Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Gene

B3gnt5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc4Cer; paragloboside), resulting in the synthesis of Lc3Cer and neolactopentaosylceramide (nLc5Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • brain development Source: RGD
  • protein glycosylation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.206 5301
ReactomeiR-RNO-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase (EC:2.4.1.206)
Alternative name(s):
Lactotriaosylceramide synthase
Short name:
Lc(3)Cer synthase
Short name:
Lc3 synthase
UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
BGnT-5
Short name:
Beta-1,3-Gn-T5
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
Beta3Gn-T5
Gene namesi
Name:B3gnt5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi70955 B3gnt5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
Transmembranei15 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini36 – 377LumenalSequence analysisAdd BLAST342

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892121 – 377Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferaseAdd BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ99NB2
PRIDEiQ99NB2

Expressioni

Gene expression databases

BgeeiENSRNOG00000046258
GenevisibleiQ99NB2 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067153

Structurei

3D structure databases

ProteinModelPortaliQ99NB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287 Eukaryota
ENOG410ZZ1B LUCA
GeneTreeiENSGT00760000118879
HOGENOMiHOG000064519
HOVERGENiHBG101684
InParanoidiQ99NB2
KOiK03766
OMAiTWGNEKY
OrthoDBiEOG091G0AXM
PhylomeDBiQ99NB2
TreeFamiTF318639

Family and domain databases

InterProiView protein in InterPro
IPR002659 Glyco_trans_31
PANTHERiPTHR11214 PTHR11214, 1 hit
PfamiView protein in Pfam
PF01762 Galactosyl_T, 1 hit

Sequencei

Sequence statusi: Complete.

Q99NB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVFVSSRRV KRWQFFHLFA ICFILSFMVF WGPINNYIMS HMKSYSYRYL
60 70 80 90 100
INSYDFVNDS LSLKHSSVQP HHPYLINHRE KCQAQDVLLL LFIKTAPENY
110 120 130 140 150
ERRSAIRKTW GNENYVQSQL NANIKILFAL GTPHPLKGKE LQKRLIWEDQ
160 170 180 190 200
VYHDIIQQDF TDSFHNLTFK FLLQFGWANT FCPHARFLMT ADDDIFIHMP
210 220 230 240 250
NLIEYLQGLE QVGVRDFWIG HVHRGGPPVR DKSSKYYVPY EMYKWPAYPD
260 270 280 290 300
YTAGAAYVVS NDVAAKIYEA SQTLNSSMYI DDVFMGLCAN KVGVVPQDHV
310 320 330 340 350
FFSGEGKIPY HPCIYEKMIT SHGHSQDLQD LWVEATDPKV KDISKGFFGQ
360 370
IYCRLIKIVL LCRLTYRNSY PCRAAFA
Length:377
Mass (Da):44,131
Last modified:May 29, 2007 - v2
Checksum:iEC57827D7BFB5CF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03079736 Genomic DNA No translation available.
AB045279 mRNA Translation: BAB40941.1
RefSeqiNP_446384.1, NM_053932.1
UniGeneiRn.20376

Genome annotation databases

EnsembliENSRNOT00000071848; ENSRNOP00000067153; ENSRNOG00000046258
GeneIDi116740
KEGGirno:116740

Similar proteinsi

Entry informationi

Entry nameiB3GN5_RAT
AccessioniPrimary (citable) accession number: Q99NB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health