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Protein

Sphingosine-1-phosphate phosphatase 1

Gene

Sgpp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phoshate, lysophosphatidic acid or phosphatidic acid. Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (By similarity). Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide though a phosphorylation/dephosphorylation cycle. Regulates intracellular ceramide levels, which in turn regulate apoptosis (By similarity). Via S1P levels, modulates resting tone, intracellular Ca2+ and myogenic vasoconstriction in resistance arteries. Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei197Proton donorBy similarity1
Active sitei244NucleophileBy similarity1
Sitei248Stabilizes the active site histidine for nucleophilic attackBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine-1-phosphate phosphatase 1 (EC:3.1.3.-)
Short name:
SPPase1
Short name:
Spp1
Alternative name(s):
Sphingosine-1-phosphatase 1
Gene namesi
Name:Sgpp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727829 Sgpp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Transmembranei246 – 266HelicalSequence analysisAdd BLAST21
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001144791 – 430Sphingosine-1-phosphate phosphatase 1Add BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineCombined sources1
Modified residuei103PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99P55
PRIDEiQ99P55

PTM databases

iPTMnetiQ99P55
PhosphoSitePlusiQ99P55

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006913

Structurei

3D structure databases

ProteinModelPortaliQ99P55
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 175Phosphatase sequence motif ICurated9
Regioni194 – 197Phosphatase sequence motif IICurated4
Regioni237 – 248Phosphatase sequence motif IIICuratedAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQK8 Eukaryota
ENOG410Z1GR LUCA
HOGENOMiHOG000234228
HOVERGENiHBG079185
InParanoidiQ99P55
PhylomeDBiQ99P55

Family and domain databases

InterProiView protein in InterPro
IPR036938 P_Acid_Pase_2/haloperoxi_sf
IPR000326 P_Acid_Pase_2/haloperoxidase
PfamiView protein in Pfam
PF01569 PAP2, 1 hit
SMARTiView protein in SMART
SM00014 acidPPc, 1 hit
SUPFAMiSSF48317 SSF48317, 1 hit

Sequencei

Sequence statusi: Complete.

Q99P55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGQRLALL AIRLQEPQRV ASFQRLCGVE VPLGSPKAGE DAETEVRGAP
60 70 80 90 100
GDPRRRPRQS GADGSPAKPD CCGAPNGVRN GLAAEPGPTG PRRAGSLRRN
110 120 130 140 150
SLTGEEGELA KVSNLPLYYL FCFGTELGNE LFYIIFFPFW IWNLDPFVGR
160 170 180 190 200
RLVIIWVLVM YLGQCTKDII RWPRPASPPV IKLEIFYNSE YSMPSTHAMS
210 220 230 240 250
GTAIPIAMIL LTYGRWQYPL IYGLILIPCW SSLVCLSRIY MGMHSILDVI
260 270 280 290 300
AGFLYTILIL IIFYPLVDLI DNFNQTYKYA PLIIIGLHLI LGIFSFTLDT
310 320 330 340 350
WSTSRGDTAE ILGSGAGIAC GSHAAYNLGI SLDPSLHTLP LAIPPLTVTL
360 370 380 390 400
FGKAILRVVI GMLLVLFVRD IMKKVTIPLA CKLFGIPCHD LRQARQHMEV
410 420 430
ELPYRYITYG TVGFSITFLI PYIFSFIGIS
Length:430
Mass (Da):47,649
Last modified:October 10, 2003 - v2
Checksum:i0C95136FE9B14D4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03048654 Genomic DNA No translation available.
AABR03050704 Genomic DNA No translation available.
AF329638 mRNA Translation: AAK07473.1
UniGeneiRn.231866

Genome annotation databases

UCSCiRGD:727829 rat

Similar proteinsi

Entry informationi

Entry nameiSGPP1_RAT
AccessioniPrimary (citable) accession number: Q99P55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: February 28, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome