Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99NB2

- B3GN5_RAT

UniProt

Q99NB2 - B3GN5_RAT

Protein

Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Gene

B3gnt5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (29 May 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc4Cer; paragloboside), resulting in the synthesis of Lc3Cer and neolactopentaosylceramide (nLc5Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development By similarity.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

    Pathwayi

    GO - Molecular functioni

    1. beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity Source: Ensembl
    2. galactosyltransferase activity Source: InterPro
    3. lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity Source: UniProtKB-EC
    4. lipopolysaccharide N-acetylglucosaminyltransferase activity Source: Ensembl

    GO - Biological processi

    1. central nervous system development Source: Ensembl
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Developmental protein, Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.206. 5301.
    ReactomeiREACT_196259. O-linked glycosylation of mucins.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT31. Glycosyltransferase Family 31.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase (EC:2.4.1.206)
    Alternative name(s):
    Lactotriaosylceramide synthase
    Short name:
    Lc(3)Cer synthase
    Short name:
    Lc3 synthase
    UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
    Short name:
    BGnT-5
    Short name:
    Beta-1,3-Gn-T5
    Short name:
    Beta-1,3-N-acetylglucosaminyltransferase 5
    Short name:
    Beta3Gn-T5
    Gene namesi
    Name:B3gnt5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 11

    Organism-specific databases

    RGDi70955. B3gnt5.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 377377Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferasePRO_0000289212Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ99NB2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ99NB2.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000002616.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99NB2.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 377342LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 31 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG253164.
    GeneTreeiENSGT00740000115391.
    HOGENOMiHOG000064519.
    HOVERGENiHBG101684.
    InParanoidiQ99NB2.
    KOiK03766.
    OMAiTWGNEKY.
    OrthoDBiEOG7C2R1D.
    PhylomeDBiQ99NB2.
    TreeFamiTF318639.

    Family and domain databases

    InterProiIPR002659. Glyco_trans_31.
    [Graphical view]
    PANTHERiPTHR11214. PTHR11214. 1 hit.
    PfamiPF01762. Galactosyl_T. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99NB2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVFVSSRRV KRWQFFHLFA ICFILSFMVF WGPINNYIMS HMKSYSYRYL    50
    INSYDFVNDS LSLKHSSVQP HHPYLINHRE KCQAQDVLLL LFIKTAPENY 100
    ERRSAIRKTW GNENYVQSQL NANIKILFAL GTPHPLKGKE LQKRLIWEDQ 150
    VYHDIIQQDF TDSFHNLTFK FLLQFGWANT FCPHARFLMT ADDDIFIHMP 200
    NLIEYLQGLE QVGVRDFWIG HVHRGGPPVR DKSSKYYVPY EMYKWPAYPD 250
    YTAGAAYVVS NDVAAKIYEA SQTLNSSMYI DDVFMGLCAN KVGVVPQDHV 300
    FFSGEGKIPY HPCIYEKMIT SHGHSQDLQD LWVEATDPKV KDISKGFFGQ 350
    IYCRLIKIVL LCRLTYRNSY PCRAAFA 377
    Length:377
    Mass (Da):44,131
    Last modified:May 29, 2007 - v2
    Checksum:iEC57827D7BFB5CF9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AABR03079736 Genomic DNA. No translation available.
    AB045279 mRNA. Translation: BAB40941.1.
    RefSeqiNP_446384.1. NM_053932.1.
    UniGeneiRn.20376.

    Genome annotation databases

    EnsembliENSRNOT00000071848; ENSRNOP00000067153; ENSRNOG00000046258.
    GeneIDi116740.
    KEGGirno:116740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AABR03079736 Genomic DNA. No translation available.
    AB045279 mRNA. Translation: BAB40941.1 .
    RefSeqi NP_446384.1. NM_053932.1.
    UniGenei Rn.20376.

    3D structure databases

    ProteinModelPortali Q99NB2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000002616.

    Protein family/group databases

    CAZyi GT31. Glycosyltransferase Family 31.

    Proteomic databases

    PRIDEi Q99NB2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000071848 ; ENSRNOP00000067153 ; ENSRNOG00000046258 .
    GeneIDi 116740.
    KEGGi rno:116740.

    Organism-specific databases

    CTDi 84002.
    RGDi 70955. B3gnt5.

    Phylogenomic databases

    eggNOGi NOG253164.
    GeneTreei ENSGT00740000115391.
    HOGENOMi HOG000064519.
    HOVERGENi HBG101684.
    InParanoidi Q99NB2.
    KOi K03766.
    OMAi TWGNEKY.
    OrthoDBi EOG7C2R1D.
    PhylomeDBi Q99NB2.
    TreeFami TF318639.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 2.4.1.206. 5301.
    Reactomei REACT_196259. O-linked glycosylation of mucins.

    Miscellaneous databases

    NextBioi 619666.
    PROi Q99NB2.

    Gene expression databases

    Genevestigatori Q99NB2.

    Family and domain databases

    InterProi IPR002659. Glyco_trans_31.
    [Graphical view ]
    PANTHERi PTHR11214. PTHR11214. 1 hit.
    Pfami PF01762. Galactosyl_T. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
      , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
      Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    2. "Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids."
      Togayachi A., Akashima T., Ookubo R., Kudo T., Nishihara S., Iwasaki H., Natsume A., Mio H., Inokuchi J., Irimura T., Sasaki K., Narimatsu H.
      J. Biol. Chem. 276:22032-22040(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-344.

    Entry informationi

    Entry nameiB3GN5_RAT
    AccessioniPrimary (citable) accession number: Q99NB2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3