UniProtKB - Q99P69 (NUF2_MOUSE)
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Protein
Kinetochore protein Nuf2
Gene
Nuf2
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity
GO - Biological processi
- attachment of spindle microtubules to kinetochore Source: MGI
- cell division Source: UniProtKB-KW
- mitotic cell cycle Source: InterPro
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-141444. Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal. R-MMU-2467813. Separation of Sister Chromatids. R-MMU-2500257. Resolution of Sister Chromatid Cohesion. R-MMU-5663220. RHO GTPases Activate Formins. R-MMU-68877. Mitotic Prometaphase. |
Names & Taxonomyi
Protein namesi | Recommended name: Kinetochore protein Nuf2Alternative name(s): Cell division cycle-associated protein 1 |
Gene namesi | Name:Nuf2 Synonyms:Cdca1, Nuf2r |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914227. Nuf2. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249814 | 1 – 463 | Kinetochore protein Nuf2Add BLAST | 463 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 247 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Can be phosphorylated by AURKA and AURKB.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99P69. |
PaxDbi | Q99P69. |
PeptideAtlasi | Q99P69. |
PRIDEi | Q99P69. |
PTM databases
iPTMneti | Q99P69. |
PhosphoSitePlusi | Q99P69. |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026683. |
ExpressionAtlasi | Q99P69. baseline and differential. |
Genevisiblei | Q99P69. MM. |
Interactioni
Subunit structurei
Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B (By similarity).By similarity
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000028000. |
Structurei
3D structure databases
ProteinModelPortali | Q99P69. |
SMRi | Q99P69. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 385 | Interaction with the N-terminus of NDC80By similarityAdd BLAST | 385 | |
Regioni | 386 – 463 | Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexBy similarityAdd BLAST | 78 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 149 – 341 | Sequence analysisAdd BLAST | 193 | |
Coiled coili | 389 – 463 | Sequence analysisAdd BLAST | 75 |
Sequence similaritiesi
Belongs to the NUF2 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4438. Eukaryota. ENOG410ZI4C. LUCA. |
GeneTreei | ENSGT00390000004199. |
HOGENOMi | HOG000290701. |
HOVERGENi | HBG082069. |
InParanoidi | Q99P69. |
OMAi | REIVGCI. |
OrthoDBi | EOG091G0YDU. |
PhylomeDBi | Q99P69. |
TreeFami | TF101067. |
Family and domain databases
Gene3Di | 1.10.418.60. 1 hit. |
InterProi | View protein in InterPro IPR005549. Kinetochore_Nuf2. IPR038275. Nuf2_N_sf. |
Pfami | View protein in Pfam PF03800. Nuf2. 1 hit. |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q99P69-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
METLSFPRYN VAELVVHIRN KLLTGADGKN LSKSDFLPNP KSDVLYMIYM
60 70 80 90 100
KALQLVYGVR LEHFYMMPMN IEVTYPHLME GFLPVRSLFF YMDSFMPICR
110 120 130 140 150
VNDFEIVDIL NPRTNRTSRF LSGIINFIHF RETCLEKCEE FLLQNKSSMV
160 170 180 190 200
RMQQLSNVHQ EALMKLEKLN TVPAEEREEF KQFMDDIQEL QHLLNEEFRQ
210 220 230 240 250
KTTLLQEEYA KMKSDISEKT KHLNEQKLSL VSLKEVEDNL KSKIVDSPEK
260 270 280 290 300
LKNYKDKMKG TVQKLRSARE KVMEQYDIYR DSVDCLPSCQ LEVQLYQKKS
310 320 330 340 350
QDLADNREKL SSLLKESLNL EDQIESDSSE LKKLKTEENS LIRMTTVKKE
360 370 380 390 400
KLATARFKIN KKQEDVKHYK QAMIEDCNKV QEKRDAVCEQ VTTVNQEIHK
410 420 430 440 450
IKSAIQQLRD TKKREILKSQ EIFVNLKSAL EKYHEGIEKV AEERSAKLEE
460
KTAELKKRMV RMV
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | L → W in BAC41008 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 61 | L → R in BAC41008 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 101 | V → G in BAB27973 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 137 | K → E in AAK01427 (PubMed:11266451).Curated | 1 | |
Sequence conflicti | 144 | Q → P in BAC41008 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 157 | N → H in BAC41008 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 221 | K → Q in BAC41008 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020553 | 270 – 296 | EKVME…EVQLY → VSRFTLWHSLNYLVSVQISL LNFYAPL in isoform 2. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_020554 | 297 – 429 | Missing in isoform 2. 1 PublicationAdd BLAST | 133 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF326732 mRNA. Translation: AAK01427.1. AK012011 mRNA. Translation: BAB27973.1. AK089939 mRNA. Translation: BAC41008.1. Frameshift. AK132467 mRNA. Translation: BAE21183.1. AK162249 mRNA. Translation: BAE36815.1. BC020026 mRNA. Translation: AAH20026.1. |
CCDSi | CCDS15463.1. [Q99P69-1] |
RefSeqi | NP_075773.2. NM_023284.3. [Q99P69-1] XP_006497021.1. XM_006496958.1. |
UniGenei | Mm.151315. |
Genome annotation databases
Ensembli | ENSMUST00000028000; ENSMUSP00000028000; ENSMUSG00000026683. [Q99P69-1] ENSMUST00000111368; ENSMUSP00000106999; ENSMUSG00000026683. [Q99P69-1] |
GeneIDi | 66977. |
UCSCi | uc007dlj.1. mouse. [Q99P69-1] uc007dll.1. mouse. [Q99P69-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | NUF2_MOUSE | |
Accessioni | Q99P69Primary (citable) accession number: Q99P69 Secondary accession number(s): Q8BTK7, Q8VE05, Q9CST5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2006 |
Last sequence update: | September 19, 2006 | |
Last modified: | March 28, 2018 | |
This is version 108 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |