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Q99N27 (SNX1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sorting nexin-1
Gene names
Name:Snx1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) By similarity.

Subunit structure

Self-assembles into a complex of approximately 300 kDa. Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts with SNX6 By similarity. Interacts with HGS. Ref.1

Subcellular location

Endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) By similarity.

Miscellaneous

Binds phosphatidylinositol 3-phosphate (PtdIns-3P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2) in liposome-based assays. Can bind PtdIns(3,4,5)P3 in protein:lipid overlay assays, but not in liposome-based assays By similarity.

Sequence similarities

Belongs to the sorting nexin family.

Contains 1 BAR domain.

Contains 1 PX (phox homology) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 522522Sorting nexin-1
PRO_0000213837

Regions

Domain143 – 272130PX
Domain302 – 522221BAR

Sites

Binding site1861Phosphatidylinositol 3-phosphate By similarity
Binding site1881Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site2141Phosphatidylinositol 3-phosphate By similarity
Binding site2381Phosphatidylinositol 3-phosphate By similarity

Amino acid modifications

Modified residue321Phosphoserine By similarity
Modified residue391Phosphoserine By similarity
Modified residue1881Phosphoserine By similarity
Modified residue2371N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99N27 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: F0F05664087E4D24

FASTA52259,044
        10         20         30         40         50         60 
MASGGGGCSA SERLPPPFPG MDPESEGAAG GSEPEAGDSD TEGEDIFTGA AAASKPQSPK 

        70         80         90        100        110        120 
KTTSLFPIKN GSKENGIHEE QDQEPQDLFA DATVELSLDS TQNNQKTMPG KTLIPHPTQE 

       130        140        150        160        170        180 
ATNSPKPQPS YEELEEEEQE DQFDLTVGIT DPEKIGDGMN AYVAYKVTTQ TSLPMFRSRQ 

       190        200        210        220        230        240 
FAVKRRFSDF LGLYEKLSEK HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA 

       250        260        270        280        290        300 
ALERYLQRIV NHPTMLQDPD VREFLEKEEL PRAVGTQALS GAGLLKMFNK ATDAVSKMTI 

       310        320        330        340        350        360 
KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTALFAKS LAMLGSSEDN 

       370        380        390        400        410        420 
TALSRALSQL AEVEEKIEQL HQEQANNDFF LLAELLSDYI RLLAIVRAAF DQRMKTWQRW 

       430        440        450        460        470        480 
QDAQATLQKK RESEARLLWA NKPDKLQQAK DEITEWESRV TQYERDFERI STVVRKEVTR 

       490        500        510        520 
FEKEKSKDFK NHVIKYLETL LHSQQQLAKY WEAFLPEARA IS 

« Hide

References

« Hide 'large scale' references
[1]"Hrs interacts with sorting nexin 1 and regulates degradation of epidermal growth factor receptor."
Chin L.-S., Raynor M.C., Wei X., Chen H.-Q., Li L.
J. Biol. Chem. 276:7069-7078(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH HGS.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF218916 mRNA. Translation: AAG59616.1.
BC092201 mRNA. Translation: AAH92201.1.
RefSeqNP_445863.1. NM_053411.1.
UniGeneRn.144614.

3D structure databases

ProteinModelPortalQ99N27.
SMRQ99N27. Positions 142-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ99N27. 1 interaction.

PTM databases

PhosphoSiteQ99N27.

Proteomic databases

PaxDbQ99N27.
PRIDEQ99N27.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000022960; ENSRNOP00000022960; ENSRNOG00000017029.
GeneID84471.
KEGGrno:84471.
UCSCRGD:68935. rat.

Organism-specific databases

CTD6642.
RGD68935. Snx1.

Phylogenomic databases

eggNOGCOG5391.
GeneTreeENSGT00740000114933.
HOGENOMHOG000293327.
HOVERGENHBG000618.
InParanoidQ99N27.
KOK17917.
OMAPQPTYEE.
OrthoDBEOG7HHWSB.
PhylomeDBQ99N27.
TreeFamTF313698.

Gene expression databases

GenevestigatorQ99N27.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR001683. Phox.
IPR028660. SNX1.
IPR005329. Sorting_nexin_N.
IPR015404. Vps5_C.
[Graphical view]
PANTHERPTHR10555:SF30. PTHR10555:SF30. 1 hit.
PfamPF00787. PX. 1 hit.
PF03700. Sorting_nexin. 1 hit.
PF09325. Vps5. 1 hit.
[Graphical view]
SMARTSM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF64268. SSF64268. 1 hit.
PROSITEPS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio616956.
PROQ99N27.

Entry information

Entry nameSNX1_RAT
AccessionPrimary (citable) accession number: Q99N27
Secondary accession number(s): Q56A25
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families