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Protein

Acyl-coenzyme A thioesterase 12

Gene

Acot12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes acetyl-CoA to acetate and CoA.

Catalytic activityi

Acetyl-CoA + H2O = CoA + acetate.

Enzyme regulationi

Allosterically regulated by ATP (activator) and ADP (inhibitor).

Pathwayi: pyruvate metabolism

This protein is involved in the pathway pyruvate metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway pyruvate metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145Coenzyme ABy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.2.1. 5301.
SABIO-RKQ99NB7.
UniPathwayiUPA00231.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 12 (EC:3.1.2.1)
Short name:
Acyl-CoA thioesterase 12
Alternative name(s):
Acyl-CoA thioester hydrolase 12
Cytoplasmic acetyl-CoA hydrolase 1
Short name:
CACH-1
Short name:
rACH
Short name:
rCACH-1
Gene namesi
Name:Acot12
Synonyms:Cach, Cach1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619752. Acot12.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538111 – 556Acyl-coenzyme A thioesterase 12Add BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34N6-succinyllysineBy similarity1
Modified residuei97N6-succinyllysineBy similarity1
Modified residuei160N6-succinyllysineBy similarity1
Modified residuei229N6-succinyllysineBy similarity1

Proteomic databases

PaxDbiQ99NB7.
PRIDEiQ99NB7.

Expressioni

Inductioni

By 2-(p-chlorophenoxy)isobutyric acid (CPIB).

Interactioni

Subunit structurei

Active homodimer or homotetramer at room temperature and inactive monomer at low temperature.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021675.

Structurei

3D structure databases

ProteinModelPortaliQ99NB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 118HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST113
Domaini180 – 295HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST116
Domaini341 – 550STARTPROSITE-ProRule annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 56Coenzyme A bindingBy similarity3
Regioni83 – 85Coenzyme A bindingBy similarity3
Regioni235 – 237Coenzyme A bindingBy similarity3

Sequence similaritiesi

Contains 2 HotDog ACOT-type domains.PROSITE-ProRule annotation
Contains 1 START domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2763. Eukaryota.
COG1607. LUCA.
HOGENOMiHOG000232032.
HOVERGENiHBG023847.
InParanoidiQ99NB7.
KOiK01067.
PhylomeDBiQ99NB7.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.530.20. 1 hit.
InterProiIPR033120. HOTDOG_ACOT.
IPR029069. HotDog_dom.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 2 hits.
PF01852. START. 1 hit.
[Graphical view]
SMARTiSM00234. START. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 2 hits.
PROSITEiPS51770. HOTDOG_ACOT. 2 hits.
PS50848. START. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99NB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPTVAPGEV LMSQAIQPAH ADSRGELSAG QLLKWMDTTA CLAAEKHAGI
60 70 80 90 100
SCVTASMDDI LFEDTARIGQ IVTIRAKVTR AFSTSMEISI KVRVQDKFTG
110 120 130 140 150
IQKLLCVAFS TFVVKPLGKE KVHLKPVLLQ TEQEQVEHRL ASERRKVRLQ
160 170 180 190 200
HENTFSNIMK ESNWLRDPVC NEEEGTATTM ATSVQSIELV LPPHANHHGN
210 220 230 240 250
TFGGQIMAWM ETVATISASR LCHGHPFLKS VDMFKFRGPS TVGDRLVFNA
260 270 280 290 300
IVNNTFQNSV EVGVRVEAFD CREWAEGQGR HINSAFLIYN AVDDQEELIT
310 320 330 340 350
FPRIQPISKD DFRRYQGAIA RRRIRLGRKY VISHKKEVPL GTQWDISKKG
360 370 380 390 400
SISNTNVEAL KNLASKSGWE ITTTLEKIKI YTLEEQDAIS VKVEKQVGSP
410 420 430 440 450
ARVAYHLLSD FTKRPLWDPH YISCEVIDQV SEDDQIYYIT CSVVNGDKPK
460 470 480 490 500
DFVVLVSQRK PLKDDNTYIV ALMSVVLPSV PPSPQYIRSQ VICAGFLIQP
510 520 530 540 550
VDSSSCTVAY LNQMSDSILP YFAGNIGGWS KSIEEAAASC IKFIENATHD

GLKSVL
Length:556
Mass (Da):62,018
Last modified:June 1, 2001 - v1
Checksum:i81E592F066AB0C9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040609 mRNA. Translation: BAB39852.1.
RefSeqiNP_570103.1. NM_130747.1.
UniGeneiRn.212205.

Genome annotation databases

GeneIDi170570.
KEGGirno:170570.
UCSCiRGD:619752. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040609 mRNA. Translation: BAB39852.1.
RefSeqiNP_570103.1. NM_130747.1.
UniGeneiRn.212205.

3D structure databases

ProteinModelPortaliQ99NB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021675.

Proteomic databases

PaxDbiQ99NB7.
PRIDEiQ99NB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi170570.
KEGGirno:170570.
UCSCiRGD:619752. rat.

Organism-specific databases

CTDi134526.
RGDi619752. Acot12.

Phylogenomic databases

eggNOGiKOG2763. Eukaryota.
COG1607. LUCA.
HOGENOMiHOG000232032.
HOVERGENiHBG023847.
InParanoidiQ99NB7.
KOiK01067.
PhylomeDBiQ99NB7.

Enzyme and pathway databases

UniPathwayiUPA00231.
BRENDAi3.1.2.1. 5301.
SABIO-RKQ99NB7.

Miscellaneous databases

PROiQ99NB7.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.530.20. 1 hit.
InterProiIPR033120. HOTDOG_ACOT.
IPR029069. HotDog_dom.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
IPR006683. Thioestr_dom.
[Graphical view]
PfamiPF03061. 4HBT. 2 hits.
PF01852. START. 1 hit.
[Graphical view]
SMARTiSM00234. START. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 2 hits.
PROSITEiPS51770. HOTDOG_ACOT. 2 hits.
PS50848. START. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACO12_RAT
AccessioniPrimary (citable) accession number: Q99NB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: June 1, 2001
Last modified: October 5, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.