Reviewed, UniProtKB/Swiss-Prot Q99NE5 (RIMS1_MOUSE)

Names and origin

Protein names

Recommended name:
    Regulating synaptic membrane exocytosis protein 1
Alternative name(s):
    Rab3-interacting molecule 1
      Short name=RIM 1
    Rab3-interacting protein 1

Gene names

Name:	Rims1
Synonyms:	Kiaa0340, Rab3ip1, Rim1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Rab effector involved in exocytosis. May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity.
Subunit structure	Binds SNAP25, SYT1 and CACNA1B. Interaction with SYT1 is enhanced by calcium ions. Interaction with SNAP25 is weaker in the presence of calcium ions. Binds RIM binding proteins 1 and 2; interacts with PPFIA3 and PPFIA4 By similarity. Binds RAB3A, RAB3B and RAB3D that have been activated by GTP-binding. Binds UNC13A.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity. Cell junction › synapse By similarity. Cell junction › synapse › presynaptic cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Contains 2 C2 domains. Contains 1 FYVE-type zinc finger. Contains 1 PDZ (DHR) domain. Contains 1 RabBD (Rab-binding) domain.

Ontologies

Keywords
Biological process	Exocytosis Neurotransmitter transport Transport
Cellular component	Cell junction Cell membrane Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	exocytosis Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Inferred from electronic annotation. Source: InterPro neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of long-term neuronal synaptic plasticity Inferred from mutant phenotype. Source: MGI
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell presynaptic membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	Rab GTPase binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Fbxl20	Q9CZV8	2	EBI-775541,EBI-1551033

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Note: A number of isoforms are produced.

Isoform 1 (identifier: Q99NE5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99NE5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     373-388: GVSTPEYTSCEDVELE → YQTRYRSDPNLARYPV
     389-1463: Missing.

Isoform 3 (identifier: Q99NE5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     904-931: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q99NE5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     904-931: Missing.
     956-1016: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q99NE5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     904-1016: Missing.

Note: No experimental confirmation available.

Isoform 6 (identifier: Q99NE5-6)

The sequence of this isoform differs from the canonical sequence as follows:
     853-1016: Missing.

Note: No experimental confirmation available.

Isoform 7 (identifier: Q99NE5-7)

The sequence of this isoform differs from the canonical sequence as follows:
     369-369: K → KEEEYQTRYR...KRRQMSVSSS
     853-1016: Missing.
     1150-1170: FTPKMQGRRMGTSGRAIIKST → LTLVLLMMALPYKFVQKSRLF
     1171-1463: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1463	1463	Regulating synaptic membrane exocytosis protein 1		PRO_0000190199
Regions
	Domain	22 – 205	184	RabBD
	Domain	440 – 526	87	PDZ
	Domain	579 – 685	107	C2 1
	Domain	1309 – 1411	103	C2 2
	Zinc finger	133 – 193	61	FYVE-type
Amino acid modifications
	Modified residue	346	1	Phosphoserine Ref.5
	Modified residue	716	1	Phosphoserine Ref.6
	Modified residue	812	1	Phosphoserine Ref.5 Ref.6
	Modified residue	1023	1	Phosphoserine Ref.6
	Modified residue	1025	1	Phosphothreonine Ref.5 Ref.6
	Modified residue	1027	1	Phosphoserine Ref.5
	Modified residue	1441	1	Phosphothreonine By similarity
	Modified residue	1448	1	Phosphoserine Ref.6
	Modified residue	1450	1	Phosphoserine By similarity
	Modified residue	1451	1	Phosphoserine Ref.5
	Modified residue	1454	1	Phosphoserine By similarity
	Modified residue	1463	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	369	1	K → KEEEYQTRYRSDPNLARYPV KAPLEEQQMRMHARVSRARH ERRHSDVALPHTEAAAAVSA ETTAGKRAQTTARVSPPESP RARAPAAQPPAEHGPPPPRP APGPAEPPEPRVPEPLRKQG RLDPGSAVLLRKAKREKAES MLRNDSLSSDQSESVRPSPP KPHRPKRGGKRRQMSVSSS in isoform 7.		VSP_022230
	Alternative sequence	373 – 388	16	GVSTP…DVELE → YQTRYRSDPNLARYPV in isoform 2.		VSP_022231
	Alternative sequence	389 – 1463	1075	Missing in isoform 2.		VSP_022232
	Alternative sequence	853 – 1016	164	Missing in isoform 6 and isoform 7.		VSP_022233
	Alternative sequence	904 – 1016	113	Missing in isoform 5.		VSP_022234
	Alternative sequence	904 – 931	28	Missing in isoform 3 and isoform 4.		VSP_022235
	Alternative sequence	956 – 1016	61	Missing in isoform 4.		VSP_022236
	Alternative sequence	1150 – 1170	21	FTPKM…IIKST → LTLVLLMMALPYKFVQKSRL F in isoform 7.		VSP_022237
	Alternative sequence	1171 – 1463	293	Missing in isoform 7.		VSP_022238
Experimental info
	Mutagenesis	33	1	R → G: Abolishes interaction with RAB3A. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 1E0BE5D522924738	FASTA	1,463	163,161
	10 20 30 40 50 60 MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD 70 80 90 100 110 120 MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK 130 140 150 160 170 180 IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV 190 200 210 220 230 240 VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR 250 260 270 280 290 300 SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS 310 320 330 340 350 360 EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR 370 380 390 400 410 420 VAEDEKQRKE EEGVSTPEYT SCEDVELESE SVSEKGDLDY WLDPATWHSR ETSPISSHPV 430 440 450 460 470 480 TWQPSKEGDR LIGRVILNKR TTMPKESGAL LGLKVVGGKM TDLGRLGAFI TKVKKGSLAD 490 500 510 520 530 540 VVGHLRAGDE VLEWNGKPLP GATNEEVYNI ILESKSEPQV EIIVSRPIGD IPRIPESSHP 550 560 570 580 590 600 PLESSSSSFE SQKMERPSIS VISPTSPGAL KDAPQVLPGQ LSVKLWYDKV GHQLIVNVLQ 610 620 630 640 650 660 ATDLPPRVDG RPRNPYVKMY FLPDRSDKSK RRTKTVKKLL EPKWNQTFVY SHVHRRDFRE 670 680 690 700 710 720 RMLEITVWDQ PRVQDEESEF LGEILIELET ALLDDEPHWY KLQTHDESSL PLPQPSPFMP 730 740 750 760 770 780 RRHIHGESSS KKLQRSQRIS DSDISDYEVD DGIGVVPPVG YRASARESKA TTLTVPEQQR 790 800 810 820 830 840 TTHHRSRSVS PHRGDDQGRP RSRLPNVPLQ RSLDEIHPTR RSRSPTRHHD ASRSLADHRS 850 860 870 880 890 900 RHAESQYSSE PDSELLMLPR AKRGRSAECL HMTSELQPSL DRARSASTNC LRPDTSLHSP 910 920 930 940 950 960 ERERGRWSPS LARRRPASPR IQIQHASPEN DRHSRKSERS SIQKQSRKGT ASDADRVLPP 970 980 990 1000 1010 1020 CLSRRGYAIP RATDQPVIRG KHTTRSRSSE HSSIRTLCSM HHLAPGGSAP PSPLLTRTHR 1030 1040 1050 1060 1070 1080 QGSPTQSPPA DTSFGSRRGR QLPQVPVRSG SIEQASLVVE ERTRQMKMKV HRFKQTTGSG 1090 1100 1110 1120 1130 1140 SSQELDHEQY SKYNIHKDQY RSCDNASAKS SDSDVSDVSA ISRASSTSRL SSTSFMSEQS 1150 1160 1170 1180 1190 1200 ERPRGRISSF TPKMQGRRMG TSGRAIIKST SVSGEIYTLE HNDGSQSDTA VGTVGAGGKK 1210 1220 1230 1240 1250 1260 RRSSLSAKVV AIVSRRSRST SQLSQTESGH KKLKSTIQRS TETGMAAEMR KMVRQPSRES 1270 1280 1290 1300 1310 1320 TDGSINSYSS EGNLIFPGVR VGPDSQFSDF LDGLGPAQLV GRQTLATPAM GDIQIGMEDK 1330 1340 1350 1360 1370 1380 KGQLEVEVIR ARSLTQKPGS KSTPAPYVKV YLLENGACIA KKKTRIARKT LDPLYQQSLV 1390 1400 1410 1420 1430 1440 FDESPQGKVL QVIVWGDYGR MDHKCFMGVA QILLEELDLS SMVIGWYKLF PPSSLVDPTL 1450 1460 TPLTRRASQS SLESSSGPPC IRS
	Isoform 2. Checksum: D928680A7C9516D9	FASTA	388	43,845
	10 20 30 40 50 60 MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD 70 80 90 100 110 120 MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK 130 140 150 160 170 180 IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV 190 200 210 220 230 240 VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR 250 260 270 280 290 300 SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS 310 320 330 340 350 360 EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR 370 380 VAEDEKQRKE EEYQTRYRSD PNLARYPV
	Isoform 3. Checksum: 1FC9923B9DB475D0	FASTA	1,435	159,940
	10 20 30 40 50 60 MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD 70 80 90 100 110 120 MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK 130 140 150 160 170 180 IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV 190 200 210 220 230 240 VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR 250 260 270 280 290 300 SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS 310 320 330 340 350 360 EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR 370 380 390 400 410 420 VAEDEKQRKE EEGVSTPEYT SCEDVELESE SVSEKGDLDY WLDPATWHSR ETSPISSHPV 430 440 450 460 470 480 TWQPSKEGDR LIGRVILNKR TTMPKESGAL LGLKVVGGKM TDLGRLGAFI TKVKKGSLAD 490 500 510 520 530 540 VVGHLRAGDE VLEWNGKPLP GATNEEVYNI ILESKSEPQV EIIVSRPIGD IPRIPESSHP 550 560 570 580 590 600 PLESSSSSFE SQKMERPSIS VISPTSPGAL KDAPQVLPGQ LSVKLWYDKV GHQLIVNVLQ 610 620 630 640 650 660 ATDLPPRVDG RPRNPYVKMY FLPDRSDKSK RRTKTVKKLL EPKWNQTFVY SHVHRRDFRE 670 680 690 700 710 720 RMLEITVWDQ PRVQDEESEF LGEILIELET ALLDDEPHWY KLQTHDESSL PLPQPSPFMP 730 740 750 760 770 780 RRHIHGESSS KKLQRSQRIS DSDISDYEVD DGIGVVPPVG YRASARESKA TTLTVPEQQR 790 800 810 820 830 840 TTHHRSRSVS PHRGDDQGRP RSRLPNVPLQ RSLDEIHPTR RSRSPTRHHD ASRSLADHRS 850 860 870 880 890 900 RHAESQYSSE PDSELLMLPR AKRGRSAECL HMTSELQPSL DRARSASTNC LRPDTSLHSP 910 920 930 940 950 960 ERERHSRKSE RSSIQKQSRK GTASDADRVL PPCLSRRGYA IPRATDQPVI RGKHTTRSRS 970 980 990 1000 1010 1020 SEHSSIRTLC SMHHLAPGGS APPSPLLTRT HRQGSPTQSP PADTSFGSRR GRQLPQVPVR 1030 1040 1050 1060 1070 1080 SGSIEQASLV VEERTRQMKM KVHRFKQTTG SGSSQELDHE QYSKYNIHKD QYRSCDNASA 1090 1100 1110 1120 1130 1140 KSSDSDVSDV SAISRASSTS RLSSTSFMSE QSERPRGRIS SFTPKMQGRR MGTSGRAIIK 1150 1160 1170 1180 1190 1200 STSVSGEIYT LEHNDGSQSD TAVGTVGAGG KKRRSSLSAK VVAIVSRRSR STSQLSQTES 1210 1220 1230 1240 1250 1260 GHKKLKSTIQ RSTETGMAAE MRKMVRQPSR ESTDGSINSY SSEGNLIFPG VRVGPDSQFS 1270 1280 1290 1300 1310 1320 DFLDGLGPAQ LVGRQTLATP AMGDIQIGME DKKGQLEVEV IRARSLTQKP GSKSTPAPYV 1330 1340 1350 1360 1370 1380 KVYLLENGAC IAKKKTRIAR KTLDPLYQQS LVFDESPQGK VLQVIVWGDY GRMDHKCFMG 1390 1400 1410 1420 1430 VAQILLEELD LSSMVIGWYK LFPPSSLVDP TLTPLTRRAS QSSLESSSGP PCIRS
	Isoform 4. Checksum: 6C99725DAB7FFE0D	FASTA	1,374	153,345
	10 20 30 40 50 60 MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD 70 80 90 100 110 120 MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK 130 140 150 160 170 180 IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV 190 200 210 220 230 240 VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR 250 260 270 280 290 300 SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS 310 320 330 340 350 360 EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR 370 380 390 400 410 420 VAEDEKQRKE EEGVSTPEYT SCEDVELESE SVSEKGDLDY WLDPATWHSR ETSPISSHPV 430 440 450 460 470 480 TWQPSKEGDR LIGRVILNKR TTMPKESGAL LGLKVVGGKM TDLGRLGAFI TKVKKGSLAD 490 500 510 520 530 540 VVGHLRAGDE VLEWNGKPLP GATNEEVYNI ILESKSEPQV EIIVSRPIGD IPRIPESSHP 550 560 570 580 590 600 PLESSSSSFE SQKMERPSIS VISPTSPGAL KDAPQVLPGQ LSVKLWYDKV GHQLIVNVLQ 610 620 630 640 650 660 ATDLPPRVDG RPRNPYVKMY FLPDRSDKSK RRTKTVKKLL EPKWNQTFVY SHVHRRDFRE 670 680 690 700 710 720 RMLEITVWDQ PRVQDEESEF LGEILIELET ALLDDEPHWY KLQTHDESSL PLPQPSPFMP 730 740 750 760 770 780 RRHIHGESSS KKLQRSQRIS DSDISDYEVD DGIGVVPPVG YRASARESKA TTLTVPEQQR 790 800 810 820 830 840 TTHHRSRSVS PHRGDDQGRP RSRLPNVPLQ RSLDEIHPTR RSRSPTRHHD ASRSLADHRS 850 860 870 880 890 900 RHAESQYSSE PDSELLMLPR AKRGRSAECL HMTSELQPSL DRARSASTNC LRPDTSLHSP 910 920 930 940 950 960 ERERHSRKSE RSSIQKQSRK GTASDADRTH RQGSPTQSPP ADTSFGSRRG RQLPQVPVRS 970 980 990 1000 1010 1020 GSIEQASLVV EERTRQMKMK VHRFKQTTGS GSSQELDHEQ YSKYNIHKDQ YRSCDNASAK 1030 1040 1050 1060 1070 1080 SSDSDVSDVS AISRASSTSR LSSTSFMSEQ SERPRGRISS FTPKMQGRRM GTSGRAIIKS 1090 1100 1110 1120 1130 1140 TSVSGEIYTL EHNDGSQSDT AVGTVGAGGK KRRSSLSAKV VAIVSRRSRS TSQLSQTESG 1150 1160 1170 1180 1190 1200 HKKLKSTIQR STETGMAAEM RKMVRQPSRE STDGSINSYS SEGNLIFPGV RVGPDSQFSD 1210 1220 1230 1240 1250 1260 FLDGLGPAQL VGRQTLATPA MGDIQIGMED KKGQLEVEVI RARSLTQKPG SKSTPAPYVK 1270 1280 1290 1300 1310 1320 VYLLENGACI AKKKTRIARK TLDPLYQQSL VFDESPQGKV LQVIVWGDYG RMDHKCFMGV 1330 1340 1350 1360 1370 AQILLEELDL SSMVIGWYKL FPPSSLVDPT LTPLTRRASQ SSLESSSGPP CIRS
	Isoform 5. Checksum: 1DAECF1761B05766	FASTA	1,350	150,647
	10 20 30 40 50 60 MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD 70 80 90 100 110 120 MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK 130 140 150 160 170 180 IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV 190 200 210 220 230 240 VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR 250 260 270 280 290 300 SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS 310 320 330 340 350 360 EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR 370 380 390 400 410 420 VAEDEKQRKE EEGVSTPEYT SCEDVELESE SVSEKGDLDY WLDPATWHSR ETSPISSHPV 430 440 450 460 470 480 TWQPSKEGDR LIGRVILNKR TTMPKESGAL LGLKVVGGKM TDLGRLGAFI TKVKKGSLAD 490 500 510 520 530 540 VVGHLRAGDE VLEWNGKPLP GATNEEVYNI ILESKSEPQV EIIVSRPIGD IPRIPESSHP 550 560 570 580 590 600 PLESSSSSFE SQKMERPSIS VISPTSPGAL KDAPQVLPGQ LSVKLWYDKV GHQLIVNVLQ 610 620 630 640 650 660 ATDLPPRVDG RPRNPYVKMY FLPDRSDKSK RRTKTVKKLL EPKWNQTFVY SHVHRRDFRE 670 680 690 700 710 720 RMLEITVWDQ PRVQDEESEF LGEILIELET ALLDDEPHWY KLQTHDESSL PLPQPSPFMP 730 740 750 760 770 780 RRHIHGESSS KKLQRSQRIS DSDISDYEVD DGIGVVPPVG YRASARESKA TTLTVPEQQR 790 800 810 820 830 840 TTHHRSRSVS PHRGDDQGRP RSRLPNVPLQ RSLDEIHPTR RSRSPTRHHD ASRSLADHRS 850 860 870 880 890 900 RHAESQYSSE PDSELLMLPR AKRGRSAECL HMTSELQPSL DRARSASTNC LRPDTSLHSP 910 920 930 940 950 960 ERERTHRQGS PTQSPPADTS FGSRRGRQLP QVPVRSGSIE QASLVVEERT RQMKMKVHRF 970 980 990 1000 1010 1020 KQTTGSGSSQ ELDHEQYSKY NIHKDQYRSC DNASAKSSDS DVSDVSAISR ASSTSRLSST 1030 1040 1050 1060 1070 1080 SFMSEQSERP RGRISSFTPK MQGRRMGTSG RAIIKSTSVS GEIYTLEHND GSQSDTAVGT 1090 1100 1110 1120 1130 1140 VGAGGKKRRS SLSAKVVAIV SRRSRSTSQL SQTESGHKKL KSTIQRSTET GMAAEMRKMV 1150 1160 1170 1180 1190 1200 RQPSRESTDG SINSYSSEGN LIFPGVRVGP DSQFSDFLDG LGPAQLVGRQ TLATPAMGDI 1210 1220 1230 1240 1250 1260 QIGMEDKKGQ LEVEVIRARS LTQKPGSKST PAPYVKVYLL ENGACIAKKK TRIARKTLDP 1270 1280 1290 1300 1310 1320 LYQQSLVFDE SPQGKVLQVI VWGDYGRMDH KCFMGVAQIL LEELDLSSMV IGWYKLFPPS 1330 1340 1350 SLVDPTLTPL TRRASQSSLE SSSGPPCIRS
	Isoform 6. Checksum: 0D0D326E71A9C39A	FASTA	1,299	144,930
	10 20 30 40 50 60 MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD 70 80 90 100 110 120 MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK 130 140 150 160 170 180 IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV 190 200 210 220 230 240 VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR 250 260 270 280 290 300 SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS 310 320 330 340 350 360 EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR 370 380 390 400 410 420 VAEDEKQRKE EEGVSTPEYT SCEDVELESE SVSEKGDLDY WLDPATWHSR ETSPISSHPV 430 440 450 460 470 480 TWQPSKEGDR LIGRVILNKR TTMPKESGAL LGLKVVGGKM TDLGRLGAFI TKVKKGSLAD 490 500 510 520 530 540 VVGHLRAGDE VLEWNGKPLP GATNEEVYNI ILESKSEPQV EIIVSRPIGD IPRIPESSHP 550 560 570 580 590 600 PLESSSSSFE SQKMERPSIS VISPTSPGAL KDAPQVLPGQ LSVKLWYDKV GHQLIVNVLQ 610 620 630 640 650 660 ATDLPPRVDG RPRNPYVKMY FLPDRSDKSK RRTKTVKKLL EPKWNQTFVY SHVHRRDFRE 670 680 690 700 710 720 RMLEITVWDQ PRVQDEESEF LGEILIELET ALLDDEPHWY KLQTHDESSL PLPQPSPFMP 730 740 750 760 770 780 RRHIHGESSS KKLQRSQRIS DSDISDYEVD DGIGVVPPVG YRASARESKA TTLTVPEQQR 790 800 810 820 830 840 TTHHRSRSVS PHRGDDQGRP RSRLPNVPLQ RSLDEIHPTR RSRSPTRHHD ASRSLADHRS 850 860 870 880 890 900 RHAESQYSSE PDRTHRQGSP TQSPPADTSF GSRRGRQLPQ VPVRSGSIEQ ASLVVEERTR 910 920 930 940 950 960 QMKMKVHRFK QTTGSGSSQE LDHEQYSKYN IHKDQYRSCD NASAKSSDSD VSDVSAISRA 970 980 990 1000 1010 1020 SSTSRLSSTS FMSEQSERPR GRISSFTPKM QGRRMGTSGR AIIKSTSVSG EIYTLEHNDG 1030 1040 1050 1060 1070 1080 SQSDTAVGTV GAGGKKRRSS LSAKVVAIVS RRSRSTSQLS QTESGHKKLK STIQRSTETG 1090 1100 1110 1120 1130 1140 MAAEMRKMVR QPSRESTDGS INSYSSEGNL IFPGVRVGPD SQFSDFLDGL GPAQLVGRQT 1150 1160 1170 1180 1190 1200 LATPAMGDIQ IGMEDKKGQL EVEVIRARSL TQKPGSKSTP APYVKVYLLE NGACIAKKKT 1210 1220 1230 1240 1250 1260 RIARKTLDPL YQQSLVFDES PQGKVLQVIV WGDYGRMDHK CFMGVAQILL EELDLSSMVI 1270 1280 1290 GWYKLFPPSS LVDPTLTPLT RRASQSSLES SSGPPCIRS
	Isoform 7. Checksum: 40271B0A3D3ACD08	FASTA	1,184	132,994
	10 20 30 40 50 60 MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD 70 80 90 100 110 120 MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK 130 140 150 160 170 180 IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV 190 200 210 220 230 240 VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR 250 260 270 280 290 300 SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS 310 320 330 340 350 360 EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR 370 380 390 400 410 420 VAEDEKQRKE EEYQTRYRSD PNLARYPVKA PLEEQQMRMH ARVSRARHER RHSDVALPHT 430 440 450 460 470 480 EAAAAVSAET TAGKRAQTTA RVSPPESPRA RAPAAQPPAE HGPPPPRPAP GPAEPPEPRV 490 500 510 520 530 540 PEPLRKQGRL DPGSAVLLRK AKREKAESML RNDSLSSDQS ESVRPSPPKP HRPKRGGKRR 550 560 570 580 590 600 QMSVSSSEEE GVSTPEYTSC EDVELESESV SEKGDLDYWL DPATWHSRET SPISSHPVTW 610 620 630 640 650 660 QPSKEGDRLI GRVILNKRTT MPKESGALLG LKVVGGKMTD LGRLGAFITK VKKGSLADVV 670 680 690 700 710 720 GHLRAGDEVL EWNGKPLPGA TNEEVYNIIL ESKSEPQVEI IVSRPIGDIP RIPESSHPPL 730 740 750 760 770 780 ESSSSSFESQ KMERPSISVI SPTSPGALKD APQVLPGQLS VKLWYDKVGH QLIVNVLQAT 790 800 810 820 830 840 DLPPRVDGRP RNPYVKMYFL PDRSDKSKRR TKTVKKLLEP KWNQTFVYSH VHRRDFRERM 850 860 870 880 890 900 LEITVWDQPR VQDEESEFLG EILIELETAL LDDEPHWYKL QTHDESSLPL PQPSPFMPRR 910 920 930 940 950 960 HIHGESSSKK LQRSQRISDS DISDYEVDDG IGVVPPVGYR ASARESKATT LTVPEQQRTT 970 980 990 1000 1010 1020 HHRSRSVSPH RGDDQGRPRS RLPNVPLQRS LDEIHPTRRS RSPTRHHDAS RSLADHRSRH 1030 1040 1050 1060 1070 1080 AESQYSSEPD RTHRQGSPTQ SPPADTSFGS RRGRQLPQVP VRSGSIEQAS LVVEERTRQM 1090 1100 1110 1120 1130 1140 KMKVHRFKQT TGSGSSQELD HEQYSKYNIH KDQYRSCDNA SAKSSDSDVS DVSAISRASS 1150 1160 1170 1180 TSRLSSTSFM SEQSERPRGR ISSLTLVLLM MALPYKFVQK SRLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha-helix motifs." Wang X., Hu B., Zimmermann B., Kilimann M.W. J. Biol. Chem. 276:32480-32488(2001) [PubMed: 11431472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING, MUTAGENESIS OF ARG-33, INTERACTION WITH RAB3A; RAB3B AND RAB3D. Tissue: Brain.
[2]	Wang X., Hu B., Kilimann M.W. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3; 4; 5 AND 6).
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 202-1463 (ISOFORM 7). Tissue: Brain.
[4]	"RIM1alpha forms a protein scaffold for regulating neurotransmitter release at the active zone." Schoch S., Castillo P.E., Jo T., Mukherjee K., Geppert M., Wang Y., Schmitz F., Malenka R.C., Suedhof T.C. Nature 415:321-326(2002) [PubMed: 11797009] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-346; SER-812; THR-1025; SER-1027 AND SER-1451, MASS SPECTROMETRY. Tissue: Brain.
[6]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716; SER-812; SER-1023; THR-1025 AND SER-1448, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ310531 mRNA. Translation: CAC32041.1. AY356534 mRNA. Translation: AAR14797.1. AY356535 mRNA. Translation: AAR14798.1. AY356536 mRNA. Translation: AAR14799.1. AY356537 mRNA. Translation: AAR14800.1. AY356538 mRNA. Translation: AAR14801.1. AK220335 mRNA. Translation: BAD90402.1.
IPI	IPI00118692. IPI00136021. IPI00553549. IPI00553696. IPI00553828. IPI00816873. IPI00816908.
RefSeq	NP_001012641.1. NP_001012642.1. NP_001012643.1. NP_444500.1. NP_898839.2.
UniGene	Mm.380549 Mm.461684 Mm.60061
3D structure databases
SMR	Q99NE5. Positions 136-193, 418-526, 575-703.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99NE5. 2 interactions.
STRING	Q99NE5.
Proteomic databases
PRIDE	Q99NE5.
Genome annotation databases
GeneID	116837.
KEGG	mmu:116837.
Organism-specific databases
CTD	116837.
MGI	MGI:2152971. Rims1.
Rouge	Search...
Phylogenomic databases
HOVERGEN	Q99NE5.
Gene expression databases
CleanEx	MM_RIMS1.
Genevestigator	Q99NE5.
GermOnline	ENSMUSG00000041670. Mus musculus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_reg_CaLB. IPR018029. C2_membr_targeting. IPR001478. PDZ/DHR/GLGF. IPR010911. Rab_bd_domain. IPR017455. Znf_FYVE-rel. IPR011011. Znf_FYVE_PHD. [Graphical view]
Pfam	PF00168. C2. 2 hits. PF00595. PDZ. 1 hit. [Graphical view]
SMART	SM00239. C2. 2 hits. SM00228. PDZ. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 2 hits. PS50106. PDZ. 1 hit. PS50916. RABBD. 1 hit. PS50178. ZF_FYVE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	369178.
SOURCE	Search...

Entry information

Entry name

RIMS1_MOUSE

Accession

Primary (citable) accession number: Q99NE5
Secondary accession number(s): Q5DU35 , Q5J8K0, Q5J8K1, Q5J8K2, Q5J8K3, Q5J8K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	January 9, 2007
Last modified:	November 24, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)