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Protein

Kinetochore protein Nuf2

Gene

Nuf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Nuf2
Alternative name(s):
Cell division cycle-associated protein 1
Gene namesi
Name:Nuf2
Synonyms:Cdca1, Nuf2r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914227 Nuf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498141 – 463Kinetochore protein Nuf2Add BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei247PhosphoserineCombined sources1

Post-translational modificationi

Can be phosphorylated by AURKA and AURKB.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99P69
PaxDbiQ99P69
PeptideAtlasiQ99P69
PRIDEiQ99P69

PTM databases

iPTMnetiQ99P69
PhosphoSitePlusiQ99P69

Expressioni

Gene expression databases

BgeeiENSMUSG00000026683
ExpressionAtlasiQ99P69 baseline and differential
GenevisibleiQ99P69 MM

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B (By similarity).By similarity

Protein-protein interaction databases

ComplexPortaliCPX-551 Ndc80 complex
STRINGi10090.ENSMUSP00000028000

Structurei

3D structure databases

ProteinModelPortaliQ99P69
SMRiQ99P69
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Interaction with the N-terminus of NDC80By similarityAdd BLAST385
Regioni386 – 463Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexBy similarityAdd BLAST78

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili149 – 341Sequence analysisAdd BLAST193
Coiled coili389 – 463Sequence analysisAdd BLAST75

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4438 Eukaryota
ENOG410ZI4C LUCA
GeneTreeiENSGT00390000004199
HOGENOMiHOG000290701
HOVERGENiHBG082069
InParanoidiQ99P69
KOiK11548
OMAiREIVGCI
OrthoDBiEOG091G0YDU
PhylomeDBiQ99P69
TreeFamiTF101067

Family and domain databases

Gene3Di1.10.418.60, 1 hit
InterProiView protein in InterPro
IPR005549 Kinetochore_Nuf2
IPR038275 Nuf2_N_sf
PfamiView protein in Pfam
PF03800 Nuf2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99P69-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METLSFPRYN VAELVVHIRN KLLTGADGKN LSKSDFLPNP KSDVLYMIYM
60 70 80 90 100
KALQLVYGVR LEHFYMMPMN IEVTYPHLME GFLPVRSLFF YMDSFMPICR
110 120 130 140 150
VNDFEIVDIL NPRTNRTSRF LSGIINFIHF RETCLEKCEE FLLQNKSSMV
160 170 180 190 200
RMQQLSNVHQ EALMKLEKLN TVPAEEREEF KQFMDDIQEL QHLLNEEFRQ
210 220 230 240 250
KTTLLQEEYA KMKSDISEKT KHLNEQKLSL VSLKEVEDNL KSKIVDSPEK
260 270 280 290 300
LKNYKDKMKG TVQKLRSARE KVMEQYDIYR DSVDCLPSCQ LEVQLYQKKS
310 320 330 340 350
QDLADNREKL SSLLKESLNL EDQIESDSSE LKKLKTEENS LIRMTTVKKE
360 370 380 390 400
KLATARFKIN KKQEDVKHYK QAMIEDCNKV QEKRDAVCEQ VTTVNQEIHK
410 420 430 440 450
IKSAIQQLRD TKKREILKSQ EIFVNLKSAL EKYHEGIEKV AEERSAKLEE
460
KTAELKKRMV RMV
Length:463
Mass (Da):54,594
Last modified:September 19, 2006 - v2
Checksum:i7330FFDB6E28AEAC
GO
Isoform 2 (identifier: Q99P69-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-296: EKVMEQYDIYRDSVDCLPSCQLEVQLY → VSRFTLWHSLNYLVSVQISLLNFYAPL
     297-429: Missing.

Note: No experimental confirmation available.
Show »
Length:330
Mass (Da):39,010
Checksum:iC9A4AE4C35703D4B
GO

Sequence cautioni

The sequence BAC41008 differs from that shown. Reason: Frameshift at position 63.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4L → W in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti61L → R in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti101V → G in BAB27973 (PubMed:16141072).Curated1
Sequence conflicti137K → E in AAK01427 (PubMed:11266451).Curated1
Sequence conflicti144Q → P in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti157N → H in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti221K → Q in BAC41008 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020553270 – 296EKVME…EVQLY → VSRFTLWHSLNYLVSVQISL LNFYAPL in isoform 2. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_020554297 – 429Missing in isoform 2. 1 PublicationAdd BLAST133

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326732 mRNA Translation: AAK01427.1
AK012011 mRNA Translation: BAB27973.1
AK089939 mRNA Translation: BAC41008.1 Frameshift.
AK132467 mRNA Translation: BAE21183.1
AK162249 mRNA Translation: BAE36815.1
BC020026 mRNA Translation: AAH20026.1
CCDSiCCDS15463.1 [Q99P69-1]
RefSeqiNP_075773.2, NM_023284.3 [Q99P69-1]
XP_006497021.1, XM_006496958.1
UniGeneiMm.151315

Genome annotation databases

EnsembliENSMUST00000028000; ENSMUSP00000028000; ENSMUSG00000026683 [Q99P69-1]
ENSMUST00000111368; ENSMUSP00000106999; ENSMUSG00000026683 [Q99P69-1]
GeneIDi66977
KEGGimmu:66977
UCSCiuc007dlj.1 mouse [Q99P69-1]
uc007dll.1 mouse [Q99P69-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUF2_MOUSE
AccessioniPrimary (citable) accession number: Q99P69
Secondary accession number(s): Q8BTK7, Q8VE05, Q9CST5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: June 20, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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