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Q99NB7

- ACO12_RAT

UniProt

Q99NB7 - ACO12_RAT

Protein

Acyl-coenzyme A thioesterase 12

Gene

Acot12

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Hydrolyzes acetyl-CoA to acetate and CoA.

    Catalytic activityi

    Acetyl-CoA + H2O = CoA + acetate.

    Enzyme regulationi

    Allosterically regulated by ATP (activator) and ADP (inhibitor).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451Coenzyme ABy similarity

    GO - Molecular functioni

    1. acetyl-CoA hydrolase activity Source: HGNC
    2. ATP binding Source: BHF-UCL
    3. lipid binding Source: InterPro

    GO - Biological processi

    1. acyl-CoA metabolic process Source: HGNC
    2. fatty acid metabolic process Source: UniProtKB-KW
    3. pyruvate metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi3.1.2.1. 5301.
    SABIO-RKQ99NB7.
    UniPathwayiUPA00231.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A thioesterase 12 (EC:3.1.2.1)
    Short name:
    Acyl-CoA thioesterase 12
    Alternative name(s):
    Acyl-CoA thioester hydrolase 12
    Cytoplasmic acetyl-CoA hydrolase 1
    Short name:
    CACH-1
    Short name:
    rACH
    Short name:
    rCACH-1
    Gene namesi
    Name:Acot12
    Synonyms:Cach, Cach1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619752. Acot12.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 556556Acyl-coenzyme A thioesterase 12PRO_0000053811Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei34 – 341N6-succinyllysineBy similarity
    Modified residuei97 – 971N6-succinyllysineBy similarity
    Modified residuei160 – 1601N6-succinyllysineBy similarity
    Modified residuei229 – 2291N6-succinyllysineBy similarity

    Proteomic databases

    PRIDEiQ99NB7.

    Expressioni

    Inductioni

    By 2-(p-chlorophenoxy)isobutyric acid (CPIB).

    Gene expression databases

    GenevestigatoriQ99NB7.

    Interactioni

    Subunit structurei

    Active homodimer or homotetramer at room temperature and inactive monomer at low temperature.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000021675.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99NB7.
    SMRiQ99NB7. Positions 8-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 128128Acyl coenzyme A hydrolase 1Add
    BLAST
    Domaini162 – 302141Acyl coenzyme A hydrolase 2Add
    BLAST
    Domaini341 – 550210STARTPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 563Coenzyme A bindingBy similarity
    Regioni83 – 853Coenzyme A bindingBy similarity
    Regioni235 – 2373Coenzyme A bindingBy similarity

    Sequence similaritiesi

    Contains 1 START domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1607.
    HOGENOMiHOG000232032.
    HOVERGENiHBG023847.
    InParanoidiQ99NB7.
    KOiK01067.
    PhylomeDBiQ99NB7.

    Family and domain databases

    Gene3Di3.10.129.10. 2 hits.
    3.30.530.20. 1 hit.
    InterProiIPR029069. HotDog_dom.
    IPR023393. START-like_dom.
    IPR002913. START_lipid-bd_dom.
    IPR006683. Thioestr_supf.
    [Graphical view]
    PfamiPF03061. 4HBT. 2 hits.
    PF01852. START. 1 hit.
    [Graphical view]
    SUPFAMiSSF54637. SSF54637. 2 hits.
    PROSITEiPS50848. START. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99NB7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPTVAPGEV LMSQAIQPAH ADSRGELSAG QLLKWMDTTA CLAAEKHAGI    50
    SCVTASMDDI LFEDTARIGQ IVTIRAKVTR AFSTSMEISI KVRVQDKFTG 100
    IQKLLCVAFS TFVVKPLGKE KVHLKPVLLQ TEQEQVEHRL ASERRKVRLQ 150
    HENTFSNIMK ESNWLRDPVC NEEEGTATTM ATSVQSIELV LPPHANHHGN 200
    TFGGQIMAWM ETVATISASR LCHGHPFLKS VDMFKFRGPS TVGDRLVFNA 250
    IVNNTFQNSV EVGVRVEAFD CREWAEGQGR HINSAFLIYN AVDDQEELIT 300
    FPRIQPISKD DFRRYQGAIA RRRIRLGRKY VISHKKEVPL GTQWDISKKG 350
    SISNTNVEAL KNLASKSGWE ITTTLEKIKI YTLEEQDAIS VKVEKQVGSP 400
    ARVAYHLLSD FTKRPLWDPH YISCEVIDQV SEDDQIYYIT CSVVNGDKPK 450
    DFVVLVSQRK PLKDDNTYIV ALMSVVLPSV PPSPQYIRSQ VICAGFLIQP 500
    VDSSSCTVAY LNQMSDSILP YFAGNIGGWS KSIEEAAASC IKFIENATHD 550
    GLKSVL 556
    Length:556
    Mass (Da):62,018
    Last modified:June 1, 2001 - v1
    Checksum:i81E592F066AB0C9E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB040609 mRNA. Translation: BAB39852.1.
    RefSeqiNP_570103.1. NM_130747.1.
    UniGeneiRn.212205.

    Genome annotation databases

    GeneIDi170570.
    KEGGirno:170570.
    UCSCiRGD:619752. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB040609 mRNA. Translation: BAB39852.1 .
    RefSeqi NP_570103.1. NM_130747.1.
    UniGenei Rn.212205.

    3D structure databases

    ProteinModelPortali Q99NB7.
    SMRi Q99NB7. Positions 8-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000021675.

    Proteomic databases

    PRIDEi Q99NB7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 170570.
    KEGGi rno:170570.
    UCSCi RGD:619752. rat.

    Organism-specific databases

    CTDi 134526.
    RGDi 619752. Acot12.

    Phylogenomic databases

    eggNOGi COG1607.
    HOGENOMi HOG000232032.
    HOVERGENi HBG023847.
    InParanoidi Q99NB7.
    KOi K01067.
    PhylomeDBi Q99NB7.

    Enzyme and pathway databases

    UniPathwayi UPA00231 .
    BRENDAi 3.1.2.1. 5301.
    SABIO-RK Q99NB7.

    Miscellaneous databases

    NextBioi 621056.

    Gene expression databases

    Genevestigatori Q99NB7.

    Family and domain databases

    Gene3Di 3.10.129.10. 2 hits.
    3.30.530.20. 1 hit.
    InterProi IPR029069. HotDog_dom.
    IPR023393. START-like_dom.
    IPR002913. START_lipid-bd_dom.
    IPR006683. Thioestr_supf.
    [Graphical view ]
    Pfami PF03061. 4HBT. 2 hits.
    PF01852. START. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54637. SSF54637. 2 hits.
    PROSITEi PS50848. START. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional expression of rat liver cytosolic acetyl-CoA hydrolase."
      Suematsu N., Okamoto K., Shibata K., Nakanishi Y., Isohashi F.
      Eur. J. Biochem. 268:2700-2709(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 161-174 AND 352-364.
      Strain: Donryu.
      Tissue: Liver.

    Entry informationi

    Entry nameiACO12_RAT
    AccessioniPrimary (citable) accession number: Q99NB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3