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Protein

Ras-related protein Rab-9A

Gene

Rab9a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of proteins between the endosomes and the trans-Golgi network. Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22GTPBy similarity9
Nucleotide bindingi62 – 66GTPBy similarity5
Nucleotide bindingi124 – 127GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-6811440. Retrograde transport at the Trans-Golgi-Network.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-9A
Gene namesi
Name:Rab9a
Synonyms:Rab9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi619740. Rab9a.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor Curated; Cytoplasmic side Curated
  • Endoplasmic reticulum membrane By similarity
  • Golgi apparatus membrane By similarity
  • Late endosome 1 Publication
  • Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor Curated; Cytoplasmic side Curated
  • Cytoplasmic vesiclephagosome By similarity
  • Cytoplasmic vesicle membrane By similarity
  • Melanosome By similarity

  • Note: Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001211412 – 201Ras-related protein Rab-9AAdd BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei34PhosphoserineBy similarity1
Modified residuei179PhosphoserineBy similarity1
Modified residuei187PhosphothreonineBy similarity1
Lipidationi200S-geranylgeranyl cysteineBy similarity1
Lipidationi201S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ99P75.
PRIDEiQ99P75.

Expressioni

Gene expression databases

BgeeiENSRNOG00000030443.
GenevisibleiQ99P75. RN.

Interactioni

Subunit structurei

Interacts (preferentially in its GTP-bound form) with GCC2 (via its GRIP domain) (PubMed:16885419). Interacts (GTP-bound form) with SGSM1; the GDP-bound form has much lower affinity for SGSM1. Interacts with SGSM2. The GTP-bound form but not the GDP-bound form interacts with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) but does not interact with HPS1 alone (By similarity).By similarity1 Publication

Protein-protein interaction databases

MINTiMINT-4569971.
STRINGi10116.ENSRNOP00000050986.

Structurei

3D structure databases

ProteinModelPortaliQ99P75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi36 – 44Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ99P75.
KOiK07899.
OMAiKIDINER.
OrthoDBiEOG091G0JIV.
PhylomeDBiQ99P75.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99P75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKSSLFKI ILLGDGGVGK SSLMNRYVTN KFDSQLFHTI GVEFLNKDLE
60 70 80 90 100
VDGHFVTMQI WDTAGQERFR SLRTPFYRGS DCCLLTFSVD DSQSFQNLSN
110 120 130 140 150
WKKEFIYYAD VKEPESFPFV ILGNKTDIKE RQVSTEEAQA WCKDNGDYPY
160 170 180 190 200
FETSAKDSTN VAAAFEEAVR RILATEDRSD HLIQTDTVNL HRKPKPNSSC

C
Length:201
Mass (Da):22,896
Last modified:April 26, 2005 - v2
Checksum:i43F490EAA67CF7EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29T → A in AAG49586 (PubMed:12051767).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325692 mRNA. Translation: AAG49586.1.
BC070502 mRNA. Translation: AAH70502.1.
RefSeqiNP_445910.1. NM_053458.1.
UniGeneiRn.35289.

Genome annotation databases

EnsembliENSRNOT00000050018; ENSRNOP00000050986; ENSRNOG00000030443.
GeneIDi84589.
KEGGirno:84589.
UCSCiRGD:619740. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325692 mRNA. Translation: AAG49586.1.
BC070502 mRNA. Translation: AAH70502.1.
RefSeqiNP_445910.1. NM_053458.1.
UniGeneiRn.35289.

3D structure databases

ProteinModelPortaliQ99P75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4569971.
STRINGi10116.ENSRNOP00000050986.

Proteomic databases

PaxDbiQ99P75.
PRIDEiQ99P75.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000050018; ENSRNOP00000050986; ENSRNOG00000030443.
GeneIDi84589.
KEGGirno:84589.
UCSCiRGD:619740. rat.

Organism-specific databases

CTDi9367.
RGDi619740. Rab9a.

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ99P75.
KOiK07899.
OMAiKIDINER.
OrthoDBiEOG091G0JIV.
PhylomeDBiQ99P75.

Enzyme and pathway databases

ReactomeiR-RNO-6811440. Retrograde transport at the Trans-Golgi-Network.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

PROiQ99P75.

Gene expression databases

BgeeiENSRNOG00000030443.
GenevisibleiQ99P75. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB9A_RAT
AccessioniPrimary (citable) accession number: Q99P75
Secondary accession number(s): Q6NS34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: April 26, 2005
Last modified: November 30, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.