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Q99NH7

- S26A5_MOUSE

UniProt

Q99NH7 - S26A5_MOUSE

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Protein
Prestin
Gene
Slc26a5, Pres
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site By similarity.

GO - Molecular functioni

  1. protein binding Source: MGI
  2. secondary active sulfate transmembrane transporter activity Source: InterPro
  3. spectrin binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. regulation of cell shape Source: UniProtKB-KW
  2. regulation of membrane potential Source: MGI
  3. sensory perception of sound Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell shape, Hearing

Protein family/group databases

TCDBi2.A.53.2.5. the sulfate permease (sulp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene namesi
Name:Slc26a5
Synonyms:Pres
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1933154. Slc26a5.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity
Note: Lateral wall of outer hair cells By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7979Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei80 – 10021Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini101 – 1022Extracellular Reviewed prediction
Transmembranei103 – 12321Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini124 – 1318Cytoplasmic Reviewed prediction
Transmembranei132 – 15221Helical; Name=3; Reviewed prediction
Add
BLAST
Topological domaini153 – 18432Extracellular Reviewed prediction
Add
BLAST
Transmembranei185 – 20521Helical; Name=4; Reviewed prediction
Add
BLAST
Topological domaini206 – 2116Cytoplasmic Reviewed prediction
Transmembranei212 – 23221Helical; Name=5; Reviewed prediction
Add
BLAST
Topological domaini233 – 25321Extracellular Reviewed prediction
Add
BLAST
Transmembranei254 – 27421Helical; Name=6; Reviewed prediction
Add
BLAST
Topological domaini275 – 28612Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei287 – 30721Helical; Name=7; Reviewed prediction
Add
BLAST
Topological domaini308 – 33427Extracellular Reviewed prediction
Add
BLAST
Transmembranei335 – 35521Helical; Name=8; Reviewed prediction
Add
BLAST
Topological domaini356 – 37419Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei375 – 39521Helical; Name=9; Reviewed prediction
Add
BLAST
Topological domaini396 – 41116Extracellular Reviewed prediction
Add
BLAST
Transmembranei412 – 43221Helical; Name=10; Reviewed prediction
Add
BLAST
Topological domaini433 – 4419Cytoplasmic Reviewed prediction
Transmembranei442 – 46221Helical; Name=11; Reviewed prediction
Add
BLAST
Topological domaini463 – 47917Extracellular Reviewed prediction
Add
BLAST
Transmembranei480 – 50021Helical; Name=12; Reviewed prediction
Add
BLAST
Topological domaini501 – 744244Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. lateral plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Prestin
PRO_0000080169Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi163 – 1631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi166 – 1661N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ99NH7.

PTM databases

PhosphoSiteiQ99NH7.

Expressioni

Tissue specificityi

Expressed in the outer hair cells (OHC) of the organ of Corti of the inner ear. Also weak expression in brain and testis. Very weakly expressed in heart, spleen, muscle and lactating mammary glands.1 Publication

Gene expression databases

BgeeiQ99NH7.
CleanExiMM_SLC26A5.
GenevestigatoriQ99NH7.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030878.

Structurei

3D structure databases

ProteinModelPortaliQ99NH7.
SMRiQ99NH7. Positions 505-555, 638-718.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini525 – 713189STAS
Add
BLAST

Sequence similaritiesi

Contains 1 STAS domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0659.
GeneTreeiENSGT00750000117367.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ80ZB1.
KOiK14703.
OMAiQRFMPPG.
TreeFamiTF313784.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view]
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99NH7-1 [UniParc]FASTAAdd to Basket

« Hide

MDHAEENEIP AETQRYYVER PIFSHPVLQE RLHVKDKVTE SIGDKLKQAF    50
TCTPKKIRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA 100
FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR 150
LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF 200
VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV 250
LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 300
TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG 350
FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS 400
LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG 450
MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI 500
YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD 550
LYSSALKRKT GVNPALIMGA RRKAMRKYAK EVGNANVANA TVVKVDAEVD 600
GENATKPEEE DDEVKFPPIV IKTTFPEELQ RFLPQGENVH TVILDFTQVN 650
FVDSVGVKTL AGIVKEYGDV GIYVYLAGCS PQVVNDLTRN NFFENPALKE 700
LLFHSIHDAV LGSQVREAMA EQEATASLPQ EDMEPNATPT TPEA 744
Length:744
Mass (Da):81,337
Last modified:July 27, 2011 - v3
Checksum:i37A2524F5E75E31B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti469 – 4691L → P in AAG59999. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY024359 mRNA. Translation: AAG59999.2.
AF529192 mRNA. Translation: AAO59381.1.
CH466586 Genomic DNA. Translation: EDL03187.1.
CCDSiCCDS19109.1.
RefSeqiNP_001276716.1. NM_001289787.1.
NP_001276717.1. NM_001289788.1.
NP_109652.3. NM_030727.5.
XP_006535879.1. XM_006535816.1.
UniGeneiMm.160370.

Genome annotation databases

EnsembliENSMUST00000030878; ENSMUSP00000030878; ENSMUSG00000029015.
GeneIDi80979.
KEGGimmu:80979.
UCSCiuc008wpe.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY024359 mRNA. Translation: AAG59999.2 .
AF529192 mRNA. Translation: AAO59381.1 .
CH466586 Genomic DNA. Translation: EDL03187.1 .
CCDSi CCDS19109.1.
RefSeqi NP_001276716.1. NM_001289787.1.
NP_001276717.1. NM_001289788.1.
NP_109652.3. NM_030727.5.
XP_006535879.1. XM_006535816.1.
UniGenei Mm.160370.

3D structure databases

ProteinModelPortali Q99NH7.
SMRi Q99NH7. Positions 505-555, 638-718.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000030878.

Protein family/group databases

TCDBi 2.A.53.2.5. the sulfate permease (sulp) family.

PTM databases

PhosphoSitei Q99NH7.

Proteomic databases

PRIDEi Q99NH7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030878 ; ENSMUSP00000030878 ; ENSMUSG00000029015 .
GeneIDi 80979.
KEGGi mmu:80979.
UCSCi uc008wpe.1. mouse.

Organism-specific databases

CTDi 375611.
MGIi MGI:1933154. Slc26a5.

Phylogenomic databases

eggNOGi COG0659.
GeneTreei ENSGT00750000117367.
HOGENOMi HOG000006546.
HOVERGENi HBG000639.
InParanoidi Q80ZB1.
KOi K14703.
OMAi QRFMPPG.
TreeFami TF313784.

Miscellaneous databases

NextBioi 350310.
PROi Q99NH7.
SOURCEi Search...

Gene expression databases

Bgeei Q99NH7.
CleanExi MM_SLC26A5.
Genevestigatori Q99NH7.

Family and domain databases

Gene3Di 3.30.750.24. 2 hits.
InterProi IPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view ]
Pfami PF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view ]
SUPFAMi SSF52091. SSF52091. 2 hits.
TIGRFAMsi TIGR00815. sulP. 1 hit.
PROSITEi PS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of prestin, a membrane motor protein, in the mammalian auditory and vestibular periphery."
    Dougherty G.W., Kachar B.
    Hear. Res. 184:27-40(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Organ of Corti.
  2. "Genomic characterization and expression of mouse prestin, the motor protein of outer hair cells."
    Zheng J., Long K.B., Matsuda K., Madison L.D., Ryan A., Dallos P.D.
    Mamm. Genome 14:87-96(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: CBA/CaJ.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiS26A5_MOUSE
AccessioniPrimary (citable) accession number: Q99NH7
Secondary accession number(s): Q80ZB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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