Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-klotho

Gene

Klb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.2 Publications

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • fibroblast growth factor receptor signaling pathway Source: MGI
  • glycosyl compound metabolic process Source: GO_Central
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-109704 PI3K Cascade
R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-1307965 betaKlotho-mediated ligand binding
R-MMU-5654228 Phospholipase C-mediated cascade, FGFR4
R-MMU-5654693 FRS-mediated FGFR1 signaling
R-MMU-5654700 FRS-mediated FGFR2 signaling
R-MMU-5654706 FRS-mediated FGFR3 signaling
R-MMU-5654712 FRS-mediated FGFR4 signaling
R-MMU-5654719 SHC-mediated cascade:FGFR4
R-MMU-5654720 PI-3K cascade:FGFR4
R-MMU-5654733 Negative regulation of FGFR4 signaling
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-klotho
Short name:
BKL
Short name:
BetaKlotho
Alternative name(s):
Klotho beta-like protein
Gene namesi
Name:Klb
Synonyms:Betakl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1932466 Klb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 994ExtracellularSequence analysisAdd BLAST994
Transmembranei995 – 1015HelicalSequence analysisAdd BLAST21
Topological domaini1016 – 1043CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, but have an altered bile metabolism: they have increased CYP7A1 levels, secrete more bile acids and are resistant to cholesterol gallstone formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000639061 – 1043Beta-klothoAdd BLAST1043

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi211N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi552N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi609N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi700N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi704N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi837N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ99N32
PRIDEiQ99N32

PTM databases

iPTMnetiQ99N32
PhosphoSitePlusiQ99N32

Expressioni

Tissue specificityi

Present in liver, muscle and white adipose tissue, but not in kidney (at protein level). Expressed in liver and pancreas, and at lower levels in skin, stomach, skeletal muscle, small intestine and lung.3 Publications

Developmental stagei

Expression starts at E10.5 in hepatocytes. Expressed in the acinar cells of the pancreas and cervical brown adipose tissue at E15.5. Expressed in white adipose tissue at E19.5. Up-regulated during preadipocyte differentiation into adipocytes (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000029195
CleanExiMM_KLB
ExpressionAtlasiQ99N32 baseline and differential
GenevisibleiQ99N32 MM

Interactioni

Subunit structurei

Interacts with FGF19; this interaction is direct. Interacts (via C-terminus) with FGF21; this interaction is direct (By similarity). Interacts with FGFR1 and FGFR4.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60918N
IntActiQ99N32, 2 interactors
STRINGi10090.ENSMUSP00000031096

Structurei

3D structure databases

ProteinModelPortaliQ99N32
SMRiQ99N32
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 506Glycosyl hydrolase-1 1Add BLAST430
Regioni515 – 965Glycosyl hydrolase-1 2Add BLAST451

Domaini

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
GeneTreeiENSGT00550000074452
HOGENOMiHOG000060126
HOVERGENiHBG081856
InParanoidiQ99N32
KOiK22404
OMAiMLARMKV
OrthoDBiEOG091G0035
PhylomeDBiQ99N32
TreeFamiTF314803

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 6 hits
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 3 hits
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 2 hits
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99N32-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTGCAAGSP GNEWIFFSSD ERNTRSRKTM SNRALQRSAV LSAFVLLRAV
60 70 80 90 100
TGFSGDGKAI WDKKQYVSPV NPSQLFLYDT FPKNFSWGVG TGAFQVEGSW
110 120 130 140 150
KTDGRGPSIW DRYVYSHLRG VNGTDRSTDS YIFLEKDLLA LDFLGVSFYQ
160 170 180 190 200
FSISWPRLFP NGTVAAVNAQ GLRYYRALLD SLVLRNIEPI VTLYHWDLPL
210 220 230 240 250
TLQEEYGGWK NATMIDLFND YATYCFQTFG DRVKYWITIH NPYLVAWHGF
260 270 280 290 300
GTGMHAPGEK GNLTAVYTVG HNLIKAHSKV WHNYDKNFRP HQKGWLSITL
310 320 330 340 350
GSHWIEPNRT DNMEDVINCQ HSMSSVLGWF ANPIHGDGDY PEFMKTGAMI
360 370 380 390 400
PEFSEAEKEE VRGTADFFAF SFGPNNFRPS NTVVKMGQNV SLNLRQVLNW
410 420 430 440 450
IKLEYDDPQI LISENGWFTD SYIKTEDTTA IYMMKNFLNQ VLQAIKFDEI
460 470 480 490 500
RVFGYTAWTL LDGFEWQDAY TTRRGLFYVD FNSEQKERKP KSSAHYYKQI
510 520 530 540 550
IQDNGFPLKE STPDMKGRFP CDFSWGVTES VLKPEFTVSS PQFTDPHLYV
560 570 580 590 600
WNVTGNRLLY RVEGVRLKTR PSQCTDYVSI KKRVEMLAKM KVTHYQFALD
610 620 630 640 650
WTSILPTGNL SKVNRQVLRY YRCVVSEGLK LGVFPMVTLY HPTHSHLGLP
660 670 680 690 700
LPLLSSGGWL NMNTAKAFQD YAELCFRELG DLVKLWITIN EPNRLSDMYN
710 720 730 740 750
RTSNDTYRAA HNLMIAHAQV WHLYDRQYRP VQHGAVSLSL HCDWAEPANP
760 770 780 790 800
FVDSHWKAAE RFLQFEIAWF ADPLFKTGDY PSVMKEYIAS KNQRGLSSSV
810 820 830 840 850
LPRFTAKESR LVKGTVDFYA LNHFTTRFVI HKQLNTNRSV ADRDVQFLQD
860 870 880 890 900
ITRLSSPSRL AVTPWGVRKL LAWIRRNYRD RDIYITANGI DDLALEDDQI
910 920 930 940 950
RKYYLEKYVQ EALKAYLIDK VKIKGYYAFK LTEEKSKPRF GFFTSDFRAK
960 970 980 990 1000
SSVQFYSKLI SSSGLPAENR SPACGQPAED TDCTICSFLV EKKPLIFFGC
1010 1020 1030 1040
CFISTLAVLL SITVFHHQKR RKFQKARNLQ NIPLKKGHSR VFS
Length:1,043
Mass (Da):120,189
Last modified:June 1, 2001 - v1
Checksum:i4CBE6735F4112D5E
GO
Isoform 2 (identifier: Q99N32-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-295: AHSKVWHNYDKNFRPHQKGW → VLYSWLLTRASELGGGVLGG
     296-1043: Missing.

Show »
Length:295
Mass (Da):33,142
Checksum:i0B1C8C4453E11D19
GO

Sequence cautioni

The sequence AAL01648 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015830276 – 295AHSKV…HQKGW → VLYSWLLTRASELGGGVLGG in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_015831296 – 1043Missing in isoform 2. 1 PublicationAdd BLAST748

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178429 mRNA Translation: AAL01648.1 Different initiation.
AF165170 mRNA Translation: AAK28704.1
AF165171 mRNA Translation: AAK28705.1
BC138008 mRNA Translation: AAI38009.1
BC138010 mRNA Translation: AAI38011.1
CCDSiCCDS19306.1 [Q99N32-1]
RefSeqiNP_112457.1, NM_031180.2 [Q99N32-1]
UniGeneiMm.45274

Genome annotation databases

EnsembliENSMUST00000031096; ENSMUSP00000031096; ENSMUSG00000029195 [Q99N32-1]
GeneIDi83379
KEGGimmu:83379
UCSCiuc008xnn.1 mouse [Q99N32-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKLOTB_MOUSE
AccessioniPrimary (citable) accession number: Q99N32
Secondary accession number(s): B2RQN8, Q920J2, Q99N31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health