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Protein

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Gene

Idh3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115SubstrateBy similarity1
Binding sitei125SubstrateBy similarity1
Binding sitei146SubstrateBy similarity1
Sitei153Critical for catalysisBy similarity1
Sitei200Critical for catalysisBy similarity1
Metal bindingi233Magnesium or manganeseBy similarity1
Binding sitei233SubstrateBy similarity1
Metal bindingi257Magnesium or manganeseBy similarity1
Metal bindingi261Magnesium or manganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 2-oxoglutarate metabolic process Source: RGD
  • isocitrate metabolic process Source: RGD
  • NADH metabolic process Source: RGD
  • tricarboxylic acid cycle Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

SABIO-RKQ99NA5.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha
Gene namesi
Name:Idh3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70889. Idh3a.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial isocitrate dehydrogenase complex (NAD+) Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000001444028 – 366Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialAdd BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77N6-succinyllysineBy similarity1
Modified residuei101PhosphothreonineBy similarity1
Modified residuei223N6-acetyllysineBy similarity1
Modified residuei343N6-acetyllysine; alternateBy similarity1
Modified residuei343N6-succinyllysine; alternateBy similarity1
Modified residuei350N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ99NA5.
PRIDEiQ99NA5.

2D gel databases

World-2DPAGE0004:Q99NA5.

PTM databases

iPTMnetiQ99NA5.
PhosphoSitePlusiQ99NA5.

Expressioni

Tissue specificityi

Expressed in brown adipose tissue (BAT).1 Publication

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Protein-protein interaction databases

BioGridi250275. 1 interactor.
IntActiQ99NA5. 1 interactor.
STRINGi10116.ENSRNOP00000015102.

Structurei

3D structure databases

ProteinModelPortaliQ99NA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0785. Eukaryota.
COG0473. LUCA.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ99NA5.
KOiK00030.
PhylomeDBiQ99NA5.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99NA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV
60 70 80 90 100
MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK
110 120 130 140 150
TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE
160 170 180 190 200
GEYSGIEHVI VDGVVQSIKL ITEGASKRIA EFAFEYARNN HRSNVTAVHK
210 220 230 240 250
ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV QDPSQFDVLV
260 270 280 290 300
MPNLYGDILS DLCAGLIGGL GVTQSGNIGA NGVAIFESVH GTAPDIAGKD
310 320 330 340 350
MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNSK
360
CSDFTEEICR RVKDLD
Length:366
Mass (Da):39,614
Last modified:June 1, 2001 - v1
Checksum:iDE5BA27AE3CF0846
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047541 mRNA. Translation: BAB32675.1.
RefSeqiNP_446090.1. NM_053638.1.
UniGeneiRn.95104.

Genome annotation databases

GeneIDi114096.
KEGGirno:114096.
UCSCiRGD:70889. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047541 mRNA. Translation: BAB32675.1.
RefSeqiNP_446090.1. NM_053638.1.
UniGeneiRn.95104.

3D structure databases

ProteinModelPortaliQ99NA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250275. 1 interactor.
IntActiQ99NA5. 1 interactor.
STRINGi10116.ENSRNOP00000015102.

PTM databases

iPTMnetiQ99NA5.
PhosphoSitePlusiQ99NA5.

2D gel databases

World-2DPAGE0004:Q99NA5.

Proteomic databases

PaxDbiQ99NA5.
PRIDEiQ99NA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114096.
KEGGirno:114096.
UCSCiRGD:70889. rat.

Organism-specific databases

CTDi3419.
RGDi70889. Idh3a.

Phylogenomic databases

eggNOGiKOG0785. Eukaryota.
COG0473. LUCA.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ99NA5.
KOiK00030.
PhylomeDBiQ99NA5.

Enzyme and pathway databases

SABIO-RKQ99NA5.

Miscellaneous databases

PROiQ99NA5.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDH3A_RAT
AccessioniPrimary (citable) accession number: Q99NA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.