Q99P74 (RB27B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-27B

Gene names

Name:

Rab27b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in targeting uroplakins to urothelial apical membranes By similarity.
Subunit structure	Interacts with SYTL2, SYTL4, MYRIP and MLPH. Interacts with RPH3A and RPH3A By similarity.
Subcellular location	Membrane; Lipid-anchor By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Disulfide bond Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multivesicular body sorting pathway Inferred from electronic annotation. Source: Compara positive regulation of exocytosis Inferred from electronic annotation. Source: Compara protein transport Inferred from electronic annotation. Source: InterPro regulation of exocytosis Inferred from direct assay PubMed 15451418. Source: RGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi stack Inferred from electronic annotation. Source: Compara apical plasma membrane Inferred from direct assay PubMed 17067543. Source: RGD multivesicular body membrane Inferred from electronic annotation. Source: Compara trans-Golgi network transport vesicle Inferred from electronic annotation. Source: Compara zymogen granule membrane Inferred from direct assay PubMed 15451418. Source: RGD
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 218	217	Ras-related protein Rab-27B		PRO_0000121226
Regions
	Nucleotide binding	16 – 24	9	GTP By similarity
	Nucleotide binding	74 – 78	5	GTP By similarity
	Nucleotide binding	133 – 136	4	GTP By similarity
	Nucleotide binding	163 – 165	3	GTP By similarity
	Motif	38 – 46	9	Effector region By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity
	Modified residue	218	1	Cysteine methyl ester By similarity
	Lipidation	216	1	S-geranylgeranyl cysteine By similarity
	Lipidation	218	1	S-geranylgeranyl cysteine By similarity
	Disulfide bond	123 ↔ 188		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99P74 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 784C894F9B935FEA	FASTA	218	24,620
	10 20 30 40 50 60 MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR VVYDTQGADG 70 80 90 100 110 120 SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD LTSQQSFLNV RNWMSQLQAN 130 140 150 160 170 180 AYCENPDIVL IGNKADLLDQ REVNERQARE LAEKYGIPYF ETSAATGQNV EKSVETLLDL 190 200 210 IMKRMEKCVE KTQVPDTVNG VNSGKVDGEK PAEKKCAC

References

[1]	"Expression of small GTP binding rab proteins in rat long bones." Zhao H., Vaananen K. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Bone.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF325693 mRNA. Translation: AAG49587.1.
IPI	IPI00200425.
RefSeq	NP_445911.1. NM_053459.1.
UniGene	Rn.112603.
3D structure databases
ProteinModelPortal	Q99P74.
SMR	Q99P74. Positions 6-190.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000016369.
Proteomic databases
PaxDb	Q99P74.
PRIDE	Q99P74.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016369; ENSRNOP00000016369; ENSRNOG00000012176.
GeneID	84590.
KEGG	rno:84590.
Organism-specific databases
CTD	5874.
RGD	620114. Rab27b.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00700000104288.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	Q99P74.
KO	K07886.
OMA	QNVEKSV.
OrthoDB	EOG4PRSRM.
Gene expression databases
Genevestigator	Q99P74.
GermOnline	ENSRNOG00000012176. Rattus norvegicus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617189.

Entry information

Entry name

RB27B_RAT

Accession

Primary (citable) accession number: Q99P74

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program