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Q99NB2

- B3GN5_RAT

UniProt

Q99NB2 - B3GN5_RAT

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Protein
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Gene
B3gnt5
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc4Cer; paragloboside), resulting in the synthesis of Lc3Cer and neolactopentaosylceramide (nLc5Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development By similarity.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Pathwayi

GO - Molecular functioni

  1. beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity Source: Ensembl
  2. galactosyltransferase activity Source: InterPro
  3. lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity Source: UniProtKB-EC
  4. lipopolysaccharide N-acetylglucosaminyltransferase activity Source: Ensembl

GO - Biological processi

  1. central nervous system development Source: Ensembl
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.206. 5301.
ReactomeiREACT_196259. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase (EC:2.4.1.206)
Alternative name(s):
Lactotriaosylceramide synthase
Short name:
Lc(3)Cer synthase
Short name:
Lc3 synthase
UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
BGnT-5
Short name:
Beta-1,3-Gn-T5
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
Beta3Gn-T5
Gene namesi
Name:B3gnt5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 11

Organism-specific databases

RGDi70955. B3gnt5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini36 – 377342Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
PRO_0000289212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ99NB2.

Expressioni

Gene expression databases

GenevestigatoriQ99NB2.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002616.

Structurei

3D structure databases

ProteinModelPortaliQ99NB2.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253164.
GeneTreeiENSGT00740000115391.
HOGENOMiHOG000064519.
HOVERGENiHBG101684.
InParanoidiQ99NB2.
KOiK03766.
OMAiTWGNEKY.
OrthoDBiEOG7C2R1D.
PhylomeDBiQ99NB2.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99NB2-1 [UniParc]FASTAAdd to Basket

« Hide

MRVFVSSRRV KRWQFFHLFA ICFILSFMVF WGPINNYIMS HMKSYSYRYL    50
INSYDFVNDS LSLKHSSVQP HHPYLINHRE KCQAQDVLLL LFIKTAPENY 100
ERRSAIRKTW GNENYVQSQL NANIKILFAL GTPHPLKGKE LQKRLIWEDQ 150
VYHDIIQQDF TDSFHNLTFK FLLQFGWANT FCPHARFLMT ADDDIFIHMP 200
NLIEYLQGLE QVGVRDFWIG HVHRGGPPVR DKSSKYYVPY EMYKWPAYPD 250
YTAGAAYVVS NDVAAKIYEA SQTLNSSMYI DDVFMGLCAN KVGVVPQDHV 300
FFSGEGKIPY HPCIYEKMIT SHGHSQDLQD LWVEATDPKV KDISKGFFGQ 350
IYCRLIKIVL LCRLTYRNSY PCRAAFA 377
Length:377
Mass (Da):44,131
Last modified:May 29, 2007 - v2
Checksum:iEC57827D7BFB5CF9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AABR03079736 Genomic DNA. No translation available.
AB045279 mRNA. Translation: BAB40941.1.
RefSeqiNP_446384.1. NM_053932.1.
UniGeneiRn.20376.

Genome annotation databases

EnsembliENSRNOT00000071848; ENSRNOP00000067153; ENSRNOG00000046258.
GeneIDi116740.
KEGGirno:116740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AABR03079736 Genomic DNA. No translation available.
AB045279 mRNA. Translation: BAB40941.1 .
RefSeqi NP_446384.1. NM_053932.1.
UniGenei Rn.20376.

3D structure databases

ProteinModelPortali Q99NB2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000002616.

Protein family/group databases

CAZyi GT31. Glycosyltransferase Family 31.

Proteomic databases

PRIDEi Q99NB2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000071848 ; ENSRNOP00000067153 ; ENSRNOG00000046258 .
GeneIDi 116740.
KEGGi rno:116740.

Organism-specific databases

CTDi 84002.
RGDi 70955. B3gnt5.

Phylogenomic databases

eggNOGi NOG253164.
GeneTreei ENSGT00740000115391.
HOGENOMi HOG000064519.
HOVERGENi HBG101684.
InParanoidi Q99NB2.
KOi K03766.
OMAi TWGNEKY.
OrthoDBi EOG7C2R1D.
PhylomeDBi Q99NB2.
TreeFami TF318639.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.206. 5301.
Reactomei REACT_196259. O-linked glycosylation of mucins.

Miscellaneous databases

NextBioi 619666.
PROi Q99NB2.

Gene expression databases

Genevestigatori Q99NB2.

Family and domain databases

InterProi IPR002659. Glyco_trans_31.
[Graphical view ]
PANTHERi PTHR11214. PTHR11214. 1 hit.
Pfami PF01762. Galactosyl_T. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids."
    Togayachi A., Akashima T., Ookubo R., Kudo T., Nishihara S., Iwasaki H., Natsume A., Mio H., Inokuchi J., Irimura T., Sasaki K., Narimatsu H.
    J. Biol. Chem. 276:22032-22040(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-344.

Entry informationi

Entry nameiB3GN5_RAT
AccessioniPrimary (citable) accession number: Q99NB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: September 3, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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