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Protein

Kremen protein 1

Gene

Kremen1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Kremen protein 1
Alternative name(s):
Dickkopf receptor
Kringle domain-containing transmembrane protein 1
Kringle-containing protein marking the eye and the nose
Gene namesi
Name:Kremen1
Synonyms:Kremen
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1933988. Kremen1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 392372ExtracellularSequence analysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence analysisAdd
BLAST
Topological domaini414 – 47360CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 473454Kremen protein 1PRO_0000021565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 114By similarity
Disulfide bondi55 ↔ 95By similarity
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence analysis
Disulfide bondi84 ↔ 109By similarity
Disulfide bondi214 ↔ 240By similarity
Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence analysis
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ99N43.
PRIDEiQ99N43.

PTM databases

PhosphoSiteiQ99N43.

Expressioni

Tissue specificityi

In the adult, widely expressed with high levels in heart, lung, kidney, skeletal muscle and testis.1 Publication

Developmental stagei

In the embryo, expression is first detected on day 9 and increases up to day 18. Lower levels are found in adult. At 9.5 dpc, expression is localised to the apical ectodermal ridge (AER) of the developing fore- and hindlimb buds, the telencephalon and the first brachial arch. At 10.5 dpc, expression is also observed in the myotome and in sensory tissues such as the nasal pit and optic vesicle.1 Publication

Gene expression databases

BgeeiQ99N43.
CleanExiMM_KREMEN1.
ExpressionAtlasiQ99N43. baseline and differential.
GenevisibleiQ99N43. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020662.

Structurei

3D structure databases

ProteinModelPortaliQ99N43.
SMRiQ99N43. Positions 28-173, 214-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 11484KringlePROSITE-ProRule annotationAdd
BLAST
Domaini116 – 21095WSCPROSITE-ProRule annotationAdd
BLAST
Domaini214 – 321108CUBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CUB domain.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 WSC domain.PROSITE-ProRule annotation

Keywords - Domaini

Kringle, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IR07. Eukaryota.
ENOG41112QM. LUCA.
GeneTreeiENSGT00730000110973.
HOGENOMiHOG000070150.
HOVERGENiHBG052290.
InParanoidiQ99N43.
OMAiPECFTAN.
OrthoDBiEOG7T1RB2.
TreeFamiTF331319.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR017076. Kremen.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR002889. WSC_carb-bd.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
PF00051. Kringle. 1 hit.
PF01822. WSC. 1 hit.
[Graphical view]
PIRSFiPIRSF036961. Kremen. 1 hit.
SMARTiSM00042. CUB. 1 hit.
SM00130. KR. 1 hit.
SM00321. WSC. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS51212. WSC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99N43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPAARLAL LSAAALTLAA RPAPGPRSGP ECFTANGADY RGTQSWTALQ
60 70 80 90 100
GGKPCLFWNE TFQHPYNTLK YPNGEGGLGE HNYCRNPDGD VSPWCYVAEH
110 120 130 140 150
EDGVYWKYCE IPACQMPGNL GCYKDHGNPP PLTGTSKTSN KLTIQTCISF
160 170 180 190 200
CRSQRFKFAG MESGYACFCG NNPDYWKHGE AASTECNSVC FGDHTQPCGG
210 220 230 240 250
DGRIILFDTL VGACGGNYSA MAAVVYSPDF PDTYATGRVC YWTIRVPGAS
260 270 280 290 300
RIHFNFTLFD IRDSADMVEL LDGYTHRVLV RLSGRSRPPL SFNVSLDFVI
310 320 330 340 350
LYFFSDRINQ AQGFAVLYQA TKEEPPQERP AVNQTLAEVI TEQANLSVSA
360 370 380 390 400
AHSSKVLYVI TPSPSHPPQT APGSHSWAPS VGANSHRVEG WTVYGLATLL
410 420 430 440 450
ILTVTAVVAK ILLHVTFKSH RVPASGDLRD CRQPGASGDI WTIFYEPSTT
460 470
ISIFKKKLKG QSQQDDRNPL VSD
Length:473
Mass (Da):51,673
Last modified:July 27, 2011 - v2
Checksum:i5E7A906662B80F7D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381R → K in BAB40968 (PubMed:11267660).Curated
Sequence conflicti248 – 2481G → E in BAB40968 (PubMed:11267660).Curated
Sequence conflicti266 – 2661D → N in BAB40968 (PubMed:11267660).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059617 mRNA. Translation: BAB40968.1.
AK141321 mRNA. Translation: BAE24649.1.
AL662853 Genomic DNA. Translation: CAI24979.1.
BC082546 mRNA. Translation: AAH82546.1.
BC138465 mRNA. Translation: AAI38466.1.
BC138464 mRNA. Translation: AAI38465.1.
CCDSiCCDS24399.1.
RefSeqiNP_115772.2. NM_032396.3.
UniGeneiMm.209989.

Genome annotation databases

EnsembliENSMUST00000020662; ENSMUSP00000020662; ENSMUSG00000020393.
GeneIDi84035.
KEGGimmu:84035.
UCSCiuc007hwh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059617 mRNA. Translation: BAB40968.1.
AK141321 mRNA. Translation: BAE24649.1.
AL662853 Genomic DNA. Translation: CAI24979.1.
BC082546 mRNA. Translation: AAH82546.1.
BC138465 mRNA. Translation: AAI38466.1.
BC138464 mRNA. Translation: AAI38465.1.
CCDSiCCDS24399.1.
RefSeqiNP_115772.2. NM_032396.3.
UniGeneiMm.209989.

3D structure databases

ProteinModelPortaliQ99N43.
SMRiQ99N43. Positions 28-173, 214-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020662.

PTM databases

PhosphoSiteiQ99N43.

Proteomic databases

PaxDbiQ99N43.
PRIDEiQ99N43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020662; ENSMUSP00000020662; ENSMUSG00000020393.
GeneIDi84035.
KEGGimmu:84035.
UCSCiuc007hwh.1. mouse.

Organism-specific databases

CTDi83999.
MGIiMGI:1933988. Kremen1.

Phylogenomic databases

eggNOGiENOG410IR07. Eukaryota.
ENOG41112QM. LUCA.
GeneTreeiENSGT00730000110973.
HOGENOMiHOG000070150.
HOVERGENiHBG052290.
InParanoidiQ99N43.
OMAiPECFTAN.
OrthoDBiEOG7T1RB2.
TreeFamiTF331319.

Miscellaneous databases

ChiTaRSiKremen1. mouse.
NextBioi350870.
PROiQ99N43.
SOURCEiSearch...

Gene expression databases

BgeeiQ99N43.
CleanExiMM_KREMEN1.
ExpressionAtlasiQ99N43. baseline and differential.
GenevisibleiQ99N43. MM.

Family and domain databases

Gene3Di2.60.120.290. 1 hit.
InterProiIPR000859. CUB_dom.
IPR017076. Kremen.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR002889. WSC_carb-bd.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
PF00051. Kringle. 1 hit.
PF01822. WSC. 1 hit.
[Graphical view]
PIRSFiPIRSF036961. Kremen. 1 hit.
SMARTiSM00042. CUB. 1 hit.
SM00130. KR. 1 hit.
SM00321. WSC. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS51212. WSC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein."
    Nakamura T., Aoki S., Kitajima K., Takahashi T., Matsumoto K., Nakamura T.
    Biochim. Biophys. Acta 1518:63-72(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Tissue: Brain and Kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiKREM1_MOUSE
AccessioniPrimary (citable) accession number: Q99N43
Secondary accession number(s): Q640Q6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.