Reviewed,
UniProtKB/Swiss-Prot Q99N16 (CP4F3_MOUSE)
Last modified
February 9, 2010.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Leukotriene-B(4) omega-hydroxylase 2 EC=1.14.13.30 Alternative name(s): Cytochrome P450 4F3 CYPIVF3 Leukotriene-B(4) 20-monooxygenase 2 Cytochrome P450-LTB-omega | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 524 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes. Ref.4 UniProtKB Q08477 |
| Catalytic activity | (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O. UniProtKB Q08477 |
| Cofactor | Heme group By similarity. |
| Enzyme regulation | Inhibited by carbon monoxide (CO) By similarity. UniProtKB Q08477 |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Microsome membrane; Single-pass membrane protein By similarity. |
| Tissue specificity | Highest level in polymorphonuclear leukocytes and dendritic cells. Detectable in lymph nodes, spleen, bone marrow and peripheral blood. Highly expressed in ovary. Very low level in liver, kidney, and smooth muscle. Ref.4 |
| Induction | Up-regulated in myeloid dendritic cells upon induction with LPS in vitro. Down-regulated upon migration of induced cells to the lymph node. Ref.4 |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane Microsome |
| Domain | Transmembrane |
| Ligand | Heme Iron Metal-binding NADP |
| Molecular function | Monooxygenase Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell microsomeInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | electron carrier activity Inferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro leukotriene-B4 20-monooxygenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 524 | 524 | Leukotriene-B(4) omega-hydroxylase 2 | PRO_0000238924 | |||||
Regions | |||||||||
| Transmembrane | 15 – 35 | 21 | Potential | ||||||
Sites | |||||||||
| Metal binding | 468 | 1 | Iron (heme axial ligand) By similarity UniProtKB P33274 | ||||||
Experimental info | |||||||||
| Sequence conflict | 6 | 1 | L → M in BAB25315. Ref.2 | ||||||
| Sequence conflict | 37 | 1 | Q → P in BAB25315. Ref.2 | ||||||
| Sequence conflict | 142 | 1 | D → G in BAB25315. Ref.2 | ||||||
| Sequence conflict | 230 | 1 | A → T in BAB25315. Ref.2 | ||||||
| Sequence conflict | 284 | 1 | D → V in BAB25315. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Protein expression and catalytic activity assessment of mouse 4F clones." Antonovic L., Kawashima H., Strobel H. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Pancreas. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon. |
| [4] | "Cytochrome P-450 4F18 is the leukotriene B4 omega-1/omega-2 hydroxylase in mouse polymorphonuclear leukocytes: identification as the functional orthologue of human polymorphonuclear leukocyte CYP4F3A in the down-regulation of responses to LTB4." Christmas P., Tolentino K., Primo V., Berry K.Z., Murphy R.C., Chen M., Lee D.M., Soberman R.J. J. Biol. Chem. 281:7189-7196(2006) [PubMed: 16380383] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF233647 mRNA. Translation: AAK15013.1. AK007863 mRNA. Translation: BAB25315.1. BC013494 mRNA. Translation: AAH13494.1. |
| IPI | IPI00117991. |
| RefSeq | NP_077764.2. |
| UniGene | Mm.160020 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q99N16. |
Proteomic databases | |
| PRIDE | Q99N16. |
Genome annotation databases | |
| Ensembl | ENSMUST00000003574; ENSMUSP00000003574; ENSMUSG00000003484; Mus musculus. [Genome view] |
| GeneID | 72054. |
| KEGG | mmu:72054. |
| UCSC | uc009mfe.1. mouse. |
Organism-specific databases | |
| CTD | 72054. |
| MGI | MGI:1919304. Cyp4f18. |
Phylogenomic databases | |
| eggNOG | maNOG13304. |
| HOGENOM | HBG505954. |
| HOVERGEN | Q99N16. |
| InParanoid | Q99N16. |
| PhylomeDB | Q99N16. |
Enzyme and pathway databases | |
| BRENDA | 1.14.13.30. 244. |
Gene expression databases | |
| ArrayExpress | Q99N16. |
| Bgee | Q99N16. |
| Genevestigator | Q99N16. |
| GermOnline | ENSMUSG00000003484. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| PANTHER | PTHR19383. Cyt_P450. 1 hit. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR00385. P450. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 335324. |
| SOURCE | Search... |
Entry information
| Entry name | CP4F3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q99N16 Secondary accession number(s): Q9D8N4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


