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Protein

Ras-related protein Rab-27B

Gene

Rab27b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in targeting uroplakins to urothelial apical membranes.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 24GTP1 Publication9
Nucleotide bindingi74 – 78GTP1 Publication5
Nucleotide bindingi133 – 136GTP1 Publication4
Nucleotide bindingi163 – 165GTP1 Publication3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • myosin V binding Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-27B
Gene namesi
Name:Rab27b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1931295. Rab27b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6Y → F: Abolishes the interaction with MLPH. 1 Publication1
Mutagenesisi78Q → L: Loss of GTPase activity. 1 Publication1
Mutagenesisi91D → G: Strongly reduces the interaction with MLPH. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001212252 – 218Ras-related protein Rab-27BAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Disulfide bondi123 ↔ 1881 Publication
Lipidationi216S-geranylgeranyl cysteineBy similarity1
Modified residuei218Cysteine methyl esterBy similarity1
Lipidationi218S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ99P58.
MaxQBiQ99P58.
PaxDbiQ99P58.
PRIDEiQ99P58.

PTM databases

PhosphoSitePlusiQ99P58.

Expressioni

Tissue specificityi

Expressed abundantly in the stomach and is predominantly localized at the apical region of gastric-surface mucus cells. Also expressed in the brain and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000024511.
CleanExiMM_RAB27B.
ExpressionAtlasiQ99P58. baseline and differential.
GenevisibleiQ99P58. MM.

Interactioni

Subunit structurei

Interacts with SYTL2, SYTL4, MYRIP and MLPH. Interacts with RPH3A and RPH3A.5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219798. 2 interactors.
DIPiDIP-46237N.
IntActiQ99P58. 4 interactors.
STRINGi10090.ENSMUSP00000068349.

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 17Combined sources9
Helixi22 – 30Combined sources9
Beta strandi37 – 53Combined sources17
Beta strandi66 – 77Combined sources12
Helixi80 – 89Combined sources10
Beta strandi94 – 100Combined sources7
Helixi105 – 115Combined sources11
Turni122 – 124Combined sources3
Beta strandi127 – 133Combined sources7
Helixi138 – 140Combined sources3
Helixi146 – 153Combined sources8
Turni154 – 156Combined sources3
Beta strandi159 – 161Combined sources3
Turni164 – 167Combined sources4
Helixi170 – 187Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IEYX-ray3.18A/B1-193[»]
2IEZX-ray2.80A/B/H/I1-218[»]
2IF0X-ray2.80A/B1-198[»]
2ZETX-ray3.00A/B1-201[»]
ProteinModelPortaliQ99P58.
SMRiQ99P58.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99P58.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 46Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0081. Eukaryota.
ENOG410XQ3T. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ99P58.
KOiK07886.
OMAiAEKKCGC.
OrthoDBiEOG091G0I51.
PhylomeDBiQ99P58.
TreeFamiTF312895.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99P58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR
60 70 80 90 100
VVYDTQGADG ASGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD
110 120 130 140 150
LTSQQSFLNV RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE
160 170 180 190 200
LAEKYGIPYF ETSAATGQNV EKSVETLLDL IMKRMEKCVE KTQVPDTVNG
210
GNSGKLDGEK PAEKKCAC
Length:218
Mass (Da):24,560
Last modified:January 23, 2007 - v3
Checksum:i01DBC47275B4B955
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57G → R in AAG45502 (Ref. 2) Curated1
Sequence conflicti77G → E in AAG45502 (Ref. 2) Curated1
Sequence conflicti100D → V in AAG45502 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF328893 mRNA. Translation: AAK11242.1.
AY010898 mRNA. Translation: AAG45502.1.
BC032928 mRNA. Translation: AAH32928.1.
CCDSiCCDS29331.1.
RefSeqiNP_001076022.1. NM_001082553.2.
NP_001289727.1. NM_001302798.1.
NP_085031.3. NM_030554.4.
UniGeneiMm.246753.

Genome annotation databases

EnsembliENSMUST00000069749; ENSMUSP00000068349; ENSMUSG00000024511.
ENSMUST00000117692; ENSMUSP00000112807; ENSMUSG00000024511.
ENSMUST00000121693; ENSMUSP00000114094; ENSMUSG00000024511.
GeneIDi80718.
KEGGimmu:80718.
UCSCiuc008fnz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF328893 mRNA. Translation: AAK11242.1.
AY010898 mRNA. Translation: AAG45502.1.
BC032928 mRNA. Translation: AAH32928.1.
CCDSiCCDS29331.1.
RefSeqiNP_001076022.1. NM_001082553.2.
NP_001289727.1. NM_001302798.1.
NP_085031.3. NM_030554.4.
UniGeneiMm.246753.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IEYX-ray3.18A/B1-193[»]
2IEZX-ray2.80A/B/H/I1-218[»]
2IF0X-ray2.80A/B1-198[»]
2ZETX-ray3.00A/B1-201[»]
ProteinModelPortaliQ99P58.
SMRiQ99P58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219798. 2 interactors.
DIPiDIP-46237N.
IntActiQ99P58. 4 interactors.
STRINGi10090.ENSMUSP00000068349.

PTM databases

PhosphoSitePlusiQ99P58.

Proteomic databases

EPDiQ99P58.
MaxQBiQ99P58.
PaxDbiQ99P58.
PRIDEiQ99P58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069749; ENSMUSP00000068349; ENSMUSG00000024511.
ENSMUST00000117692; ENSMUSP00000112807; ENSMUSG00000024511.
ENSMUST00000121693; ENSMUSP00000114094; ENSMUSG00000024511.
GeneIDi80718.
KEGGimmu:80718.
UCSCiuc008fnz.2. mouse.

Organism-specific databases

CTDi5874.
MGIiMGI:1931295. Rab27b.

Phylogenomic databases

eggNOGiKOG0081. Eukaryota.
ENOG410XQ3T. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ99P58.
KOiK07886.
OMAiAEKKCGC.
OrthoDBiEOG091G0I51.
PhylomeDBiQ99P58.
TreeFamiTF312895.

Miscellaneous databases

ChiTaRSiRab27b. mouse.
EvolutionaryTraceiQ99P58.
PROiQ99P58.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024511.
CleanExiMM_RAB27B.
ExpressionAtlasiQ99P58. baseline and differential.
GenevisibleiQ99P58. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB27B_MOUSE
AccessioniPrimary (citable) accession number: Q99P58
Secondary accession number(s): Q8CIX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.