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Q99P91 (GPNMB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transmembrane glycoprotein NMB
Alternative name(s):
DC-HIL
Dendritic cell-associated transmembrane protein
Osteoactivin
Gene names
Name:Gpnmb
Synonyms:Dchil, Hgfin, Nmb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could be a melanogenic enzyme By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Endomembrane system; Single-pass type I membrane protein. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Melanosome By similarity. Note: Vesicular, endosomal like structures. Ref.2

Tissue specificity

In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype. Ref.2

Involvement in disease

May promote bone metastatic phenotype when overexpressed in breast cancer cells. Ref.5

Sequence similarities

Belongs to the PMEL/NMB family.

Contains 1 PKD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 574552Transmembrane glycoprotein NMB
PRO_0000024710

Regions

Topological domain23 – 502480Extracellular Potential
Transmembrane503 – 52321Helical; Potential
Topological domain524 – 57451Cytoplasmic Potential
Domain250 – 33889PKD
Motif558 – 5603Cell attachment site Potential

Amino acid modifications

Modified residue5461Phosphoserine Ref.6
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation1341N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2491N-linked (GlcNAc...) Potential
Glycosylation2751N-linked (GlcNAc...) Potential
Glycosylation2961N-linked (GlcNAc...) Potential
Glycosylation3001N-linked (GlcNAc...) Potential
Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation3121N-linked (GlcNAc...) Potential
Glycosylation4631N-linked (GlcNAc...) Potential
Glycosylation4711N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict361D → N in AAK14240. Ref.1
Sequence conflict361D → N in BAC32074. Ref.3
Sequence conflict361D → N in AAH26375. Ref.4
Sequence conflict3331P → S in CAB65272. Ref.2
Sequence conflict339 – 3413SPP → LPS in CAB65272. Ref.2
Sequence conflict4981L → M in BAC32074. Ref.3
Sequence conflict5411K → E in BAC32074. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q99P91 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: B6AE99DD5DB7C6D0

FASTA57463,676
        10         20         30         40         50         60 
MESLCGVLGF LLLAAGLPLQ AAKRFRDVLG HEQYPDHMRE HNQLRGWSSD ENEWDEHLYP 

        70         80         90        100        110        120 
VWRRGDGRWK DSWEGGRVQA VLTSDSPALV GSNITFVVNL VFPRCQKEDA NGNIVYEKNC 

       130        140        150        160        170        180 
RNDLGLTSDL HVYNWTAGAD DGDWEDGTSR SQHLRFPDRR PFPRPHGWKK WSFVYVFHTL 

       190        200        210        220        230        240 
GQYFQKLGRC SARVSINTVN LTAGPQVMEV TVFRRYGRAY IPISKVKDVY VITDQIPVFV 

       250        260        270        280        290        300 
TMSQKNDRNL SDEIFLRDLP IVFDVLIHDP SHFLNDSAIS YKWNFGDNTG LFVSNNHTLN 

       310        320        330        340        350        360 
HTYVLNGTFN LNLTVQTAVP GPCPPPSPST PPPPSTPPSP PPSPLPTLST PSPSLMPTGY 

       370        380        390        400        410        420 
KSMELSDISN ENCRINRYGY FRATITIVEG ILEVSIMQIA DVPMPTPQPA NSLMDFTVTC 

       430        440        450        460        470        480 
KGATPMEACT IISDPTCQIA QNRVCSPVAV DGLCLLSVRR AFNGSGTYCV NFTLGDDASL 

       490        500        510        520        530        540 
ALTSTLISIP GKDPDSPLRA VNGVLISIGC LAVLVTMVTI LLYKKHKAYK PIGNCPRNTV 

       550        560        570 
KGKGLSVLLS HAKAPFFRGD QEKDPLLQDK PRTL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a dendritic cell-associated transmembrane protein, DC-HIL, that promotes RGD-dependent adhesion of endothelial cells through recognition of heparan sulfate proteoglycans."
Shikano S., Bonkobara M., Zukas P.K., Ariizumi K.
J. Biol. Chem. 276:8125-8134(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"mRNA expression of the murine glycoprotein (transmembrane) nmb (Gpnmb) gene is linked to the developing retinal pigment epithelium and iris."
Bachner D., Schroder D., Gross G.
Gene Expr. Patterns 1:159-165(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Bone marrow, Retina and Skin.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[5]"Osteoactivin promotes breast cancer metastasis to bone."
Rose A.A., Pepin F., Russo C., Abou Khalil J.E., Hallett M., Siegel P.M.
Mol. Cancer Res. 5:1001-1014(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISEASE.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF322054 mRNA. Translation: AAK14240.1.
AJ251685 mRNA. Translation: CAB65272.1.
AK044764 mRNA. Translation: BAC32074.1.
AK076347 mRNA. Translation: BAC36306.1.
AK150739 mRNA. Translation: BAE29813.1.
AK154617 mRNA. Translation: BAE32716.1.
AK170506 mRNA. Translation: BAE41844.1.
AK171441 mRNA. Translation: BAE42454.1.
AK171465 mRNA. Translation: BAE42471.1.
AK171474 mRNA. Translation: BAE42479.1.
AK171517 mRNA. Translation: BAE42501.1.
BC026375 mRNA. Translation: AAH26375.1.
CCDSCCDS20122.1.
RefSeqNP_444340.3. NM_053110.4.
UniGeneMm.302602.

3D structure databases

ProteinModelPortalQ99P91.
SMRQ99P91. Positions 258-320.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ99P91. 1 interaction.

PTM databases

PhosphoSiteQ99P91.

Proteomic databases

MaxQBQ99P91.
PRIDEQ99P91.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031840; ENSMUSP00000031840; ENSMUSG00000029816.
GeneID93695.
KEGGmmu:93695.
UCSCuc012elo.1. mouse.

Organism-specific databases

CTD10457.
MGIMGI:1934765. Gpnmb.

Phylogenomic databases

eggNOGNOG40272.
GeneTreeENSGT00530000063573.
HOGENOMHOG000252941.
HOVERGENHBG051797.
InParanoidQ3U3R9.
OMAHTYVLNG.
OrthoDBEOG7GXPB9.
TreeFamTF334865.

Gene expression databases

ArrayExpressQ99P91.
BgeeQ99P91.
CleanExMM_GPNMB.
MM_NMB.
GenevestigatorQ99P91.

Family and domain databases

Gene3D2.60.40.670. 1 hit.
InterProIPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamPF00801. PKD. 1 hit.
[Graphical view]
SMARTSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMSSF49299. SSF49299. 1 hit.
PROSITEPS50093. PKD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGPNMB. mouse.
NextBio351449.
PROQ99P91.
SOURCESearch...

Entry information

Entry nameGPNMB_MOUSE
AccessionPrimary (citable) accession number: Q99P91
Secondary accession number(s): Q3U3R9 expand/collapse secondary AC list , Q8BVV9, Q8BXL4, Q9QXA0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot