanoidiQ3U3R9.
    OMAiHTYVLNG.
    OrthoDBiEOG7GXPB9.
    TreeFamiTF334865.

    Family and domain databases

    Gene3Di2.60.40.670. 1 hit.
    InterProiIPR022409. PKD/Chitinase_dom.
    IPR000601. PKD_dom.
    [Graphical view]
    PfamiPF00801. PKD. 1 hit.
    [Graphical view]
    SMARTiSM00089. PKD. 1 hit.
    [Graphical view]
    SUPFAMiSSF49299. SSF49299. 1 hit.
    PROSITEiPS50093. PKD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99P91-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESLCGVLGF LLLAAGLPLQ AAKRFRDVLG HEQYPDHMRE HNQLRGWSSD    50
    ENEWDEHLYP VWRRGDGRWK DSWEGGRVQA VLTSDSPALV GSNITFVVNL 100
    VFPRCQKEDA NGNIVYEKNC RNDLGLTSDL HVYNWTAGAD DGDWEDGTSR 150
    SQHLRFPDRR PFPRPHGWKK WSFVYVFHTL GQYFQKLGRC SARVSINTVN 200
    LTAGPQVMEV TVFRRYGRAY IPISKVKDVY VITDQIPVFV TMSQKNDRNL 250
    SDEIFLRDLP IVFDVLIHDP SHFLNDSAIS YKWNFGDNTG LFVSNNHTLN 300
    HTYVLNGTFN LNLTVQTAVP GPCPPPSPST PPPPSTPPSP PPSPLPTLST 350
    PSPSLMPTGY KSMELSDISN ENCRINRYGY FRATITIVEG ILEVSIMQIA 400
    DVPMPTPQPA NSLMDFTVTC KGATPMEACT IISDPTCQIA QNRVCSPVAV 450
    DGLCLLSVRR AFNGSGTYCV NFTLGDDASL ALTSTLISIP GKDPDSPLRA 500
    VNGVLISIGC LAVLVTMVTI LLYKKHKAYK PIGNCPRNTV KGKGLSVLLS 550
    HAKAPFFRGD QEKDPLLQDK PRTL 574
    Length:574
    Mass (Da):63,676
    Last modified:July 27, 2011 - v2
    Checksum:iB6AE99DD5DB7C6D0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361D → N in AAK14240. (PubMed:11114299)Curated
    Sequence conflicti36 – 361D → N in BAC32074. (PubMed:16141072)Curated
    Sequence conflicti36 – 361D → N in AAH26375. (PubMed:15489334)Curated
    Sequence conflicti333 – 3331P → S in CAB65272. (PubMed:12638126)Curated
    Sequence conflicti339 – 3413SPP → LPS in CAB65272. (PubMed:12638126)Curated
    Sequence conflicti498 – 4981L → M in BAC32074. (PubMed:16141072)Curated
    Sequence conflicti541 – 5411K → E in BAC32074. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF322054 mRNA. Translation: AAK14240.1.
    AJ251685 mRNA. Translation: CAB65272.1.
    AK044764 mRNA. Translation: BAC32074.1.
    AK076347 mRNA. Translation: BAC36306.1.
    AK150739 mRNA. Translation: BAE29813.1.
    AK154617 mRNA. Translation: BAE32716.1.
    AK170506 mRNA. Translation: BAE41844.1.
    AK171441 mRNA. Translation: BAE42454.1.
    AK171465 mRNA. Translation: BAE42471.1.
    AK171474 mRNA. Translation: BAE42479.1.
    AK171517 mRNA. Translation: BAE42501.1.
    BC026375 mRNA. Translation: AAH26375.1.
    CCDSiCCDS20122.1.
    RefSeqiNP_444340.3. NM_053110.4.
    UniGeneiMm.302602.

    Genome annotation databases

    EnsembliENSMUST00000031840; ENSMUSP00000031840; ENSMUSG00000029816.
    GeneIDi93695.
    KEGGimmu:93695.
    UCSCiuc012elo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF322054 mRNA. Translation: AAK14240.1 .
    AJ251685 mRNA. Translation: CAB65272.1 .
    AK044764 mRNA. Translation: BAC32074.1 .
    AK076347 mRNA. Translation: BAC36306.1 .
    AK150739 mRNA. Translation: BAE29813.1 .
    AK154617 mRNA. Translation: BAE32716.1 .
    AK170506 mRNA. Translation: BAE41844.1 .
    AK171441 mRNA. Translation: BAE42454.1 .
    AK171465 mRNA. Translation: BAE42471.1 .
    AK171474 mRNA. Translation: BAE42479.1 .
    AK171517 mRNA. Translation: BAE42501.1 .
    BC026375 mRNA. Translation: AAH26375.1 .
    CCDSi CCDS20122.1.
    RefSeqi NP_444340.3. NM_053110.4.
    UniGenei Mm.302602.

    3D structure databases

    ProteinModelPortali Q99P91.
    SMRi Q99P91. Positions 258-320.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99P91. 1 interaction.

    PTM databases

    PhosphoSitei Q99P91.

    Proteomic databases

    MaxQBi Q99P91.
    PRIDEi Q99P91.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031840 ; ENSMUSP00000031840 ; ENSMUSG00000029816 .
    GeneIDi 93695.
    KEGGi mmu:93695.
    UCSCi uc012elo.1. mouse.

    Organism-specific databases

    CTDi 10457.
    MGIi MGI:1934765. Gpnmb.

    Phylogenomic databases

    eggNOGi NOG40272.
    GeneTreei ENSGT00530000063573.
    HOGENOMi HOG000252941.
    HOVERGENi HBG051797.
    InParanoidi Q3U3R9.
    OMAi HTYVLNG.
    OrthoDBi EOG7GXPB9.
    TreeFami TF334865.

    Miscellaneous databases

    ChiTaRSi GPNMB. mouse.
    NextBioi 351449.
    PROi Q99P91.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99P91.
    Bgeei Q99P91.
    CleanExi MM_GPNMB.
    MM_NMB.
    Genevestigatori Q99P91.

    Family and domain databases

    Gene3Di 2.60.40.670. 1 hit.
    InterProi IPR022409. PKD/Chitinase_dom.
    IPR000601. PKD_dom.
    [Graphical view ]
    Pfami PF00801. PKD. 1 hit.
    [Graphical view ]
    SMARTi SM00089. PKD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49299. SSF49299. 1 hit.
    PROSITEi PS50093. PKD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a dendritic cell-associated transmembrane protein, DC-HIL, that promotes RGD-dependent adhesion of endothelial cells through recognition of heparan sulfate proteoglycans."
      Shikano S., Bonkobara M., Zukas P.K., Ariizumi K.
      J. Biol. Chem. 276:8125-8134(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "mRNA expression of the murine glycoprotein (transmembrane) nmb (Gpnmb) gene is linked to the developing retinal pigment epithelium and iris."
      Bachner D., Schroder D., Gross G.
      Gene Expr. Patterns 1:159-165(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Retina and Skin.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    5. Cited for: DISEASE.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGPNMB_MOUSE
    AccessioniPrimary (citable) accession number: Q99P91
    Secondary accession number(s): Q3U3R9
    , Q8BVV9, Q8BXL4, Q9QXA0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3