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Protein

Adenine DNA glycosylase

Gene

Mutyh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities (By similarity).By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051Proton donor/acceptorBy similarity
Sitei207 – 2071Transition state stabilizerBy similarity
Metal bindingi261 – 2611Iron-sulfur (4Fe-4S)By similarity
Metal bindingi268 – 2681Iron-sulfur (4Fe-4S)By similarity
Metal bindingi271 – 2711Iron-sulfur (4Fe-4S)By similarity
Metal bindingi277 – 2771Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: InterPro
  • DNA repair Source: MGI
  • response to oxidative stress Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-110331. Cleavage of the damaged purine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine DNA glycosylase (EC:3.2.2.-)
Alternative name(s):
MutY homolog
Short name:
mMYH
Gene namesi
Name:Mutyh
Synonyms:Myh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917853. Mutyh.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Adenine DNA glycosylasePRO_0000102240Add
BLAST

Proteomic databases

EPDiQ99P21.
PaxDbiQ99P21.
PRIDEiQ99P21.

PTM databases

PhosphoSiteiQ99P21.

Expressioni

Gene expression databases

BgeeiQ99P21.
CleanExiMM_MUTYH.
ExpressionAtlasiQ99P21. baseline and differential.
GenevisibleiQ99P21. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214159. 3 interactions.
STRINGi10090.ENSMUSP00000099760.

Structurei

3D structure databases

ProteinModelPortaliQ99P21.
SMRiQ99P21. Positions 53-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini335 – 466132Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi376 – 39823Nudix boxAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2457. Eukaryota.
COG1194. LUCA.
GeneTreeiENSGT00510000047220.
HOGENOMiHOG000028743.
HOVERGENiHBG052540.
InParanoidiQ99P21.
KOiK03575.
OMAiLCRARQR.
OrthoDBiEOG74XS6N.
TreeFamiTF328549.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR023170. HTH_base_excis_C.
IPR029119. MutY_C.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
PF14815. NUDIX_4. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
SSF55811. SSF55811. 1 hit.
PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
PS51462. NUDIX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99P21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLQASVRS HKKQPANHKR RRTRALSSSQ AKPSSLDGLA KQKREELLQA
60 70 80 90 100
SVSPYHLFSD VADVTAFRSN LLSWYDQEKR DLPWRNLAKE EANSDRRAYA
110 120 130 140 150
VWVSEVMLQQ TQVATVIDYY TRWMQKWPKL QDLASASLEE VNQLWSGLGY
160 170 180 190 200
YSRGRRLQEG ARKVVEELGG HMPRTAETLQ QLLPGVGRYT AGAIASIAFD
210 220 230 240 250
QVTGVVDGNV LRVLCRVRAI GADPTSTLVS HHLWNLAQQL VDPARPGDFN
260 270 280 290 300
QAAMELGATV CTPQRPLCSH CPVQSLCRAY QRVQRGQLSA LPGRPDIEEC
310 320 330 340 350
ALNTRQCQLC LTSSSPWDPS MGVANFPRKA SRRPPREEYS ATCVVEQPGA
360 370 380 390 400
IGGPLVLLVQ RPDSGLLAGL WEFPSVTLEP SEQHQHKALL QELQRWCGPL
410 420 430 440 450
PAIRLQHLGE VIHIFSHIKL TYQVYSLALD QAPASTAPPG ARWLTWEEFC
460 470 480 490 500
NAAVSTAMKK VFRMYEDHRQ GTRKGSKRSQ VCPPSSRKKP SLGQQVLDTF
510
FQRHIPTDKP NSTTQ
Length:515
Mass (Da):57,723
Last modified:July 27, 2011 - v2
Checksum:i10DC39CC3A01AE5B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51Q → R in AAH57942 (PubMed:15489334).Curated
Sequence conflicti284 – 2841Q → E in AAG16632 (PubMed:11160897).Curated
Sequence conflicti284 – 2841Q → E in AAH57942 (PubMed:15489334).Curated
Sequence conflicti312 – 3132TS → PP in AAG16632 (PubMed:11160897).Curated
Sequence conflicti312 – 3132TS → PP in AAH57942 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007717 mRNA. Translation: AAG16632.1.
AB117938 mRNA. Translation: BAC98380.1.
AL683847 Genomic DNA. Translation: CAM13543.1.
BC057942 mRNA. Translation: AAH57942.1.
CCDSiCCDS18518.1.
RefSeqiNP_001153053.1. NM_001159581.1.
NP_573513.2. NM_133250.2.
UniGeneiMm.180333.

Genome annotation databases

EnsembliENSMUST00000102699; ENSMUSP00000099760; ENSMUSG00000028687.
GeneIDi70603.
KEGGimmu:70603.
UCSCiuc008uhm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007717 mRNA. Translation: AAG16632.1.
AB117938 mRNA. Translation: BAC98380.1.
AL683847 Genomic DNA. Translation: CAM13543.1.
BC057942 mRNA. Translation: AAH57942.1.
CCDSiCCDS18518.1.
RefSeqiNP_001153053.1. NM_001159581.1.
NP_573513.2. NM_133250.2.
UniGeneiMm.180333.

3D structure databases

ProteinModelPortaliQ99P21.
SMRiQ99P21. Positions 53-467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214159. 3 interactions.
STRINGi10090.ENSMUSP00000099760.

PTM databases

PhosphoSiteiQ99P21.

Proteomic databases

EPDiQ99P21.
PaxDbiQ99P21.
PRIDEiQ99P21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102699; ENSMUSP00000099760; ENSMUSG00000028687.
GeneIDi70603.
KEGGimmu:70603.
UCSCiuc008uhm.2. mouse.

Organism-specific databases

CTDi4595.
MGIiMGI:1917853. Mutyh.

Phylogenomic databases

eggNOGiKOG2457. Eukaryota.
COG1194. LUCA.
GeneTreeiENSGT00510000047220.
HOGENOMiHOG000028743.
HOVERGENiHBG052540.
InParanoidiQ99P21.
KOiK03575.
OMAiLCRARQR.
OrthoDBiEOG74XS6N.
TreeFamiTF328549.

Enzyme and pathway databases

ReactomeiR-MMU-110331. Cleavage of the damaged purine.
R-MMU-110357. Displacement of DNA glycosylase by APEX1.

Miscellaneous databases

PROiQ99P21.
SOURCEiSearch...

Gene expression databases

BgeeiQ99P21.
CleanExiMM_MUTYH.
ExpressionAtlasiQ99P21. baseline and differential.
GenevisibleiQ99P21. MM.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR023170. HTH_base_excis_C.
IPR029119. MutY_C.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
PF14815. NUDIX_4. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
SSF55811. SSF55811. 1 hit.
PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
PS51462. NUDIX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Enhanced activity of adenine-DNA glycosylase (Myh) by apurinic/apyrimidinic endonuclease (Ape1) in mammalian base excision repair of an A/GO mismatch."
    Yang H., Clendenin W.M., Wong D., Demple B., Slupska M.M., Chiang J.-H., Miller J.H.
    Nucleic Acids Res. 29:743-752(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification and characterization of two forms of mouse MUTYH proteins encoded by alternatively spliced transcripts."
    Ichinoe A., Behmanesh M., Tominaga Y., Ushijima Y., Hirano S., Sakai Y., Tsuchimoto D., Sakumi K., Wake N., Nakabeppu Y.
    Nucleic Acids Res. 32:477-487(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiMUTYH_MOUSE
AccessioniPrimary (citable) accession number: Q99P21
Secondary accession number(s): A2AGE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.