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Protein

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Gene

Srd5a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology (By similarity).By similarity

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: MGI
  2. amide binding Source: Ensembl
  3. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  4. sterol 5-alpha reductase activity Source: MGI

GO - Biological processi

  1. androgen biosynthetic process Source: MGI
  2. androgen metabolic process Source: MGI
  3. biphenyl metabolic process Source: Ensembl
  4. bone development Source: Ensembl
  5. cell differentiation Source: UniProtKB-KW
  6. dibenzo-p-dioxin metabolic process Source: Ensembl
  7. female genitalia development Source: Ensembl
  8. hippocampus development Source: Ensembl
  9. hypothalamus development Source: Ensembl
  10. male genitalia development Source: MGI
  11. male gonad development Source: MGI
  12. phthalate metabolic process Source: Ensembl
  13. response to drug Source: Ensembl
  14. response to follicle-stimulating hormone Source: Ensembl
  15. response to nutrient levels Source: Ensembl
  16. response to peptide hormone Source: Ensembl
  17. response to testosterone Source: Ensembl
  18. steroid biosynthetic process Source: MGI
  19. steroid catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Differentiation, Sexual differentiation

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 2 (EC:1.3.1.22)
Alternative name(s):
5 alpha-SR2
SR type 2
Steroid 5-alpha-reductase 2
Short name:
S5AR 2
Gene namesi
Name:Srd5a2
Synonyms:5art2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2150380. Srd5a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence AnalysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence AnalysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell body fiber Source: Ensembl
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. neuronal cell body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2542543-oxo-5-alpha-steroid 4-dehydrogenase 2PRO_0000213678Add
BLAST

Proteomic databases

PRIDEiQ99N99.

PTM databases

PhosphoSiteiQ99N99.

Expressioni

Gene expression databases

BgeeiQ99N99.
CleanExiMM_SRD5A2.
ExpressionAtlasiQ99N99. baseline and differential.
GenevestigatoriQ99N99.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048862.

Structurei

3D structure databases

ProteinModelPortaliQ99N99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG282706.
GeneTreeiENSGT00510000046634.
HOGENOMiHOG000050133.
HOVERGENiHBG003402.
InParanoidiQ99N99.
KOiK12344.
OMAiHHRYYLK.
OrthoDBiEOG75QR56.
PhylomeDBiQ99N99.
TreeFamiTF314668.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99N99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIVCHQVPV LAGSATLATM GTLILCFGKP ASYGKHSESV SSGVPLLPAR
60 70 80 90 100
IAWFLQELPS FVVSVGMLAW QPRSLFGPPG NVLLGLFSAH YFHRTFIYSL
110 120 130 140 150
LTRGRPLSAV IFLKATAFCI GNGLLQAYYL VYCAEYPEEW YTDMRFSVGV
160 170 180 190 200
FFFILGMGIN IHSDCMLRQL RKPGEVIYRI PQGGLFTYVS GANFLGEIIE
210 220 230 240 250
WMGYALATWS VPAFAFAFFT LCFLGMQAFY HHRFYLKMFK DYPKSRKALI

PFIF
Length:254
Mass (Da):28,619
Last modified:June 1, 2001 - v1
Checksum:i8D25019E8DC4DF47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049456 mRNA. Translation: BAB40179.1.
AK138946 mRNA. Translation: BAE23830.1.
BC125510 mRNA. Translation: AAI25511.1.
CCDSiCCDS28968.1.
RefSeqiNP_444418.1. NM_053188.2.
UniGeneiMm.38933.

Genome annotation databases

EnsembliENSMUST00000043458; ENSMUSP00000048862; ENSMUSG00000038541.
GeneIDi94224.
KEGGimmu:94224.
UCSCiuc008dnt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049456 mRNA. Translation: BAB40179.1.
AK138946 mRNA. Translation: BAE23830.1.
BC125510 mRNA. Translation: AAI25511.1.
CCDSiCCDS28968.1.
RefSeqiNP_444418.1. NM_053188.2.
UniGeneiMm.38933.

3D structure databases

ProteinModelPortaliQ99N99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048862.

PTM databases

PhosphoSiteiQ99N99.

Proteomic databases

PRIDEiQ99N99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043458; ENSMUSP00000048862; ENSMUSG00000038541.
GeneIDi94224.
KEGGimmu:94224.
UCSCiuc008dnt.1. mouse.

Organism-specific databases

CTDi6716.
MGIiMGI:2150380. Srd5a2.

Phylogenomic databases

eggNOGiNOG282706.
GeneTreeiENSGT00510000046634.
HOGENOMiHOG000050133.
HOVERGENiHBG003402.
InParanoidiQ99N99.
KOiK12344.
OMAiHHRYYLK.
OrthoDBiEOG75QR56.
PhylomeDBiQ99N99.
TreeFamiTF314668.

Miscellaneous databases

NextBioi352223.
PROiQ99N99.
SOURCEiSearch...

Gene expression databases

BgeeiQ99N99.
CleanExiMM_SRD5A2.
ExpressionAtlasiQ99N99. baseline and differential.
GenevestigatoriQ99N99.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Transcriptional regulation of the mouse steroid 5alpha-reductase type II gene by progesterone in brain."
    Takeyama K., Kato S.
    Nucleic Acids Res. 30:1387-1393(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Aorta and Vein.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiS5A2_MOUSE
AccessioniPrimary (citable) accession number: Q99N99
Secondary accession number(s): Q3UTZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.