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Q99NH8 (TREM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Triggering receptor expressed on myeloid cells 2

Short name=TREM-2
Alternative name(s):
Triggering receptor expressed on monocytes 2
Gene names
Name:Trem2
Synonyms:Trem2a, Trem2b, Trem2c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Ref.2

Subunit structure

Interacts with TYROBP/DAP12. Ref.2

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein Potential.

Isoform 2: Secreted Potential.

Tissue specificity

Expressed at higher levels in the CNS, heart and lung than in lymph nodes or in other non-lymphoid tissues such as kidney, liver and testis. In the CNS not all microglia express TREM2. Brain regions with an incomplete blood-brain barrier had the lowest percentages of TREM2 expressing microglia, whereas the lateral entorhinal and cingulate cortex had the highest percentages. Ref.2 Ref.3

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution

The sequence AAF69825.1 differs from that shown. Reason: Frameshift at positions 70 and 78.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99NH8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99NH8-2)

Also known as: svTREM-2b;

The sequence of this isoform differs from the canonical sequence as follows:
     162-227: NQETSFPPTS...LQILTGPGGT → QVSSCGSPLA...HWTRRYVREF

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 227209Triggering receptor expressed on myeloid cells 2
PRO_0000014988

Regions

Topological domain19 – 171153Extracellular Potential
Transmembrane172 – 19221Helical; Potential
Topological domain193 – 22735Cytoplasmic Potential
Domain29 – 11284Ig-like V-type

Amino acid modifications

Glycosylation201N-linked (GlcNAc...) Potential
Glycosylation791N-linked (GlcNAc...) Potential
Disulfide bond36 ↔ 110 Potential
Disulfide bond51 ↔ 60 Potential

Natural variations

Alternative sequence162 – 22766NQETS…GPGGT → QVSSCGSPLAYHLPPLSKES RDLLPTHLHSSPPGLRSPEQ VSCSQHPLGCGQGQAEAGNT CGQRAGLWPRCWAPTSDPHW TRRYVREF in isoform 2.
VSP_010794
Natural variant781R → W in strain: FVB/N. Ref.5

Experimental info

Sequence conflict1051A → S in AAK01465. Ref.2
Sequence conflict1051A → S in AAH32959. Ref.5
Sequence conflict1181A → R in AAF69825. Ref.1
Sequence conflict1221Q → K in AAK01465. Ref.2
Sequence conflict1221Q → K in AAH32959. Ref.5
Sequence conflict1481S → E in AAK01465. Ref.2
Sequence conflict1481S → E in AAH32959. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 740EEA8D90BF9773

FASTA22724,527
        10         20         30         40         50         60 
MGPLHQFLLL LITALSQALN TTVLQGMAGQ SLRVSCTYDA LKHWGRRKAW CRQLGEEGPC 

        70         80         90        100        110        120 
QRVVSTHGVW LLAFLKKRNG STVIADDTLA GTVTITLKNL QAGDAGLYQC QSLRGREAEV 

       130        140        150        160        170        180 
LQKVLVEVLE DPLDDQDAGD LWVPEESSSF EGAQVEHSTS RNQETSFPPT SILLLLACVL 

       190        200        210        220 
LSKFLAASIL WAVARGRQKP GTPVVRGLDC GQDAGHQLQI LTGPGGT 

« Hide

Isoform 2 (svTREM-2b) [UniParc].

Checksum: 99D0BC9909FDA704
Show »

FASTA24927,304

References

« Hide 'large scale' references
[1]"Inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes."
Bouchon A., Dietrich J., Colonna M.
J. Immunol. 164:4991-4995(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning and characterization of a novel mouse myeloid DAP12-associated receptor family."
Daws M.R., Lanier L.L., Seaman W.E., Ryan J.C.
Eur. J. Immunol. 31:783-791(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH TYROBP/DAP12.
Strain: BALB/c.
Tissue: Macrophage.
[3]"Heterogeneous expression of the triggering receptor expressed on myeloid cells-2 on adult murine microglia."
Schmid C.D., Sautkulis L.N., Danielson P.E., Cooper J., Hasel K.W., Hilbush B.S., Sutcliffe J.G., Carson M.J.
J. Neurochem. 83:1309-1320(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Strain: C57BL/6J.
Tissue: Brain.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Spinal cord.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT TRP-78.
Strain: Czech II and FVB/N.
Tissue: Lung and Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF213458 mRNA. Translation: AAF69825.1. Frameshift.
AY024348 mRNA. Translation: AAK01465.1.
AY024349 mRNA. Translation: AAK01466.1.
AY187009 mRNA. Translation: AAO06114.1.
AK039477 mRNA. Translation: BAC30362.1.
BC032959 mRNA. Translation: AAH32959.1.
BC033485 mRNA. Translation: AAH33485.1.
BC052784 mRNA. Translation: AAH52784.1.
RefSeqNP_001259007.1. NM_001272078.1.
NP_112544.1. NM_031254.3.
UniGeneMm.261623.

3D structure databases

ProteinModelPortalQ99NH8.
SMRQ99NH8. Positions 26-145.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59896N.

PTM databases

PhosphoSiteQ99NH8.

Proteomic databases

PaxDbQ99NH8.
PRIDEQ99NH8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000024791; ENSMUSP00000024791; ENSMUSG00000023992. [Q99NH8-1]
ENSMUST00000113237; ENSMUSP00000108863; ENSMUSG00000023992. [Q99NH8-2]
GeneID83433.
KEGGmmu:83433.
UCSCuc008cxj.1. mouse. [Q99NH8-1]
uc008cxk.1. mouse. [Q99NH8-2]

Organism-specific databases

CTD54209.
MGIMGI:1913150. Trem2.

Phylogenomic databases

eggNOGNOG42611.
GeneTreeENSGT00470000042297.
HOGENOMHOG000118075.
HOVERGENHBG058623.
KOK14378.
OMAQVEHSTS.
OrthoDBEOG7B8S4S.
PhylomeDBQ99NH8.
TreeFamTF334441.

Gene expression databases

BgeeQ99NH8.
CleanExMM_TREM2.
GenevestigatorQ99NH8.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio350558.
PROQ99NH8.
SOURCESearch...

Entry information

Entry nameTREM2_MOUSE
AccessionPrimary (citable) accession number: Q99NH8
Secondary accession number(s): Q8CGK4 expand/collapse secondary AC list , Q8CIA6, Q99NH9, Q9JL34
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot