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Q99NH7

- S26A5_MOUSE

UniProt

Q99NH7 - S26A5_MOUSE

Protein

Prestin

Gene

Slc26a5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. secondary active sulfate transmembrane transporter activity Source: InterPro
    3. spectrin binding Source: MGI

    GO - Biological processi

    1. regulation of cell shape Source: UniProtKB-KW
    2. regulation of membrane potential Source: MGI
    3. sensory perception of sound Source: UniProtKB-KW

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cell shape, Hearing

    Protein family/group databases

    TCDBi2.A.53.2.5. the sulfate permease (sulp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prestin
    Alternative name(s):
    Solute carrier family 26 member 5
    Gene namesi
    Name:Slc26a5
    Synonyms:Pres
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1933154. Slc26a5.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Lateral wall of outer hair cells.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. lateral plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744PrestinPRO_0000080169Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ99NH7.

    PTM databases

    PhosphoSiteiQ99NH7.

    Expressioni

    Tissue specificityi

    Expressed in the outer hair cells (OHC) of the organ of Corti of the inner ear. Also weak expression in brain and testis. Very weakly expressed in heart, spleen, muscle and lactating mammary glands.1 Publication

    Gene expression databases

    BgeeiQ99NH7.
    CleanExiMM_SLC26A5.
    GenevestigatoriQ99NH7.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000030878.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99NH7.
    SMRiQ99NH7. Positions 505-555, 638-718.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7979CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini101 – 1022ExtracellularSequence Analysis
    Topological domaini124 – 1318CytoplasmicSequence Analysis
    Topological domaini153 – 18432ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini206 – 2116CytoplasmicSequence Analysis
    Topological domaini233 – 25321ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini275 – 28612CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini308 – 33427ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini356 – 37419CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini396 – 41116ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini433 – 4419CytoplasmicSequence Analysis
    Topological domaini463 – 47917ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini501 – 744244CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei80 – 10021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei103 – 12321Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei132 – 15221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei185 – 20521Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei254 – 27421Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei287 – 30721Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei335 – 35521Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei375 – 39521Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei442 – 46221Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei480 – 50021Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini525 – 713189STASPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 STAS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0659.
    GeneTreeiENSGT00750000117367.
    HOGENOMiHOG000006546.
    HOVERGENiHBG000639.
    InParanoidiQ80ZB1.
    KOiK14703.
    OMAiQRFMPPG.
    TreeFamiTF313784.

    Family and domain databases

    Gene3Di3.30.750.24. 2 hits.
    InterProiIPR018045. S04_transporter_CS.
    IPR002645. STAS_dom.
    IPR001902. SulP_transpt.
    IPR011547. Sulph_transpt.
    [Graphical view]
    PfamiPF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view]
    SUPFAMiSSF52091. SSF52091. 2 hits.
    TIGRFAMsiTIGR00815. sulP. 1 hit.
    PROSITEiPS01130. SLC26A. 1 hit.
    PS50801. STAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99NH7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDHAEENEIP AETQRYYVER PIFSHPVLQE RLHVKDKVTE SIGDKLKQAF    50
    TCTPKKIRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA 100
    FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR 150
    LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF 200
    VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV 250
    LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 300
    TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG 350
    FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS 400
    LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG 450
    MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI 500
    YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD 550
    LYSSALKRKT GVNPALIMGA RRKAMRKYAK EVGNANVANA TVVKVDAEVD 600
    GENATKPEEE DDEVKFPPIV IKTTFPEELQ RFLPQGENVH TVILDFTQVN 650
    FVDSVGVKTL AGIVKEYGDV GIYVYLAGCS PQVVNDLTRN NFFENPALKE 700
    LLFHSIHDAV LGSQVREAMA EQEATASLPQ EDMEPNATPT TPEA 744
    Length:744
    Mass (Da):81,337
    Last modified:July 27, 2011 - v3
    Checksum:i37A2524F5E75E31B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti469 – 4691L → P in AAG59999. (PubMed:14553901)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY024359 mRNA. Translation: AAG59999.2.
    AF529192 mRNA. Translation: AAO59381.1.
    CH466586 Genomic DNA. Translation: EDL03187.1.
    CCDSiCCDS19109.1.
    RefSeqiNP_001276716.1. NM_001289787.1.
    NP_001276717.1. NM_001289788.1.
    NP_109652.3. NM_030727.5.
    XP_006535879.1. XM_006535816.1.
    UniGeneiMm.160370.

    Genome annotation databases

    EnsembliENSMUST00000030878; ENSMUSP00000030878; ENSMUSG00000029015.
    GeneIDi80979.
    KEGGimmu:80979.
    UCSCiuc008wpe.1. mouse.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Pump up the volume - Issue 22 of May 2002

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY024359 mRNA. Translation: AAG59999.2 .
    AF529192 mRNA. Translation: AAO59381.1 .
    CH466586 Genomic DNA. Translation: EDL03187.1 .
    CCDSi CCDS19109.1.
    RefSeqi NP_001276716.1. NM_001289787.1.
    NP_001276717.1. NM_001289788.1.
    NP_109652.3. NM_030727.5.
    XP_006535879.1. XM_006535816.1.
    UniGenei Mm.160370.

    3D structure databases

    ProteinModelPortali Q99NH7.
    SMRi Q99NH7. Positions 505-555, 638-718.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000030878.

    Protein family/group databases

    TCDBi 2.A.53.2.5. the sulfate permease (sulp) family.

    PTM databases

    PhosphoSitei Q99NH7.

    Proteomic databases

    PRIDEi Q99NH7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030878 ; ENSMUSP00000030878 ; ENSMUSG00000029015 .
    GeneIDi 80979.
    KEGGi mmu:80979.
    UCSCi uc008wpe.1. mouse.

    Organism-specific databases

    CTDi 375611.
    MGIi MGI:1933154. Slc26a5.

    Phylogenomic databases

    eggNOGi COG0659.
    GeneTreei ENSGT00750000117367.
    HOGENOMi HOG000006546.
    HOVERGENi HBG000639.
    InParanoidi Q80ZB1.
    KOi K14703.
    OMAi QRFMPPG.
    TreeFami TF313784.

    Miscellaneous databases

    NextBioi 350310.
    PROi Q99NH7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q99NH7.
    CleanExi MM_SLC26A5.
    Genevestigatori Q99NH7.

    Family and domain databases

    Gene3Di 3.30.750.24. 2 hits.
    InterProi IPR018045. S04_transporter_CS.
    IPR002645. STAS_dom.
    IPR001902. SulP_transpt.
    IPR011547. Sulph_transpt.
    [Graphical view ]
    Pfami PF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52091. SSF52091. 2 hits.
    TIGRFAMsi TIGR00815. sulP. 1 hit.
    PROSITEi PS01130. SLC26A. 1 hit.
    PS50801. STAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of prestin, a membrane motor protein, in the mammalian auditory and vestibular periphery."
      Dougherty G.W., Kachar B.
      Hear. Res. 184:27-40(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Organ of Corti.
    2. "Genomic characterization and expression of mouse prestin, the motor protein of outer hair cells."
      Zheng J., Long K.B., Matsuda K., Madison L.D., Ryan A., Dallos P.D.
      Mamm. Genome 14:87-96(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: CBA/CaJ.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiS26A5_MOUSE
    AccessioniPrimary (citable) accession number: Q99NH7
    Secondary accession number(s): Q80ZB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3