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Q99NH8

- TREM2_MOUSE

UniProt

Q99NH8 - TREM2_MOUSE

Protein

Triggering receptor expressed on myeloid cells 2

Gene

Trem2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.1 Publication

    GO - Molecular functioni

    1. lipopolysaccharide binding Source: BHF-UCL
    2. lipoteichoic acid binding Source: BHF-UCL
    3. peptidoglycan binding Source: BHF-UCL
    4. protein binding Source: MGI
    5. transmembrane signaling receptor activity Source: MGI

    GO - Biological processi

    1. cellular response to lipoteichoic acid Source: BHF-UCL
    2. cellular response to peptidoglycan Source: BHF-UCL
    3. detection of lipopolysaccharide Source: BHF-UCL
    4. detection of lipoteichoic acid Source: BHF-UCL
    5. detection of peptidoglycan Source: BHF-UCL
    6. innate immune response Source: BHF-UCL
    7. lipopolysaccharide-mediated signaling pathway Source: BHF-UCL

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    ReactomeiREACT_188185. DAP12 signaling.
    REACT_188188. DAP12 interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Triggering receptor expressed on myeloid cells 2
    Short name:
    TREM-2
    Alternative name(s):
    Triggering receptor expressed on monocytes 2
    Gene namesi
    Name:Trem2
    Synonyms:Trem2a, Trem2b, Trem2c
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1913150. Trem2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 227209Triggering receptor expressed on myeloid cells 2PRO_0000014988Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi36 ↔ 110Sequence Analysis
    Disulfide bondi51 ↔ 60Sequence Analysis
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ99NH8.
    PRIDEiQ99NH8.

    PTM databases

    PhosphoSiteiQ99NH8.

    Expressioni

    Tissue specificityi

    Expressed at higher levels in the CNS, heart and lung than in lymph nodes or in other non-lymphoid tissues such as kidney, liver and testis. In the CNS not all microglia express TREM2. Brain regions with an incomplete blood-brain barrier had the lowest percentages of TREM2 expressing microglia, whereas the lateral entorhinal and cingulate cortex had the highest percentages.2 Publications

    Gene expression databases

    BgeeiQ99NH8.
    CleanExiMM_TREM2.
    GenevestigatoriQ99NH8.

    Interactioni

    Subunit structurei

    Interacts with TYROBP/DAP12.1 Publication

    Protein-protein interaction databases

    DIPiDIP-59896N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99NH8.
    SMRiQ99NH8. Positions 26-110.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 171153ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini193 – 22735CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 11284Ig-like V-typeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42611.
    GeneTreeiENSGT00470000042297.
    HOGENOMiHOG000118075.
    HOVERGENiHBG058623.
    KOiK14378.
    OMAiQVEHSTS.
    OrthoDBiEOG7B8S4S.
    PhylomeDBiQ99NH8.
    TreeFamiTF334441.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view]
    PfamiPF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99NH8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGPLHQFLLL LITALSQALN TTVLQGMAGQ SLRVSCTYDA LKHWGRRKAW    50
    CRQLGEEGPC QRVVSTHGVW LLAFLKKRNG STVIADDTLA GTVTITLKNL 100
    QAGDAGLYQC QSLRGREAEV LQKVLVEVLE DPLDDQDAGD LWVPEESSSF 150
    EGAQVEHSTS RNQETSFPPT SILLLLACVL LSKFLAASIL WAVARGRQKP 200
    GTPVVRGLDC GQDAGHQLQI LTGPGGT 227
    Length:227
    Mass (Da):24,527
    Last modified:June 1, 2001 - v1
    Checksum:i740EEA8D90BF9773
    GO
    Isoform 2 (identifier: Q99NH8-2) [UniParc]FASTAAdd to Basket

    Also known as: svTREM-2b

    The sequence of this isoform differs from the canonical sequence as follows:
         162-227: NQETSFPPTS...LQILTGPGGT → QVSSCGSPLA...HWTRRYVREF

    Show »
    Length:249
    Mass (Da):27,304
    Checksum:i99D0BC9909FDA704
    GO

    Sequence cautioni

    The sequence AAF69825.1 differs from that shown. Reason: Frameshift at positions 70 and 78.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 1051A → S in AAK01465. (PubMed:11241283)Curated
    Sequence conflicti105 – 1051A → S in AAH32959. (PubMed:15489334)Curated
    Sequence conflicti118 – 1181A → R in AAF69825. (PubMed:10799849)Curated
    Sequence conflicti122 – 1221Q → K in AAK01465. (PubMed:11241283)Curated
    Sequence conflicti122 – 1221Q → K in AAH32959. (PubMed:15489334)Curated
    Sequence conflicti148 – 1481S → E in AAK01465. (PubMed:11241283)Curated
    Sequence conflicti148 – 1481S → E in AAH32959. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti78 – 781R → W in strain: FVB/N. 1 Publication

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei162 – 22766NQETS…GPGGT → QVSSCGSPLAYHLPPLSKES RDLLPTHLHSSPPGLRSPEQ VSCSQHPLGCGQGQAEAGNT CGQRAGLWPRCWAPTSDPHW TRRYVREF in isoform 2. 1 PublicationVSP_010794Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF213458 mRNA. Translation: AAF69825.1. Frameshift.
    AY024348 mRNA. Translation: AAK01465.1.
    AY024349 mRNA. Translation: AAK01466.1.
    AY187009 mRNA. Translation: AAO06114.1.
    AK039477 mRNA. Translation: BAC30362.1.
    BC032959 mRNA. Translation: AAH32959.1.
    BC033485 mRNA. Translation: AAH33485.1.
    BC052784 mRNA. Translation: AAH52784.1.
    CCDSiCCDS28865.1. [Q99NH8-1]
    CCDS70825.1. [Q99NH8-2]
    RefSeqiNP_001259007.1. NM_001272078.1. [Q99NH8-2]
    NP_112544.1. NM_031254.3. [Q99NH8-1]
    UniGeneiMm.261623.

    Genome annotation databases

    EnsembliENSMUST00000024791; ENSMUSP00000024791; ENSMUSG00000023992. [Q99NH8-1]
    ENSMUST00000113237; ENSMUSP00000108863; ENSMUSG00000023992. [Q99NH8-2]
    GeneIDi83433.
    KEGGimmu:83433.
    UCSCiuc008cxj.1. mouse. [Q99NH8-1]
    uc008cxk.1. mouse. [Q99NH8-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF213458 mRNA. Translation: AAF69825.1 . Frameshift.
    AY024348 mRNA. Translation: AAK01465.1 .
    AY024349 mRNA. Translation: AAK01466.1 .
    AY187009 mRNA. Translation: AAO06114.1 .
    AK039477 mRNA. Translation: BAC30362.1 .
    BC032959 mRNA. Translation: AAH32959.1 .
    BC033485 mRNA. Translation: AAH33485.1 .
    BC052784 mRNA. Translation: AAH52784.1 .
    CCDSi CCDS28865.1. [Q99NH8-1 ]
    CCDS70825.1. [Q99NH8-2 ]
    RefSeqi NP_001259007.1. NM_001272078.1. [Q99NH8-2 ]
    NP_112544.1. NM_031254.3. [Q99NH8-1 ]
    UniGenei Mm.261623.

    3D structure databases

    ProteinModelPortali Q99NH8.
    SMRi Q99NH8. Positions 26-110.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59896N.

    PTM databases

    PhosphoSitei Q99NH8.

    Proteomic databases

    PaxDbi Q99NH8.
    PRIDEi Q99NH8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024791 ; ENSMUSP00000024791 ; ENSMUSG00000023992 . [Q99NH8-1 ]
    ENSMUST00000113237 ; ENSMUSP00000108863 ; ENSMUSG00000023992 . [Q99NH8-2 ]
    GeneIDi 83433.
    KEGGi mmu:83433.
    UCSCi uc008cxj.1. mouse. [Q99NH8-1 ]
    uc008cxk.1. mouse. [Q99NH8-2 ]

    Organism-specific databases

    CTDi 54209.
    MGIi MGI:1913150. Trem2.

    Phylogenomic databases

    eggNOGi NOG42611.
    GeneTreei ENSGT00470000042297.
    HOGENOMi HOG000118075.
    HOVERGENi HBG058623.
    KOi K14378.
    OMAi QVEHSTS.
    OrthoDBi EOG7B8S4S.
    PhylomeDBi Q99NH8.
    TreeFami TF334441.

    Enzyme and pathway databases

    Reactomei REACT_188185. DAP12 signaling.
    REACT_188188. DAP12 interactions.

    Miscellaneous databases

    NextBioi 350558.
    PROi Q99NH8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q99NH8.
    CleanExi MM_TREM2.
    Genevestigatori Q99NH8.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view ]
    Pfami PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes."
      Bouchon A., Dietrich J., Colonna M.
      J. Immunol. 164:4991-4995(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Cloning and characterization of a novel mouse myeloid DAP12-associated receptor family."
      Daws M.R., Lanier L.L., Seaman W.E., Ryan J.C.
      Eur. J. Immunol. 31:783-791(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH TYROBP/DAP12.
      Strain: BALB/c.
      Tissue: Macrophage.
    3. "Heterogeneous expression of the triggering receptor expressed on myeloid cells-2 on adult murine microglia."
      Schmid C.D., Sautkulis L.N., Danielson P.E., Cooper J., Hasel K.W., Hilbush B.S., Sutcliffe J.G., Carson M.J.
      J. Neurochem. 83:1309-1320(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
      Strain: C57BL/6J.
      Tissue: Brain.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Spinal cord.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT TRP-78.
      Strain: Czech II and FVB/N.
      Tissue: Lung and Mammary gland.

    Entry informationi

    Entry nameiTREM2_MOUSE
    AccessioniPrimary (citable) accession number: Q99NH8
    Secondary accession number(s): Q8CGK4
    , Q8CIA6, Q99NH9, Q9JL34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3