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Protein

Unconventional myosin-VIIb

Gene

Myo7b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. May link the complex to the actin core bundle of microvilli.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-VIIb
Gene namesi
Name:Myo7b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:107709. Myo7b.

Subcellular locationi

GO - Cellular componenti

  • apical cytoplasm Source: UniProtKB
  • brush border Source: UniProtKB
  • extracellular exosome Source: MGI
  • microvillus Source: UniProtKB
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21132113Unconventional myosin-VIIbPRO_0000329047Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei904 – 9041PhosphoserineCombined sources
Modified residuei1339 – 13391PhosphothreonineCombined sources
Modified residuei1368 – 13681PhosphoserineCombined sources
Modified residuei1642 – 16421PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99MZ6.
PaxDbiQ99MZ6.
PeptideAtlasiQ99MZ6.
PRIDEiQ99MZ6.

PTM databases

iPTMnetiQ99MZ6.
PhosphoSiteiQ99MZ6.

Expressioni

Tissue specificityi

Expressed primarily in kidney and intestine. Detected in proximal tubule cells of the kidney and enterocytes of the intestine, specifically the distal tips of apical microvilli on these transporting epithelial cells (at protein level).2 Publications

Developmental stagei

Detected in intestinal enterocytes at embryonic day 17.1 Publication

Gene expression databases

BgeeiENSMUSG00000024388.
GenevisibleiQ99MZ6. MM.

Interactioni

Subunit structurei

Part of the IMAC/intermicrovillar adhesion complex/intermicrovillar tip-link complex composed of ANKS4B, MYO7B, USH1C, CDHR2 and CDHR5. Interacts with CDHR2. Interacts with CDHR5. Interacts with USH1C (By similarity). Interacts with ANKS4B; requires initial interaction with USH1C (Probable).By similarity1 Publication

Protein-protein interaction databases

IntActiQ99MZ6. 3 interactions.
MINTiMINT-4124477.
STRINGi10090.ENSMUSP00000118046.

Structurei

Secondary structure

1
2113
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi973 – 9808Combined sources
Helixi1006 – 102015Combined sources
Helixi1086 – 109914Combined sources
Helixi1103 – 111513Combined sources
Helixi1121 – 113717Combined sources
Turni1142 – 11443Combined sources
Helixi1145 – 11539Combined sources
Turni1157 – 11593Combined sources
Helixi1160 – 117314Combined sources
Helixi1182 – 11909Combined sources
Beta strandi1194 – 12007Combined sources
Beta strandi1205 – 12106Combined sources
Helixi1216 – 122712Combined sources
Beta strandi1235 – 12417Combined sources
Beta strandi1244 – 12474Combined sources
Turni1249 – 12524Combined sources
Helixi1255 – 126915Combined sources
Turni1273 – 12753Combined sources
Beta strandi1278 – 12847Combined sources
Helixi1297 – 131317Combined sources
Beta strandi1314 – 13163Combined sources
Helixi1321 – 133515Combined sources
Helixi1342 – 135211Combined sources
Helixi1355 – 13584Combined sources
Helixi1363 – 137715Combined sources
Turni1378 – 13803Combined sources
Helixi1386 – 140015Combined sources
Turni1402 – 14054Combined sources
Beta strandi1406 – 141510Combined sources
Beta strandi1422 – 14287Combined sources
Beta strandi1433 – 14364Combined sources
Beta strandi1442 – 14465Combined sources
Helixi1448 – 14503Combined sources
Beta strandi1451 – 14566Combined sources
Beta strandi1465 – 14728Combined sources
Beta strandi1474 – 14785Combined sources
Helixi1482 – 149918Combined sources
Beta strandi1502 – 15054Combined sources
Beta strandi1525 – 15273Combined sources
Beta strandi1537 – 15459Combined sources
Turni1546 – 15483Combined sources
Beta strandi1551 – 15555Combined sources
Helixi1556 – 15583Combined sources
Beta strandi1559 – 15613Combined sources
Helixi1570 – 15756Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5F3YX-ray3.41A962-1578[»]
ProteinModelPortaliQ99MZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 760696Myosin motorAdd
BLAST
Domaini745 – 76521IQ 1PROSITE-ProRule annotationAdd
BLAST
Domaini763 – 79230IQ 2PROSITE-ProRule annotationAdd
BLAST
Domaini786 – 81530IQ 3PROSITE-ProRule annotationAdd
BLAST
Domaini809 – 83830IQ 4PROSITE-ProRule annotationAdd
BLAST
Domaini832 – 86130IQ 5PROSITE-ProRule annotationAdd
BLAST
Domaini855 – 88430IQ 6PROSITE-ProRule annotationAdd
BLAST
Domaini989 – 1189201MyTH4 1PROSITE-ProRule annotationAdd
BLAST
Domaini1194 – 1503310FERM 1PROSITE-ProRule annotationAdd
BLAST
Domaini1498 – 156467SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini1606 – 167065SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini1641 – 1790150MyTH4 2PROSITE-ProRule annotationAdd
BLAST
Domaini1790 – 1896107MyTH4 3PROSITE-ProRule annotationAdd
BLAST
Domaini1796 – 2099304FERM 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni962 – 1578617Mediates interaction with ANKS4B1 PublicationAdd
BLAST
Regioni1497 – 2113617Mediates interaction with CDHR2, CDHR5 and USH1CBy similarityAdd
BLAST

Sequence similaritiesi

Contains 2 FERM domains.PROSITE-ProRule annotation
Contains 6 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 3 MyTH4 domains.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129687.
HOGENOMiHOG000007836.
HOVERGENiHBG052557.
InParanoidiQ99MZ6.
KOiK10359.
OMAiKEFFTPW.
OrthoDBiEOG091G00BB.
PhylomeDBiQ99MZ6.
TreeFamiTF335306.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR000857. MyTH4_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001452. SH3_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00784. MyTH4. 2 hits.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00295. B41. 2 hits.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00139. MyTH4. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50057. FERM_3. 2 hits.
PS50096. IQ. 4 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51016. MYTH4. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99MZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFRLGDHV WLDPPSSSKT GVAIGGIVKE TKLGKTLIED DEGKEHWVHA
60 70 80 90 100
EDLSTLRPMH PNSAQGVDDM IRLGDLNEAG VVHNLLIRYQ QHKIYTYTGS
110 120 130 140 150
ILVAVNPFQM LPLYTLEQVQ IYYSRHMGEL PPHIFAIANS CYFNMKKNKR
160 170 180 190 200
DQCCIISGES GAGKTETTKL ILQFLATVSG QHSWIEQQVL EANPILEAFG
210 220 230 240 250
NAKTIRNDNS SRFGKYIDIH FNSSGVIEGA SIEHFLLEKS RVCRQAPEER
260 270 280 290 300
NYHIFYCMLM GMSPEEKQML SLGMPSEYHY LTMGSCTSSE GLSDAKDYAH
310 320 330 340 350
VRSAMKILQF SDSENWDISK LLAAILHLGN VGFMAAVFEN LDSSDVMETP
360 370 380 390 400
AFPLAMKLLE VQHQALRDCL IKHTIPVLGE FVSRPVNIAQ ATDRRDAFVK
410 420 430 440 450
GIYGRLFQWI VKKINAAIFT PQAQDPQNVR RAIGLLDIFG FENFQNNSFE
460 470 480 490 500
QLCINFANEH LQQFFVKHVF TMEQEEYLSE NITWNYIHYT DNQPILDMLA
510 520 530 540 550
LKPMSIISLL DEESRFPQGT DVTMLQKLNS IHANNKSFLS PRSIHDTRFG
560 570 580 590 600
IAHFAGDVYY QAEGFLEKNR DVLSTDILIL IHSSKNKFLK EIFNVDSSQT
610 620 630 640 650
KLGHGTICQV KAGSQLFKSS DSIKRPVTLA SQFKQSLDQL MRILTNCQPY
660 670 680 690 700
FVRCIKPNEY KKPLLFDREL CIQQLRYSGM METVHIRKSG FPIRYTFDEF
710 720 730 740 750
SQRFRVLLPS PERMQFQNKP RQMTLHIADL CLGTDKDWKV GKTKIFLKDH
760 770 780 790 800
QDTVLEIRRS QALDGAAIRI QRVLRGHKYR KEFLRQRRAA VTLQAGWRGY
810 820 830 840 850
SQRKNFKLIL VGFERLQAIA RSHLLMRQFQ AMRQRIVQLQ ARCRGYLVRQ
860 870 880 890 900
QVQAKRRAVV IIQAHARGMV VRKSYWQQKS TGPQVILAKE PKAQVAVHER
910 920 930 940 950
KRKSIYDTVT DTAMVEKVFG FLPAMIGGQE GPAPTRFEDL EVKTQKLHEV
960 970 980 990 1000
DLDTVPMMAM PEEEVDSLAE YTFPKFAVTY FQKSASHTHI QKPLRYPLLY
1010 1020 1030 1040 1050
HENDTDHSAA LDVWIIILRF MGDLPEPVVY GRNSLTGSSV MRQIHDKLGK
1060 1070 1080 1090 1100
DSVTQHNRSS QVASQLNFGE EAFKFDGPIS DRPMSNLEKV HFIVGYAIMR
1110 1120 1130 1140 1150
PGLRDEIYCQ ICKQLSENYK TSSRARGWIL LSLCLGCFPP SERFMKYLLN
1160 1170 1180 1190 1200
FISQGPPSYG PFCAERLQRT FANGVRAEPP TWLELQAVKS KKHIPIQVIL
1210 1220 1230 1240 1250
ATGRSLTISV DSASTSREIC QHVAQKQGLR DNLGFSLQVA VYDKFWSLGS
1260 1270 1280 1290 1300
GCDHLMDAVA QCEQLARERG ESQRQAPWRI YFRKEFFTPW HDSQEDPVST
1310 1320 1330 1340 1350
ELIYHQVLRG VWSGEYNFEK EEELVELLAR HCYVQLGATV KSNAVQELLP
1360 1370 1380 1390 1400
SCVPSKLYRT KSPEKWASLV TAAHAKAQYT QSKATPLAVR EQTVEAARLL
1410 1420 1430 1440 1450
WPLLFSRLFE VTTLSGPRLP KTQLVLAINW KGMYFLDQKE RTLLGLSFAE
1460 1470 1480 1490 1500
VMGLVANRDA PGGKKLLLAT LQEEYEFVSP SSVAIAEMVA LFLGGLKERS
1510 1520 1530 1540 1550
VFAMALQDRR ATDDITLLPF KKGDLLILTK KQGLLASENW ALGQNDRTGK
1560 1570 1580 1590 1600
TGLVPTACLY TIPSVTKPST QLLSLLAMSP EKRKLAAQEV RALEPPLEDQ
1610 1620 1630 1640 1650
LTESPYTLEE FSYQFFRAPE KETISRAAMP MARSRGHLWA YSPEPLRQPL
1660 1670 1680 1690 1700
LKSVHDKAKL RDAACQIFLA ILKYTGDYPS RQSWHSLELT DQMFSLALQD
1710 1720 1730 1740 1750
PALQDELYCQ ILKQLTHNSI RFSEERAWQL LWLCTGLFPP GKTLLPHAQK
1760 1770 1780 1790 1800
FIDSRKKKPL ALDCSRRLHR VLRVGPRKQP PHDVEVKAAE QNVSKLHHEV
1810 1820 1830 1840 1850
YLPNDTSKSM EVGSSSRVRD LCEGIGTRLQ LASWDGCSLF IKITDKVISL
1860 1870 1880 1890 1900
KEGDFFFDSL RQVSDWVKKN RPQKEGASVT LPYQVFFMRK LWLNVTPGKD
1910 1920 1930 1940 1950
VNADTILHYH QELPKYLRGF HKCSREDAIH LGGLICKIQF GSDSSQLASV
1960 1970 1980 1990 2000
SKVLKELVPQ NLTRLMSSEE WKKSLLLECD KNKRKTVAEA KVEFLKYMYR
2010 2020 2030 2040 2050
WPTFGSAFFE VKQTSEPSYP DILLIAINRH GLLLIHPKTK ELLNTYPFTK
2060 2070 2080 2090 2100
ISSWSSGNTY FHMALGSLGQ GSRLLCETSL GYKMDDLLTS YVQQLLNTVN
2110
KQRGFRAPAP ANP
Length:2,113
Mass (Da):240,859
Last modified:July 27, 2011 - v3
Checksum:i7116B047D1B08AA7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti227 – 2282IE → LA in AAK28339 (PubMed:11401444).Curated
Sequence conflicti2033 – 20331L → V in AAK28339 (PubMed:11401444).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242411 mRNA. Translation: AAK28339.1.
BC138338 mRNA. Translation: AAI38339.1.
BC138341 mRNA. Translation: AAI38342.1.
BC026416 mRNA. Translation: AAH26416.1.
AK220152 mRNA. Translation: BAD90338.1.
CCDSiCCDS50244.1.
RefSeqiNP_115770.2. NM_032394.3.
UniGeneiMm.440013.

Genome annotation databases

EnsembliENSMUST00000134663; ENSMUSP00000118046; ENSMUSG00000024388.
GeneIDi17922.
KEGGimmu:17922.
UCSCiuc008eix.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242411 mRNA. Translation: AAK28339.1.
BC138338 mRNA. Translation: AAI38339.1.
BC138341 mRNA. Translation: AAI38342.1.
BC026416 mRNA. Translation: AAH26416.1.
AK220152 mRNA. Translation: BAD90338.1.
CCDSiCCDS50244.1.
RefSeqiNP_115770.2. NM_032394.3.
UniGeneiMm.440013.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5F3YX-ray3.41A962-1578[»]
ProteinModelPortaliQ99MZ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99MZ6. 3 interactions.
MINTiMINT-4124477.
STRINGi10090.ENSMUSP00000118046.

PTM databases

iPTMnetiQ99MZ6.
PhosphoSiteiQ99MZ6.

Proteomic databases

MaxQBiQ99MZ6.
PaxDbiQ99MZ6.
PeptideAtlasiQ99MZ6.
PRIDEiQ99MZ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000134663; ENSMUSP00000118046; ENSMUSG00000024388.
GeneIDi17922.
KEGGimmu:17922.
UCSCiuc008eix.2. mouse.

Organism-specific databases

CTDi4648.
MGIiMGI:107709. Myo7b.

Phylogenomic databases

eggNOGiKOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129687.
HOGENOMiHOG000007836.
HOVERGENiHBG052557.
InParanoidiQ99MZ6.
KOiK10359.
OMAiKEFFTPW.
OrthoDBiEOG091G00BB.
PhylomeDBiQ99MZ6.
TreeFamiTF335306.

Miscellaneous databases

PROiQ99MZ6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024388.
GenevisibleiQ99MZ6. MM.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR000857. MyTH4_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001452. SH3_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00784. MyTH4. 2 hits.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00295. B41. 2 hits.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00139. MyTH4. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50057. FERM_3. 2 hits.
PS50096. IQ. 4 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51016. MYTH4. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO7B_MOUSE
AccessioniPrimary (citable) accession number: Q99MZ6
Secondary accession number(s): B9EHR5, Q571N7, Q8R0U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-7 (MYH7).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.