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Q99NH7

- S26A5_MOUSE

UniProt

Q99NH7 - S26A5_MOUSE

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Protein

Prestin

Gene

Slc26a5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site (By similarity).By similarity

GO - Molecular functioni

  1. secondary active sulfate transmembrane transporter activity Source: InterPro
  2. spectrin binding Source: MGI

GO - Biological processi

  1. regulation of cell shape Source: UniProtKB-KW
  2. regulation of membrane potential Source: MGI
  3. sensory perception of sound Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell shape, Hearing

Protein family/group databases

TCDBi2.A.53.2.5. the sulfate permease (sulp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene namesi
Name:Slc26a5
Synonyms:Pres
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1933154. Slc26a5.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Lateral wall of outer hair cells.By similarity

GO - Cellular componenti

  1. basolateral plasma membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. lateral plasma membrane Source: MGI
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744PrestinPRO_0000080169Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ99NH7.

PTM databases

PhosphoSiteiQ99NH7.

Expressioni

Tissue specificityi

Expressed in the outer hair cells (OHC) of the organ of Corti of the inner ear. Also weak expression in brain and testis. Very weakly expressed in heart, spleen, muscle and lactating mammary glands.1 Publication

Gene expression databases

BgeeiQ99NH7.
CleanExiMM_SLC26A5.
GenevestigatoriQ99NH7.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030878.

Structurei

3D structure databases

ProteinModelPortaliQ99NH7.
SMRiQ99NH7. Positions 505-555, 638-718.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7979CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini101 – 1022ExtracellularSequence Analysis
Topological domaini124 – 1318CytoplasmicSequence Analysis
Topological domaini153 – 18432ExtracellularSequence AnalysisAdd
BLAST
Topological domaini206 – 2116CytoplasmicSequence Analysis
Topological domaini233 – 25321ExtracellularSequence AnalysisAdd
BLAST
Topological domaini275 – 28612CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini308 – 33427ExtracellularSequence AnalysisAdd
BLAST
Topological domaini356 – 37419CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini396 – 41116ExtracellularSequence AnalysisAdd
BLAST
Topological domaini433 – 4419CytoplasmicSequence Analysis
Topological domaini463 – 47917ExtracellularSequence AnalysisAdd
BLAST
Topological domaini501 – 744244CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei80 – 10021Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei103 – 12321Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei132 – 15221Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei185 – 20521Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei212 – 23221Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei254 – 27421Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei287 – 30721Helical; Name=7Sequence AnalysisAdd
BLAST
Transmembranei335 – 35521Helical; Name=8Sequence AnalysisAdd
BLAST
Transmembranei375 – 39521Helical; Name=9Sequence AnalysisAdd
BLAST
Transmembranei412 – 43221Helical; Name=10Sequence AnalysisAdd
BLAST
Transmembranei442 – 46221Helical; Name=11Sequence AnalysisAdd
BLAST
Transmembranei480 – 50021Helical; Name=12Sequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini525 – 713189STASPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0659.
GeneTreeiENSGT00760000119026.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ99NH7.
KOiK14703.
OMAiQRFMPPG.
TreeFamiTF313784.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view]
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99NH7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDHAEENEIP AETQRYYVER PIFSHPVLQE RLHVKDKVTE SIGDKLKQAF
60 70 80 90 100
TCTPKKIRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA
110 120 130 140 150
FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR
160 170 180 190 200
LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF
210 220 230 240 250
VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV
260 270 280 290 300
LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG
310 320 330 340 350
TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG
360 370 380 390 400
FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS
410 420 430 440 450
LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG
460 470 480 490 500
MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI
510 520 530 540 550
YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD
560 570 580 590 600
LYSSALKRKT GVNPALIMGA RRKAMRKYAK EVGNANVANA TVVKVDAEVD
610 620 630 640 650
GENATKPEEE DDEVKFPPIV IKTTFPEELQ RFLPQGENVH TVILDFTQVN
660 670 680 690 700
FVDSVGVKTL AGIVKEYGDV GIYVYLAGCS PQVVNDLTRN NFFENPALKE
710 720 730 740
LLFHSIHDAV LGSQVREAMA EQEATASLPQ EDMEPNATPT TPEA
Length:744
Mass (Da):81,337
Last modified:July 27, 2011 - v3
Checksum:i37A2524F5E75E31B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti469 – 4691L → P in AAG59999. (PubMed:14553901)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY024359 mRNA. Translation: AAG59999.2.
AF529192 mRNA. Translation: AAO59381.1.
CH466586 Genomic DNA. Translation: EDL03187.1.
CCDSiCCDS19109.1.
RefSeqiNP_001276716.1. NM_001289787.1.
NP_001276717.1. NM_001289788.1.
NP_109652.3. NM_030727.5.
XP_006535879.1. XM_006535816.1.
UniGeneiMm.160370.

Genome annotation databases

EnsembliENSMUST00000030878; ENSMUSP00000030878; ENSMUSG00000029015.
GeneIDi80979.
KEGGimmu:80979.
UCSCiuc008wpe.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY024359 mRNA. Translation: AAG59999.2 .
AF529192 mRNA. Translation: AAO59381.1 .
CH466586 Genomic DNA. Translation: EDL03187.1 .
CCDSi CCDS19109.1.
RefSeqi NP_001276716.1. NM_001289787.1.
NP_001276717.1. NM_001289788.1.
NP_109652.3. NM_030727.5.
XP_006535879.1. XM_006535816.1.
UniGenei Mm.160370.

3D structure databases

ProteinModelPortali Q99NH7.
SMRi Q99NH7. Positions 505-555, 638-718.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000030878.

Protein family/group databases

TCDBi 2.A.53.2.5. the sulfate permease (sulp) family.

PTM databases

PhosphoSitei Q99NH7.

Proteomic databases

PRIDEi Q99NH7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030878 ; ENSMUSP00000030878 ; ENSMUSG00000029015 .
GeneIDi 80979.
KEGGi mmu:80979.
UCSCi uc008wpe.1. mouse.

Organism-specific databases

CTDi 375611.
MGIi MGI:1933154. Slc26a5.

Phylogenomic databases

eggNOGi COG0659.
GeneTreei ENSGT00760000119026.
HOGENOMi HOG000006546.
HOVERGENi HBG000639.
InParanoidi Q99NH7.
KOi K14703.
OMAi QRFMPPG.
TreeFami TF313784.

Miscellaneous databases

NextBioi 350310.
PROi Q99NH7.
SOURCEi Search...

Gene expression databases

Bgeei Q99NH7.
CleanExi MM_SLC26A5.
Genevestigatori Q99NH7.

Family and domain databases

Gene3Di 3.30.750.24. 2 hits.
InterProi IPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view ]
Pfami PF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view ]
SUPFAMi SSF52091. SSF52091. 2 hits.
TIGRFAMsi TIGR00815. sulP. 1 hit.
PROSITEi PS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of prestin, a membrane motor protein, in the mammalian auditory and vestibular periphery."
    Dougherty G.W., Kachar B.
    Hear. Res. 184:27-40(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Organ of Corti.
  2. "Genomic characterization and expression of mouse prestin, the motor protein of outer hair cells."
    Zheng J., Long K.B., Matsuda K., Madison L.D., Ryan A., Dallos P.D.
    Mamm. Genome 14:87-96(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: CBA/CaJ.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiS26A5_MOUSE
AccessioniPrimary (citable) accession number: Q99NH7
Secondary accession number(s): Q80ZB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3