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Protein

Prestin

Gene

Slc26a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell shape, Hearing

Protein family/group databases

TCDBi2.A.53.2.5. the sulfate permease (sulp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene namesi
Name:Slc26a5
Synonyms:Pres
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1933154. Slc26a5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 79CytoplasmicSequence analysisAdd BLAST79
Transmembranei80 – 100Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini101 – 102ExtracellularSequence analysis2
Transmembranei103 – 123Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini124 – 131CytoplasmicSequence analysis8
Transmembranei132 – 152Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini153 – 184ExtracellularSequence analysisAdd BLAST32
Transmembranei185 – 205Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini206 – 211CytoplasmicSequence analysis6
Transmembranei212 – 232Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini233 – 253ExtracellularSequence analysisAdd BLAST21
Transmembranei254 – 274Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini275 – 286CytoplasmicSequence analysisAdd BLAST12
Transmembranei287 – 307Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini308 – 334ExtracellularSequence analysisAdd BLAST27
Transmembranei335 – 355Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini356 – 374CytoplasmicSequence analysisAdd BLAST19
Transmembranei375 – 395Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini396 – 411ExtracellularSequence analysisAdd BLAST16
Transmembranei412 – 432Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini433 – 441CytoplasmicSequence analysis9
Transmembranei442 – 462Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini463 – 479ExtracellularSequence analysisAdd BLAST17
Transmembranei480 – 500Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini501 – 744CytoplasmicSequence analysisAdd BLAST244

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • cytoplasm Source: Ensembl
  • integral component of plasma membrane Source: GO_Central
  • lateral plasma membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000801691 – 744PrestinAdd BLAST744

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ99NH7.
PRIDEiQ99NH7.

PTM databases

iPTMnetiQ99NH7.
PhosphoSitePlusiQ99NH7.

Expressioni

Tissue specificityi

Expressed in the outer hair cells (OHC) of the organ of Corti of the inner ear. Also weak expression in brain and testis. Very weakly expressed in heart, spleen, muscle and lactating mammary glands.1 Publication

Gene expression databases

BgeeiENSMUSG00000029015.
CleanExiMM_SLC26A5.
ExpressionAtlasiQ99NH7. baseline and differential.
GenevisibleiQ99NH7. MM.

Interactioni

GO - Molecular functioni

  • spectrin binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030878.

Structurei

3D structure databases

ProteinModelPortaliQ99NH7.
SMRiQ99NH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini525 – 713STASPROSITE-ProRule annotationAdd BLAST189

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0236. Eukaryota.
COG0659. LUCA.
GeneTreeiENSGT00760000119026.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ99NH7.
KOiK14703.
OMAiVGNANMA.
OrthoDBiEOG091G07RT.
TreeFamiTF313784.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR030282. Prestin.
IPR018045. S04_transporter_CS.
IPR011547. SLC26A/SulP_dom.
IPR001902. SLC26A/SulP_fam.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 2 hits.
PTHR11814:SF32. PTHR11814:SF32. 2 hits.
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99NH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHAEENEIP AETQRYYVER PIFSHPVLQE RLHVKDKVTE SIGDKLKQAF
60 70 80 90 100
TCTPKKIRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA
110 120 130 140 150
FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR
160 170 180 190 200
LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF
210 220 230 240 250
VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV
260 270 280 290 300
LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG
310 320 330 340 350
TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG
360 370 380 390 400
FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS
410 420 430 440 450
LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG
460 470 480 490 500
MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI
510 520 530 540 550
YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD
560 570 580 590 600
LYSSALKRKT GVNPALIMGA RRKAMRKYAK EVGNANVANA TVVKVDAEVD
610 620 630 640 650
GENATKPEEE DDEVKFPPIV IKTTFPEELQ RFLPQGENVH TVILDFTQVN
660 670 680 690 700
FVDSVGVKTL AGIVKEYGDV GIYVYLAGCS PQVVNDLTRN NFFENPALKE
710 720 730 740
LLFHSIHDAV LGSQVREAMA EQEATASLPQ EDMEPNATPT TPEA
Length:744
Mass (Da):81,337
Last modified:July 27, 2011 - v3
Checksum:i37A2524F5E75E31B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti469L → P in AAG59999 (PubMed:14553901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024359 mRNA. Translation: AAG59999.2.
AF529192 mRNA. Translation: AAO59381.1.
CH466586 Genomic DNA. Translation: EDL03187.1.
CCDSiCCDS19109.1.
RefSeqiNP_001276716.1. NM_001289787.1.
NP_001276717.1. NM_001289788.1.
NP_109652.3. NM_030727.5.
UniGeneiMm.160370.

Genome annotation databases

EnsembliENSMUST00000030878; ENSMUSP00000030878; ENSMUSG00000029015.
GeneIDi80979.
KEGGimmu:80979.
UCSCiuc008wpe.2. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024359 mRNA. Translation: AAG59999.2.
AF529192 mRNA. Translation: AAO59381.1.
CH466586 Genomic DNA. Translation: EDL03187.1.
CCDSiCCDS19109.1.
RefSeqiNP_001276716.1. NM_001289787.1.
NP_001276717.1. NM_001289788.1.
NP_109652.3. NM_030727.5.
UniGeneiMm.160370.

3D structure databases

ProteinModelPortaliQ99NH7.
SMRiQ99NH7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030878.

Protein family/group databases

TCDBi2.A.53.2.5. the sulfate permease (sulp) family.

PTM databases

iPTMnetiQ99NH7.
PhosphoSitePlusiQ99NH7.

Proteomic databases

PaxDbiQ99NH7.
PRIDEiQ99NH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030878; ENSMUSP00000030878; ENSMUSG00000029015.
GeneIDi80979.
KEGGimmu:80979.
UCSCiuc008wpe.2. mouse.

Organism-specific databases

CTDi375611.
MGIiMGI:1933154. Slc26a5.

Phylogenomic databases

eggNOGiKOG0236. Eukaryota.
COG0659. LUCA.
GeneTreeiENSGT00760000119026.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ99NH7.
KOiK14703.
OMAiVGNANMA.
OrthoDBiEOG091G07RT.
TreeFamiTF313784.

Miscellaneous databases

PROiQ99NH7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029015.
CleanExiMM_SLC26A5.
ExpressionAtlasiQ99NH7. baseline and differential.
GenevisibleiQ99NH7. MM.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR030282. Prestin.
IPR018045. S04_transporter_CS.
IPR011547. SLC26A/SulP_dom.
IPR001902. SLC26A/SulP_fam.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 2 hits.
PTHR11814:SF32. PTHR11814:SF32. 2 hits.
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS26A5_MOUSE
AccessioniPrimary (citable) accession number: Q99NH7
Secondary accession number(s): Q80ZB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.