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Protein

DNA fragmentation factor subunit beta

Gene

Dffb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Enzyme regulationi

Inhibited by DFFA (DFF45).

GO - Molecular functioni

  • deoxyribonuclease activity Source: RGD

GO - Biological processi

  • apoptotic DNA fragmentation Source: RGD
  • brain development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
DNA fragmentation factor subunit beta (EC:3.-.-.-)
Alternative name(s):
Caspase-activated deoxyribonuclease
Short name:
CAD
Short name:
Caspase-activated DNase
DNA fragmentation factor 40 kDa subunit
Short name:
DFF-40
Gene namesi
Name:Dffb
Synonyms:Cad
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620335. Dffb.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear chromatin Source: GO_Central
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447151 – 349DNA fragmentation factor subunit betaAdd BLAST349

Proteomic databases

PaxDbiQ99N34.
PRIDEiQ99N34.

PTM databases

iPTMnetiQ99N34.
PhosphoSitePlusiQ99N34.

Expressioni

Gene expression databases

BgeeiENSRNOG00000025030.
GenevisibleiQ99N34. RN.

Interactioni

Subunit structurei

Heterodimer of DFFA and DFFB. Interacts with HIST1H1A (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Nfkb1Q633692EBI-8498730,EBI-8498561

Protein-protein interaction databases

IntActiQ99N34. 1 interactor.
MINTiMINT-2740288.
STRINGi10116.ENSRNOP00000034722.

Structurei

3D structure databases

ProteinModelPortaliQ99N34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 83CIDE-NPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Contains 1 CIDE-N domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFTE. Eukaryota.
ENOG41102TS. LUCA.
HOGENOMiHOG000006502.
HOVERGENiHBG003828.
InParanoidiQ99N34.
KOiK02311.
OrthoDBiEOG091G0BE7.
PhylomeDBiQ99N34.
TreeFamiTF102022.

Family and domain databases

InterProiIPR015311. Apoptosis_DFF40.
IPR003508. CIDE-N_dom.
[Graphical view]
PfamiPF02017. CIDE-N. 1 hit.
PF09230. DFF40. 1 hit.
[Graphical view]
SMARTiSM00266. CAD. 1 hit.
[Graphical view]
PROSITEiPS51135. CIDE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99N34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAVLPQPKC VKLRALHSSC KFGVAARSCQ ELLRKGCIRF QLPVPGSRLC
60 70 80 90 100
MYEDGTEVTD DCFPSLPNDS ELLLLTAGET WHGYVSDITR LLSVFNEPHA
110 120 130 140 150
GVIQAARQLL SDEQAPLRQK LLADLLHHVS QNITAETREQ DPSWFEGLES
160 170 180 190 200
RFRNKSGYLR YSCESRIRGY LREVSAYISM VDAAAREEYL RVLSSMCHKL
210 220 230 240 250
KSVQYNGSYF DRGAEASSRL CTPEGWFSCQ GPFDLESCLS KHSINPYGNR
260 270 280 290 300
ESRILFSTWN LDHIIEKKRT VVPTLAEAIQ DGREVNWEYF YSLLFTAENL
310 320 330 340
KLVHIACHKK TTHKLQCDRS RIYRPQTGSR RKQPPRKQPP RKRPPRKRQ
Length:349
Mass (Da):40,097
Last modified:June 1, 2001 - v1
Checksum:iF125972A110FE398
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136598 mRNA. Translation: AAK16646.1.
RefSeqiNP_445814.1. NM_053362.1.
UniGeneiRn.67077.

Genome annotation databases

GeneIDi84359.
KEGGirno:84359.
UCSCiRGD:620335. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136598 mRNA. Translation: AAK16646.1.
RefSeqiNP_445814.1. NM_053362.1.
UniGeneiRn.67077.

3D structure databases

ProteinModelPortaliQ99N34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99N34. 1 interactor.
MINTiMINT-2740288.
STRINGi10116.ENSRNOP00000034722.

PTM databases

iPTMnetiQ99N34.
PhosphoSitePlusiQ99N34.

Proteomic databases

PaxDbiQ99N34.
PRIDEiQ99N34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84359.
KEGGirno:84359.
UCSCiRGD:620335. rat.

Organism-specific databases

CTDi1677.
RGDi620335. Dffb.

Phylogenomic databases

eggNOGiENOG410IFTE. Eukaryota.
ENOG41102TS. LUCA.
HOGENOMiHOG000006502.
HOVERGENiHBG003828.
InParanoidiQ99N34.
KOiK02311.
OrthoDBiEOG091G0BE7.
PhylomeDBiQ99N34.
TreeFamiTF102022.

Miscellaneous databases

PROiQ99N34.

Gene expression databases

BgeeiENSRNOG00000025030.
GenevisibleiQ99N34. RN.

Family and domain databases

InterProiIPR015311. Apoptosis_DFF40.
IPR003508. CIDE-N_dom.
[Graphical view]
PfamiPF02017. CIDE-N. 1 hit.
PF09230. DFF40. 1 hit.
[Graphical view]
SMARTiSM00266. CAD. 1 hit.
[Graphical view]
PROSITEiPS51135. CIDE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDFFB_RAT
AccessioniPrimary (citable) accession number: Q99N34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.