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Q99NH7 (S26A5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene names
Name:Slc26a5
Synonyms:Pres
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Note: Lateral wall of outer hair cells By similarity.

Tissue specificity

Expressed in the outer hair cells (OHC) of the organ of Corti of the inner ear. Also weak expression in brain and testis. Very weakly expressed in heart, spleen, muscle and lactating mammary glands. Ref.2

Sequence similarities

Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. [View classification]

Contains 1 STAS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 744744Prestin
PRO_0000080169

Regions

Topological domain1 – 7979Cytoplasmic Potential
Transmembrane80 – 10021Helical; Name=1; Potential
Topological domain101 – 1022Extracellular Potential
Transmembrane103 – 12321Helical; Name=2; Potential
Topological domain124 – 1318Cytoplasmic Potential
Transmembrane132 – 15221Helical; Name=3; Potential
Topological domain153 – 18432Extracellular Potential
Transmembrane185 – 20521Helical; Name=4; Potential
Topological domain206 – 2116Cytoplasmic Potential
Transmembrane212 – 23221Helical; Name=5; Potential
Topological domain233 – 25321Extracellular Potential
Transmembrane254 – 27421Helical; Name=6; Potential
Topological domain275 – 28612Cytoplasmic Potential
Transmembrane287 – 30721Helical; Name=7; Potential
Topological domain308 – 33427Extracellular Potential
Transmembrane335 – 35521Helical; Name=8; Potential
Topological domain356 – 37419Cytoplasmic Potential
Transmembrane375 – 39521Helical; Name=9; Potential
Topological domain396 – 41116Extracellular Potential
Transmembrane412 – 43221Helical; Name=10; Potential
Topological domain433 – 4419Cytoplasmic Potential
Transmembrane442 – 46221Helical; Name=11; Potential
Topological domain463 – 47917Extracellular Potential
Transmembrane480 – 50021Helical; Name=12; Potential
Topological domain501 – 744244Cytoplasmic Potential
Domain525 – 713189STAS

Amino acid modifications

Glycosylation1631N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4691L → P in AAG59999. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q99NH7 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 37A2524F5E75E31B

FASTA74481,337
        10         20         30         40         50         60 
MDHAEENEIP AETQRYYVER PIFSHPVLQE RLHVKDKVTE SIGDKLKQAF TCTPKKIRNI 

        70         80         90        100        110        120 
IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA FAMLAAVPPV FGLYSSFYPV 

       130        140        150        160        170        180 
IMYCFFGTSR HISIGPFAVI SLMIGGVAVR LVPDDIVIPG GVNATNGTEA RDALRVKVAM 

       190        200        210        220        230        240 
SVTLLSGIIQ FCLGVCRFGF VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI 

       250        260        270        280        290        300 
FSVVYSTVAV LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 

       310        320        330        340        350        360 
TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG FSVTISMAKT 

       370        380        390        400        410        420 
LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS LVQEGTGGKT QLAGCLASLM 

       430        440        450        460        470        480 
ILLVILATGF LFESLPQAVL SAIVIVNLKG MFMQFSDLPF FWRTSKIELT IWLTTFVSSL 

       490        500        510        520        530        540 
FLGLDYGLIT AVIIALLTVI YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI 

       550        560        570        580        590        600 
NAPIYYANSD LYSSALKRKT GVNPALIMGA RRKAMRKYAK EVGNANVANA TVVKVDAEVD 

       610        620        630        640        650        660 
GENATKPEEE DDEVKFPPIV IKTTFPEELQ RFLPQGENVH TVILDFTQVN FVDSVGVKTL 

       670        680        690        700        710        720 
AGIVKEYGDV GIYVYLAGCS PQVVNDLTRN NFFENPALKE LLFHSIHDAV LGSQVREAMA 

       730        740 
EQEATASLPQ EDMEPNATPT TPEA 

« Hide

References

« Hide 'large scale' references
[1]"Expression of prestin, a membrane motor protein, in the mammalian auditory and vestibular periphery."
Dougherty G.W., Kachar B.
Hear. Res. 184:27-40(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Organ of Corti.
[2]"Genomic characterization and expression of mouse prestin, the motor protein of outer hair cells."
Zheng J., Long K.B., Matsuda K., Madison L.D., Ryan A., Dallos P.D.
Mamm. Genome 14:87-96(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: CBA/CaJ.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Web resources

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY024359 mRNA. Translation: AAG59999.2.
AF529192 mRNA. Translation: AAO59381.1.
CH466586 Genomic DNA. Translation: EDL03187.1.
RefSeqNP_109652.3. NM_030727.5.
XP_006535879.1. XM_006535816.1.
UniGeneMm.160370.

3D structure databases

ProteinModelPortalQ99NH7.
SMRQ99NH7. Positions 505-718.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000030878.

Protein family/group databases

TCDB2.A.53.2.5. the sulfate permease (sulp) family.

PTM databases

PhosphoSiteQ99NH7.

Proteomic databases

PRIDEQ99NH7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030878; ENSMUSP00000030878; ENSMUSG00000029015.
GeneID80979.
KEGGmmu:80979.
UCSCuc008wpe.1. mouse.

Organism-specific databases

CTD375611.
MGIMGI:1933154. Slc26a5.

Phylogenomic databases

eggNOGCOG0659.
GeneTreeENSGT00750000117367.
HOGENOMHOG000006546.
HOVERGENHBG000639.
InParanoidQ80ZB1.
KOK14703.
OMAEVGNANM.
TreeFamTF313784.

Gene expression databases

BgeeQ99NH7.
CleanExMM_SLC26A5.
GenevestigatorQ99NH7.

Family and domain databases

Gene3D3.30.750.24. 2 hits.
InterProIPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view]
PfamPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMSSF52091. SSF52091. 2 hits.
TIGRFAMsTIGR00815. sulP. 1 hit.
PROSITEPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio350310.
PROQ99NH7.
SOURCESearch...

Entry information

Entry nameS26A5_MOUSE
AccessionPrimary (citable) accession number: Q99NH7
Secondary accession number(s): Q80ZB1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot