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Q99P74 (RB27B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-27B
Gene names
Name:Rab27b
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in targeting uroplakins to urothelial apical membranes By similarity.

Subunit structure

Interacts with SYTL2, SYTL4, MYRIP and MLPH. Interacts with RPH3A and RPH3A By similarity.

Subcellular location

Membrane; Lipid-anchor By similarity.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Cellular componentMembrane
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
Disulfide bond
Lipoprotein
Methylation
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmultivesicular body sorting pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of exocytosis

Inferred from electronic annotation. Source: Ensembl

protein transport

Inferred from electronic annotation. Source: InterPro

regulation of exocytosis

Inferred from direct assay PubMed 15451418. Source: RGD

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentGolgi stack

Inferred from electronic annotation. Source: Ensembl

apical plasma membrane

Inferred from direct assay PubMed 17067543. Source: RGD

multivesicular body membrane

Inferred from electronic annotation. Source: Ensembl

secretory granule membrane

Inferred from direct assay PubMed 17067543. Source: RGD

trans-Golgi network transport vesicle

Inferred from electronic annotation. Source: Ensembl

zymogen granule membrane

Inferred from direct assay PubMed 15451418. Source: RGD

   Molecular_functionGDP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 15039459PubMed 17067543. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 218217Ras-related protein Rab-27B
PRO_0000121226

Regions

Nucleotide binding16 – 249GTP By similarity
Nucleotide binding74 – 785GTP By similarity
Nucleotide binding133 – 1364GTP By similarity
Nucleotide binding163 – 1653GTP By similarity
Motif38 – 469Effector region By similarity

Amino acid modifications

Modified residue21N-acetylthreonine By similarity
Modified residue2181Cysteine methyl ester By similarity
Lipidation2161S-geranylgeranyl cysteine By similarity
Lipidation2181S-geranylgeranyl cysteine By similarity
Disulfide bond123 ↔ 188 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99P74 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 784C894F9B935FEA

FASTA21824,620
        10         20         30         40         50         60 
MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR VVYDTQGADG 

        70         80         90        100        110        120 
SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD LTSQQSFLNV RNWMSQLQAN 

       130        140        150        160        170        180 
AYCENPDIVL IGNKADLLDQ REVNERQARE LAEKYGIPYF ETSAATGQNV EKSVETLLDL 

       190        200        210 
IMKRMEKCVE KTQVPDTVNG VNSGKVDGEK PAEKKCAC 

« Hide

References

[1]"Expression of small GTP binding rab proteins in rat long bones."
Zhao H., Vaananen K.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Bone.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF325693 mRNA. Translation: AAG49587.1.
RefSeqNP_445911.1. NM_053459.1.
XP_006254992.1. XM_006254930.1.
UniGeneRn.112603.

3D structure databases

ProteinModelPortalQ99P74.
SMRQ99P74. Positions 6-190.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000016369.

Proteomic databases

PaxDbQ99P74.
PRIDEQ99P74.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016369; ENSRNOP00000016369; ENSRNOG00000012176.
GeneID84590.
KEGGrno:84590.

Organism-specific databases

CTD5874.
RGD620114. Rab27b.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00740000114962.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidQ99P74.
KOK07886.
OMAQNVEKSV.
OrthoDBEOG7FFMSC.
PhylomeDBQ99P74.
TreeFamTF312895.

Gene expression databases

GenevestigatorQ99P74.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio617189.
PROQ99P74.

Entry information

Entry nameRB27B_RAT
AccessionPrimary (citable) accession number: Q99P74
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families