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UniProtKB - Q99P69 (NUF2_MOUSE)

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Protein

Kinetochore protein Nuf2

Gene

Nuf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity

GO - Biological processi

Complete GO annotation...

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Nuf2
Alternative name(s):
Cell division cycle-associated protein 1
Gene namesi
Name:Nuf2
Synonyms:Cdca1, Nuf2r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914227. Nuf2.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome › centromere › kinetochore By similarity

    • Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498141 – 463Kinetochore protein Nuf2Add BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei247PhosphoserineCombined sources1

Post-translational modificationi

Can be phosphorylated by AURKA and AURKB.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99P69.
PaxDbiQ99P69.
PeptideAtlasiQ99P69.
PRIDEiQ99P69.

PTM databases

iPTMnetiQ99P69.
PhosphoSitePlusiQ99P69.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026683.
ExpressionAtlasiQ99P69. baseline and differential.
GenevisibleiQ99P69. MM.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028000.

Structurei

3D structure databases

ProteinModelPortaliQ99P69.
SMRiQ99P69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Interaction with the N-terminus of NDC80By similarityAdd BLAST385
Regioni386 – 463Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexBy similarityAdd BLAST78

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili149 – 341Sequence analysisAdd BLAST193
Coiled coili389 – 463Sequence analysisAdd BLAST75

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4438. Eukaryota.
ENOG410ZI4C. LUCA.
GeneTreeiENSGT00390000004199.
HOGENOMiHOG000290701.
HOVERGENiHBG082069.
InParanoidiQ99P69.
KOiK11548.
OMAiREIVGCI.
OrthoDBiEOG091G0YDU.
PhylomeDBiQ99P69.
TreeFamiTF101067.

Family and domain databases

InterProiView protein in InterPro
IPR005549. Kinetochore_Nuf2.
PfamiView protein in Pfam
PF03800. Nuf2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99P69-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METLSFPRYN VAELVVHIRN KLLTGADGKN LSKSDFLPNP KSDVLYMIYM 
        60         70         80         90        100
KALQLVYGVR LEHFYMMPMN IEVTYPHLME GFLPVRSLFF YMDSFMPICR 
       110        120        130        140        150
VNDFEIVDIL NPRTNRTSRF LSGIINFIHF RETCLEKCEE FLLQNKSSMV 
       160        170        180        190        200
RMQQLSNVHQ EALMKLEKLN TVPAEEREEF KQFMDDIQEL QHLLNEEFRQ 
       210        220        230        240        250
KTTLLQEEYA KMKSDISEKT KHLNEQKLSL VSLKEVEDNL KSKIVDSPEK 
       260        270        280        290        300
LKNYKDKMKG TVQKLRSARE KVMEQYDIYR DSVDCLPSCQ LEVQLYQKKS 
       310        320        330        340        350
QDLADNREKL SSLLKESLNL EDQIESDSSE LKKLKTEENS LIRMTTVKKE 
       360        370        380        390        400
KLATARFKIN KKQEDVKHYK QAMIEDCNKV QEKRDAVCEQ VTTVNQEIHK 
       410        420        430        440        450
IKSAIQQLRD TKKREILKSQ EIFVNLKSAL EKYHEGIEKV AEERSAKLEE 
       460 
KTAELKKRMV RMV
Length:463
Mass (Da):54,594
Last modified:September 19, 2006 - v2
Checksum:i7330FFDB6E28AEAC
GO
Isoform 2 (identifier: Q99P69-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-296: EKVMEQYDIYRDSVDCLPSCQLEVQLY → VSRFTLWHSLNYLVSVQISLLNFYAPL
     297-429: Missing.

Note: No experimental confirmation available.
Show »
Length:330
Mass (Da):39,010
Checksum:iC9A4AE4C35703D4B
GO

Sequence cautioni

The sequence BAC41008 differs from that shown. Reason: Frameshift at position 63.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4L → W in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti61L → R in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti101V → G in BAB27973 (PubMed:16141072).Curated1
Sequence conflicti137K → E in AAK01427 (PubMed:11266451).Curated1
Sequence conflicti144Q → P in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti157N → H in BAC41008 (PubMed:16141072).Curated1
Sequence conflicti221K → Q in BAC41008 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020553270 – 296EKVME…EVQLY → VSRFTLWHSLNYLVSVQISL LNFYAPL in isoform 2. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_020554297 – 429Missing in isoform 2. 1 PublicationAdd BLAST133

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326732 mRNA. Translation: AAK01427.1.
AK012011 mRNA. Translation: BAB27973.1.
AK089939 mRNA. Translation: BAC41008.1. Frameshift.
AK132467 mRNA. Translation: BAE21183.1.
AK162249 mRNA. Translation: BAE36815.1.
BC020026 mRNA. Translation: AAH20026.1.
CCDSiCCDS15463.1. [Q99P69-1]
RefSeqiNP_075773.2. NM_023284.3. [Q99P69-1]
XP_006497021.1. XM_006496958.1. [Q99P69-1]
UniGeneiMm.151315.

Genome annotation databases

EnsembliENSMUST00000028000; ENSMUSP00000028000; ENSMUSG00000026683. [Q99P69-1]
ENSMUST00000111368; ENSMUSP00000106999; ENSMUSG00000026683. [Q99P69-1]
GeneIDi66977.
KEGGimmu:66977.
UCSCiuc007dlj.1. mouse. [Q99P69-1]
uc007dll.1. mouse. [Q99P69-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNUF2_MOUSE
AccessioniPrimary (citable) accession number: Q99P69
Secondary accession number(s): Q8BTK7, Q8VE05, Q9CST5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: July 5, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families