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Q99NA5

- IDH3A_RAT

UniProt

Q99NA5 - IDH3A_RAT

Protein

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Gene

Idh3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei115 – 1151SubstrateBy similarity
    Binding sitei125 – 1251SubstrateBy similarity
    Binding sitei146 – 1461SubstrateBy similarity
    Sitei153 – 1531Critical for catalysisBy similarity
    Sitei200 – 2001Critical for catalysisBy similarity
    Metal bindingi233 – 2331Magnesium or manganeseBy similarity
    Binding sitei233 – 2331SubstrateBy similarity
    Metal bindingi257 – 2571Magnesium or manganeseBy similarity
    Metal bindingi261 – 2611Magnesium or manganeseBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: RGD
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. 2-oxoglutarate metabolic process Source: RGD
    2. isocitrate metabolic process Source: RGD
    3. NADH metabolic process Source: RGD
    4. tricarboxylic acid cycle Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    SABIO-RKQ99NA5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    Isocitric dehydrogenase subunit alpha
    NAD(+)-specific ICDH subunit alpha
    Gene namesi
    Name:Idh3a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi70889. Idh3a.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrial isocitrate dehydrogenase complex (NAD+) Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionBy similarityAdd
    BLAST
    Chaini28 – 366339Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialPRO_0000014440Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771N6-succinyllysineBy similarity
    Modified residuei223 – 2231N6-acetyllysineBy similarity
    Modified residuei343 – 3431N6-acetyllysine; alternateBy similarity
    Modified residuei343 – 3431N6-succinyllysine; alternateBy similarity
    Modified residuei350 – 3501N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ99NA5.
    PRIDEiQ99NA5.

    2D gel databases

    World-2DPAGE0004:Q99NA5.

    Expressioni

    Tissue specificityi

    Expressed in brown adipose tissue (BAT).1 Publication

    Gene expression databases

    GenevestigatoriQ99NA5.

    Interactioni

    Subunit structurei

    Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

    Protein-protein interaction databases

    IntActiQ99NA5. 1 interaction.
    STRINGi10116.ENSRNOP00000015102.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99NA5.
    SMRiQ99NA5. Positions 26-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021113.
    HOVERGENiHBG052080.
    InParanoidiQ99NA5.
    KOiK00030.
    PhylomeDBiQ99NA5.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99NA5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV    50
    MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK 100
    TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE 150
    GEYSGIEHVI VDGVVQSIKL ITEGASKRIA EFAFEYARNN HRSNVTAVHK 200
    ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV QDPSQFDVLV 250
    MPNLYGDILS DLCAGLIGGL GVTQSGNIGA NGVAIFESVH GTAPDIAGKD 300
    MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNSK 350
    CSDFTEEICR RVKDLD 366
    Length:366
    Mass (Da):39,614
    Last modified:June 1, 2001 - v1
    Checksum:iDE5BA27AE3CF0846
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB047541 mRNA. Translation: BAB32675.1.
    RefSeqiNP_446090.1. NM_053638.1.
    UniGeneiRn.95104.

    Genome annotation databases

    GeneIDi114096.
    KEGGirno:114096.
    UCSCiRGD:70889. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB047541 mRNA. Translation: BAB32675.1 .
    RefSeqi NP_446090.1. NM_053638.1.
    UniGenei Rn.95104.

    3D structure databases

    ProteinModelPortali Q99NA5.
    SMRi Q99NA5. Positions 26-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99NA5. 1 interaction.
    STRINGi 10116.ENSRNOP00000015102.

    2D gel databases

    World-2DPAGE 0004:Q99NA5.

    Proteomic databases

    PaxDbi Q99NA5.
    PRIDEi Q99NA5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 114096.
    KEGGi rno:114096.
    UCSCi RGD:70889. rat.

    Organism-specific databases

    CTDi 3419.
    RGDi 70889. Idh3a.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021113.
    HOVERGENi HBG052080.
    InParanoidi Q99NA5.
    KOi K00030.
    PhylomeDBi Q99NA5.

    Enzyme and pathway databases

    SABIO-RK Q99NA5.

    Miscellaneous databases

    NextBioi 618241.

    Gene expression databases

    Genevestigatori Q99NA5.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of NAD+-dependent isocitrate dehydrogenase in brown adipose tissue."
      Shinohara Y., Daikoku T., Kajimoto K., Shima A., Yamazaki N., Terada H.
      Biochem. Biophys. Res. Commun. 281:634-638(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 67-77; 101-146; 179-188; 206-214; 327-336 AND 351-361, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiIDH3A_RAT
    AccessioniPrimary (citable) accession number: Q99NA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3