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Protein

Rho GTPase-activating protein 17

Gene

Arhgap17

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere (By similarity). Participates in the Ca2+-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • actin filament organization Source: MGI
  • calcium ion regulated exocytosis Source: MGI
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 17
Alternative name(s):
Neuron-associated developmentally-regulated protein
Short name:
Nadrin
Rho-type GTPase-activating protein 17
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628767. Arhgap17.

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Rho GTPase-activating protein 17PRO_0000280464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei484 – 4841PhosphoserineBy similarity
Modified residuei575 – 5751PhosphoserineBy similarity
Modified residuei710 – 7101PhosphoserineBy similarity
Modified residuei712 – 7121PhosphoserineBy similarity
Modified residuei742 – 7421PhosphothreonineCombined sources
Modified residuei746 – 7461PhosphothreonineCombined sources
Modified residuei748 – 7481PhosphothreonineCombined sources
Modified residuei751 – 7511PhosphoserineBy similarity
Modified residuei752 – 7521PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99N37.
PRIDEiQ99N37.

PTM databases

iPTMnetiQ99N37.
PhosphoSiteiQ99N37.

Expressioni

Tissue specificityi

Highly expressed in brain; neuron-specific (at protein level). Isoform 2, isoform 3 and isoform 4 are predominantly expressed in neuronal tissues and correlate well with the differentiation of neurons, while isoform 1 is strongly expressed in embryonic brain.2 Publications

Developmental stagei

Becomes detectable at the second postnatal week in the cerebral cortex and hippocampus and at the third postnatal week in the cerebellum and olfactory bulb. The expression level is maximal during the third and fourth postnatal weeks and remains high during adulthood. Its expression is closely correlated with neuronal differentiation (at protein level).1 Publication

Interactioni

Subunit structurei

Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with SLC9A3R1, FNBP1, TRIP10, CAPZA (CAPZA1, CAPZA2 or CAPZA3), CAPZB, CD2AP and SH3KBP1/CIN85 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038504.

Structurei

3D structure databases

ProteinModelPortaliQ99N37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 246233BARPROSITE-ProRule annotationAdd
BLAST
Domaini252 – 442191Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi742 – 75514SH3-bindingSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi636 – 794159Pro-richAdd
BLAST
Compositional biasi674 – 70229Gln-richAdd
BLAST

Domaini

The BAR domain mediates the interaction with the coiled coil domain of AMOT, leading to its recruitment to tight junctions.By similarity

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
HOGENOMiHOG000179193.
HOVERGENiHBG000015.
InParanoidiQ99N37.
PhylomeDBiQ99N37.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00721. BAR. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99N37-1) [UniParc]FASTAAdd to basket
Also known as: Nadrin-126, Nadrin-E2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKQFNRMKQ LANQTVGRAE KTEVLSEDLL QIERRLDTVR SMCHHSHKRL
60 70 80 90 100
IACFQGQHGT DAERRHKKLP LTALAQNMQE ASAQLEESLL GKMLETCGDA
110 120 130 140 150
ENQLAFELSQ HEVFVEKEIM DPLYGIAEVE IPNIQKQRKQ LARLVLDWDS
160 170 180 190 200
VRARWNQAHK SSGTNFQGLP SKIDTLKEEM DEAGNKVEQC KDQLAADMYN
210 220 230 240 250
FMAKEGEYGK FFVTLLEAQA DYHRKALAVL EKALPEMRAH QDKWAEKPAF
260 270 280 290 300
GTPLEEHLKR SGREIALPIE ACVMLLLETG MKEEGLFRIG AGASKLKKLK
310 320 330 340 350
AALDCSTSHL DEFYSDPHAV AGALKSYLRE LPEPLMTFSL YEEWTQVASV
360 370 380 390 400
QDQDKKLQYL WTTCQKLPPQ NFVNFRYLIK FLAKLAQTSD VNKMTPSNIA
410 420 430 440 450
IVLGPNLLWA KQEGTLAEIA AATSVHVVAV IEPIIQHADW FFPGEVEFNV
460 470 480 490 500
SEAFVPLATP NSNHSSHTGN DSDSGTLERK RPASMAVMEG DLVKKESFGV
510 520 530 540 550
KLMDFQAHRR GGTLNRKHIS PAFQPPLPPT DGNALAPAGP ELPSQSSRAD
560 570 580 590 600
SNSVGGPVPS SSGILEQGLS PGDSSPPKPK DSVSAAAPVA GRNSNQITTV
610 620 630 640 650
PNQAQTGGNS HQLSVGTAHS AAGPSPHTLR RAVKKPAPAP PKPGNPPPGH
660 670 680 690 700
PGGQSSPGTG TSPKPSTRSP SPPQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ
710 720 730 740 750
QQTPGMRRCS SSLPPIQAPN HPPPQPPTQP RLGEQGPEPG PTPPQTPTPP
760 770 780 790 800
STPPPAKQNS SQSETTQLHG TLPRPRPVPK PRNRPSVPPP PNPPGTHMGD
810 820 830 840 850
GGLTPSVPTA SRIVTDTNSR VSESLRNIFP EIHSDLASKE VPGHILLDID

NDTESTAL
Length:858
Mass (Da):93,754
Last modified:June 1, 2001 - v1
Checksum:i4B3850A69090D88B
GO
Isoform 2 (identifier: Q99N37-2) [UniParc]FASTAAdd to basket
Also known as: Nadrin-104

The sequence of this isoform differs from the canonical sequence as follows:
     497-574: Missing.

Show »
Length:780
Mass (Da):85,824
Checksum:i180B75771C510246
GO
Isoform 3 (identifier: Q99N37-3) [UniParc]FASTAAdd to basket
Also known as: Nadrin-102

The sequence of this isoform differs from the canonical sequence as follows:
     497-574: Missing.
     817-858: TNSRVSESLRNIFPEIHSDLASKEVPGHILLDIDNDTESTAL → ALPGALTGGEGFQN

Show »
Length:752
Mass (Da):82,520
Checksum:iD9002F74E5BD1AE1
GO
Isoform 4 (identifier: Q99N37-4) [UniParc]FASTAAdd to basket
Also known as: Nadrin-116, Nadrin-E1

The sequence of this isoform differs from the canonical sequence as follows:
     817-858: TNSRVSESLRNIFPEIHSDLASKEVPGHILLDIDNDTESTAL → ALPGALTGGEGFQN

Show »
Length:830
Mass (Da):90,450
Checksum:iE5A8E4B25AD5F8F7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei497 – 57478Missing in isoform 2 and isoform 3. 2 PublicationsVSP_023693Add
BLAST
Alternative sequencei817 – 85842TNSRV…ESTAL → ALPGALTGGEGFQN in isoform 3 and isoform 4. 1 PublicationVSP_023694Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042827 mRNA. Translation: BAB12426.1.
AB060556 mRNA. Translation: BAB43864.1.
AB060557 mRNA. Translation: BAB43865.1.
AB080637 mRNA. Translation: BAB85655.1.
BC085736 mRNA. Translation: AAH85736.1.
RefSeqiNP_001257621.1. NM_001270692.1. [Q99N37-1]
NP_001257622.1. NM_001270693.1. [Q99N37-4]
NP_001257623.1. NM_001270694.1. [Q99N37-3]
NP_071580.1. NM_022244.2. [Q99N37-2]
UniGeneiRn.64581.

Genome annotation databases

GeneIDi63994.
KEGGirno:63994.
UCSCiRGD:628767. rat. [Q99N37-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042827 mRNA. Translation: BAB12426.1.
AB060556 mRNA. Translation: BAB43864.1.
AB060557 mRNA. Translation: BAB43865.1.
AB080637 mRNA. Translation: BAB85655.1.
BC085736 mRNA. Translation: AAH85736.1.
RefSeqiNP_001257621.1. NM_001270692.1. [Q99N37-1]
NP_001257622.1. NM_001270693.1. [Q99N37-4]
NP_001257623.1. NM_001270694.1. [Q99N37-3]
NP_071580.1. NM_022244.2. [Q99N37-2]
UniGeneiRn.64581.

3D structure databases

ProteinModelPortaliQ99N37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038504.

PTM databases

iPTMnetiQ99N37.
PhosphoSiteiQ99N37.

Proteomic databases

PaxDbiQ99N37.
PRIDEiQ99N37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi63994.
KEGGirno:63994.
UCSCiRGD:628767. rat. [Q99N37-1]

Organism-specific databases

CTDi55114.
RGDi628767. Arhgap17.

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
HOGENOMiHOG000179193.
HOVERGENiHBG000015.
InParanoidiQ99N37.
PhylomeDBiQ99N37.

Miscellaneous databases

PROiQ99N37.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00721. BAR. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG17_RAT
AccessioniPrimary (citable) accession number: Q99N37
Secondary accession number(s): Q8R506, Q99N38, Q9EQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.