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Protein

Adenine DNA glycosylase

Gene

Mutyh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.2 Publications

Catalytic activityi

Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.1 Publication

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105Proton donor/acceptorBy similarity1
Sitei207Transition state stabilizerBy similarity1
Metal bindingi261Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi268Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi271Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi277Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: InterPro
  • DNA repair Source: MGI

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-110331 Cleavage of the damaged purine
R-MMU-110357 Displacement of DNA glycosylase by APEX1

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine DNA glycosylase (EC:3.2.2.311 Publication)
Alternative name(s):
MutY homolog
Short name:
mMYH
Gene namesi
Name:Mutyh
Synonyms:Myh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1917853 Mutyh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022401 – 515Adenine DNA glycosylaseAdd BLAST515

Proteomic databases

EPDiQ99P21
MaxQBiQ99P21
PaxDbiQ99P21
PRIDEiQ99P21

PTM databases

PhosphoSitePlusiQ99P21

Expressioni

Tissue specificityi

Expressed in heart, lung, liver, intestine, brain and thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000028687
CleanExiMM_MUTYH
ExpressionAtlasiQ99P21 baseline and differential
GenevisibleiQ99P21 MM

Interactioni

Protein-protein interaction databases

BioGridi214159, 3 interactors
STRINGi10090.ENSMUSP00000099760

Structurei

3D structure databases

ProteinModelPortaliQ99P21
SMRiQ99P21
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini335 – 466Nudix hydrolasePROSITE-ProRule annotationAdd BLAST132

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi376 – 398Nudix boxAdd BLAST23

Sequence similaritiesi

Belongs to the Nth/MutY family.Curated

Phylogenomic databases

eggNOGiKOG2457 Eukaryota
COG1194 LUCA
GeneTreeiENSGT00510000047220
HOGENOMiHOG000028743
HOVERGENiHBG052540
InParanoidiQ99P21
KOiK03575
OMAiGSKRSQV
OrthoDBiEOG091G04AB
TreeFamiTF328549

Family and domain databases

CDDicd03431 DNA_Glycosylase_C, 1 hit
cd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR029119 MutY_C
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
PF14815 NUDIX_4, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
SSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit
PS51462 NUDIX, 1 hit

Sequencei

Sequence statusi: Complete.

Q99P21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLQASVRS HKKQPANHKR RRTRALSSSQ AKPSSLDGLA KQKREELLQA
60 70 80 90 100
SVSPYHLFSD VADVTAFRSN LLSWYDQEKR DLPWRNLAKE EANSDRRAYA
110 120 130 140 150
VWVSEVMLQQ TQVATVIDYY TRWMQKWPKL QDLASASLEE VNQLWSGLGY
160 170 180 190 200
YSRGRRLQEG ARKVVEELGG HMPRTAETLQ QLLPGVGRYT AGAIASIAFD
210 220 230 240 250
QVTGVVDGNV LRVLCRVRAI GADPTSTLVS HHLWNLAQQL VDPARPGDFN
260 270 280 290 300
QAAMELGATV CTPQRPLCSH CPVQSLCRAY QRVQRGQLSA LPGRPDIEEC
310 320 330 340 350
ALNTRQCQLC LTSSSPWDPS MGVANFPRKA SRRPPREEYS ATCVVEQPGA
360 370 380 390 400
IGGPLVLLVQ RPDSGLLAGL WEFPSVTLEP SEQHQHKALL QELQRWCGPL
410 420 430 440 450
PAIRLQHLGE VIHIFSHIKL TYQVYSLALD QAPASTAPPG ARWLTWEEFC
460 470 480 490 500
NAAVSTAMKK VFRMYEDHRQ GTRKGSKRSQ VCPPSSRKKP SLGQQVLDTF
510
FQRHIPTDKP NSTTQ
Length:515
Mass (Da):57,723
Last modified:July 27, 2011 - v2
Checksum:i10DC39CC3A01AE5B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5Q → R in AAH57942 (PubMed:15489334).Curated1
Sequence conflicti284Q → E in AAG16632 (PubMed:11160897).Curated1
Sequence conflicti284Q → E in AAH57942 (PubMed:15489334).Curated1
Sequence conflicti312 – 313TS → PP in AAG16632 (PubMed:11160897).Curated2
Sequence conflicti312 – 313TS → PP in AAH57942 (PubMed:15489334).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007717 mRNA Translation: AAG16632.1
AB117938 mRNA Translation: BAC98380.1
AL683847 Genomic DNA No translation available.
BC057942 mRNA Translation: AAH57942.1
CCDSiCCDS18518.1
RefSeqiNP_001153053.1, NM_001159581.1
NP_573513.2, NM_133250.2
UniGeneiMm.180333

Genome annotation databases

EnsembliENSMUST00000102699; ENSMUSP00000099760; ENSMUSG00000028687
GeneIDi70603
KEGGimmu:70603
UCSCiuc008uhm.2 mouse

Entry informationi

Entry nameiMUTYH_MOUSE
AccessioniPrimary (citable) accession number: Q99P21
Secondary accession number(s): A2AGE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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