Nuf2

Functionⁱ

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity

GO - Biological processⁱ

attachment of spindle microtubules to kinetochore Source: MGI
cell division Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell cycle, Cell division, Mitosis

Biological process

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

Reactomeⁱ	R-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813 Separation of Sister Chromatids R-MMU-2500257 Resolution of Sister Chromatid Cohesion R-MMU-5663220 RHO GTPases Activate Formins R-MMU-68877 Mitotic Prometaphase

Reactomeⁱ

R-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Kinetochore protein Nuf2 Alternative name(s): Cell division cycle-associated protein 1
Gene namesⁱ	Name:Nuf2 Synonyms:Cdca1, Nuf2r
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:1914227 Nuf2

Keywords - Cellular componentⁱ

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000249814	1 – 463	Kinetochore protein Nuf2Add BLAST		463

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity		1
Modified residueⁱ	247	PhosphoserineCombined sources		1

Post-translational modificationⁱ

Can be phosphorylated by AURKA and AURKB.By similarity

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q99P69
PaxDbⁱ	Q99P69
PeptideAtlas More...PeptideAtlasⁱ	Q99P69
PRIDEⁱ	Q99P69

PTM databases

iPTMnetⁱ	Q99P69
PhosphoSitePlusⁱ	Q99P69

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000026683
ExpressionAtlasⁱ	Q99P69 baseline and differential
Genevisibleⁱ	Q99P69 MM

Interactionⁱ

Subunit structureⁱ

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B (By similarity).By similarity

Protein-protein interaction databases

ComplexPortalⁱ	CPX-551 Ndc80 complex
STRINGⁱ	10090.ENSMUSP00000028000

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q99P69
SMRⁱ	Q99P69
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 385	Interaction with the N-terminus of NDC80By similarityAdd BLAST		385
Regionⁱ	386 – 463	Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexBy similarityAdd BLAST		78

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	149 – 341	Sequence analysisAdd BLAST		193
Coiled coilⁱ	389 – 463	Sequence analysisAdd BLAST		75

Sequence similaritiesⁱ

Belongs to the NUF2 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	KOG4438 Eukaryota ENOG410ZI4C LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004199
HOGENOMⁱ	HOG000290701
HOVERGENⁱ	HBG082069
InParanoidⁱ	Q99P69
KEGG Orthology (KO) More...KOⁱ	K11548
OMAⁱ	REIVGCI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0YDU
PhylomeDBⁱ	Q99P69
TreeFamⁱ	TF101067

Family and domain databases

Gene3Dⁱ	1.10.418.60, 1 hit
InterProⁱ	View protein in InterPro IPR005549 Kinetochore_Nuf2 IPR038275 Nuf2_N_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03800 Nuf2, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: Q99P69-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METLSFPRYN VAELVVHIRN KLLTGADGKN LSKSDFLPNP KSDVLYMIYM 
        60         70         80         90        100
KALQLVYGVR LEHFYMMPMN IEVTYPHLME GFLPVRSLFF YMDSFMPICR 
       110        120        130        140        150
VNDFEIVDIL NPRTNRTSRF LSGIINFIHF RETCLEKCEE FLLQNKSSMV 
       160        170        180        190        200
RMQQLSNVHQ EALMKLEKLN TVPAEEREEF KQFMDDIQEL QHLLNEEFRQ 
       210        220        230        240        250
KTTLLQEEYA KMKSDISEKT KHLNEQKLSL VSLKEVEDNL KSKIVDSPEK 
       260        270        280        290        300
LKNYKDKMKG TVQKLRSARE KVMEQYDIYR DSVDCLPSCQ LEVQLYQKKS 
       310        320        330        340        350
QDLADNREKL SSLLKESLNL EDQIESDSSE LKKLKTEENS LIRMTTVKKE 
       360        370        380        390        400
KLATARFKIN KKQEDVKHYK QAMIEDCNKV QEKRDAVCEQ VTTVNQEIHK 
       410        420        430        440        450
IKSAIQQLRD TKKREILKSQ EIFVNLKSAL EKYHEGIEKV AEERSAKLEE 
       460 
KTAELKKRMV RMV

Length:463

Mass (Da):54,594

Last modified:September 19, 2006 - v2

Checksum:ⁱ7330FFDB6E28AEAC

GO

Isoform 2 (identifier: Q99P69-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
270-296: EKVMEQYDIYRDSVDCLPSCQLEVQLY → VSRFTLWHSLNYLVSVQISLLNFYAPL
297-429: Missing.

« Hide

        10         20         30         40         50
METLSFPRYN VAELVVHIRN KLLTGADGKN LSKSDFLPNP KSDVLYMIYM 
        60         70         80         90        100
KALQLVYGVR LEHFYMMPMN IEVTYPHLME GFLPVRSLFF YMDSFMPICR 
       110        120        130        140        150
VNDFEIVDIL NPRTNRTSRF LSGIINFIHF RETCLEKCEE FLLQNKSSMV 
       160        170        180        190        200
RMQQLSNVHQ EALMKLEKLN TVPAEEREEF KQFMDDIQEL QHLLNEEFRQ 
       210        220        230        240        250
KTTLLQEEYA KMKSDISEKT KHLNEQKLSL VSLKEVEDNL KSKIVDSPEK 
       260        270        280        290        300
LKNYKDKMKG TVQKLRSARV SRFTLWHSLN YLVSVQISLL NFYAPLLEKY 
       310        320        330
HEGIEKVAEE RSAKLEEKTA ELKKRMVRMV

Note: No experimental confirmation available.

Show »

Length:330

Mass (Da):39,010

Checksum:ⁱC9A4AE4C35703D4B

GO

Sequence cautionⁱ

The sequence BAC41008 differs from that shown. Reason: Frameshift at position 63.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	4	L → W in BAC41008 (PubMed:16141072).Curated	1
Sequence conflictⁱ	61	L → R in BAC41008 (PubMed:16141072).Curated	1
Sequence conflictⁱ	101	V → G in BAB27973 (PubMed:16141072).Curated	1
Sequence conflictⁱ	137	K → E in AAK01427 (PubMed:11266451).Curated	1
Sequence conflictⁱ	144	Q → P in BAC41008 (PubMed:16141072).Curated	1
Sequence conflictⁱ	157	N → H in BAC41008 (PubMed:16141072).Curated	1
Sequence conflictⁱ	221	K → Q in BAC41008 (PubMed:16141072).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_020553	270 – 296	EKVME…EVQLY → VSRFTLWHSLNYLVSVQISL LNFYAPL in isoform 2. 1 PublicationAdd BLAST		27
Alternative sequenceⁱVSP_020554	297 – 429	Missing in isoform 2. 1 PublicationAdd BLAST		133

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF326732 mRNA Translation: AAK01427.1 AK012011 mRNA Translation: BAB27973.1 AK089939 mRNA Translation: BAC41008.1 Frameshift. AK132467 mRNA Translation: BAE21183.1 AK162249 mRNA Translation: BAE36815.1 BC020026 mRNA Translation: AAH20026.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15463.1 [Q99P69-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_075773.2, NM_023284.3 [Q99P69-1] XP_006497021.1, XM_006496958.1
UniGeneⁱ	Mm.151315

Genome annotation databases

Ensemblⁱ	ENSMUST00000028000; ENSMUSP00000028000; ENSMUSG00000026683 [Q99P69-1] ENSMUST00000111368; ENSMUSP00000106999; ENSMUSG00000026683 [Q99P69-1]
GeneIDⁱ	66977
KEGGⁱ	mmu:66977
UCSC genome browser More...UCSCⁱ	uc007dlj.1 mouse [Q99P69-1] uc007dll.1 mouse [Q99P69-2]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q99P69	Kinetochore protein Nuf2		CHICK		469
Uncharacterized protein		CALSU		469
NUF2, NDC80 kinetochore complex component		MELGA		469
UPI000670A9A3		Anser cygnoides domesticus		423
Nuf2 (Fragment)		COTCO		278
+86
Q99P69-2	Kinetochore protein Nuf2		MOUSE		296
Kinetochore protein Nuf2		CHICK		281

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF326732 mRNA Translation: AAK01427.1 AK012011 mRNA Translation: BAB27973.1 AK089939 mRNA Translation: BAC41008.1 Frameshift. AK132467 mRNA Translation: BAE21183.1 AK162249 mRNA Translation: BAE36815.1 BC020026 mRNA Translation: AAH20026.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15463.1 [Q99P69-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_075773.2, NM_023284.3 [Q99P69-1] XP_006497021.1, XM_006496958.1
UniGeneⁱ	Mm.151315

3D structure databases

ProteinModelPortalⁱ	Q99P69
SMRⁱ	Q99P69
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-551 Ndc80 complex
STRINGⁱ	10090.ENSMUSP00000028000

PTM databases

iPTMnetⁱ	Q99P69
PhosphoSitePlusⁱ	Q99P69

Proteomic databases

EPDⁱ	Q99P69
PaxDbⁱ	Q99P69
PeptideAtlas More...PeptideAtlasⁱ	Q99P69
PRIDEⁱ	Q99P69

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000028000; ENSMUSP00000028000; ENSMUSG00000026683 [Q99P69-1] ENSMUST00000111368; ENSMUSP00000106999; ENSMUSG00000026683 [Q99P69-1]
GeneIDⁱ	66977
KEGGⁱ	mmu:66977
UCSC genome browser More...UCSCⁱ	uc007dlj.1 mouse [Q99P69-1] uc007dll.1 mouse [Q99P69-2]

Organism-specific databases

CTDⁱ	83540
MGIⁱ	MGI:1914227 Nuf2

Phylogenomic databases

eggNOGⁱ	KOG4438 Eukaryota ENOG410ZI4C LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004199
HOGENOMⁱ	HOG000290701
HOVERGENⁱ	HBG082069
InParanoidⁱ	Q99P69
KEGG Orthology (KO) More...KOⁱ	K11548
OMAⁱ	REIVGCI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0YDU
PhylomeDBⁱ	Q99P69
TreeFamⁱ	TF101067

Enzyme and pathway databases

Reactomeⁱ	R-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813 Separation of Sister Chromatids R-MMU-2500257 Resolution of Sister Chromatid Cohesion R-MMU-5663220 RHO GTPases Activate Formins R-MMU-68877 Mitotic Prometaphase

Reactomeⁱ

R-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q99P69
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000026683
ExpressionAtlasⁱ	Q99P69 baseline and differential
Genevisibleⁱ	Q99P69 MM

Family and domain databases

Gene3Dⁱ	1.10.418.60, 1 hit
InterProⁱ	View protein in InterPro IPR005549 Kinetochore_Nuf2 IPR038275 Nuf2_N_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03800 Nuf2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NUF2_MOUSE
Accessionⁱ	Q99P69Primary (citable) accession number: Q99P69 Secondary accession number(s): Q8BTK7, Q8VE05, Q9CST5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
	Last sequence update:	September 19, 2006
	Last modified:	June 20, 2018
	This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q99P69 (NUF2_MOUSE)

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Kinetochore protein Nuf2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ