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Protein

Acetyl-coenzyme A synthetase 2-like, mitochondrial

Gene

Acss1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO2.3 Publications

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.

Enzyme regulationi

Inhibited by acetylation at Lys-635 and activated by deacetylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei334Coenzyme ABy similarity1
Binding sitei526ATPBy similarity1
Binding sitei541ATPBy similarity1
Binding sitei549Coenzyme A; via carbonyl oxygenBy similarity1
Binding sitei552ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi410 – 412ATPBy similarity3
Nucleotide bindingi434 – 439ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.1. 3474.
ReactomeiR-MMU-71384. Ethanol oxidation.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetase 2-like, mitochondrial (EC:6.2.1.1)
Alternative name(s):
Acetate--CoA ligase 2
Acetyl-CoA synthetase 2
Short name:
AceCS2
Acyl-CoA synthetase short-chain family member 1
Gene namesi
Name:Acss1
Synonyms:Acas2, Acas2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1915988. Acss1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No visible phenotype at birth, but exhibit significant growth retardation at the time of weaning. Attain normal size and weight when fed normally. Exhibit hypothermia and hypoglycemia when fed high-fat, low-carbohydrate diet, leading to 50% mortality. Display strongly reduced whole-body acetate oxidation when fasting. Fasting adults exhibit hypothermia, reduced capacity to sustain running and low ATP levels.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38MitochondrionSequence analysisAdd BLAST38
ChainiPRO_000000059739 – 682Acetyl-coenzyme A synthetase 2-like, mitochondrialAdd BLAST644

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei389N6-acetyllysineBy similarity1
Modified residuei635N6-acetyllysine1 Publication1

Post-translational modificationi

Reversibly acetylated at Lys-635. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99NB1.
MaxQBiQ99NB1.
PaxDbiQ99NB1.
PRIDEiQ99NB1.

PTM databases

iPTMnetiQ99NB1.
PhosphoSitePlusiQ99NB1.

Expressioni

Tissue specificityi

Highly expressed in heart, testis, kidney, skeletal muscle, lung and spleen. Detected at low levels in brain.

Inductioni

By fasting.

Gene expression databases

BgeeiENSMUSG00000027452.
CleanExiMM_ACSS1.
ExpressionAtlasiQ99NB1. baseline and differential.
GenevisibleiQ99NB1. MM.

Interactioni

Subunit structurei

Interacts with SIRT3.1 Publication

Protein-protein interaction databases

BioGridi213026. 1 interactor.
DIPiDIP-61209N.
IntActiQ99NB1. 2 interactors.
MINTiMINT-4086737.
STRINGi10090.ENSMUSP00000028944.

Structurei

3D structure databases

ProteinModelPortaliQ99NB1.
SMRiQ99NB1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 220Coenzyme A bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOVERGENiHBG014401.
InParanoidiQ99NB1.
KOiK01895.
OMAiSSGKIGW.
OrthoDBiEOG091G039C.
TreeFamiTF354241.

Family and domain databases

CDDicd05966. ACS. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99NB1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARSLGSGV GRLLRGLQGR SGQSGWSLSV SRSTATRLPG CVPAAAQPGS
60 70 80 90 100
YPALSAQAAQ EPAAFWGPLA RDTLVWDTPY HTVWDCDFRT GKIGWFLGGQ
110 120 130 140 150
LNVSVNCLDQ HVQKSPETIA LIWERDEPGT EVRITYRELL ETTCRLANTL
160 170 180 190 200
KRHGVHRGDR VAIYMPVSPL AVAAMLACAR IGAIHTVVFA GFSAESLAGR
210 220 230 240 250
INDAKCKAVI TFNQGLRGGR VVELKKIVDE AVKSCPTVQH VLVAHRTDTK
260 270 280 290 300
VPMGSLDIPL EQEMAKEAPV CTPESMSSED MLFMLYTSGS TGTPKGLVHT
310 320 330 340 350
QAGYLLYAAM THKLVFDYQP GDVFGCVADI GWITGHSYVV YGPLCNGATT
360 370 380 390 400
VLFESTPVYP DAGRYWETVQ RLKINQFYGA PTAVRLLLKY GDAWVKKYDR
410 420 430 440 450
SSLRTLGSVG EPINHEAWEW LHKVVGDGRC TLVDTWWQTE TGGICIAPRP
460 470 480 490 500
SEDGAEILPG MAMRPFFGIV PVLMDEKGNV LEGGDVSGAL CISQAWPGMA
510 520 530 540 550
RTIYGDHQRF VDAYFRAYPG YYFTGDGAHR TEGGYYQITG RMDDVINISG
560 570 580 590 600
HRLGTAEIED AMADHPAVPE TAVIGYPHDI KGEAAFAFIV LKDNISDENM
610 620 630 640 650
VVNELKLSVA TKIAKYAVPD QILVVKRLPK TRSGKVMRRL LRKIITSRGQ
660 670 680
DLGDTTTLED PSVITEILSA FQKYEEQRAA TN
Length:682
Mass (Da):74,623
Last modified:June 1, 2001 - v1
Checksum:i1B21981D29F9C8E7
GO

Sequence cautioni

The sequence AAH30930 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti332W → C in AAH30930 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046742 mRNA. Translation: BAB21612.1.
AK088244 mRNA. Translation: BAC40232.1.
BC030930 mRNA. Translation: AAH30930.1. Different initiation.
CCDSiCCDS16859.1.
RefSeqiNP_542142.1. NM_080575.2.
UniGeneiMm.7044.

Genome annotation databases

EnsembliENSMUST00000028944; ENSMUSP00000028944; ENSMUSG00000027452.
GeneIDi68738.
KEGGimmu:68738.
UCSCiuc008muf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046742 mRNA. Translation: BAB21612.1.
AK088244 mRNA. Translation: BAC40232.1.
BC030930 mRNA. Translation: AAH30930.1. Different initiation.
CCDSiCCDS16859.1.
RefSeqiNP_542142.1. NM_080575.2.
UniGeneiMm.7044.

3D structure databases

ProteinModelPortaliQ99NB1.
SMRiQ99NB1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213026. 1 interactor.
DIPiDIP-61209N.
IntActiQ99NB1. 2 interactors.
MINTiMINT-4086737.
STRINGi10090.ENSMUSP00000028944.

PTM databases

iPTMnetiQ99NB1.
PhosphoSitePlusiQ99NB1.

Proteomic databases

EPDiQ99NB1.
MaxQBiQ99NB1.
PaxDbiQ99NB1.
PRIDEiQ99NB1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028944; ENSMUSP00000028944; ENSMUSG00000027452.
GeneIDi68738.
KEGGimmu:68738.
UCSCiuc008muf.2. mouse.

Organism-specific databases

CTDi84532.
MGIiMGI:1915988. Acss1.

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOVERGENiHBG014401.
InParanoidiQ99NB1.
KOiK01895.
OMAiSSGKIGW.
OrthoDBiEOG091G039C.
TreeFamiTF354241.

Enzyme and pathway databases

BRENDAi6.2.1.1. 3474.
ReactomeiR-MMU-71384. Ethanol oxidation.

Miscellaneous databases

PROiQ99NB1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027452.
CleanExiMM_ACSS1.
ExpressionAtlasiQ99NB1. baseline and differential.
GenevisibleiQ99NB1. MM.

Family and domain databases

CDDicd05966. ACS. 1 hit.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACS2L_MOUSE
AccessioniPrimary (citable) accession number: Q99NB1
Secondary accession number(s): Q8K0M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.