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Protein

Regulating synaptic membrane exocytosis protein 1

Gene

Rims1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rab effector involved in exocytosis (PubMed:11797009). May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity (PubMed:11797009). Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 193FYVE-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • ion channel binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: MGI
  • Rab GTPase binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • calcium ion-regulated exocytosis of neurotransmitter Source: MGI
  • intracellular protein transport Source: InterPro
  • neurotransmitter secretion Source: MGI
  • positive regulation of dendrite extension Source: MGI
  • positive regulation of excitatory postsynaptic potential Source: ParkinsonsUK-UCL
  • positive regulation of gene expression Source: ParkinsonsUK-UCL
  • positive regulation of inhibitory postsynaptic potential Source: ParkinsonsUK-UCL
  • protein complex assembly Source: MGI
  • regulation of long-term neuronal synaptic plasticity Source: MGI
  • regulation of membrane potential Source: MGI
  • regulation of synaptic vesicle exocytosis Source: GO_Central
  • spontaneous neurotransmitter secretion Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Differentiation, Exocytosis, Neurotransmitter transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Regulating synaptic membrane exocytosis protein 1
Alternative name(s):
Rab-3-interacting molecule 1
Short name:
RIM 1
Rab-3-interacting protein 1
Gene namesi
Name:Rims1
Synonyms:Kiaa0340, Rab3ip1, Rim1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2152971. Rims1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • plasma membrane Source: ParkinsonsUK-UCL
  • postsynaptic density Source: MGI
  • presynaptic active zone Source: GO_Central
  • presynaptic membrane Source: UniProtKB
  • synapse Source: MGI
  • vesicle Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33R → G: Abolishes interaction with RAB3A. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001901991 – 1463Regulating synaptic membrane exocytosis protein 1Add BLAST1463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei413PhosphoserineBy similarity1
Modified residuei563PhosphoserineCombined sources1
Modified residuei566PhosphoserineCombined sources1
Modified residuei716PhosphoserineCombined sources1
Modified residuei812PhosphoserineBy similarity1
Modified residuei866PhosphoserineCombined sources1
Modified residuei1023PhosphoserineCombined sources1
Modified residuei1025PhosphothreonineBy similarity1
Modified residuei1027PhosphoserineCombined sources1
Modified residuei1079PhosphoserineBy similarity1
Modified residuei1081PhosphoserineBy similarity1
Modified residuei1082PhosphoserineBy similarity1
Modified residuei1110PhosphoserineCombined sources1
Modified residuei1111PhosphoserineCombined sources1
Modified residuei1113PhosphoserineCombined sources1
Modified residuei1187PhosphoserineBy similarity1
Modified residuei1448PhosphoserineCombined sources1
Modified residuei1451PhosphoserineCombined sources1
Modified residuei1454PhosphoserineBy similarity1
Modified residuei1463PhosphoserineBy similarity1
Isoform 7 (identifier: Q99NE5-7)
Modified residuei413PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by BRSK1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiQ99NE5.
PRIDEiQ99NE5.

PTM databases

iPTMnetiQ99NE5.
PhosphoSitePlusiQ99NE5.

Expressioni

Gene expression databases

CleanExiMM_RIMS1.

Interactioni

Subunit structurei

Binds SNAP25, SYT1 and CACNA1B. Interaction with SYT1 is enhanced by calcium ions. Interaction with SNAP25 is weaker in the presence of calcium ions. Interacts with TSPOAP1 and RIMBP2; interacts with PPFIA3 and PPFIA4. Interacts with ERC1 (By similarity). Interacts with RAB3A, RAB3B and RAB3D that have been activated by GTP-binding. Interacts with RAB3C, RAB10, RAB26 AND RAB37. Binds UNC13A.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Fbxl20Q9CZV84EBI-775541,EBI-1551033

GO - Molecular functioni

  • ion channel binding Source: MGI
  • Rab GTPase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi228025. 9 interactors.
IntActiQ99NE5. 2 interactors.
MINTiMINT-1210408.

Structurei

3D structure databases

ProteinModelPortaliQ99NE5.
SMRiQ99NE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 205RabBDPROSITE-ProRule annotationAdd BLAST184
Domaini440 – 526PDZPROSITE-ProRule annotationAdd BLAST87
Domaini579 – 685C2 1PROSITE-ProRule annotationAdd BLAST107
Domaini1309 – 1411C2 2PROSITE-ProRule annotationAdd BLAST103

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 193FYVE-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG058147.
InParanoidiQ99NE5.
KOiK15291.
PhylomeDBiQ99NE5.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.40.150. 2 hits.
3.30.40.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR001478. PDZ.
IPR010911. Rab_BD.
IPR030630. RIM1.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR12157:SF19. PTHR12157:SF19. 2 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEiPS50004. C2. 2 hits.
PS50106. PDZ. 1 hit.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: Q99NE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE
60 70 80 90 100
EAMLKCVVRD MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG
110 120 130 140 150
PDRNWRLHQQ FESYKEQVRK IGEEARRYQG EHKDDAPTCG ICHKTKFADG
160 170 180 190 200
CGHLCSYCRT KFCARCGGRV SLRSNNEDKV VMWVCNLCRK QQEILTKSGA
210 220 230 240 250
WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR SQTPLSTAAV
260 270 280 290 300
SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS
310 320 330 340 350
EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP
360 370 380 390 400
DRLEKREDGR VAEDEKQRKE EEGVSTPEYT SCEDVELESE SVSEKGDLDY
410 420 430 440 450
WLDPATWHSR ETSPISSHPV TWQPSKEGDR LIGRVILNKR TTMPKESGAL
460 470 480 490 500
LGLKVVGGKM TDLGRLGAFI TKVKKGSLAD VVGHLRAGDE VLEWNGKPLP
510 520 530 540 550
GATNEEVYNI ILESKSEPQV EIIVSRPIGD IPRIPESSHP PLESSSSSFE
560 570 580 590 600
SQKMERPSIS VISPTSPGAL KDAPQVLPGQ LSVKLWYDKV GHQLIVNVLQ
610 620 630 640 650
ATDLPPRVDG RPRNPYVKMY FLPDRSDKSK RRTKTVKKLL EPKWNQTFVY
660 670 680 690 700
SHVHRRDFRE RMLEITVWDQ PRVQDEESEF LGEILIELET ALLDDEPHWY
710 720 730 740 750
KLQTHDESSL PLPQPSPFMP RRHIHGESSS KKLQRSQRIS DSDISDYEVD
760 770 780 790 800
DGIGVVPPVG YRASARESKA TTLTVPEQQR TTHHRSRSVS PHRGDDQGRP
810 820 830 840 850
RSRLPNVPLQ RSLDEIHPTR RSRSPTRHHD ASRSLADHRS RHAESQYSSE
860 870 880 890 900
PDSELLMLPR AKRGRSAECL HMTSELQPSL DRARSASTNC LRPDTSLHSP
910 920 930 940 950
ERERGRWSPS LARRRPASPR IQIQHASPEN DRHSRKSERS SIQKQSRKGT
960 970 980 990 1000
ASDADRVLPP CLSRRGYAIP RATDQPVIRG KHTTRSRSSE HSSIRTLCSM
1010 1020 1030 1040 1050
HHLAPGGSAP PSPLLTRTHR QGSPTQSPPA DTSFGSRRGR QLPQVPVRSG
1060 1070 1080 1090 1100
SIEQASLVVE ERTRQMKMKV HRFKQTTGSG SSQELDHEQY SKYNIHKDQY
1110 1120 1130 1140 1150
RSCDNASAKS SDSDVSDVSA ISRASSTSRL SSTSFMSEQS ERPRGRISSF
1160 1170 1180 1190 1200
TPKMQGRRMG TSGRAIIKST SVSGEIYTLE HNDGSQSDTA VGTVGAGGKK
1210 1220 1230 1240 1250
RRSSLSAKVV AIVSRRSRST SQLSQTESGH KKLKSTIQRS TETGMAAEMR
1260 1270 1280 1290 1300
KMVRQPSRES TDGSINSYSS EGNLIFPGVR VGPDSQFSDF LDGLGPAQLV
1310 1320 1330 1340 1350
GRQTLATPAM GDIQIGMEDK KGQLEVEVIR ARSLTQKPGS KSTPAPYVKV
1360 1370 1380 1390 1400
YLLENGACIA KKKTRIARKT LDPLYQQSLV FDESPQGKVL QVIVWGDYGR
1410 1420 1430 1440 1450
MDHKCFMGVA QILLEELDLS SMVIGWYKLF PPSSLVDPTL TPLTRRASQS
1460
SLESSSGPPC IRS
Length:1,463
Mass (Da):163,161
Last modified:January 9, 2007 - v2
Checksum:i1E0BE5D522924738
GO
Isoform 2 (identifier: Q99NE5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     373-388: GVSTPEYTSCEDVELE → YQTRYRSDPNLARYPV
     389-1463: Missing.

Show »
Length:388
Mass (Da):43,845
Checksum:iD928680A7C9516D9
GO
Isoform 3 (identifier: Q99NE5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     904-931: Missing.

Note: No experimental confirmation available.
Show »
Length:1,435
Mass (Da):159,940
Checksum:i1FC9923B9DB475D0
GO
Isoform 4 (identifier: Q99NE5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     904-931: Missing.
     956-1016: Missing.

Note: No experimental confirmation available.
Show »
Length:1,374
Mass (Da):153,345
Checksum:i6C99725DAB7FFE0D
GO
Isoform 5 (identifier: Q99NE5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     904-1016: Missing.

Note: No experimental confirmation available.
Show »
Length:1,350
Mass (Da):150,647
Checksum:i1DAECF1761B05766
GO
Isoform 6 (identifier: Q99NE5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     853-1016: Missing.

Note: No experimental confirmation available.
Show »
Length:1,299
Mass (Da):144,930
Checksum:i0D0D326E71A9C39A
GO
Isoform 7 (identifier: Q99NE5-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     369-369: K → KEEEYQTRYR...KRRQMSVSSS
     853-1016: Missing.
     1150-1170: FTPKMQGRRMGTSGRAIIKST → LTLVLLMMALPYKFVQKSRLF
     1171-1463: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:1,184
Mass (Da):132,994
Checksum:i40271B0A3D3ACD08
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022230369K → KEEEYQTRYRSDPNLARYPV KAPLEEQQMRMHARVSRARH ERRHSDVALPHTEAAAAVSA ETTAGKRAQTTARVSPPESP RARAPAAQPPAEHGPPPPRP APGPAEPPEPRVPEPLRKQG RLDPGSAVLLRKAKREKAES MLRNDSLSSDQSESVRPSPP KPHRPKRGGKRRQMSVSSS in isoform 7. 1 Publication1
Alternative sequenceiVSP_022231373 – 388GVSTP…DVELE → YQTRYRSDPNLARYPV in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_022232389 – 1463Missing in isoform 2. 1 PublicationAdd BLAST1075
Alternative sequenceiVSP_022233853 – 1016Missing in isoform 6 and isoform 7. 2 PublicationsAdd BLAST164
Alternative sequenceiVSP_022234904 – 1016Missing in isoform 5. 1 PublicationAdd BLAST113
Alternative sequenceiVSP_022235904 – 931Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_022236956 – 1016Missing in isoform 4. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_0222371150 – 1170FTPKM…IIKST → LTLVLLMMALPYKFVQKSRL F in isoform 7. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_0222381171 – 1463Missing in isoform 7. 1 PublicationAdd BLAST293

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ310531 mRNA. Translation: CAC32041.1.
AY356534 mRNA. Translation: AAR14797.1.
AY356535 mRNA. Translation: AAR14798.1.
AY356536 mRNA. Translation: AAR14799.1.
AY356537 mRNA. Translation: AAR14800.1.
AY356538 mRNA. Translation: AAR14801.1.
AK220335 mRNA. Translation: BAD90402.1.
RefSeqiNP_001012641.1. NM_001012623.1. [Q99NE5-4]
NP_001012642.1. NM_001012624.1. [Q99NE5-5]
NP_001012643.1. NM_001012625.1. [Q99NE5-6]
NP_444500.1. NM_053270.1. [Q99NE5-1]
NP_898839.2. NM_183018.2. [Q99NE5-3]
UniGeneiMm.380549.
Mm.416993.
Mm.461684.
Mm.60061.

Genome annotation databases

GeneIDi116837.
KEGGimmu:116837.
UCSCiuc029qni.1. mouse. [Q99NE5-1]
uc029qnj.1. mouse. [Q99NE5-3]
uc029qnk.1. mouse. [Q99NE5-4]
uc029qnm.1. mouse. [Q99NE5-6]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ310531 mRNA. Translation: CAC32041.1.
AY356534 mRNA. Translation: AAR14797.1.
AY356535 mRNA. Translation: AAR14798.1.
AY356536 mRNA. Translation: AAR14799.1.
AY356537 mRNA. Translation: AAR14800.1.
AY356538 mRNA. Translation: AAR14801.1.
AK220335 mRNA. Translation: BAD90402.1.
RefSeqiNP_001012641.1. NM_001012623.1. [Q99NE5-4]
NP_001012642.1. NM_001012624.1. [Q99NE5-5]
NP_001012643.1. NM_001012625.1. [Q99NE5-6]
NP_444500.1. NM_053270.1. [Q99NE5-1]
NP_898839.2. NM_183018.2. [Q99NE5-3]
UniGeneiMm.380549.
Mm.416993.
Mm.461684.
Mm.60061.

3D structure databases

ProteinModelPortaliQ99NE5.
SMRiQ99NE5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228025. 9 interactors.
IntActiQ99NE5. 2 interactors.
MINTiMINT-1210408.

PTM databases

iPTMnetiQ99NE5.
PhosphoSitePlusiQ99NE5.

Proteomic databases

PeptideAtlasiQ99NE5.
PRIDEiQ99NE5.

Protocols and materials databases

DNASUi116837.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116837.
KEGGimmu:116837.
UCSCiuc029qni.1. mouse. [Q99NE5-1]
uc029qnj.1. mouse. [Q99NE5-3]
uc029qnk.1. mouse. [Q99NE5-4]
uc029qnm.1. mouse. [Q99NE5-6]

Organism-specific databases

CTDi22999.
MGIiMGI:2152971. Rims1.
RougeiSearch...

Phylogenomic databases

HOVERGENiHBG058147.
InParanoidiQ99NE5.
KOiK15291.
PhylomeDBiQ99NE5.

Miscellaneous databases

ChiTaRSiRims1. mouse.
PROiQ99NE5.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RIMS1.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.40.150. 2 hits.
3.30.40.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR001478. PDZ.
IPR010911. Rab_BD.
IPR030630. RIM1.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR12157:SF19. PTHR12157:SF19. 2 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEiPS50004. C2. 2 hits.
PS50106. PDZ. 1 hit.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIMS1_MOUSE
AccessioniPrimary (citable) accession number: Q99NE5
Secondary accession number(s): Q5DU35
, Q5J8K0, Q5J8K1, Q5J8K2, Q5J8K3, Q5J8K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.