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Protein

Thymidine phosphorylase

Gene

Tymp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).By similarity

Catalytic activityi

Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathway:idTMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes dTMP from thymine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Thymidine phosphorylase (Tymp)
  2. no protein annotated in this organism
This subpathway is part of the pathway dTMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dTMP from thymine, the pathway dTMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei188 – 1881SubstrateBy similarity
Binding sitei203 – 2031SubstrateBy similarity
Binding sitei207 – 2071SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • chemotaxis Source: MGI
  • organ morphogenesis Source: MGI
  • pyrimidine nucleobase metabolic process Source: InterPro
  • pyrimidine nucleoside metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.2.4. 3474.
ReactomeiREACT_295314. Pyrimidine salvage reactions.
REACT_317918. Pyrimidine catabolism.
SABIO-RKQ99N42.
UniPathwayiUPA00578; UER00638.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine phosphorylase (EC:2.4.2.4)
Short name:
TP
Alternative name(s):
TdRPase
Gene namesi
Name:Tymp
Synonyms:Ecgf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1920212. Tymp.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Thymidine phosphorylasePRO_0000059047Add
BLAST

Proteomic databases

PaxDbiQ99N42.
PRIDEiQ99N42.

PTM databases

PhosphoSiteiQ99N42.

Expressioni

Gene expression databases

BgeeiQ99N42.
CleanExiMM_TYMP.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023285.

Structurei

3D structure databases

ProteinModelPortaliQ99N42.
SMRiQ99N42. Positions 18-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0213.
GeneTreeiENSGT00390000009250.
HOGENOMiHOG000047313.
HOVERGENiHBG000082.
InParanoidiQ99N42.
KOiK00758.
OMAiREPRFHA.
OrthoDBiEOG744T97.
PhylomeDBiQ99N42.
TreeFamiTF332198.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
InterProiIPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR018090. Pyrmidine_PPas_bac/euk.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02644. Y_phosphoryl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99N42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPGTPPPS ASGGGGGEPR QLPELIRLKR DGGHLREADI RNFVHAVIDG
60 70 80 90 100
RAQDTQIGAM LMAIRLQGMN LEETSVLTRA LAESGQQLEW PKAWHQQLVD
110 120 130 140 150
KHSTGGVGDK VSLVLAPALA ACGCKVPMIS GRSLGHTGGT LDKLESIPGF
160 170 180 190 200
GVTQSPEQML HILEEVGCCI VGQSAKLVPA DGILYAARDV TATVDSVPLI
210 220 230 240 250
TASILSKKAV EGLSTLVVDV KFGGAAVFPD QEKARELAKM LVRVGVSLGL
260 270 280 290 300
KVAAALTAMD NPLGRSVGHT LEVEEALLCL DGAGPPDLRD LVIRLGGAIL
310 320 330 340 350
WISGQAETQD QGAARVAAAL DDGSARRRFQ LMLSAQGVDP GLAKALCSGS
360 370 380 390 400
PTQRRQLLPH AREQEELLAP ADGIVECVRA LPLARVLHDL GAGRSRAGQP
410 420 430 440 450
IRPGVGAEVL VDVGQCLSRG TPWLRVHLDG PALSSQQRRT LQGALVLSDR
460 470
APFKVPSPFA ELVLPPTIAQ P
Length:471
Mass (Da):49,336
Last modified:June 1, 2001 - v1
Checksum:iC6AC64FC004DFF7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB060274 mRNA. Translation: BAB41208.1.
CCDSiCCDS27747.1.
RefSeqiNP_612175.1. NM_138302.1.
UniGeneiMm.287977.

Genome annotation databases

EnsembliENSMUST00000023285; ENSMUSP00000023285; ENSMUSG00000022615.
GeneIDi72962.
KEGGimmu:72962.
UCSCiuc007xgl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB060274 mRNA. Translation: BAB41208.1.
CCDSiCCDS27747.1.
RefSeqiNP_612175.1. NM_138302.1.
UniGeneiMm.287977.

3D structure databases

ProteinModelPortaliQ99N42.
SMRiQ99N42. Positions 18-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023285.

Chemistry

BindingDBiQ99N42.
ChEMBLiCHEMBL2606.

PTM databases

PhosphoSiteiQ99N42.

Proteomic databases

PaxDbiQ99N42.
PRIDEiQ99N42.

Protocols and materials databases

DNASUi72962.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023285; ENSMUSP00000023285; ENSMUSG00000022615.
GeneIDi72962.
KEGGimmu:72962.
UCSCiuc007xgl.2. mouse.

Organism-specific databases

CTDi1890.
MGIiMGI:1920212. Tymp.

Phylogenomic databases

eggNOGiCOG0213.
GeneTreeiENSGT00390000009250.
HOGENOMiHOG000047313.
HOVERGENiHBG000082.
InParanoidiQ99N42.
KOiK00758.
OMAiREPRFHA.
OrthoDBiEOG744T97.
PhylomeDBiQ99N42.
TreeFamiTF332198.

Enzyme and pathway databases

UniPathwayiUPA00578; UER00638.
BRENDAi2.4.2.4. 3474.
ReactomeiREACT_295314. Pyrimidine salvage reactions.
REACT_317918. Pyrimidine catabolism.
SABIO-RKQ99N42.

Miscellaneous databases

NextBioi337237.
PROiQ99N42.
SOURCEiSearch...

Gene expression databases

BgeeiQ99N42.
CleanExiMM_TYMP.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
InterProiIPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR018090. Pyrmidine_PPas_bac/euk.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02644. Y_phosphoryl. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Mouse thymidine phosphorylase."
    Ishikawa F., Toyoshima H., Miyazono K., Haraguchi M.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTYPH_MOUSE
AccessioniPrimary (citable) accession number: Q99N42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.