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Protein

Helicase ARIP4

Gene

Rad54l2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro. Acts as an AR-coregulator in Sertoli cells.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Enzyme regulationi

Enzyme activity is enhanced by dsDNA (double-stranded DNA) and ssDNA (single-stranded DNA).1 Publication

Kineticsi

  1. KM=40 nM for DNA1 Publication
  2. KM=25 µM for ATP1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi304 – 311ATPCurated8

    GO - Molecular functioni

    • ATPase activity Source: MGI
    • ATP binding Source: MGI
    • DNA binding Source: UniProtKB-KW
    • helicase activity Source: UniProtKB-KW
    • protein kinase binding Source: MGI
    • transcription cofactor activity Source: MGI

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Helicase ARIP4 (EC:3.6.4.12)
    Alternative name(s):
    Androgen receptor-interacting protein 4
    RAD54-like protein 2
    Steroid receptor-interacting SNF2 domain-containing protein-like
    Gene namesi
    Name:Rad54l2
    Synonyms:Arip4, Srisnf2l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Unplaced

    Organism-specific databases

    MGIiMGI:1933196. Rad54l2.

    Subcellular locationi

    GO - Cellular componenti

    • nuclear speck Source: MGI
    • nucleus Source: MGI
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Death by E11.5. At E9.5 and E10.5, almost all major tissues are proportionally smaller, and the neural tube is shrunk in some embryos. Dramatically reduced cell proliferation and increased apoptosis are observed in E9.5 and E10.5 Embryonic fibroblasts stop to grow after 2 or 3 passages and exhibit increased apoptosis and decreased DNA synthesis compared with wild-type.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi310K → A: Abolishes ATP-binding. 1 Publication1
    Mutagenesisi361K → E: Decreased sumoylation; when associated with E-573; E-664; E-935; E-961 and E-1013. 1 Publication1
    Mutagenesisi462 – 463DE → AA: Abolishes ATPase activity. 1 Publication2
    Mutagenesisi573K → E: Decreased sumoylation; when associated with E-361; E-664; E-935; E-961 and E-1013. 1 Publication1
    Mutagenesisi664K → E: Decreased sumoylation; when associated with E-361; E-573; E-935; E-961 and E-1013. 1 Publication1
    Mutagenesisi935K → E: Decreased sumoylation; when associated with E-361; E-573; E-664; E-961 and E-1013. 1 Publication1
    Mutagenesisi961K → E: Decreased sumoylation; when associated with E-361; E-573; E-664; E-935 and E-1013. 1 Publication1
    Mutagenesisi1013K → E: Decreased sumoylation; when associated with E-361; E-573; E-664; E-935 and E-961. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003157821 – 1466Helicase ARIP4Add BLAST1466

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki1017Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
    Modified residuei1168PhosphoserineCombined sources1
    Modified residuei1171PhosphoserineCombined sources1
    Modified residuei1259PhosphothreonineBy similarity1

    Post-translational modificationi

    Sumoylated.1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    EPDiQ99NG0.
    MaxQBiQ99NG0.
    PaxDbiQ99NG0.
    PRIDEiQ99NG0.

    PTM databases

    iPTMnetiQ99NG0.
    PhosphoSitePlusiQ99NG0.

    Expressioni

    Tissue specificityi

    Expressed at relatively low level, with highest expression in testis, liver and kidney. In brain, it is expressed in hippocampal and cerebellar neurons. In testis, it is present at high level in Sertoli cell nuclei. Also present in Leydig cell (at protein level).2 Publications

    Developmental stagei

    Mainly expressed in the neural tube and limb buds during early embryonic development. Also present in testis: at the onset of spermatogenesis, it is expressed in spermatogonia, pachytene, and diplotene spermatocytes. In Sertoli cells it is expressed in a stage-dependent manner, with high expression levels at stages II-VI and VII-VIII.2 Publications

    Gene expression databases

    CleanExiMM_RAD54L2.

    Interactioni

    Subunit structurei

    Interacts with AR via its N-terminus. Interacts with DYRK1A. Binds DNA and mononucleosomes, but does not seem to form large multiprotein complexes.3 Publications

    GO - Molecular functioni

    • protein kinase binding Source: MGI

    Protein-protein interaction databases

    BioGridi219861. 1 interactor.
    STRINGi10090.ENSMUSP00000045454.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99NG0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini291 – 511Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST221
    Domaini727 – 895Helicase C-terminalPROSITE-ProRule annotationAdd BLAST169

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi462 – 465DEAH box4
    Motifi550 – 554LXXLL motif 15
    Motifi1328 – 1332LXXLL motif 25

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi18 – 42Glu-richAdd BLAST25
    Compositional biasi222 – 228Poly-Glu7

    Domaini

    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to be important for the association with nuclear receptors.By similarity

    Sequence similaritiesi

    Belongs to the SNF2/RAD54 helicase family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiKOG1015. Eukaryota.
    COG0553. LUCA.
    HOGENOMiHOG000168429.
    HOVERGENiHBG104283.
    InParanoidiQ99NG0.
    KOiK10876.
    PhylomeDBiQ99NG0.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99NG0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSDESASGSD PDLDPDVELE DEEEEEEEEE VAVEEHDRDD EEGLLDDTSL
    60 70 80 90 100
    EGMCGTEHAQ LGEDGQRPPR CTSTTSSQSE PSEQLRHQGK ILASEDPKKK
    110 120 130 140 150
    RAQKPSHMRR NIRKLLREDQ LEPVTKAAQQ EELERRKRLE QQRKEYAAPI
    160 170 180 190 200
    PTVPLEFLPE EIVLRASDGP QLPPRVLAQE VICLDSSSGS EDEKSSRDEV
    210 220 230 240 250
    IELSSGEEDT LHIVDSSESV SEEDEEEEKG GTHVNDALNQ HDALGRVLVN
    260 270 280 290 300
    LNHPPEEENV FLAPQLARAV KPHQIGGIRF LYDNLVESLE RFKTSSGFGC
    310 320 330 340 350
    ILAHSMGLGK TLQVISFIDV LFRHTPAKTV LAIVPVNTLQ NWLAEFNMWL
    360 370 380 390 400
    PAPEALPADS KPEEVQPRFF KVHILNDEHK TVASRAKVTA DWVSEGGVLL
    410 420 430 440 450
    MGYEMYRLLT LKKSLATSRP KKTKKRSHPV IIDLDEEDRQ QEFRREFEKA
    460 470 480 490 500
    LCRPGPDVVI CDEGHRIKNC QASTSQALKN IRSRRRVVLT GYPLQNNLIE
    510 520 530 540 550
    YWCMVDFVRP DFLGTRQEFS NMFERPILNG QCIDSTPQDV RLMRYRSHVL
    560 570 580 590 600
    HSLLEGFVQR RGHTVLKIHL PAKEENVILV RLSQIQRDLY TQFMDRFRDC
    610 620 630 640 650
    GTSGWLGLNP LKAFCVCCKI WNHPDVLYEA LQKENLANEQ DLDVEELGSA
    660 670 680 690 700
    GTSARCPPHG TKVKGEDSAL PSSMGEATNS KFLQGVGFNP FQERGNNIVT
    710 720 730 740 750
    YEWAKELLTN YQTGVLENSP KMVLLFHLIE ESVKLGDKIL VFSQSLSTLA
    760 770 780 790 800
    LIEEFLGKRD MPCLPGAEGQ GTQKWVRNVS YFRLDGSTPA FERERLINQF
    810 820 830 840 850
    NDPSNLTTWL FLLSTRAGCL GVNLIGANRV VVFDASWNPC HDAQAVCRVY
    860 870 880 890 900
    RYGQKKPCHI YRLVADYTLE KKIYDRQISK QGMSDRVVDD LNPMLNFTRK
    910 920 930 940 950
    EVENLLHFVE KEPAPQTSLD IKGIKESVLQ LACLKYPHLI TKEPFEHESL
    960 970 980 990 1000
    LLNRKDHKLT KAEKKAAKKS YEEDKRTSVP YTRPSYAQYY PASDQSLTSI
    1010 1020 1030 1040 1050
    PAFSQRNWQP TLKGDEKPVA SVRPVQSTPI PMMPRHVPLS GGVSSASSTN
    1060 1070 1080 1090 1100
    TSMNFPINYL QRAGVLVQKV VTTTDIVIPG LNSSTDVQAR INAGESIHII
    1110 1120 1130 1140 1150
    RGTKGTYIRT SDGRIFAVRA TGKPKAPEDG RMAASGSQGP SLASTSNGRH
    1160 1170 1180 1190 1200
    SASSPKAPDP EGLARPVSPD SPEIISELQQ YADVAAARES RQSSPSISAA
    1210 1220 1230 1240 1250
    LPGPPGQLMD NSTIPGTALG TEPCLGGHCL NSSLLVTGQP SGGRHPVLDL
    1260 1270 1280 1290 1300
    RGHKRKLATP SVTQESIRRR SRKGHLPAPV QPYEHGYPVS GGFAMPPVSL
    1310 1320 1330 1340 1350
    NHNLTTPFTS QAGENSLFMG SNPSYYQLSN LLADARLVFP VTTDPLVPAG
    1360 1370 1380 1390 1400
    PVSSSSTATS VTASNPSFML NPSVPGMLPS YSLPFSQPLL SEPRMFAPFP
    1410 1420 1430 1440 1450
    SPGLPSNLSR GVSVYPGYMS PHAGYPAGGL LRSQVPPFDS HEVAEVGFSS
    1460
    NDDEDKDDDV IEVTGK
    Length:1,466
    Mass (Da):162,540
    Last modified:June 1, 2001 - v1
    Checksum:iD5945AD802B03D12
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti1197I → V in BAE25404 (PubMed:16141072).Curated1
    Sequence conflicti1267I → V in BAE25404 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ132389 mRNA. Translation: CAC24703.1.
    BC133714 mRNA. Translation: AAI33715.1.
    AK143506 mRNA. Translation: BAE25404.1.
    RefSeqiNP_109655.2. NM_030730.2.
    UniGeneiMm.246689.

    Genome annotation databases

    GeneIDi81000.
    KEGGimmu:81000.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ132389 mRNA. Translation: CAC24703.1.
    BC133714 mRNA. Translation: AAI33715.1.
    AK143506 mRNA. Translation: BAE25404.1.
    RefSeqiNP_109655.2. NM_030730.2.
    UniGeneiMm.246689.

    3D structure databases

    ProteinModelPortaliQ99NG0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi219861. 1 interactor.
    STRINGi10090.ENSMUSP00000045454.

    PTM databases

    iPTMnetiQ99NG0.
    PhosphoSitePlusiQ99NG0.

    Proteomic databases

    EPDiQ99NG0.
    MaxQBiQ99NG0.
    PaxDbiQ99NG0.
    PRIDEiQ99NG0.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi81000.
    KEGGimmu:81000.

    Organism-specific databases

    CTDi23132.
    MGIiMGI:1933196. Rad54l2.

    Phylogenomic databases

    eggNOGiKOG1015. Eukaryota.
    COG0553. LUCA.
    HOGENOMiHOG000168429.
    HOVERGENiHBG104283.
    InParanoidiQ99NG0.
    KOiK10876.
    PhylomeDBiQ99NG0.

    Miscellaneous databases

    ChiTaRSiRad54l2. mouse.
    PROiQ99NG0.
    SOURCEiSearch...

    Gene expression databases

    CleanExiMM_RAD54L2.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiARIP4_MOUSE
    AccessioniPrimary (citable) accession number: Q99NG0
    Secondary accession number(s): Q3UPJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 1, 2001
    Last modified: November 2, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.