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Protein

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Gene

Idh3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151SubstrateBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Binding sitei146 – 1461SubstrateBy similarity
Sitei153 – 1531Critical for catalysisBy similarity
Sitei200 – 2001Critical for catalysisBy similarity
Metal bindingi233 – 2331Magnesium or manganeseBy similarity
Binding sitei233 – 2331SubstrateBy similarity
Metal bindingi257 – 2571Magnesium or manganeseBy similarity
Metal bindingi261 – 2611Magnesium or manganeseBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NAD+) activity Source: RGD
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: RGD
  2. isocitrate metabolic process Source: RGD
  3. NADH metabolic process Source: RGD
  4. tricarboxylic acid cycle Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

SABIO-RKQ99NA5.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha
Gene namesi
Name:Idh3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi70889. Idh3a.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial isocitrate dehydrogenase complex (NAD+) Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionBy similarityAdd
BLAST
Chaini28 – 366339Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialPRO_0000014440Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771N6-succinyllysineBy similarity
Modified residuei223 – 2231N6-acetyllysineBy similarity
Modified residuei343 – 3431N6-acetyllysine; alternateBy similarity
Modified residuei343 – 3431N6-succinyllysine; alternateBy similarity
Modified residuei350 – 3501N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ99NA5.
PRIDEiQ99NA5.

2D gel databases

World-2DPAGE0004:Q99NA5.

Expressioni

Tissue specificityi

Expressed in brown adipose tissue (BAT).1 Publication

Gene expression databases

GenevestigatoriQ99NA5.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Protein-protein interaction databases

IntActiQ99NA5. 1 interaction.
STRINGi10116.ENSRNOP00000015102.

Structurei

3D structure databases

ProteinModelPortaliQ99NA5.
SMRiQ99NA5. Positions 26-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ99NA5.
KOiK00030.
PhylomeDBiQ99NA5.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99NA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV
60 70 80 90 100
MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK
110 120 130 140 150
TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE
160 170 180 190 200
GEYSGIEHVI VDGVVQSIKL ITEGASKRIA EFAFEYARNN HRSNVTAVHK
210 220 230 240 250
ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV QDPSQFDVLV
260 270 280 290 300
MPNLYGDILS DLCAGLIGGL GVTQSGNIGA NGVAIFESVH GTAPDIAGKD
310 320 330 340 350
MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNSK
360
CSDFTEEICR RVKDLD
Length:366
Mass (Da):39,614
Last modified:June 1, 2001 - v1
Checksum:iDE5BA27AE3CF0846
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047541 mRNA. Translation: BAB32675.1.
RefSeqiNP_446090.1. NM_053638.1.
UniGeneiRn.95104.

Genome annotation databases

GeneIDi114096.
KEGGirno:114096.
UCSCiRGD:70889. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047541 mRNA. Translation: BAB32675.1.
RefSeqiNP_446090.1. NM_053638.1.
UniGeneiRn.95104.

3D structure databases

ProteinModelPortaliQ99NA5.
SMRiQ99NA5. Positions 26-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99NA5. 1 interaction.
STRINGi10116.ENSRNOP00000015102.

2D gel databases

World-2DPAGE0004:Q99NA5.

Proteomic databases

PaxDbiQ99NA5.
PRIDEiQ99NA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114096.
KEGGirno:114096.
UCSCiRGD:70889. rat.

Organism-specific databases

CTDi3419.
RGDi70889. Idh3a.

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ99NA5.
KOiK00030.
PhylomeDBiQ99NA5.

Enzyme and pathway databases

SABIO-RKQ99NA5.

Miscellaneous databases

NextBioi618241.

Gene expression databases

GenevestigatoriQ99NA5.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of NAD+-dependent isocitrate dehydrogenase in brown adipose tissue."
    Shinohara Y., Daikoku T., Kajimoto K., Shima A., Yamazaki N., Terada H.
    Biochem. Biophys. Res. Commun. 281:634-638(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 67-77; 101-146; 179-188; 206-214; 327-336 AND 351-361, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiIDH3A_RAT
AccessioniPrimary (citable) accession number: Q99NA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: March 4, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.