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Q99N16

- CP4F3_MOUSE

UniProt

Q99N16 - CP4F3_MOUSE

Protein

Leukotriene-B(4) omega-hydroxylase 2

Gene

Cyp4f3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.By similarity1 Publication

    Catalytic activityi

    (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O.By similarity

    Cofactori

    Heme group.By similarity

    Enzyme regulationi

    Inhibited by carbon monoxide (CO).By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi468 – 4681Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. alpha-tocopherol omega-hydroxylase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. leukotriene-B4 20-monooxygenase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
    REACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    REACT_216259. Miscellaneous substrates.
    UniPathwayiUPA00883.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leukotriene-B(4) omega-hydroxylase 2 (EC:1.14.13.30)
    Alternative name(s):
    CYPIVF3
    Cytochrome P450 4F3
    Cytochrome P450-LTB-omega
    Leukotriene-B(4) 20-monooxygenase 2
    Gene namesi
    Name:Cyp4f3By similarity
    Synonyms:Cyp4f18Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1919304. Cyp4f18.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Leukotriene-B(4) omega-hydroxylase 2PRO_0000238924Add
    BLAST

    Proteomic databases

    PaxDbiQ99N16.
    PRIDEiQ99N16.

    PTM databases

    PhosphoSiteiQ99N16.

    Expressioni

    Tissue specificityi

    Highest level in polymorphonuclear leukocytes and dendritic cells. Detectable in lymph nodes, spleen, bone marrow and peripheral blood. Highly expressed in ovary. Very low level in liver, kidney, and smooth muscle.1 Publication

    Inductioni

    Up-regulated in myeloid dendritic cells upon induction with LPS in vitro. Down-regulated upon migration of induced cells to the lymph node.1 Publication

    Gene expression databases

    BgeeiQ99N16.
    GenevestigatoriQ99N16.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000003574.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99N16.
    SMRiQ99N16. Positions 96-524.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Sequence Analysis

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00730000110562.
    HOGENOMiHOG000233833.
    HOVERGENiHBG000182.
    InParanoidiQ99N16.
    KOiK17726.
    OMAiVISRISI.
    OrthoDBiEOG7CNZFK.
    TreeFamiTF105088.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99N16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQLSMSWMG LGHTAASPWL LLLLAGASCL LAYILTPIYG VFENSLRLRC    50
    FPQPPKRNWI LGHLGLIQSS EEGLLYIQSL VRTFRDACCW WVGPLHPVIR 100
    IFHPAFIKPV VLAPALVAPK DTVFYRFLKP WLGDGLLMST GDKWSRHRRM 150
    LTPAFHFNIL KPYVKVFNDS TNIMHAKWQR LASKGSAYLN MFEHISLMTL 200
    DSLQKCVFSF DSNCQEKPSE YITAILELST LVARRHQRLL LHVDLFYYLT 250
    HDGMRFRKAC RLVHDFTDAV IRERRRTLLD QGGVDVLKAK AKAKTLDFID 300
    VLLLSKDEHG KALSDEDIRA EADTFMFGGH DTTASGLSWI LYNLARHPEY 350
    QERCRQEVRE LLRDREPEEI EWDDLAQLPF LTMCIKESLR LHPPVTAISR 400
    CCTQDIVLPD GRVIPKGVIS RISIFGTHHN PAVWPDPEVY DPFRFDADNV 450
    KGRSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR VLPDDKEPRR 500
    KPELILRAEG GLWLKVEPLS AGAQ 524
    Length:524
    Mass (Da):59,843
    Last modified:July 27, 2011 - v2
    Checksum:iE97FACEB00CFD7CB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61M → L in AAK15013. 1 PublicationCurated
    Sequence conflicti6 – 61M → L in AAH13494. (PubMed:15489334)Curated
    Sequence conflicti37 – 371P → Q in AAK15013. 1 PublicationCurated
    Sequence conflicti37 – 371P → Q in AAH13494. (PubMed:15489334)Curated
    Sequence conflicti142 – 1421D → G in BAB25315. (PubMed:16141072)Curated
    Sequence conflicti230 – 2301T → A in AAK15013. 1 PublicationCurated
    Sequence conflicti230 – 2301T → A in AAH13494. (PubMed:15489334)Curated
    Sequence conflicti284 – 2841V → D in AAK15013. 1 PublicationCurated
    Sequence conflicti284 – 2841V → D in AAH13494. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF233647 mRNA. Translation: AAK15013.1.
    AK007863 mRNA. Translation: BAB25315.1.
    AC162522 Genomic DNA. No translation available.
    BC013494 mRNA. Translation: AAH13494.1.
    CCDSiCCDS22406.1.
    RefSeqiNP_077764.2. NM_024444.2.
    XP_006509811.1. XM_006509748.1.
    UniGeneiMm.160020.

    Genome annotation databases

    EnsembliENSMUST00000003574; ENSMUSP00000003574; ENSMUSG00000003484.
    GeneIDi72054.
    KEGGimmu:72054.
    UCSCiuc009mfe.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF233647 mRNA. Translation: AAK15013.1 .
    AK007863 mRNA. Translation: BAB25315.1 .
    AC162522 Genomic DNA. No translation available.
    BC013494 mRNA. Translation: AAH13494.1 .
    CCDSi CCDS22406.1.
    RefSeqi NP_077764.2. NM_024444.2.
    XP_006509811.1. XM_006509748.1.
    UniGenei Mm.160020.

    3D structure databases

    ProteinModelPortali Q99N16.
    SMRi Q99N16. Positions 96-524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000003574.

    PTM databases

    PhosphoSitei Q99N16.

    Proteomic databases

    PaxDbi Q99N16.
    PRIDEi Q99N16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003574 ; ENSMUSP00000003574 ; ENSMUSG00000003484 .
    GeneIDi 72054.
    KEGGi mmu:72054.
    UCSCi uc009mfe.2. mouse.

    Organism-specific databases

    CTDi 72054.
    MGIi MGI:1919304. Cyp4f18.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00730000110562.
    HOGENOMi HOG000233833.
    HOVERGENi HBG000182.
    InParanoidi Q99N16.
    KOi K17726.
    OMAi VISRISI.
    OrthoDBi EOG7CNZFK.
    TreeFami TF105088.

    Enzyme and pathway databases

    UniPathwayi UPA00883 .
    Reactomei REACT_196572. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
    REACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    REACT_216259. Miscellaneous substrates.

    Miscellaneous databases

    NextBioi 335324.
    PROi Q99N16.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q99N16.
    Genevestigatori Q99N16.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Protein expression and catalytic activity assessment of mouse 4F clones."
      Antonovic L., Kawashima H., Strobel H.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: KidneyImported.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported.
      Tissue: PancreasImported.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/NImported.
      Tissue: ColonImported.
    5. "Cytochrome P-450 4F18 is the leukotriene B4 omega-1/omega-2 hydroxylase in mouse polymorphonuclear leukocytes: identification as the functional orthologue of human polymorphonuclear leukocyte CYP4F3A in the down-regulation of responses to LTB4."
      Christmas P., Tolentino K., Primo V., Berry K.Z., Murphy R.C., Chen M., Lee D.M., Soberman R.J.
      J. Biol. Chem. 281:7189-7196(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiCP4F3_MOUSE
    AccessioniPrimary (citable) accession number: Q99N16
    Secondary accession number(s): E9QLZ3, Q9D8N4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3