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Protein

Unconventional myosin-VIIb

Gene

Myo7b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. May link the complex to the actin core bundle of microvilli.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-VIIb
Gene namesi
Name:Myo7b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:107709. Myo7b.

Subcellular locationi

GO - Cellular componenti

  • apical cytoplasm Source: UniProtKB
  • brush border Source: UniProtKB
  • extracellular exosome Source: MGI
  • microvillus Source: UniProtKB
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003290471 – 2113Unconventional myosin-VIIbAdd BLAST2113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei904PhosphoserineCombined sources1
Modified residuei1339PhosphothreonineCombined sources1
Modified residuei1368PhosphoserineCombined sources1
Modified residuei1642PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99MZ6.
PaxDbiQ99MZ6.
PeptideAtlasiQ99MZ6.
PRIDEiQ99MZ6.

PTM databases

iPTMnetiQ99MZ6.
PhosphoSitePlusiQ99MZ6.

Expressioni

Tissue specificityi

Expressed primarily in kidney and intestine. Detected in proximal tubule cells of the kidney and enterocytes of the intestine, specifically the distal tips of apical microvilli on these transporting epithelial cells (at protein level).2 Publications

Developmental stagei

Detected in intestinal enterocytes at embryonic day 17.1 Publication

Gene expression databases

BgeeiENSMUSG00000024388.
GenevisibleiQ99MZ6. MM.

Interactioni

Subunit structurei

Part of the IMAC/intermicrovillar adhesion complex/intermicrovillar tip-link complex composed of ANKS4B, MYO7B, USH1C, CDHR2 and CDHR5. Interacts with CDHR2. Interacts with CDHR5. Interacts with USH1C (By similarity). Interacts with ANKS4B; requires initial interaction with USH1C (Probable).By similarity1 Publication

Protein-protein interaction databases

IntActiQ99MZ6. 3 interactors.
MINTiMINT-4124477.
STRINGi10090.ENSMUSP00000118046.

Structurei

Secondary structure

12113
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi973 – 980Combined sources8
Helixi1006 – 1020Combined sources15
Helixi1086 – 1099Combined sources14
Helixi1103 – 1115Combined sources13
Helixi1121 – 1137Combined sources17
Turni1142 – 1144Combined sources3
Helixi1145 – 1153Combined sources9
Turni1157 – 1159Combined sources3
Helixi1160 – 1173Combined sources14
Helixi1182 – 1190Combined sources9
Beta strandi1194 – 1200Combined sources7
Beta strandi1205 – 1210Combined sources6
Helixi1216 – 1227Combined sources12
Beta strandi1235 – 1241Combined sources7
Beta strandi1244 – 1247Combined sources4
Turni1249 – 1252Combined sources4
Helixi1255 – 1269Combined sources15
Turni1273 – 1275Combined sources3
Beta strandi1278 – 1284Combined sources7
Helixi1297 – 1313Combined sources17
Beta strandi1314 – 1316Combined sources3
Helixi1321 – 1335Combined sources15
Helixi1342 – 1352Combined sources11
Helixi1355 – 1358Combined sources4
Helixi1363 – 1377Combined sources15
Turni1378 – 1380Combined sources3
Helixi1386 – 1400Combined sources15
Turni1402 – 1405Combined sources4
Beta strandi1406 – 1415Combined sources10
Beta strandi1422 – 1428Combined sources7
Beta strandi1433 – 1436Combined sources4
Beta strandi1442 – 1446Combined sources5
Helixi1448 – 1450Combined sources3
Beta strandi1451 – 1456Combined sources6
Beta strandi1465 – 1472Combined sources8
Beta strandi1474 – 1478Combined sources5
Helixi1482 – 1499Combined sources18
Beta strandi1502 – 1505Combined sources4
Beta strandi1525 – 1527Combined sources3
Beta strandi1537 – 1545Combined sources9
Turni1546 – 1548Combined sources3
Beta strandi1551 – 1555Combined sources5
Helixi1556 – 1558Combined sources3
Beta strandi1559 – 1561Combined sources3
Helixi1570 – 1575Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F3YX-ray3.41A962-1578[»]
ProteinModelPortaliQ99MZ6.
SMRiQ99MZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 760Myosin motorAdd BLAST696
Domaini745 – 765IQ 1PROSITE-ProRule annotationAdd BLAST21
Domaini763 – 792IQ 2PROSITE-ProRule annotationAdd BLAST30
Domaini786 – 815IQ 3PROSITE-ProRule annotationAdd BLAST30
Domaini809 – 838IQ 4PROSITE-ProRule annotationAdd BLAST30
Domaini832 – 861IQ 5PROSITE-ProRule annotationAdd BLAST30
Domaini855 – 884IQ 6PROSITE-ProRule annotationAdd BLAST30
Domaini989 – 1189MyTH4 1PROSITE-ProRule annotationAdd BLAST201
Domaini1194 – 1503FERM 1PROSITE-ProRule annotationAdd BLAST310
Domaini1498 – 1564SH3 1PROSITE-ProRule annotationAdd BLAST67
Domaini1606 – 1670SH3 2PROSITE-ProRule annotationAdd BLAST65
Domaini1641 – 1790MyTH4 2PROSITE-ProRule annotationAdd BLAST150
Domaini1790 – 1896MyTH4 3PROSITE-ProRule annotationAdd BLAST107
Domaini1796 – 2099FERM 2PROSITE-ProRule annotationAdd BLAST304

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni962 – 1578Mediates interaction with ANKS4B1 PublicationAdd BLAST617
Regioni1497 – 2113Mediates interaction with CDHR2, CDHR5 and USH1CBy similarityAdd BLAST617

Sequence similaritiesi

Contains 2 FERM domains.PROSITE-ProRule annotation
Contains 6 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 3 MyTH4 domains.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129687.
HOGENOMiHOG000007836.
HOVERGENiHBG052557.
InParanoidiQ99MZ6.
KOiK10359.
OMAiKEFFTPW.
OrthoDBiEOG091G00BB.
PhylomeDBiQ99MZ6.
TreeFamiTF335306.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR000857. MyTH4_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001452. SH3_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00784. MyTH4. 2 hits.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00295. B41. 2 hits.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00139. MyTH4. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50057. FERM_3. 2 hits.
PS50096. IQ. 4 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51016. MYTH4. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99MZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFRLGDHV WLDPPSSSKT GVAIGGIVKE TKLGKTLIED DEGKEHWVHA
60 70 80 90 100
EDLSTLRPMH PNSAQGVDDM IRLGDLNEAG VVHNLLIRYQ QHKIYTYTGS
110 120 130 140 150
ILVAVNPFQM LPLYTLEQVQ IYYSRHMGEL PPHIFAIANS CYFNMKKNKR
160 170 180 190 200
DQCCIISGES GAGKTETTKL ILQFLATVSG QHSWIEQQVL EANPILEAFG
210 220 230 240 250
NAKTIRNDNS SRFGKYIDIH FNSSGVIEGA SIEHFLLEKS RVCRQAPEER
260 270 280 290 300
NYHIFYCMLM GMSPEEKQML SLGMPSEYHY LTMGSCTSSE GLSDAKDYAH
310 320 330 340 350
VRSAMKILQF SDSENWDISK LLAAILHLGN VGFMAAVFEN LDSSDVMETP
360 370 380 390 400
AFPLAMKLLE VQHQALRDCL IKHTIPVLGE FVSRPVNIAQ ATDRRDAFVK
410 420 430 440 450
GIYGRLFQWI VKKINAAIFT PQAQDPQNVR RAIGLLDIFG FENFQNNSFE
460 470 480 490 500
QLCINFANEH LQQFFVKHVF TMEQEEYLSE NITWNYIHYT DNQPILDMLA
510 520 530 540 550
LKPMSIISLL DEESRFPQGT DVTMLQKLNS IHANNKSFLS PRSIHDTRFG
560 570 580 590 600
IAHFAGDVYY QAEGFLEKNR DVLSTDILIL IHSSKNKFLK EIFNVDSSQT
610 620 630 640 650
KLGHGTICQV KAGSQLFKSS DSIKRPVTLA SQFKQSLDQL MRILTNCQPY
660 670 680 690 700
FVRCIKPNEY KKPLLFDREL CIQQLRYSGM METVHIRKSG FPIRYTFDEF
710 720 730 740 750
SQRFRVLLPS PERMQFQNKP RQMTLHIADL CLGTDKDWKV GKTKIFLKDH
760 770 780 790 800
QDTVLEIRRS QALDGAAIRI QRVLRGHKYR KEFLRQRRAA VTLQAGWRGY
810 820 830 840 850
SQRKNFKLIL VGFERLQAIA RSHLLMRQFQ AMRQRIVQLQ ARCRGYLVRQ
860 870 880 890 900
QVQAKRRAVV IIQAHARGMV VRKSYWQQKS TGPQVILAKE PKAQVAVHER
910 920 930 940 950
KRKSIYDTVT DTAMVEKVFG FLPAMIGGQE GPAPTRFEDL EVKTQKLHEV
960 970 980 990 1000
DLDTVPMMAM PEEEVDSLAE YTFPKFAVTY FQKSASHTHI QKPLRYPLLY
1010 1020 1030 1040 1050
HENDTDHSAA LDVWIIILRF MGDLPEPVVY GRNSLTGSSV MRQIHDKLGK
1060 1070 1080 1090 1100
DSVTQHNRSS QVASQLNFGE EAFKFDGPIS DRPMSNLEKV HFIVGYAIMR
1110 1120 1130 1140 1150
PGLRDEIYCQ ICKQLSENYK TSSRARGWIL LSLCLGCFPP SERFMKYLLN
1160 1170 1180 1190 1200
FISQGPPSYG PFCAERLQRT FANGVRAEPP TWLELQAVKS KKHIPIQVIL
1210 1220 1230 1240 1250
ATGRSLTISV DSASTSREIC QHVAQKQGLR DNLGFSLQVA VYDKFWSLGS
1260 1270 1280 1290 1300
GCDHLMDAVA QCEQLARERG ESQRQAPWRI YFRKEFFTPW HDSQEDPVST
1310 1320 1330 1340 1350
ELIYHQVLRG VWSGEYNFEK EEELVELLAR HCYVQLGATV KSNAVQELLP
1360 1370 1380 1390 1400
SCVPSKLYRT KSPEKWASLV TAAHAKAQYT QSKATPLAVR EQTVEAARLL
1410 1420 1430 1440 1450
WPLLFSRLFE VTTLSGPRLP KTQLVLAINW KGMYFLDQKE RTLLGLSFAE
1460 1470 1480 1490 1500
VMGLVANRDA PGGKKLLLAT LQEEYEFVSP SSVAIAEMVA LFLGGLKERS
1510 1520 1530 1540 1550
VFAMALQDRR ATDDITLLPF KKGDLLILTK KQGLLASENW ALGQNDRTGK
1560 1570 1580 1590 1600
TGLVPTACLY TIPSVTKPST QLLSLLAMSP EKRKLAAQEV RALEPPLEDQ
1610 1620 1630 1640 1650
LTESPYTLEE FSYQFFRAPE KETISRAAMP MARSRGHLWA YSPEPLRQPL
1660 1670 1680 1690 1700
LKSVHDKAKL RDAACQIFLA ILKYTGDYPS RQSWHSLELT DQMFSLALQD
1710 1720 1730 1740 1750
PALQDELYCQ ILKQLTHNSI RFSEERAWQL LWLCTGLFPP GKTLLPHAQK
1760 1770 1780 1790 1800
FIDSRKKKPL ALDCSRRLHR VLRVGPRKQP PHDVEVKAAE QNVSKLHHEV
1810 1820 1830 1840 1850
YLPNDTSKSM EVGSSSRVRD LCEGIGTRLQ LASWDGCSLF IKITDKVISL
1860 1870 1880 1890 1900
KEGDFFFDSL RQVSDWVKKN RPQKEGASVT LPYQVFFMRK LWLNVTPGKD
1910 1920 1930 1940 1950
VNADTILHYH QELPKYLRGF HKCSREDAIH LGGLICKIQF GSDSSQLASV
1960 1970 1980 1990 2000
SKVLKELVPQ NLTRLMSSEE WKKSLLLECD KNKRKTVAEA KVEFLKYMYR
2010 2020 2030 2040 2050
WPTFGSAFFE VKQTSEPSYP DILLIAINRH GLLLIHPKTK ELLNTYPFTK
2060 2070 2080 2090 2100
ISSWSSGNTY FHMALGSLGQ GSRLLCETSL GYKMDDLLTS YVQQLLNTVN
2110
KQRGFRAPAP ANP
Length:2,113
Mass (Da):240,859
Last modified:July 27, 2011 - v3
Checksum:i7116B047D1B08AA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti227 – 228IE → LA in AAK28339 (PubMed:11401444).Curated2
Sequence conflicti2033L → V in AAK28339 (PubMed:11401444).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242411 mRNA. Translation: AAK28339.1.
BC138338 mRNA. Translation: AAI38339.1.
BC138341 mRNA. Translation: AAI38342.1.
BC026416 mRNA. Translation: AAH26416.1.
AK220152 mRNA. Translation: BAD90338.1.
CCDSiCCDS50244.1.
RefSeqiNP_115770.2. NM_032394.3.
UniGeneiMm.440013.

Genome annotation databases

EnsembliENSMUST00000134663; ENSMUSP00000118046; ENSMUSG00000024388.
GeneIDi17922.
KEGGimmu:17922.
UCSCiuc008eix.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242411 mRNA. Translation: AAK28339.1.
BC138338 mRNA. Translation: AAI38339.1.
BC138341 mRNA. Translation: AAI38342.1.
BC026416 mRNA. Translation: AAH26416.1.
AK220152 mRNA. Translation: BAD90338.1.
CCDSiCCDS50244.1.
RefSeqiNP_115770.2. NM_032394.3.
UniGeneiMm.440013.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F3YX-ray3.41A962-1578[»]
ProteinModelPortaliQ99MZ6.
SMRiQ99MZ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99MZ6. 3 interactors.
MINTiMINT-4124477.
STRINGi10090.ENSMUSP00000118046.

PTM databases

iPTMnetiQ99MZ6.
PhosphoSitePlusiQ99MZ6.

Proteomic databases

MaxQBiQ99MZ6.
PaxDbiQ99MZ6.
PeptideAtlasiQ99MZ6.
PRIDEiQ99MZ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000134663; ENSMUSP00000118046; ENSMUSG00000024388.
GeneIDi17922.
KEGGimmu:17922.
UCSCiuc008eix.2. mouse.

Organism-specific databases

CTDi4648.
MGIiMGI:107709. Myo7b.

Phylogenomic databases

eggNOGiKOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129687.
HOGENOMiHOG000007836.
HOVERGENiHBG052557.
InParanoidiQ99MZ6.
KOiK10359.
OMAiKEFFTPW.
OrthoDBiEOG091G00BB.
PhylomeDBiQ99MZ6.
TreeFamiTF335306.

Miscellaneous databases

PROiQ99MZ6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024388.
GenevisibleiQ99MZ6. MM.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR000857. MyTH4_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001452. SH3_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00784. MyTH4. 2 hits.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00295. B41. 2 hits.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00139. MyTH4. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50057. FERM_3. 2 hits.
PS50096. IQ. 4 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51016. MYTH4. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO7B_MOUSE
AccessioniPrimary (citable) accession number: Q99MZ6
Secondary accession number(s): B9EHR5, Q571N7, Q8R0U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-7 (MYH7).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.