Reviewed, UniProtKB/Swiss-Prot Q99NA5 (IDH3A_RAT)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

Idh3a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion By similarity.
Tissue specificity	Expressed in brown adipose tissue (BAT). Ref.1
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	2-oxoglutarate metabolic process Inferred from direct assay. Source: RGD NADH metabolic process Inferred from direct assay. Source: RGD isocitrate metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from direct assay. Source: RGD
Cellular component	mitochondrial isocitrate dehydrogenase complex (NAD+) Inferred by curator. Source: RGD
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Ref.1 Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion By similarity
	Chain	28 – 366	339	Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014440
Sites
	Metal binding	233	1	Magnesium or manganese By similarity
	Metal binding	257	1	Magnesium or manganese By similarity
	Metal binding	261	1	Magnesium or manganese By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity
	Site	153	1	Critical for catalysis By similarity
	Site	200	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99NA5-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: DE5BA27AE3CF0846	FASTA	366	39,614
	10 20 30 40 50 60 MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV MKIFDAAKAP 70 80 90 100 110 120 IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK TPIAAGHPSM NLLLRKTFDL 130 140 150 160 170 180 YANVRPCVSI EGYKTPYTDV NIVTIRENTE GEYSGIEHVI VDGVVQSIKL ITEGASKRIA 190 200 210 220 230 240 EFAFEYARNN HRSNVTAVHK ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV 250 260 270 280 290 300 QDPSQFDVLV MPNLYGDILS DLCAGLIGGL GVTQSGNIGA NGVAIFESVH GTAPDIAGKD 310 320 330 340 350 360 MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNSK CSDFTEEICR RVKDLD

References

[1]	"Expression of NAD+-dependent isocitrate dehydrogenase in brown adipose tissue." Shinohara Y., Daikoku T., Kajimoto K., Shima A., Yamazaki N., Terada H. Biochem. Biophys. Res. Commun. 281:634-638(2001) [PubMed: 11237704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 67-77; 101-146; 179-188; 206-214; 327-336 AND 351-361, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047541 mRNA. Translation: BAB32675.1.
IPI	IPI00198720.
RefSeq	NP_446090.1.
UniGene	Rn.95104
3D structure databases
HSSP	HSSP built from PDB template 1ISO based on UniProtKB P08200.
ModBase	Search...
Proteomic databases
PRIDE	Q99NA5.
Genome annotation databases
Ensembl	ENSRNOG00000010277. Rattus norvegicus. [Contig view]
GeneID	114096.
KEGG	rno:114096.
Organism-specific databases
RGD	70889. Idh3a.
Phylogenomic databases
HOVERGEN	Q99NA5.
Enzyme and pathway databases
BRENDA	1.1.1.41. 248.
Gene expression databases
ArrayExpress	Q99NA5.
GermOnline	ENSRNOG00000010277. Rattus norvegicus.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618241.

Entry information

Entry name

IDH3A_RAT

Accession

Primary (citable) accession number: Q99NA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)